LOCUS       G1333_DddAtox-C–        8559 bp    DNA     circular UNA 01-JUL-2020
DEFINITION  NLS-DddE(85-178aa)-32aa linker- dspCas9-UGI-UGI-NLS.
ACCESSION   urn.local...x04-8letjr8
VERSION     urn.local...x04-8letjr8
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    10..13
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    24..29
                     /Recognition_pattern="GACGT^C"
                     /annotation_group="GACGTC"
                     /label="AatII"
                     /note="Geneious type: restriction site"
     misc_feature    43..46
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    44..54
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    49..53
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    49..53
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    65..68
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    98..101
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    127..132
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     misc_feature    149..152
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    177..181
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    210..215
                     /Recognition_pattern="GACGT^C"
                     /annotation_group="GACGTC"
                     /label="AatII"
                     /note="Geneious type: restriction site"
     primer_bind     219..243
                     /Sequence="GGGAGTTTGTTTTGGCACCAAAATC"
                     /Hairpin_Tm="50.7"
                     /Tm="61.9"
                     /Self_Dimer_Tm="25.8"
                     /%GC="44.0"
                     /created_by="primer3"
                     /label="BM244"
     primer_bind     234..253
                     /Sequence="CACCAAAATCAACGGGACTT"
                     /Transferred_From="LCv2_seq1"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq1"
     primer_bind     261..285
                     /Sequence="TGTCGTAACAACTCCGCCCCATTGA"
                     /Hairpin_Tm="30.7"
                     /Tm="66.4"
                     /Self_Dimer_Tm="None"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="ACK15"
     primer_bind     261..285
                     /Sequence="TGTCGTAACAACTCCGCCCCATTGA"
                     /Transferred_From="ACK15"
                     /Transferred_Similarity="100.00%"
                     /label="ACK15"
     primer_bind     261..285
                     /Sequence="TGTCGTAACAACTCCGCCCCATTGA"
                     /Transferred_From="JLseq007_ACK15"
                     /Transferred_Similarity="100.00%"
                     /label="JLseq007_ACK15"
     Promoter        275..356
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="CMV prom"
                     /Transferred_Similarity="97.56%"
                     /label="CMV prom"
     primer_bind     286..306
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /Transferred_From="Genewiz CMV-Forward"
                     /Transferred_Similarity="100.00%"
                     /label="Genewiz CMV-Forward"
     primer_bind     286..306
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /Transferred_From="Eton CMV-Forward"
                     /Transferred_Similarity="100.00%"
                     /label="Eton CMV-Forward"
     primer_bind     286..306
                     /Sequence="CGCAAATGGGCGGTAGGCGTG"
                     /Transferred_From="QB0012 CMV Forward"
                     /Transferred_Similarity="100.00%"
                     /label="QB0012 CMV Forward"
     primer_bind     293..314
                     /Sequence="GGGCGGTAGGCGTGTACGGTGG"
                     /Transferred_From="QB0976 pRLuc-MCS-F"
                     /Transferred_Similarity="100.00%"
                     /label="QB0976 pRLuc-MCS-F"
     misc_feature    306..309
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(331..360)
                     /Sequence="AGCGGATCTGACGGTTCACTAAACCAGCTC"
                     /Transferred_From="352_CMV_Rev2"
                     /Transferred_Similarity="100.00%"
                     /label="352_CMV_Rev2"
     misc_feature    332..335
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    353..356
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    364..367
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    369..376
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    371..374
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     Promoter        377..395
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     overhang        399..410
                     /created_by="bmok"
                     /label="user junction"
     primer_bind     399..415
                     /Sequence="AGCCGCCACCATGAAAC"
                     /Hairpin_Tm="None"
                     /Tm="57.9"
                     /Self_Dimer_Tm="None"
                     /%GC="58.8"
                     /modified_by="bmok"
                     /label="BM536"
     motif           403..408
                     /created_by="bmok"
                     /label="Kozak sequence "
     CDS             409..465
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="BPNLS"
     misc_feature    437..440
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    439..443
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     motif           complement(445..465)
                     /created_by="bmok"
                     /label="annealing region "
     misc_feature    complement(445..475)
                     /Sequence="ACGGGGTTGGGACTTTCCGCTTCTTCTTTGG"
                     /Hairpin_Tm="37.4"
                     /Tm="71.7"
                     /Self_Dimer_Tm="None"
                     /%GC="54.8"
                     /created_by="primer3"
                     /label="BM551"
                     /note="Geneious type: primer_bind_reverse"
     overhang        462..475
                     /created_by="bmok"
                     /label="user junction "
     primer_bind     462..483
                     /Sequence="AGTCCCAACCCCGTACCCAAAC"
                     /Hairpin_Tm="None"
                     /Tm="64.5"
                     /Self_Dimer_Tm="None"
                     /%GC="59.1"
                     /created_by="primer3"
                     /label="BM550 "
     CDS             <466..747
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="DddA(1334-1427)"
     regulatory      499..501
                     /created_by="bmok"
                     /label="His96-chelate to zinc"
     misc_feature    505..507
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="E1298A mutant catalytically dead "
     regulatory      583..585
                     /created_by="bmok"
                     /label="C124 chelate to zinc "
     misc_feature    complement(731..750)
                     /Sequence="AGAGCAACCGCCTTTGGTCG"
                     /Hairpin_Tm="51.3"
                     /Tm="63.3"
                     /Self_Dimer_Tm="15.0"
                     /%GC="60.0"
                     /created_by="primer3"
                     /label="BM452"
                     /note="Geneious type: primer_bind_reverse"
     trimmed         733..>747
                     /created_by="bmok"
                     /label
     overhang        738..750
                     /created_by="bmok"
                     /label="user junction "
     primer_bind     738..767
                     /created_by="bmok"
                     /Sequence="AGGCGGTTGCTCTGGAGGATCTAGCGGAGG"
                     /Hairpin_Tm="39.9"
                     /Tm="73.5"
                     /Self_Dimer_Tm="12.8"
                     /%GC="63.3"
                     /created_by="primer3"
                     /modified_by="bmok"
                     /label="BM451"
     CDS             748..843
                     /created_by="jonlevy"
                     /Transferred_From="JL545-pCMV_BPNLS_rAPOBEC1(genscript)_dl
                     BE4_NpuN1"
                     /Transferred_Similarity="100.00%"
                     /label="Genscript BE4 linker"
     misc_feature    755..758
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    766..771
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    767..770
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    787..791
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    787..791
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    799..803
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(831..853)
                     /Sequence="ACTTCTTGTCGCTGCCGCCGCTG"
                     /Hairpin_Tm="37.0"
                     /Tm="70.2"
                     /Self_Dimer_Tm="5.2"
                     /%GC="65.2"
                     /created_by="primer3"
                     /label="BM545 "
                     /note="Geneious type: primer_bind_reverse"
     overhang        841..853
                     /created_by="bmok"
                     /label="user junction "
     primer_bind     841..864
                     /Sequence="AGCGACAAGAAGTACAGCATCGGC"
