LOCUS       ND4-DdCBE-right_        7851 bp ds-DNA     circular SYN 01-JUL-2020
DEFINITION  COX8a MTS_3xFLAG_right  m.11922 TALE_2aa_DddE(41-148 )_1xUGI_ATP5B
            3'UTR .
ACCESSION
VERSION     .
KEYWORDS    TALEN-CCR5L18-NtermN1.
SOURCE      synthetic DNA construct
  ORGANISM  human, from Guilinger paper 
            .
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK
            and Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     primer_bind     198..213
                     /Sequence="GCGCTGCTTCGCGATG"
                     /Hairpin_Tm="55.5"
                     /Tm="59.7"
                     /Self_Dimer_Tm="14.5"
                     /%GC="68.8"
                     /created_by="primer3"
                     /label="BM719"
     primer_bind     209..229
                     /Sequence="CGATGTACGGGCCAGATATAC"
                     /Hairpin_Tm="38.5"
                     /Tm="57.9"
                     /Self_Dimer_Tm="10.9"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="BM525"
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     687..706
                     /Sequence="CCCATTGACGTCAATGGGAG"
                     /Hairpin_Tm="71.5"
                     /Tm="58.6"
                     /Self_Dimer_Tm="59.4"
                     /%GC="55.0"
                     /created_by="primer3"
                     /label="BM720"
     primer_bind     769..789
                     /note="Human CMV immediate early promoter, forward primer"
                     /label="CMV-F"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     863..882
                     /note="T7 promoter, forward primer"
                     /label="T7"
     motif           898..903
                     /created_by="bmok"
                     /label="Kozak sequence"
     motif           904..906
                     /created_by="bmok"
                     /label="START"
     misc_feature    907..991
                     /Original_Bases="TCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTC
                     GGCCCGGCGGCTCCCAGTGCCGCGCGCCAAGATCCATTCGT"
                     /label="TCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCGGCG
                     GCTCCCAGTGCCGCGCGCCAAGATCCATTCGT"
                     /note="Geneious type: Editing History Replacement"
     source          <907..>909
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /modified_by="hrees"
                     /label="From pHR_087"
     CDS             910..993
                     /created_by="bmok"
                     /label="Cox8a-MTS "
     source          <910..>993
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             994..1059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     source          <994..>1059
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1063..1476
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="N terminal canonical"
     CDS             1477..3168
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="ND4 Right TALE"
     source          <1477..>3168
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_difference 3169..3291
                     /created_by="bmok"
                     /label="C terminal canonical"
     source          <3292..>3297
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3298..3621
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="DddA(1290-1397)"
     source          <3298..>3621
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(<3298..>3621,<3298..>3621)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(<3298..>3621,<3298..>3621,<3298..>3621,<3298..>3621)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polylinker      3325..3366
                     /created_by="bmok"
                     /label="loop, might be involved in dsDNA binding "
     polylinker      3385..3396
                     /created_by="bmok"
                     /label="loop (SPLIT) "
     polylinker      3418..>3429
                     /created_by="bmok"
                     /label="loop "
     misc_feature    3469..3471
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="E1298A mutant catalytically dead "
     polylinker      3502..3510
                     /created_by="bmok"
                     /label="loop (SPLIT)"
     polylinker      3577..3588
                     /created_by="bmok"
                     /label="loop (split) "
     polylinker      3604..>3621
                     /created_by="bmok"
                     /label="loop "
     source          <3622..>3882
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <3622..>3633
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <3622..3633
                     /created_by="jonlevy"
                     /modified_by="jonlevy"
                     /label="Genscript 2xUGI"
     CDS             3634..3882
                     /created_by="kzhao"
                     /label="UGI"
     source          <3634..>3882
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          <3634..>3882
                     /created_by="jonlevy"
                     /modified_by="jonlevy"
                     /label="Genscript 2xUGI"
     overhang        3874..3886
                     /created_by="bmok"
                     /label="user junction "
     3'UTR           3889..4036
                     /created_by="bmok"
                     /modified_by="bmok"
                     /label="ATP5B 3'UTR"
     misc_feature    3890..4036
                     /Original_Bases="TTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGC
                     CAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTC
                     CCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCA
