LOCUS       ND6-DdCBE-right_        7005 bp ds-DNA     circular SYN 01-JUL-2020
DEFINITION  COX8a MTS_3xFLAG_right  m.14459A TALE_2aa_DddE(149-178
            )_1xUGI)_ATP5B 3'UTR .
ACCESSION
VERSION     .
KEYWORDS    TALEN-CCR5L18-NtermN1.
SOURCE      synthetic DNA construct
  ORGANISM  human, from Guilinger paper 
            .
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK
            and Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     primer_bind     198..213
                     /Sequence="GCGCTGCTTCGCGATG"
                     /Hairpin_Tm="55.5"
                     /Tm="59.7"
                     /Self_Dimer_Tm="14.5"
                     /%GC="68.8"
                     /created_by="primer3"
                     /label="BM719"
     primer_bind     209..229
                     /Sequence="CGATGTACGGGCCAGATATAC"
                     /Hairpin_Tm="38.5"
                     /Tm="57.9"
                     /Self_Dimer_Tm="10.9"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="BM525"
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     687..706
                     /Sequence="CCCATTGACGTCAATGGGAG"
                     /Hairpin_Tm="71.5"
                     /Tm="58.6"
                     /Self_Dimer_Tm="59.4"
                     /%GC="55.0"
                     /created_by="primer3"
                     /label="BM720"
     primer_bind     769..789
                     /note="Human CMV immediate early promoter, forward primer"
                     /label="CMV-F"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     863..882
                     /note="T7 promoter, forward primer"
                     /label="T7"
     motif           898..903
                     /created_by="bmok"
                     /label="Kozak sequence"
     motif           904..906
                     /created_by="bmok"
                     /label="START"
     source          <907..>909
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /modified_by="hrees"
                     /label="From pHR_087"
     CDS             910..993
                     /created_by="bmok"
                     /label="Cox8a-MTS "
     source          <910..>993
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             994..1059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     source          <994..>1059
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1063..2679
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="ND6 Right TALE"
     CDS             1063..1476
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="mutant N-terminal domain"
     regulatory      1300..>1311
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="specific for any 5' residue"
     source          <1300..>1311
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(<1300..>1311,<1300..>1311)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     modified_base   1303..1305
                     /created_by="bmok"
                     /label="Q81R"
     modified_base   1306..1308
                     /created_by="bmok"
                     /label="W82G"
     modified_base   1309..1311
                     /created_by="bmok"
                     /label="S83A"
     misc_difference 1510..1515
                     /created_by="bmok"
                     /label="G"
     misc_difference 1612..1617
                     /created_by="bmok"
                     /label="A"
     misc_difference 1714..1719
                     /created_by="bmok"
                     /label="T"
     misc_difference 1816..1821
                     /created_by="bmok"
                     /label="G"
     misc_difference 1918..1923
                     /created_by="bmok"
                     /label="G"
     misc_difference 2020..2025
                     /created_by="bmok"
                     /label="C"
     misc_difference 2122..2127
                     /created_by="bmok"
                     /label="T"
     misc_difference 2224..2229
                     /created_by="bmok"
                     /label="A"
     misc_difference 2326..2331
                     /created_by="bmok"
                     /label="T"
     misc_difference 2428..2433
                     /created_by="bmok"
                     /label="T"
     misc_difference 2530..2535
                     /created_by="bmok"
                     /label="T"
     misc_difference 2557..2679
                     /created_by="bmok"
                     /label="C terminal canonical"
     source          <2680..>2685
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(<2680..>2685,<2680..>2685)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             <2686..2775
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="DddA(1398-1427)"
     source          <2686..>2775
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(<2686..>2775,<2686..>2775)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <2776..>3036
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <2776..>2787
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <2776..2787
                     /created_by="jonlevy"
                     /modified_by="jonlevy"
                     /label="Genscript 2xUGI"
     CDS             2788..3036
                     /created_by="kzhao"
                     /label="UGI"
     source          <2788..>3036
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <2788..>3036
                     /created_by="jonlevy"
                     /modified_by="jonlevy"
                     /label="Genscript 2xUGI"
     3'UTR           3043..3190
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="ATP5B 3'UTR"
     rep_origin      3236..3664
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     complement(3323..3342)
                     /note="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     3533..3554
                     /note="F1 origin, forward primer"
                     /label="F1ori-F"
     primer_bind     complement(3673..3693)
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /label="pBABE 3'"
     promoter        3678..4008
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     rep_origin      3859..3994
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     3921..3940
                     /note="SV40 promoter/origin, forward primer"
                     /label="SV40pro-F"
     promoter        4056..4103
                     /note="synthetic bacterial promoter"
                     /label="EM7 promoter"
     CDS             4122..4520
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
                     GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
                     GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
                     /label="BSD"
     polyA_signal    4678..4799
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(4715..4734)
                     /note="SV40 polyA, reverse primer"
                     /label="SV40pA-R"
     primer_bind     4769..4788
                     /note="SV40 polyA terminator, reverse primer"
                     /label="EBV-rev"
     primer_bind     complement(4848..4864)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     primer_bind     complement(4848..4864)
                     /note="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     complement(4861..4883)
                     /note="In lacZ gene"
                     /label="M13/pUC Reverse"
     protein_bind    4872..4888
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4896..4926)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4941..4962
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     misc_feature    complement(4973..4989)
