LOCUS       G1333_DddAtox-N–        7185 bp    DNA     circular UNA 01-JUL-2020
DEFINITION  from ACK174 but genscript 32aa, DddE(41-84)-32aa linker-saC9
            D10A-UGI-UGI-NLS.
ACCESSION   urn.local...1e-c3z4jul
VERSION     urn.local...1e-c3z4jul
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     236..256
                     /Sequence="CCAAAATCAACGGGACTTTCC"
                     /Hairpin_Tm="35.2"
                     /Tm="57.7"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /label="BM596"
     primer_bind     261..285
                     /Sequence="TGTCGTAACAACTCCGCCCCATTGA"
                     /Hairpin_Tm="30.7"
                     /Tm="66.4"
                     /Self_Dimer_Tm="None"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="ACK15"
     Promoter        275..356
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="CMV prom"
                     /Transferred_Similarity="97.56%"
                     /label="CMV prom"
     Promoter        377..395
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7 prom"
                     /Transferred_Similarity="100.00%"
                     /label="T7 prom"
     primer_bind     377..396
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Hairpin_Tm="None"
                     /Tm="50.3"
                     /Self_Dimer_Tm="None"
                     /%GC="40.0"
                     /created_by="primer3"
                     /label="T7"
     motif           complement(396..411)
                     /created_by="bmok"
                     /label="annealing "
     misc_feature    complement(396..418)
                     /Sequence="AGCTGCCCATGGTGGCGGCTCTC"
                     /Hairpin_Tm="74.7"
                     /Tm="71.4"
                     /Self_Dimer_Tm="32.1"
                     /%GC="69.6"
                     /created_by="primer3"
                     /label="BM598"
                     /note="Geneious type: primer_bind_reverse"
     overhang        406..418
                     /created_by="bmok"
                     /label="user junction "
     primer_bind     406..427
                     /Sequence="ACCATGGGCAGCTACGCCCTGG"
                     /Hairpin_Tm="65.5"
                     /Tm="69.1"
                     /Self_Dimer_Tm="30.5"
                     /%GC="68.2"
                     /modified_by="bmok"
                     /label="BM597"
     CDS             412..543
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="DddA(1290-1333)"
     polylinker      439..480
                     /created_by="bmok"
                     /label="loop, might be involved in dsDNA binding "
     misc_feature    508..510
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="Split_G73"
                     /note="Geneious type: Sequence difference"
     misc_feature    complement(528..546)
                     /Sequence="AGAACCGCCGCTGCTAAAC"
                     /Hairpin_Tm="34.4"
                     /Tm="61.0"
                     /Self_Dimer_Tm="None"
                     /%GC="57.9"
                     /created_by="primer3"
                     /label="BM549"
                     /note="Geneious type: primer_bind_reverse"
     overhang        535..546
                     /created_by="bmok"
                     /label="user junction"
     primer_bind     535..583
                     /created_by="bmok"
                     /Sequence="AGCGGCGGTTCTGGTGGTTCTTCTGGTGGTTCTAGCGGCAGCGAGAC
                     TC"
                     /modified_by="bmok"
                     /label="BM631"
     primer_bind     535..562
                     /Sequence="AGCGGCGGTTCTGGTGGTTCTTCTGGTG"
                     /Hairpin_Tm="None"
                     /Tm="72.1"
                     /Self_Dimer_Tm="5.4"
                     /%GC="60.7"
                     /created_by="primer3"
                     /label="BM603"
     misc_feature    541..543
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="Split G84"
                     /note="Geneious type: Sequence difference"
     motif           544..562
                     /created_by="bmok"
                     /label="annealing "
     CDS             544..639
                     /created_by="User"
                     /modified_by="bmok"
                     /label="32 aa linker"
     misc_feature    581..595