                     /Hairpin_Tm="41.6"
                     /Tm="65.5"
                     /Self_Dimer_Tm="None"
                     /%GC="54.2"
                     /modified_by="bmok"
                     /label="BM544 (can use as sequencing primer) "
     CDS             844..4944
                     /note="hSpCas9"
                     /modified_by="jonlevy"
                     /label="fz hSpCas9"
     misc_feature    852..855
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    862..865
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    864..868
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    864..868
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    866..871
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI"
                     /note="Geneious type: restriction site"
     misc_feature    867..870
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     motif           868..870
                     /created_by="bmok"
                     /label="D10A "
     misc_feature    complement(868..886)
                     /Sequence="CAGAGTTGGTGCCGATGGC"
                     /Hairpin_Tm="50.9"
                     /Tm="61.4"
                     /Self_Dimer_Tm="None"
                     /%GC="63.2"
                     /created_by="primer3"
                     /label="BM447 "
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    894..897
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    900..903
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    912..915
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    957..960
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     primer_bind     965..985
                     /Sequence="GCATCAAGAAGAACCTGATCG"
                     /Transferred_From="LCv2_seq10"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq10"
     primer_bind     981..1002
                     /Sequence="GATCGGAGCCCTGCTGTTCGAC"
                     /Hairpin_Tm="39.9"
                     /Tm="65.4"
                     /Self_Dimer_Tm="None"
                     /%GC="63.6"
                     /created_by="primer3"
                     /label="BM245"
     misc_feature    981..984
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    998..1001
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    1020..1023
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1026..1029
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    1071..1074
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    1074..1077
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    1091..1096
                     /Recognition_pattern="A^GATCT"
                     /annotation_group="AGATCT"
                     /label="BglII"
                     /note="Geneious type: restriction site"
     misc_feature    1092..1095
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    1109..1114
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI"
                     /note="Geneious type: restriction site"
     misc_feature    1110..1113
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1126..1129
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    1134..1144
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     misc_feature    1147..1151
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    1155..1159
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    1155..1159
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    1218..1221
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1233..1236
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    1284..1287
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1299..1302
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    1308..1311
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1311..1315
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    1311..1315
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    1314..1317
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1323..1326
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    1332..1335
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    1336..1339
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1347..1350
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    1349..1352
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    1388..1391
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    1399..1404
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    1400..1403
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     primer_bind     1417..1436
                     /Sequence="AACCAGCTGTTCGAGGAAAA"
                     /Transferred_From="LCv2_seq11"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq11"
     misc_feature    1420..1425
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    1421..1424
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    1427..1430
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    1467..1470
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1515..1518
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    1522..1527
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    1523..1526
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    1529..1532
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    1546..1549
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1575..1579
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    1575..1579
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    1579..1582
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1607..1610
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    1611..1615
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    1611..1615
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    1614..1617
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1630..1635
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    1633..1638
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    1634..1637
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    1662..1666
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    1662..1666
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    1677..1680
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1683..1686
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    1695..1698
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    1713..1716
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1773..1776
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    1782..1785
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    1792..1797
                     /Recognition_pattern="RGCGC^Y"
                     /annotation_group="RGCGCY"
                     /label="HaeII"
                     /note="Geneious type: restriction site"
     misc_feature    1793..1796
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    1803..1806
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    1827..1831
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    1827..1831
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    1830..1834
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     misc_feature    1848..1851
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    1864..1869
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    1865..1868
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    1878..1881
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    1895..1898
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    1918..1923
                     /Recognition_pattern="GCC^GGC"
                     /annotation_group="GCCGGC"
                     /label="NaeI"
                     /note="Geneious type: restriction site"
     misc_feature    1919..1922
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    1944..1948
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    1944..1948
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     primer_bind     1969..1988
                     /Sequence="AAGCCCATCCTGGAAAAGAT"
                     /Transferred_From="LCv2_seq12"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq12"
     misc_feature    1977..1981
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    1977..1981
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    2015..2018
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    2028..2032
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     misc_feature    2048..2052
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     misc_feature    2054..2057
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    2079..2082
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    2085..2089
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    2085..2089
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    2093..2096
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    2148..2151
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    2157..2160
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    2159..2162