                     /Sequence="GAAAGCGGGCAGTGAGC"
                     /Hairpin_Tm="44.7"
                     /Tm="58.7"
                     /Self_Dimer_Tm="None"
                     /%GC="64.7"
                     /created_by="primer3"
                     /label="BM524 "
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(5079..5096)
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     rep_origin      complement(5250..5838)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(5330..5349)
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     CDS             complement(6009..6869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     6632..6651
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     promoter        complement(6870..6974)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatggcct ccgttctgac cccgctgctg ctgcgtggcc tgaccggctc cgctcgtcgt
      961 ctgccagttc cgcgtgcgaa aatccattcc ctggactaca aagaccatga cggtgattat
     1021 aaagatcatg acatcgatta caaggatgac gatgacaaga tggacatcgc ggatctgcgt
     1081 accctgggtt acagccagca gcagcaggag aagatcaagc caaaggtgcg cagcaccgtg
     1141 gcccagcacc atgaagctct ggtgggtcac ggcttcaccc acgcgcacat cgtggctctg
     1201 agccagcacc cagccgcgct gggtaccgtg gccgtgaaat atcaggacat gattgctgcc
     1261 ctgccagagg ctacccatga agcgattgtg ggtgtgggca agagaggagc cggtgctcgt
     1321 gcgctggagg ccctgctgac cgtggccggt gaactgcgtg gcccgccgct gcagctggat
     1381 accggccagc tgctgaaaat cgcgaaacgt ggcggtgtga ccgctgtgga agctgtgcat
     1441 gcctggcgta atgctctgac cggtgccccg ctgaacctga ccccgcagca ggtggtggct
     1501 attgccagca acaacggcgg taaacaggct ctggaaaccg tgcagcgcct gctgccggtg
     1561 ctgtgccagg ctcatggtct gaccccggag caggtggtgg cgatcgctag caacatcggc
     1621 ggcaagcagg ctctggagac cgttcaggcc ctgttaccgg tgctgtgcca agcccatggt
     1681 ctgaccccgc agcaagttgt ggctattgcc agcaatggcg gtggcaaaca ggcgctggag
     1741 accgtgcagc gtctgttgcc ggtgctgtgc caagcccatg ggctgacccc gcagcaagtg
     1801 gttgccatcg ccagcaacaa cggtggcaag caggccctgg agaccgttca gcgcctgtta
     1861 ccggtgctgt gccaggccca tggcttaacc ccgcagcaag ttgtggccat cgctagcaac
     1921 aacggtggca aacaggctct ggagactgtt cagcgtctgc ttccggtgct gtgccaagcg
     1981 catggcctga ccccggaaca agttgttgct attgccagcc atgatggtgg caagcaggcg
     2041 ctggaaaccg ttcagcgcct gcttccggtg ctgtgccagg cgcatggatt aaccccgcag
     2101 caagtggtgg ccatcgccag caatggtggc ggtaaacagg ccctggaaac cgttcagcgt
     2161 ctgttaccgg tgctgtgcca ggcccatgga ttaaccccgg aacaagttgt ggctattgcg
     2221 tccaatatcg gcggcaagca ggcgctggaa actgtgcagg ctctgctccc ggtgctgtgc
     2281 caggcccatg ggttaacccc gcagcaggtt gttgccattg cgagcaacgg cggtggcaaa
     2341 caggctctgg agacggttca gcgcctgctc ccggtgctgt gccaggccca tggtttaacc
     2401 ccgcagcagg tggttgctat tgctagcaat ggcggcggca agcaggcgct ggaaacggtg
     2461 cagcgtctgc taccggtgct gtgccaggca catggcctta ccccgcagca agttgtggcc
     2521 attgctagca atggcggtgg ccgtccggcc ctggaaagca ttgtggcgca gctgagccgt
     2581 ccagacccgg ccctggcggc tctgaccaat gatcacctgg tggccctggc ctgcctgggt
     2641 ggccgtccgg ctctggatgc cgtgaagaaa ggtctgggcg gatccgccat tccagtgaag
     2701 cgcggcgcta ccggtgaaac caaagtgttt accggtaaca gcaacagccc gaagagcccg
     2761 accaaaggcg gttgctctgg cggctcaact aatctgagcg acatcattga gaaggagact
     2821 gggaaacagc tggtcattca ggagtccatc ctgatgctgc ctgaggaggt ggaggaagtg
     2881 atcggcaaca agccagagtc tgacatcctg gtgcacaccg cctacgacga gtccacagat
     2941 gagaatgtga tgctgctgac ctctgacgcc cccgagtata agccttgggc cctggtcatc
     3001 caggattcta acggcgagaa taagatcaag atgctgtgat aaggggtctt tgtcctctgt
     3061 actgtctctc tccttgcccc taacccaaaa agcttcattt ttctgtgtag gctgcacaag
     3121 agccttgatt gaagatatat tctttctgaa cagtatttaa ggtttccaat aaaatgtaca
     3181 cccctcagaa cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     3241 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3301 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3361 gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct
     3421 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3481 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3541 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3601 attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3661 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca
     3721 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3781 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     3841 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3901 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     3961 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     4021 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     4081 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag cctttgtctc
     4141 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     4201 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     4261 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     4321 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     4381 tgagcccctg cggacggtgt cgacaggtgc ttctcgatct gcatcctggg atcaaagcga
     4441 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     4501 gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     4561 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4621 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     4681 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4741 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4801 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     4861 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4921 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4981 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5041 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5101 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5161 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5221 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5281 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5341 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5401 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     5461 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5521 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5581 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5641 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     5701 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5761 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5821 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5881 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5941 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6001 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6061 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6121 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6181 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6241 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6301 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6361 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6421 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6481 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6541 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6601 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6661 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6721 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6781 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6841 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6901 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6961 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
