LOCUS       Exported                7303 bp ds-DNA     circular SYN 30-JUN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 30, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
COMMENT     AssembledSeq
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    551..571
                     /label=pCMV F
     misc_feature    651..2069
                     /label=superYFP
     misc_feature    complement(840..860)
                     /label=superYFP seq R
     misc_feature    1372..1392
                     /label=superYFP seq F
     misc_feature    2085..3293
                     /label=AURKA FL
     misc_feature    complement(2284..2303)
                     
     CDS             2985..2996
                     /label=FactorXa site
     misc_feature    3303..4001
                     /label=mTurquoise2
     misc_feature    complement(3360..3376)
                     /label=pEGFP_N R
     misc_feature    3973..3991
                     /label=pEGFP_C F
     misc_feature    complement(4045..4064)
                     /label=pEGFP_Rev
     rep_origin      4261..4701
                     /direction=RIGHT
                     /label=F1 ori
     promoter        4768..4796
                     /label=Amp prom
     promoter        5000..5196
                     /label=SV40 early promoter
     misc_feature    5047..5124
                     /label=SV40 ORI
     CDS             5234..6025
                     /label=Kan/neoR
     rep_origin      6611..7239
                     /direction=RIGHT
                     /label=ColE1 origin
ORIGIN
        1 attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      421 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      481 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      541 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      601 ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag atggtctcca
      661 aaggagagga ggataacatg gcctctctcc cagcgacaca tgagttacac atctttggct
      721 ccttcaacgg tgtggacttt gacatggtgg gtcgtggcac cggcaatcca aatgatggtt
      781 atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc tggattctgg
      841 tccctcaaat cgggtatgga ttccatcagt acctgccctt ccccgacggg atgtcgcctt
      901 tccaggccgc catgaaagat ggctccggat accaagtcca tcgcacaatg cagtttgaag
      961 acggtgcctc cctgacttcc aactaccgct acacctacga gggaagccac atcaaaggag
     1021 agtttcaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc aactcgctga
     1081 ccgctgcgga ctggtgcgtg accaagatgc tgtaccccaa cgacaaaacc atcatcagca
     1141 cctttgactg gacttacacc actggaaatg gcaagcgcta ccagagcact gcgcggacca
     1201 cctacacctt tgccaagcca atggcggcca acatcctgaa gaaccagccg atgttcgtgt
     1261 tccgtaagac ggaactcaag cactccaaga ccgaactcaa cttcaaggag tggcaaaagg
     1321 catttaccga tgtgatgtcg actggcactg gttctacggg ctcgggctcc tcaggagagg
     1381 aggataacat ggcctctctc ccagcgacac atgagttaca catctttggc tccttcaacg
     1441 gtgtggactt tgacatggtg ggtcgtggca ccggcaatcc aaatgatggt tatgaggagt
     1501 taaacctgaa gtccaccaag ggtgacctcc agttctcccc ctggattctg gtccctcaaa
     1561 tcgggtatgg cttccatcag tacctgccct tccccgacgg gatgtcgcct ttccaggccg
     1621 ccatgaaaga tggctccgga taccaagtcc atcgcacaat gcagtttgaa gacggtgcct
     1681 ccctgacttc caactaccgc tacacctacg agggaagcca catcaaagga gagtttcagg
     1741 tgaaggggac tggtttccct gctgacggtc ctgtgatgac caactcgctg accgctgcgg
     1801 actggtgcgt gaccaagatg ctgtacccca acgacaaaac catcatcagc acctttgact
     1861 ggacttacac cactggaaat ggcaagcgct accagagcac tgcgcggacc acctacacct
     1921 ttgccaagcc aatggcggcc aacatcctga agaaccagcc gatgttcgtg ttccgtaaga
     1981 cggaactcaa gcactccaag accgaactca acttcaagga gtggcaaaag gcatttaccg
     2041 atgtgatggg catggacgag ctgtacaagg gaagcttcga attcatggac cgatctaaag
     2101 aaaactgcat ttcaggacct gttaaggcta cagctccagt tggaggtcca aaacgtgttc
     2161 tcgtgactca gcaatttcct tgtcagaatc cattacctgt aaatagtggc caggctcagc
     2221 gggtcttgtg tccttcaaat tcttcccagc gcattccttt gcaagcacaa aaacttgtct
     2281 ccagtcacaa gccggttcag aatcagaagc agaagcaatt gcaggcaacc agtgtacctc
     2341 atcctgtctc caggccactg aataacaccc aaaagagcaa gcagcccctg ccatcggcac
     2401 ctgaaaataa tcctgaggag gaactggcat caaaacagaa aaatgaagaa tcaaaaaaga
     2461 ggcagtgggc tttggaagac tttgaaattg gtcgccctct gggtaaagga aagtttggta
     2521 atgtttattt ggcaagagaa aagcaaagca agtttattct ggctcttaaa gtgttattta
     2581 aagctcagct ggagaaagcc ggagtggagc atcagctcag aagagaagta gaaatacagt
     2641 cccaccttcg gcatcctaat attcttagac tgtatggtta tttccatgat gctaccagag
     2701 tctacctaat tctggaatat gcaccacttg gaacagttta tagagaactt cagaaacttt
     2761 caaagtttga tgagcagaga actgctactt atataacaga attggcaaat gcactatcat
     2821 attgccattc gaagagagtt attcatagag acattaagcc agagaactta cttcttggat
     2881 cagctggaga gcttaaaatt gcagattttg ggtggtcagt acatgctcca tcttccagga
     2941 ggaccactct ctgtggcacc ctggactacc tgccccctga aatgattgaa ggtcggatgc
     3001 atgatgagaa ggtggatctc tggagccttg gagttctttg ctatgaattt ttagttggga
     3061 agcctccttt tgaggcaaac acataccaag agacctacaa aagaatatca cgggttgagt
     3121 tcacattccc tgactttgta acagagggag ccagggacct catttcaaga ctgttgaagc
     3181 ataatcccag ccagaggcca atgctcagag aagtacttga acacccctgg atcacagcaa
     3241 attcatcaaa accatcaaat tgccaaaaca aagaatcagc tagcaaacag tctggggatc
     3301 cgatggcggc cgcaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3361 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3421 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     3481 tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc
     3541 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3601 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     3661 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     3721 acatcctggg gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg
     3781 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     3841 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3901 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     3961 gcgatcacat ggtcctgctg gagttcgtga ccgccgccta agcggccgcg actctagatc
     4021 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4081 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4141 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4201 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag
     4261 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     4321 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     4381 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     4441 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     4501 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     4561 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4621 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     4681 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     4741 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4801 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     4861 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4921 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4981 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5041 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5101 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5161 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     5221 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5281 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5341 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5401 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5461 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5521 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5581 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5641 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5701 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     5761 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5821 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5881 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5941 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6001 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6061 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6121 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6181 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     6241 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     6301 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     6361 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     6421 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     6481 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6541 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctg
//