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    2166..2169
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    2197..2200
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    2204..2209
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI"
                     /note="Geneious type: restriction site"
     misc_feature    2205..2208
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    2207..2211
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    2207..2211
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    2222..2226
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    2228..2232
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    2228..2232
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    2264..2268
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    2264..2268
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    2273..2276
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    2293..2298
                     /Recognition_pattern="RGCGC^Y"
                     /annotation_group="RGCGCY"
                     /label="HaeII"
                     /note="Geneious type: restriction site"
     misc_feature    2294..2297
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    2308..2311
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    2315..2318
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    2333..2336
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    2382..2385
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    2387..2392
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI"
                     /note="Geneious type: restriction site"
     misc_feature    2388..2391
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    2408..2411
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     primer_bind     2468..2486
                     /Sequence="AGCAGAAAAAGGCCATCGT"
                     /Transferred_From="LCv2_seq13"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq13"
     misc_feature    2478..2481
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    2487..2491
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     misc_feature    2508..2511
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    2527..2532
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    2528..2531
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(2529..2579)
                     /Sequence="ATTTCCACGGAGTCGAAGCACTCGATTTTCTTGAAGTAGTCCTCTTT
                     CAGC"
                     /%GC="45.1"
                     /Transferred_From="876_fzCas9-resplice"
                     /Transferred_Similarity="100.00%"
                     /label="876_fzCas9-resplice"
     primer_bind     complement(2529..2559)
                     /Sequence="CTCGATTTTCTTGAAGTAGTCCTCTTTCAGC"
                     /%GC="41.9"
                     /Transferred_From="901_fzCas9_NpuC-rev"
                     /Transferred_Similarity="100.00%"
                     /label="901_fzCas9_NpuC-rev"
     misc_feature    2555..2558
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     primer_bind     2560..2582
                     /Sequence="TGCTTCGACTCCGTGGAAATCTC"
                     /%GC="52.2"
                     /Transferred_From="841_FZ_Cas9-C11-fwd"
                     /Transferred_Similarity="100.00%"
                     /label="841_FZ_Cas9-C11-fwd"
     misc_feature    2564..2567
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    2566..2570
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    2582..2585
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    2593..2596
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    2610..2614
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    2610..2614
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    2626..2629
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    2658..2662
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    2658..2662
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    2692..2697
                     /Recognition_pattern="GAT^ATC"
                     /annotation_group="GATATC"
                     /label="EcoRV"
                     /note="Geneious type: restriction site"
     misc_feature    2733..2736
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    2735..2738
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    2768..2771
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    2788..2793
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    2789..2792
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    2810..2813
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    2829..2832
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    2834..2837
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    2838..2841
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    2850..2853
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    2864..2867
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    2877..2881
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    2877..2881
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    2923..2928
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    2924..2927
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    2928..2931
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    2950..2955
                     /Recognition_pattern="TTT^AAA"
                     /annotation_group="TTTAAA"
                     /label="DraI"
                     /note="Geneious type: restriction site"
     misc_feature    2951..2954
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     primer_bind     2962..2981
                     /Sequence="ATCCAGAAAGCCCAGGTGTC"
                     /Transferred_From="LCv2_seq14"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq14"
     misc_feature    2973..2977
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    2973..2977
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    2981..2984
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    2983..2986
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    2985..2989
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    2985..2989
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    3013..3023
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    3021..3024
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    3022..3027
                     /Recognition_pattern="GCC^GGC"
                     /annotation_group="GCCGGC"
                     /label="NaeI"
                     /note="Geneious type: restriction site"
     misc_feature    3023..3026
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    3038..3041
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    3052..3057
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    3076..3081
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI, SstI"
                     /note="Geneious type: restriction site"
     misc_feature    3077..3080
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3094..3097
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    3095..3100
                     /Recognition_pattern="GCC^GGC"
                     /annotation_group="GCCGGC"
                     /label="NaeI"
                     /note="Geneious type: restriction site"
     misc_feature    3096..3099
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    3106..3111
                     /Recognition_pattern="C^YCGRG"
                     /annotation_group="CYCGRG"
                     /label="AvaI"
                     /note="Geneious type: restriction site"
     misc_feature    3120..3123
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    3122..3125
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    3128..3133
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI"
                     /note="Geneious type: restriction site"
     misc_feature    3129..3132
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    3189..3192
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    3191..3194
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    3209..3212
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3222..3225
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    3253..3258
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    3254..3257
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3256..3261
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    3269..3272
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3273..3276
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    3279..3282
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    3286..3291
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    3306..3309
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    3312..3316
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     misc_feature    3315..3325
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     misc_feature    3315..3319