                     /Sequence="CTCCCGGGACCTCAG"
                     /Hairpin_Tm="None"
                     /Tm="54.0"
                     /Self_Dimer_Tm="22.6"
                     /%GC="73.3"
                     /created_by="primer3"
                     /label="BM586"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(599..618)
                     /Sequence="ACTACTTTCGGGTGTGGCGG"
                     /Hairpin_Tm="None"
                     /Tm="62.4"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /label="BM624"
                     /note="Geneious type: primer_bind_reverse"
     overhang        606..618
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="user junction "
     primer_bind     606..661
                     /Sequence="ACCCGAAAGTAGTGGCGGCAGCAGCGGCGGCAGCGGGAAACGGAACT
                     ACATCCTGG"
                     /created_by="bmok"
                     /label="BM623"
     CDS             <640..3897
                     /created_by="User"
                     /modified_by="User"
                     /modified_by="bmok"
                     /label="hSaCas9-KKH"
     misc_feature    complement(<640..659)
                     /Sequence="AGGATGTAGTTCCGTTTCCCAGAACCACCAGAAGAACCACC"
                     /created_by="User"
                     /label="ACK1224"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     643..664
                     /Sequence="AAACGGAACTACATCCTGGGGC"
                     /Hairpin_Tm="37.3"
                     /Tm="62.6"
                     /Self_Dimer_Tm="None"
                     /%GC="54.5"
                     /created_by="primer3"
                     /label="ACK1223"
     modified_base   667..669
                     /created_by="User"
                     /label="D10A"
     misc_difference 667..669
                     /created_by="bmok"
                     /label="D10A"
     primer_bind     681..700
                     /Sequence="CAGCGTTGGCTACGGAATTA"
                     /Transferred_From="saCas9_seq1"
                     /Transferred_Similarity="100.00%"
                     /label="saCas9_seq1"
     misc_feature    complement(711..730)
                     /Sequence="CGTCAATCACATCGCGTGTC"
                     /Hairpin_Tm="46.4"
                     /Tm="60.0"
                     /Self_Dimer_Tm="5.8"
                     /%GC="55.0"
                     /created_by="primer3"
                     /label="BM589"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    856..865
                     /Recognition_pattern="GATNN^NNATC"
                     /annotation_group="GATNNNNATC"
                     /label="BsaBI, Bse8I, BseJI"
                     /note="Geneious type: restriction site"
     primer_bind     1166..1185
                     /Sequence="ACGTAAAGGAAGCCAAGCAG"
                     /Transferred_From="saCas9_seq2"
                     /Transferred_Similarity="100.00%"
                     /label="saCas9_seq2"
     misc_feature    1278..1283
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    1278..1283
                     /Recognition_pattern="G^GGCCC"
                     /annotation_group="GGGCCC"
                     /label="Bsp120I, PspOMI"
                     /note="Geneious type: restriction site"
     misc_feature    1281..1287
                     /Recognition_pattern="CC^CWGGG"
                     /annotation_group="CCCWGGG"
                     /label="PasI"
                     /note="Geneious type: restriction site"
     misc_feature    1359..1364
                     /Recognition_pattern="T^CCGGA"
                     /annotation_group="TCCGGA"
                     /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI"
                     /note="Geneious type: restriction site"
     primer_bind     1398..1416
                     /Sequence="CGACCTGTACAATGCCCTC"
                     /Hairpin_Tm="None"
                     /Tm="57.9"
                     /Self_Dimer_Tm="None"
                     /%GC="57.9"
                     /created_by="primer3"
                     /modified_by="bmok"
                     /label="BM588"
     misc_feature    1458..1470
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1482..1507)
                     /Sequence="GCTTAAACACATTCTCGATAATCTGG"
                     /Hairpin_Tm="None"
