LOCUS       Exported                5121 bp ds-DNA     circular SYN 30-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 30, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      989..1617
                     /direction=RIGHT
                     /label=ColE1 origin
     misc_feature    2348..2773
                     /label=Tom22
     CDS             2792..3496
                     /label=mCherry
     rep_origin      3760..4200
                     /direction=RIGHT
                     /label=F1 ori
     promoter        4267..4295
                     /label=Amp prom
     promoter        4499..4695
                     /label=SV40 early promoter
     misc_feature    4546..4623
                     /label=SV40 ORI
ORIGIN
        1 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
       61 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
      121 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
      181 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
      241 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
      301 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
      361 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
      421 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
      481 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
      541 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
      601 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
      661 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
      721 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
      781 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
      841 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
      901 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      961 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1021 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1081 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1141 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     1201 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1261 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1321 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1381 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1441 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1501 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1561 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1621 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1681 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     1741 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1801 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1861 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1921 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1981 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2041 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2101 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2161 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2221 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2281 tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
     2341 cgaattcatg gctgccgccg tcgctgctgc cggtgcaggg gaaccccagt ccccggacga
     2401 attgctcccg aaaggcgacg cggagaagcc tgaggaggag ctggaggagg acgacgatga
     2461 ggagctagat gagaccctgt cggagagact atggggcctg acggagatgt ttccggagag
     2521 ggtccggtcc gcggccggag ccacttttga tctttccctc tttgtggctc agaaaatgta
     2581 caggttttcc agggcagcct tgtggattgg gaccacttcc tttatgatcc tggttcttcc
     2641 cgttgtcttt gagacggaga agttgcaaat ggagcaacag cagcaactgc agcagcggca
     2701 gatacttcta ggacctaaca cagggctctc aggaggaatg ccaggggctc taccctcact
     2761 tcctggaaag atcggaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc
     2821 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     2881 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     2941 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     3001 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     3061 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     3121 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     3181 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     3241 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     3301 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     3361 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     3421 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     3481 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
     3541 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3601 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3661 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3721 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
     3781 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     3841 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     3901 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     3961 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     4021 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     4081 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     4141 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     4201 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4261 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4321 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     4381 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4441 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4501 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4561 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4621 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4681 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4741 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4801 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4861 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     4921 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4981 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5041 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5101 tacgcttgat ccggctacct g
//