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    3315..3319
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    3333..3336
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     modified_base   3358..3360
                     /created_by="bmok"
                     /label="H840A"
     misc_feature    3373..3376
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3388..3392
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    3395..3398
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    3429..3432
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     primer_bind     3455..3474
                     /Sequence="CCGAAGAGGTCGTGAAGAAG"
                     /Transferred_From="LCv2_seq15"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq15"
     misc_feature    3493..3498
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    3494..3497
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3509..3512
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3530..3533
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    3546..3549
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    3559..3562
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    3579..3582
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    3580..3585
                     /Recognition_pattern="GCC^GGC"
                     /annotation_group="GCCGGC"
                     /label="NaeI"
                     /note="Geneious type: restriction site"
     misc_feature    3581..3584
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    3598..3603
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    3599..3602
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3612..3615
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    3618..3621
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    3639..3642
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    3642..3646
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    3642..3646
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    3646..3650
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    3651..3654
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    3666..3669
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    3683..3686
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3687..3690
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    3690..3693
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    3705..3708
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    3722..3725
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3738..3741
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    3765..3768
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    3771..3774
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    3831..3834
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    3840..3843
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    3848..3851
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3867..3870
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    3885..3888
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    3903..3906
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     primer_bind     3920..3938
                     /Sequence="AGGAAATCGGCAAGGCTAC"
                     /Transferred_From="LCv2_seq16"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq16"
     misc_feature    3944..3949
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI"
                     /note="Geneious type: restriction site"
     misc_feature    3945..3948
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    3993..3997
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    3993..3997
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    3995..4000
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI"
                     /note="Geneious type: restriction site"
     misc_feature    3996..3999
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4008..4011
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4011..4014
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    4019..4022
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4026..4029
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4028..4031
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    4046..4049
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    4053..4056
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4069..4072
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4071..4074
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    4103..4108
                     /Recognition_pattern="GCATG^C"
                     /annotation_group="GCATGC"
                     /label="SphI"
                     /note="Geneious type: restriction site"
     misc_feature    4162..4166
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    4193..4196
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    4197..4200
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4218..4222
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     misc_feature    4230..4233
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    4241..4244
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    4257..4260
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4277..4282
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI"
                     /note="Geneious type: restriction site"
     misc_feature    4278..4281
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4325..4328
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    4335..4338
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4357..4360
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    4361..4364
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    4368..4372
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    4376..4379
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    4416..4420
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     misc_feature    4422..4425
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4430..4433
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    4439..4444
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI"
                     /note="Geneious type: restriction site"
     misc_feature    4440..4443
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    4451..4454
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    4454..4457
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    4465..4468
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4467..4470
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     primer_bind     4470..4489
                     /Sequence="GAAGAGAATGCTGGCCTCTG"
                     /Transferred_From="LCv2_seq17"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq17"
     misc_feature    4482..4485
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4489..4494
                     /Recognition_pattern="GCC^GGC"
                     /annotation_group="GCCGGC"
                     /label="NaeI"
                     /note="Geneious type: restriction site"
     misc_feature    4490..4493
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    4498..4503
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    4518..4521
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4548..4551
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    4551..4555
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    4551..4555
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    4553..4558
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI"
                     /note="Geneious type: restriction site"
     misc_feature    4554..4557
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4571..4574
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    4585..4590
                     /Recognition_pattern="C^YCGRG"
                     /annotation_group="CYCGRG"
                     /label="AvaI"
                     /note="Geneious type: restriction site"
     misc_feature    4606..4611
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    4607..4610
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     primer_bind     4632..4650
                     /Sequence="CTACCTGGACGAGATCATC"
                     /Hairpin_Tm="None"
                     /Tm="53.9"
                     /Self_Dimer_Tm="None"
                     /%GC="52.6"
                     /created_by="primer3"
                     /label="BM254"
     misc_feature    4635..4639
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    4635..4639
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    4644..4647
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4649..4652
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    4656..4659
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4680..4683
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4683..4687
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    4683..4687