                     /Tm="58.7"
                     /Self_Dimer_Tm="4.3"
                     /%GC="38.5"
                     /created_by="primer3"
                     /label="BM587"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1525..1530
                     /Recognition_pattern="C^TTAAG"
                     /annotation_group="CTTAAG"
                     /label="AflII, BfrI, BspTI, BstAFI, MspCI, Vha464I"
                     /note="Geneious type: restriction site"
     primer_bind     1644..1663
                     /Sequence="AACCGCACGAAAGGAGATAA"
                     /Transferred_From="saCas9_seq3"
                     /Transferred_Similarity="100.00%"
                     /label="saCas9_seq3"
     primer_bind     2166..2185
                     /Sequence="AAGAACAACCGGCAAAGAGA"
                     /Transferred_From="saCas9_seq4"
                     /Transferred_Similarity="100.00%"
                     /label="saCas9_seq4"
     misc_feature    2184..2189
                     /Recognition_pattern="GAATGC(1/-1)"
                     /annotation_group="GAATGC"
                     /label="BsmI, Mva1269I, PctI"
                     /note="Geneious type: restriction site"
     misc_feature    2217..2227
                     /Recognition_pattern="GCANNNN^NTGC"
                     /annotation_group="GCANNNNNTGC"
                     /label="BstAPI"
                     /note="Geneious type: restriction site"
     primer_bind     2235..2254
                     /Sequence="CAAGTGCCTGTACTCTCTGG"
                     /Hairpin_Tm="None"
                     /Tm="57.6"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /label="BM591"
     misc_feature    complement(2316..2337)
                     /Sequence="GTCAAAGCTTACGGATCTAGGG"
                     /Hairpin_Tm="None"
                     /Tm="58.3"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="BM590"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2328..2333
                     /Recognition_pattern="A^AGCTT"
                     /annotation_group="AAGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     misc_feature    2616..2622
                     /Recognition_pattern="CC^TNAGG"
                     /annotation_group="CCTNAGG"
                     /label="AxyI, Bse21I, Bsu36I, Eco81I"
                     /note="Geneious type: restriction site"
     primer_bind     2661..2680
                     /Sequence="GAGCATAAATGGGGGATTCA"
                     /Transferred_From="saCas9_seq5"
                     /Transferred_Similarity="100.00%"
                     /label="saCas9_seq5"
     misc_feature    complement(2743..2749)
                     /Recognition_pattern="CCTCAGC(-5/-2)"
                     /annotation_group="CCTCAGC"
                     /label="BbvCI"
                     /note="Geneious type: restriction site"
     modified_base   2983..2985
                     /created_by="bmok"
                     /label="E782K"
     primer_bind     3102..3121
                     /Sequence="CAAGTCTCCAGAGAAGCTCC"
                     /Hairpin_Tm="39.5"
                     /Tm="57.3"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /label="BM593"
     primer_bind     complement(3168..3187)
                     /Sequence="CATCCCCGTATTGCTCCATG"
                     /Hairpin_Tm="None"
                     /Tm="58.5"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /label="BM592"
     primer_bind     3174..3193
                     /Sequence="GCAATACGGGGATGAGAAGA"
                     /Transferred_From="saCas9_seq6"
                     /Transferred_Similarity="100.00%"
                     /label="saCas9_seq6"
     misc_feature    3192..3204
                     /Recognition_pattern="(7/12)GAACNNNNNNTAC(12/7)"
                     /annotation_group="GAACNNNNNNTAC"
                     /label="PsrI"
                     /note="Geneious type: restriction site"
     misc_feature    3302..3309
                     /Recognition_pattern="RTGC^GCAY"
                     /annotation_group="RTGCGCAY"
                     /label="FspAI"
                     /note="Geneious type: restriction site"
     modified_base   3541..3543
                     /created_by="bmok"
                     /label="N968K"
     modified_base   3682..3684
                     /created_by="bmok"