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    4686..4689
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4731..4734
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    4755..4758
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4814..4819
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI"
                     /note="Geneious type: restriction site"
     misc_feature    4815..4818
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    4832..4835
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    4836..4839
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    4845..4848
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    4881..4884
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4898..4901
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    4900..4903
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4905..4908
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    4917..4920
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    4919..4922
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(4921..4960)
                     /Sequence="CGCCGCTCCCGCCGCTGTCACCTCCCAGCTGAGACAGGTC"
                     /%GC="72.5"
                     /Transferred_From="871_fzCas9/GenscriptUGI-rev"
                     /Transferred_Similarity="100.00%"
                     /label="871_fzCas9/GenscriptUGI-rev"
     misc_feature    4930..4935
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    4931..4934
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     primer_bind     4941..4960
                     /Sequence="TGACAGCGGCGGGAGCGGCG"
                     /%GC="80.0"
                     /Transferred_From="868_Genscript_UGI-fwd"
                     /Transferred_Similarity="100.00%"
                     /label="868_Genscript_UGI-fwd"
     source          4945..5514
                     /created_by="jonlevy"
                     /modified_by="jonlevy"
                     /label="Genscript 2xUGI"
     CDS             4975..5223
                     /created_by="kzhao"
                     /label="UGI"
     misc_feature    5014..5019
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    5015..5018
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    5029..5033
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    5067..5070
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    5083..5087
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    5094..5098
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    5094..5098
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    5098..5103
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     misc_feature    5116..5120
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    5158..5163
                     /Recognition_pattern="C^YCGRG"
                     /annotation_group="CYCGRG"
                     /label="AvaI"
                     /note="Geneious type: restriction site"
     misc_feature    5175..5178
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    5178..5182
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    5178..5182
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    5187..5191
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    5187..5191
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    5191..5195
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    5211..5214
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    5230..5235
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    5231..5234
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    5234..5237
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    5240..5243
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     CDS             5254..5502
                     /created_by="kzhao"
                     /label="UGI"
     misc_feature    5273..5276
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5289..5308)
                     /Sequence="CCTGGATGACCAGCTGCTTG"
                     /Hairpin_Tm="56.0"
                     /Tm="61.0"
                     /Self_Dimer_Tm="8.7"
                     /%GC="60.0"
                     /created_by="primer3"
                     /label="BM448 "
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5293..5298
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    5294..5297
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    5304..5308
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    5304..5308
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    5336..5339
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    5346..5349
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    5368..5373
                     /Recognition_pattern="GAT^ATC"
                     /annotation_group="GATATC"
                     /label="EcoRV"
                     /note="Geneious type: restriction site"
     misc_feature    5373..5377
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    5373..5377
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    5376..5380
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     misc_feature    5399..5402
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    5450..5454
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    5450..5454
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    5466..5470
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    5466..5470
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    5470..5474
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     CDS             5515..5565
                     /created_by="kzhao"
                     /label="BPNLS"
     misc_feature    5536..5541
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    5540..5543
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5544..>5565)
                     /Sequence="ATGTTAGCAGACTTCCCCTGCCCTCGCCGCTGCCGACTTTCCTCTTC
                     TTCTTGGGC"
                     /created_by="bmok"
                     /label="BM508"
                     /note="Geneious type: primer_bind_reverse"
     overhang        <5566..5573
                     /created_by="bmok"
                     /label="user junction "
     misc_feature    5569..5572
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    5596..5601
                     /Recognition_pattern="TTT^AAA"
                     /annotation_group="TTTAAA"
                     /label="DraI"
                     /note="Geneious type: restriction site"
     misc_feature    5597..5600
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    5608..5611
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(5614..5631)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Transferred_From="Eton BGH Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="Eton BGH Reverse"
     misc_feature    5616..5619
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     Terminator      5617..5844
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="bGH_PA term"
                     /Transferred_Similarity="100.00%"
                     /label="bGH_PA term"
     misc_feature    5678..5682
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    5678..5682
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    complement(5766..5790)
                     /Sequence="CCTTGCTGTCCTGCCCCACCCCACC"
                     /Hairpin_Tm="None"
                     /Tm="73.0"
                     /Self_Dimer_Tm="None"
                     /%GC="72.0"
                     /created_by="primer3"
                     /label="ACK64"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(5766..5790)
                     /Sequence="CCTTGCTGTCCTGCCCCACCCCACC"
                     /Transferred_From="ACK64 (end)"
                     /Transferred_Similarity="100.00%"
                     /label="ACK64 (end)"
     misc_feature    5802..5807
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="BbsI restriction site (staggered cut on both
                     strands) "
     misc_feature    5816..5821
                     /Recognition_pattern="GCATG^C"
                     /annotation_group="GCATGC"
                     /label="SphI"
                     /note="Geneious type: restriction site"
     misc_feature    5864..5869
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    5865..5868
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    5874..5877
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    5882..5887
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     misc_feature    5882..5887
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    5882..5887
                     /Recognition_pattern="GT^MKAC"
                     /annotation_group="GTMKAC"
                     /label="AccI"
                     /note="Geneious type: restriction site"
     misc_feature    5883..5886
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    5892..5895
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    5898..5901
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(5915..5931)
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="Eton M13R Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="Eton M13R Reverse"
     misc_feature    5920..5923
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(5928..5950)
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Transferred_From="QB0050 M13/pUC Reverse"
                     /Transferred_Similarity="100.00%"
                     /label="QB0050 M13/pUC Reverse"
     Promoter        complement(5964..5993)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lac prom"
                     /Transferred_Similarity="100.00%"
                     /label="lac prom"
     misc_feature    5974..5977
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(6008..6032)
                     /Sequence="AATTAATGTGAGTTAGCTCACTCAT"
                     /Hairpin_Tm="58.9"
                     /Tm="56.6"
                     /Self_Dimer_Tm="15.0"
                     /%GC="32.0"
                     /created_by="primer3"
                     /label="ACK32"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     6008..6032
                     /Sequence="ATGAGTGAGCTAACTCACATTAATT"
                     /Hairpin_Tm="63.4"
                     /Tm="56.6"
                     /Self_Dimer_Tm="30.8"
                     /%GC="32.0"
                     /created_by="primer3"
                     /label="ACK21"
     misc_feature    6015..6018
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    6027..6030
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    6038..6041
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(6040..6056)
                     /Sequence="GAAAGCGGGCAGTGAGC"
                     /Hairpin_Tm="44.7"
                     /Tm="58.7"
                     /Self_Dimer_Tm="None"
                     /%GC="64.7"
                     /created_by="primer3"
                     /label="BM524"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    6078..6083
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     misc_feature    6079..6082
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    6086..6089
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    6091..6095
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    6096..6099
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    6103..6106
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    6130..6135
                     /Recognition_pattern="RGCGC^Y"
                     /annotation_group="RGCGCY"
                     /label="HaeII"
                     /note="Geneious type: restriction site"
     misc_feature    6131..6134
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    6156..6160
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    6164..6167
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    6197..6200
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(6198..6217)
                     /created_by="primer3"
                     /annotation_group="13547385713896020002e37130e-0600-4795-b
                     4cf-db36738ae7a4"
                     /%GC="50.0"
                     /Tm="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATTACCGCCTTTGAGTGAGC"
                     /modified_by="User"
                     /Transferred_From="VR"
                     /Transferred_Similarity="100.00%"
                     /label="VR"
     misc_feature    6231..6235
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    6270..6273
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    6281..6284
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    6283..6287
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    6283..6287
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     primer_bind     complement(6287..6308)
                     /Sequence="CAACGCGGCCTTTTTACGGTTC"
                     /Transferred_From="11_pFYF_Seq_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="11_pFYF_Seq_Rev"
     misc_feature    6299..6302
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    complement(6302..6921)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.52%"
                     /label="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     primer_bind     6317..6341
                     /Sequence="TTTCCATAGGCTCCGCCCCCCTGAC"
                     /Hairpin_Tm="52.7"
                     /Tm="69.9"
                     /Self_Dimer_Tm="None"
                     /%GC="64.0"
                     /created_by="primer3"
                     /label="ACK10"
     misc_feature    6356..6359
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(6378..6397)
                     /Sequence="TATAGTCCTGTCGGGTTTCG"
                     /Transferred_From="BR322-F"
                     /Transferred_Similarity="100.00%"
                     /label="BR322-F"
     misc_feature    6404..6408
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    6404..6408
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    6417..6421
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    6417..6421
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    6423..6426
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    6434..6437
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    6463..6466
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    6500..6505
                     /Recognition_pattern="RGCGC^Y"
                     /annotation_group="RGCGCY"
                     /label="HaeII"
                     /note="Geneious type: restriction site"
     misc_feature    6501..6504
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    6513
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    6513..6516
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     primer_bind     6540..6558
                     /Sequence="GTGTAGGTCGTTCGCTCCA"
                     /Transferred_From="QB0766 RepO-F"
                     /Transferred_Similarity="100.00%"
                     /label="QB0766 RepO-F"
     misc_feature    6559..6562
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    6570..6575
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     misc_feature    6601..6604
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    6610..6613
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    6627..6631
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    6636..6639
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     primer_bind     6637..6656
                     /Sequence="CGGTAAGACACGACTTATCG"
                     /%GC="50.0"
                     /Transferred_From="833_BR322-rev1"
                     /Transferred_Similarity="100.00%"
                     /label="833_BR322-rev1"
     primer_bind     complement(6665..6684)
                     /Sequence="TCCTGTTACCAGTGGCTGCT"
                     /Transferred_From="QB0767 RepO-R"
                     /Transferred_Similarity="100.00%"
                     /label="QB0767 RepO-R"
     misc_feature    6733..6736
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    6758
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6775..6778
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    6816..6819
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     primer_bind     6818..6842
                     /Sequence="CTCTTGATCCGGCAAACAAACCACC"
                     /Hairpin_Tm="None"
                     /Tm="64.7"
                     /Self_Dimer_Tm="2.9"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="ACK11"
     primer_bind     complement(6818..6837)
                     /Sequence="TTTGTTTGCCGGATCAAGAG"
                     /%GC="45.0"
                     /Transferred_From="832_BR322-fwd1"
                     /Transferred_Similarity="100.00%"
                     /label="832_BR322-fwd1"
     misc_feature    6823..6826
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    6826..6829
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    6884..6887
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    6898..6901
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    6909..6912
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    6917..6920
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    6962..6965
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    6995..6998
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    7007..7010
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    7013..7018
                     /Recognition_pattern="TTT^AAA"
                     /annotation_group="TTTAAA"
                     /label="DraI"
                     /note="Geneious type: restriction site"
     misc_feature    7014..7017
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    7019..7022
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    7032..7037
                     /Recognition_pattern="TTT^AAA"
                     /annotation_group="TTTAAA"
                     /label="DraI"