                     /label="R1015H"
     misc_feature    3799..3804
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     misc_feature    3825..3830
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI, PaeR7I, Sfr274I, SlaI"
                     /note="Geneious type: restriction site"
     primer_bind     3849..3874
                     /Sequence="CGGTGATTATAAAGATCATGACATCG"
                     /Hairpin_Tm="36.1"
                     /Tm="58.3"
                     /Self_Dimer_Tm="None"
                     /%GC="38.5"
                     /created_by="primer3"
                     /label="BM595"
     overhang        <3898..>3909
                     /label="misc"
     CDS             <3910..4158
                     /created_by="User"
                     /label="UGI"
     misc_feature    complement(4016..4036)
                     /Sequence="GCACGAGTATATCGCTTTCCG"
                     /Hairpin_Tm="56.3"
                     /Tm="59.3"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="BM594"
                     /note="Geneious type: primer_bind_reverse"
     CDS             4171..4191
                     /created_by="User"
                     /modified_by="bmok"
                     /label="SV40 NLS"
     Terminator      4243..4470
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="bGH_PA term"
                     /Transferred_Similarity="100.00%"
                     /label="bGH_PA term"
     misc_feature    complement(4392..4416)
                     /Sequence="CCTTGCTGTCCTGCCCCACCCCACC"
                     /Hairpin_Tm="None"
                     /Tm="73.0"
                     /Self_Dimer_Tm="None"
                     /%GC="72.0"
                     /created_by="primer3"
                     /label="ACK64"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4496..4513)
                     /Sequence="GTCGACGGTATCGAGCCC"
                     /Hairpin_Tm="59.6"
                     /Tm="59.7"
                     /Self_Dimer_Tm="0.3"
                     /%GC="66.7"
                     /created_by="primer3"
                     /label="BM596"
                     /note="Geneious type: primer_bind_reverse"
     Promoter        complement(4590..4619)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="lac prom"
                     /Transferred_Similarity="100.00%"
                     /label="lac prom"
     misc_feature    complement(4634..4658)
                     /Sequence="AATTAATGTGAGTTAGCTCACTCAT"
                     /Hairpin_Tm="58.9"
                     /Tm="56.6"
                     /Self_Dimer_Tm="15.0"
                     /%GC="32.0"
                     /created_by="primer3"
                     /label="ACK32"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     4634..4658
                     /Sequence="ATGAGTGAGCTAACTCACATTAATT"
                     /Hairpin_Tm="63.4"
                     /Tm="56.6"
                     /Self_Dimer_Tm="30.8"
                     /%GC="32.0"
                     /created_by="primer3"
                     /label="ACK21"
     misc_feature    complement(4666..4682)
                     /Sequence="GAAAGCGGGCAGTGAGC"
                     /Hairpin_Tm="44.7"
                     /Tm="58.7"
                     /Self_Dimer_Tm="None"
                     /%GC="64.7"
                     /created_by="primer3"
                     /label="BM524 "
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4928..5547)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="99.52%"
                     /label="pBR322 origin"
                     /note="Geneious type: Origin of Replication"
     primer_bind     4943..4967
                     /Sequence="TTTCCATAGGCTCCGCCCCCCTGAC"
                     /Hairpin_Tm="52.7"
                     /Tm="69.9"
                     /Self_Dimer_Tm="None"
                     /%GC="64.0"
                     /created_by="primer3"
                     /label="ACK10"
     misc_feature    5137^5138
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    5139
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    5384
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     5444..5468
                     /Sequence="CTCTTGATCCGGCAAACAAACCACC"
                     /Hairpin_Tm="None"
                     /Tm="64.7"
                     /Self_Dimer_Tm="2.9"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="ACK11"
     Marker          complement(5702..6562)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /label="amp marker"
     primer_bind     5943..5967
                     /Sequence="TCTATTAATTGTTGCCGGGAAGCTA"
                     /Hairpin_Tm="None"
                     /Tm="60.4"
                     /Self_Dimer_Tm="9.1"
                     /%GC="40.0"
                     /created_by="primer3"
                     /label="ACK12"
     primer_bind     6443..6467
                     /Sequence="TGCACCCAACTGATCTTCAGCATCT"
                     /Hairpin_Tm="50.2"
                     /Tm="64.5"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /label="ACK13"
     Promoter        complement(6604..6632)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     primer_bind     6911..6931
                     /Sequence="CGATGTACGGGCCAGATATAC"
                     /Hairpin_Tm="38.5"
                     /Tm="57.9"
                     /Self_Dimer_Tm="10.9"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="BM525"
     primer_bind     6946..6970
                     /Sequence="TATTGACTAGTTATTAATAGTAATC"
                     /Hairpin_Tm="32.1"
                     /Tm="48.0"
                     /Self_Dimer_Tm="5.9"
                     /%GC="20.0"
                     /created_by="primer3"
                     /label="ACK14"
     misc_feature    7142
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gggcagctac
      421 gccctgggtc cgtatcagat tagcgccccg cagctgccag cttacaatgg tcagaccgtg
      481 ggtaccttct actatgtgaa cgacgcgggc ggtctggaga gcaaggtgtt tagcagcggc
      541 ggttctggtg gttcttctgg tggttctagc ggcagcgaga ctcccgggac ctcagagtcc
      601 gccacacccg aaagtagtgg cggcagcagc ggcggcagcg ggaaacggaa ctacatcctg
      661 gggcttgcca ttgggataac cagcgttggc tacggaatta ttgattatga gacacgcgat
      721 gtgattgacg ccggggttag gctgttcaaa gaggccaacg ttgaaaacaa cgagggaaga
      781 cggagtaagc gcggagcaag aagactcaag cgcagacgga gacatcggat tcagagggtg
      841 aaaaagctgc tcttcgatta caatctcctg accgatcata gtgagctgag cggaatcaac
      901 ccctacgagg cgcgagtgaa agggctttcc cagaagctgt ccgaagagga gttctccgcc
      961 gcgttgctgc acctggccaa acggaggggg gttcacaatg taaacgaagt ggaggaggac
     1021 acgggcaatg aacttagtac gaaagaacag atcagtagga actctaaggc tctcgaagag
     1081 aaatacgtcg ctgagttgca gcttgagaga ctgaaaaaag acggcgaagt acgcggatct
     1141 attaataggt tcaagacttc agattacgta aaggaagcca agcagctcct gaaagtacag
     1201 aaagcgtacc atcagctcga tcagagcttc atcgatacct acatagattt gctggagaca
     1261 cggaggacat actacgaggg cccaggggaa ggatctcctt ttgggtggaa ggacatcaag
     1321 gaatggtacg agatgcttat gggacattgt acatattttc cggaggagct caggagcgtc
     1381 aagtacgcct acaatgccga cctgtacaat gccctcaatg acctcaataa cctcgtgatt
     1441 accagggacg agaacgagaa gctggagtac tatgaaaagt tccagattat cgagaatgtg
     1501 tttaagcaga agaagaagcc gacacttaag cagattgcaa aggaaatcct cgtgaatgag
     1561 gaagatatca agggatacag agtgacaagt acaggcaagc ccgagttcac aaatctgaag
     1621 gtgtaccacg atattaagga cataaccgca cgaaaggaga taatcgaaaa cgctgagctc
     1681 ctcgatcaga tcgcaaaaat tcttaccatc taccagtcta gtgaggacat tcaggaggaa
     1741 ctgactaatc tgaacagtga gctcacccaa gaggaaattg agcagatttc aaacctgaaa
     1801 ggctacaccg ggacgcacaa tctgagcctc aaagcaatca acctcattct ggatgaactt
     1861 tggcacacaa atgacaacca aattgccata ttcaaccgcc tgaaactggt gccaaaaaaa
     1921 gtggatctgt cacagcaaaa ggaaatccct acaaccttgg ttgacgattt tattctgtcc
     1981 cccgttgtca agcggagctt catccagtca atcaaggtga tcaatgccat cattaaaaaa
     2041 tacggattgc caaacgatat aattatcgag cttgcacgag agaagaactc aaaggacgcc
     2101 cagaagatga ttaacgaaat gcagaagcgc aaccgccaga caaacgaacg catagaggaa
     2161 attataagaa caaccggcaa agagaatgcc aagtatctga tcgagaaaat caagctgcac
     2221 gacatgcaag aaggcaagtg cctgtactct ctggaagcta tcccactcga agatctgctg
     2281 aataatccat tcaattacga ggtggaccac atcatcccta gatccgtaag ctttgacaat
     2341 tccttcaata acaaagttct ggttaaacag gaggaaaatt ctaaaaaagg gaaccggacc
     2401 ccgttccagt acctgagctc cagtgacagc aagattagct acgagacttt taagaaacat
     2461 attctgaatc tggccaaagg caaaggcagg atcagcaaga ccaagaagga gtacctcctc
     2521 gaagaacgcg acattaacag atttagtgtg cagaaagatt tcatcaaccg aaaccttgtc