                     /note="Geneious type: restriction site"
     misc_feature    7033..7036
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     Marker          complement(7076..7936)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /label="amp marker"
     misc_feature    7086..7089
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    7112..7115
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    7144..7148
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    7191..7194
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    7230..7233
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    7263..7273
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    7264..7267
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    7271..7274
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    7277..7280
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    7287..7291
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     primer_bind     7317..7341
                     /Sequence="TCTATTAATTGTTGCCGGGAAGCTA"
                     /Hairpin_Tm="None"
                     /Tm="60.4"
                     /Self_Dimer_Tm="9.1"
                     /%GC="40.0"
                     /created_by="primer3"
                     /label="ACK12"
     misc_feature    7321..7324
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    7331..7334
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    7337..7340
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    7360..7363
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    7370..7373
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    7437..7440
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    7441..7444
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     misc_feature    7453..7456
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    7471..7474
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     primer_bind     7482..7501
                     /Sequence="TTGTGCAAAAAAGCGGTTAG"
                     /Transferred_From="ACK563"
                     /Transferred_Similarity="100.00%"
                     /label="ACK563"
     misc_feature    7500..7503
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    7509..7513
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     misc_feature    7516..7521
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI"
                     /note="Geneious type: restriction site"
     misc_feature    7517..7520
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    7538..7541
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    7627..7632
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI"
                     /note="Geneious type: restriction site"
     misc_feature    7628..7631
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    7683..7686
                     /Recognition_pattern="C^CGG"
                     /annotation_group="CCGG"
                     /label="HpaII, MspI"
                     /note="Geneious type: restriction site"
     primer_bind     7699..7718
                     /Sequence="ATAATACCGCGCCACATAGC"
                     /Transferred_From="QB0005 Amp-R"
                     /Transferred_Similarity="100.00%"
                     /label="QB0005 Amp-R"
     misc_feature    7707..7710
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    7724..7729
                     /Recognition_pattern="TTT^AAA"
                     /annotation_group="TTTAAA"
                     /label="DraI"
                     /note="Geneious type: restriction site"
     misc_feature    7725..7728
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    7775..7778
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     primer_bind     7784..7811
                     /Sequence="GCTGTTGAGATCCAGTTCGATGTAACCC"
                     /Transferred_From="563_Amp_Rev"
                     /Transferred_Similarity="100.00%"
                     /label="563_Amp_Rev"
     primer_bind     complement(7784..7811)
                     /Sequence="GGGTTACATCGAACTGGATCTCAACAGC"
                     /Transferred_From="562_Amp_Fwd"
                     /Transferred_Similarity="100.00%"
                     /label="562_Amp_Fwd"
     misc_feature    7792..7795
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    7800..7803
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    7816..7821
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     primer_bind     7817..7841
                     /Sequence="TGCACCCAACTGATCTTCAGCATCT"
                     /Hairpin_Tm="50.2"
                     /Tm="64.5"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /label="ACK13"
     misc_feature    7828..7831
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Promoter        complement(7978..8006)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     misc_feature    8039..8042
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     primer_bind     8050..8067
                     /Sequence="CCCGAAAAGTGCCACCTG"
                     /Transferred_From="QB0090 pQE promoter"
                     /Transferred_Similarity="100.00%"
                     /label="QB0090 pQE promoter"
     misc_feature    8067..8072
                     /Recognition_pattern="GACGT^C"
                     /annotation_group="GACGTC"
                     /label="AatII"
                     /note="Geneious type: restriction site"
     misc_feature    8070..8075
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     misc_feature    8070..8075
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    8070..8075
                     /Recognition_pattern="GT^MKAC"
                     /annotation_group="GTMKAC"
                     /label="AccI"
                     /note="Geneious type: restriction site"
     misc_feature    8071..8074
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    8077..8080
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    8085..8088
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    8086..8091
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI"
                     /note="Geneious type: restriction site"
     misc_feature    8087..8090
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    8089..8092
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    8097..8100
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     misc_feature    8108..8113
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     misc_feature    8108..8113
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    8108..8113
                     /Recognition_pattern="GT^MKAC"
                     /annotation_group="GTMKAC"
                     /label="AccI"
                     /note="Geneious type: restriction site"
     misc_feature    8109..8112
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    8111..8115
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    8119..8122
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(8120..8139)
                     /Sequence="CGGCATCAGAGCAGATTGTA"
                     /Transferred_From="QB0092 pRS-marker"
                     /Transferred_Similarity="100.00%"
                     /label="QB0092 pRS-marker"
     misc_feature    8145..8148
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    8194..8197
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    8207..8210
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    8210..8213
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    8249..8253
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     misc_feature    8274..8277
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     misc_feature    8282..8287
                     /Recognition_pattern="TCG^CGA"
                     /annotation_group="TCGCGA"
                     /label="NruI"
                     /note="Geneious type: restriction site"
     primer_bind     8285..8305
                     /Sequence="CGATGTACGGGCCAGATATAC"
                     /Hairpin_Tm="38.5"
                     /Tm="57.9"
                     /Self_Dimer_Tm="10.9"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="KZ887"
     misc_feature    8289..8292
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    8294..8297
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    8308..8313
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     primer_bind     8320..8344
                     /Sequence="TATTGACTAGTTATTAATAGTAATC"
                     /Hairpin_Tm="32.1"
                     /Tm="48.0"
                     /Self_Dimer_Tm="5.9"
                     /%GC="20.0"
                     /created_by="primer3"
                     /label="ACK14"
     primer_bind     8320..8344
                     /Sequence="TATTGACTAGTTATTAATAGTAATC"
                     /Transferred_From="JLseq006_ACK14"
                     /Transferred_Similarity="100.00%"
                     /label="JLseq006_ACK14"
     promoter        8327..356