     2581 gatactcggt acgccacgag aggcctgatg aatctcctca ggagctactt ccgcgtcaat
     2641 aatctggacg ttaaagtcaa gagcataaat gggggattca ccagctttct gaggagaaag
     2701 tggaagttta agaaggaacg aaacaaagga tacaagcacc atgctgagga tgctttgatc
     2761 atcgctaacg cggactttat ctttaaggaa tggaaaaagc tggataaggc aaagaaagtg
     2821 atggaaaacc agatgttcga ggagaagcag gcagagtcaa tgcctgagat cgagacagag
     2881 caggaataca aggaaatttt catcacccct catcagatta aacacataaa ggacttcaaa
     2941 gactataaat actctcatag ggtggacaaa aaacccaatc gcaagctcat taatgacacc
     3001 ctgtactcaa cacggaagga tgataaaggt aataccttga ttgtgaataa tcttaatgga
     3061 ttgtatgaca aagataacga caagctcaag aagctgatca acaagtctcc agagaagctc
     3121 cttatgtatc accacgaccc acagacttat cagaaattga aactgatcat ggagcaatac
     3181 ggggatgaga agaacccact ctacaaatat tatgaggaaa caggtaatta cctgaccaag
     3241 tactccaaga aggataacgg accagtgatc aaaaagataa agtactatgg caacaaactt
     3301 aatgcgcatt tggacataac tgacgattac cccaattctc gaaacaaggt tgtgaagctc
     3361 tccctgaagc cttatagatt tgacgtgtac ctggataatg gggtttataa attcgtcacc
     3421 gtgaaaaatc tggacgtgat caaaaaggag aactattatg aagtaaactc aaagtgctat
     3481 gaggaggcga agaagctgaa gaagatctcc aatcaggccg agttcatcgc ttccttctat
     3541 aagaacgatc tcatcaagat caatggagag ctttatcgcg tcattggtgt gaacaatgac
     3601 ttgctgaaca ggatcgaagt caatatgata gacattacct accgggagta tctcgaaaac
     3661 atgaatgata aacggccgcc tcacatcatc aagacaatcg catctaaaac tcagtcaata
     3721 aaaaagtact ctaccgatat cctggggaat ctctatgaag tgaagtcaaa gaagcaccca
     3781 caaatcatta aaaaaggtgg atcccccaag aagaagagga aagtctcgag cgactacaaa
     3841 gaccatgacg gtgattataa agatcatgac atcgattaca aggatgacga tgacaagtct
     3901 ggtggttcta ctaatctgtc agatattatt gaaaaggaga ccggtaagca actggttatc
     3961 caggaatcca tcctcatgct cccagaggag gtggaagaag tcattgggaa caagccggaa
     4021 agcgatatac tcgtgcacac cgcctacgac gagagcaccg acgagaatgt catgcttctg
     4081 actagcgacg cccctgaata caagccttgg gctctggtca tacaggatag caacggtgag
     4141 aacaagatta agatgctctc tggtggttct cccaagaaga agaggaaagt ctaaccggtc
     4201 atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     4261 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4321 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4381 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4441 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     4501 cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4561 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4621 cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4681 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4741 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4801 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4861 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5341 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5701 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5761 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5821 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5881 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5941 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6001 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6061 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6121 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6181 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6241 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6301 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6361 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6421 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6481 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6541 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6601 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6661 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc
     6721 ccgatcccct agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     6781 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     6841 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     6901 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     6961 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     7021 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     7081 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     7141 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatc
//