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="CMV_Full"
                     /Transferred_Similarity="99.83%"
                     /label="CMV_Full"
     primer_bind     8327..8370
                     /Sequence="TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCC"
                     /Transferred_From="387_pAAV_fwd"
                     /Transferred_Similarity="100.00%"
                     /label="387_pAAV_fwd"
     misc_feature    8333..8336
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     primer_bind     8371..8388
                     /Sequence="CATATATGGAGTTCCGCG"
                     /Hairpin_Tm="39.4"
                     /Tm="52.5"
                     /Self_Dimer_Tm="4.7"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="BM243"
     primer_bind     complement(8377..8396)
                     /Sequence="TTATGTAACGCGGAACTCCA"
                     /Transferred_From="QB1110 pSicoR"
                     /Transferred_Similarity="100.00%"
                     /label="QB1110 pSicoR"
     misc_feature    8409..8412
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    8410..8420
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    8415..8419
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    8415..8419
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    8447..8452
                     /Recognition_pattern="GACGT^C"
                     /annotation_group="GACGTC"
                     /label="AatII"
                     /note="Geneious type: restriction site"
     misc_feature    8500..8505
                     /Recognition_pattern="GACGT^C"
                     /annotation_group="GACGTC"
                     /label="AatII"
                     /note="Geneious type: restriction site"
     misc_feature    8516
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8532..8542
                     /Recognition_pattern="GCCNNNN^NGGC"
                     /annotation_group="GCCNNNNNGGC"
                     /label="BglI"
                     /note="Geneious type: restriction site"
     misc_feature    8544..8547
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     misc_feature    8559..5
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcccaac cccgtaccca
      481 aactatgcca atgccggtca tgtggagggt cagagcgccc tgttcatgcg tgataacggc
      541 atcagcgagg gtctggtgtt ccacaacaac ccggaaggca cctgcggttt ttgcgtgaac
      601 atgaccgaga ccctgctgcc ggaaaacgcg aaaatgaccg tggtgccgcc ggaaggtgcc
      661 attccagtga agcgcggcgc taccggtgaa accaaagtgt ttaccggtaa cagcaacagc
      721 ccgaagagcc cgaccaaagg cggttgctct ggaggatcta gcggaggatc ctctggcagc
      781 gagacaccag gaacaagcga gtcagcaaca ccagagagca gtggcggcag cagcggcggc
      841 agcgacaaga agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg
      901 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
      961 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
     1021 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
     1081 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
     1141 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     1201 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     1261 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     1321 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     1381 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     1441 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     1501 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac
     1561 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     1621 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     1681 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     1741 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     1801 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     1861 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     1921 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     1981 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     2041 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     2101 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     2161 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     2221 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     2281 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     2341 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     2401 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     2461 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     2521 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     2581 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     2641 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     2701 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     2761 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     2821 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     2881 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     2941 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     3001 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     3061 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     3121 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     3181 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     3241 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     3301 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggacgct
     3361 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     3421 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     3481 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     3541 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     3601 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     3661 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     3721 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     3781 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     3841 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     3901 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     3961 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     4021 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     4081 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     4141 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
     4201 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     4261 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     4321 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     4381 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     4441 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     4501 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     4561 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     4621 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     4681 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     4741 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     4801 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     4861 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     4921 gacctgtctc agctgggagg tgacagcggc gggagcggcg ggagcggggg gagcactaat
     4981 ctgagcgaca tcattgagaa ggagactggg aaacagctgg tcattcagga gtccatcctg
     5041 atgctgcctg aggaggtgga ggaagtgatc ggcaacaagc cagagtctga catcctggtg
     5101 cacaccgcct acgacgagtc cacagatgag aatgtgatgc tgctgacctc tgacgccccc
     5161 gagtataagc cttgggccct ggtcatccag gattctaacg gcgagaataa gatcaagatg
     5221 ctgagcggag gatccggagg atctggaggc agcaccaacc tgtctgacat catcgagaag
     5281 gagacaggca agcagctggt catccaggag agcatcctga tgctgcccga agaagtcgaa
     5341 gaagtgatcg gaaacaagcc tgagagcgat atcctggtcc ataccgccta cgacgagagt
     5401 accgacgaaa atgtgatgct gctgacatcc gacgccccag agtataagcc ctgggctctg
     5461 gtcatccagg attccaacgg agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga
     5521 accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtctaacc ggtcatcatc
     5581 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     5641 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5701 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5761 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5821 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     5881 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     6001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6061 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6721 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8041 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
     8101 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     8161 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     8221 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     8281 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     8341 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     8401 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     8461 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     8521 tacggtaaac tgcccacttg gcagtacatc aagtgtatc
//