                     TTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAAT
                     AGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG"
                     /label="TTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCT
                     GTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCC
                     TTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCT
                     GGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCAT
                     GCTGGGGATGCGGTGGGCTCTATGG"
                     /note="Geneious type: Editing History Replacement"
     terminator      4014..4018
                     /created_by="bmok"
                     /label="AAUAA motif "
     rep_origin      4082..4510
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     complement(4169..4188)
                     /note="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     4379..4400
                     /note="F1 origin, forward primer"
                     /label="F1ori-F"
     primer_bind     complement(4519..4539)
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /label="pBABE 3'"
     promoter        4524..4854
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     rep_origin      4705..4840
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     4767..4786
                     /note="SV40 promoter/origin, forward primer"
                     /label="SV40pro-F"
     promoter        4902..4949
                     /note="synthetic bacterial promoter"
                     /label="EM7 promoter"
     CDS             4968..5366
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
                     GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
                     GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
                     /label="BSD"
     polyA_signal    5524..5645
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5561..5580)
                     /note="SV40 polyA, reverse primer"
                     /label="SV40pA-R"
     primer_bind     5615..5634
                     /note="SV40 polyA terminator, reverse primer"
                     /label="EBV-rev"
     primer_bind     complement(5694..5710)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     primer_bind     complement(5694..5710)
                     /note="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     complement(5707..5729)
                     /note="In lacZ gene"
                     /label="M13/pUC Reverse"
     protein_bind    5718..5734
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5742..5772)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5787..5808
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     misc_feature    complement(5819..5835)
                     /Sequence="GAAAGCGGGCAGTGAGC"
                     /Hairpin_Tm="44.7"
                     /Tm="58.7"
                     /Self_Dimer_Tm="None"
                     /%GC="64.7"
                     /created_by="primer3"
                     /label="BM524 "
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(5925..5942)
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     rep_origin      complement(6096..6684)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(6176..6195)
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     CDS             complement(6855..7715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     7478..7497
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     promoter        complement(7716..7820)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatggcct ccgttctgac cccgctgctg ctgcgtggcc tgaccggctc cgctcgtcgt
      961 ctgccagttc cgcgtgcgaa aatccattcc ctggactaca aagaccatga cggtgattat
     1021 aaagatcatg acatcgatta caaggatgac gatgacaaga tggacatcgc ggatctgcgt
     1081 accctgggtt acagccagca gcagcaggag aagatcaagc caaaggtgcg cagcaccgtg
     1141 gcccagcacc atgaagctct ggtgggtcac ggcttcaccc acgcgcacat cgtggctctg
     1201 agccagcacc cagccgcgct gggtaccgtg gccgtgaaat atcaggacat gattgctgcc
     1261 ctgccagagg ctacccatga agcgattgtg ggtgtgggca agcagtggag cggtgctcgt
     1321 gcgctggagg ccctgctgac cgtggccggt gaactgcgtg gcccgccgct gcagctggat
     1381 accggccagc tgctgaaaat cgcgaaacgt ggcggtgtga ccgctgtgga agctgtgcat
     1441 gcctggcgta atgctctgac cggtgccccg ctgaacctga ccccggaaca agtggttgcg
     1501 attgcgtccc atgatggtgg taaacaagcc ctggagaccg ttcaagctct gctgccagtg
     1561 ctgtgccagg ctcatggtct gaccccgcag caggtggtgg ctattgccag ccatgatggc
     1621 ggcaagcagg ctctggagac cgtgcagcgt ctgctgccgg tgctgtgcca agcccatggc
     1681 ctgaccccgc agcaagttgt ggctattgcc agcaacggcg gtggcaaaca ggcgctggaa
     1741 accgtgcagc gcctgttacc ggtgctgtgc caagcgcatg gtctgacccc ggagcaggtg
     1801 gtggccatcg ctagcaacaa cggtggcaag caggccctgg aaaccgtgca ggcgctgttg
     1861 ccggtgctgt gccaggccca tggcttaacc ccggaacaag tggtggccat tgcgagcaat
     1921 ggtggcggta aacaggctct ggaaaccgtg caggccctgc ttccggtgct gtgccaggcc
     1981 catgggctga ccccggaaca agttgtggct atcgccagca acatcggcgg caagcaggcg
     2041 ctggagaccg ttcaggctct gttaccggtg ctgtgccagg cgcatggcct gaccccggaa
     2101 caggtggtgg cgatcgctag caacattggc ggtaaacagg ccctggagac cgttcagcgc
     2161 ctgctcccgg tgctgtgcca ggcccatggt ctgaccccgg aacaggttgt tgctattgcc
     2221 agcaacaacg gcggcaagca ggccctggag accgtgcagg cgctgctacc ggtgctgtgc
     2281 caggcccatg gactgacccc ggagcaggtt gtggccatcg cgtccaatgg cggtggcaaa
     2341 caggctctgg agaccgttca gcgtctgtta ccggtgctgt gccaggcaca tggcctgacc
     2401 ccggagcaag ttgttgccat cgctagcaac attggtggca agcaggcgct ggaaaccgtt
     2461 cagcgcctgc taccggtgct gtgccaggct catggcttaa ccccggagca ggttgtcgcc
     2521 attgccagca acaatggtgg caaacaggct ctggaaactg tgcaggccct gctaccggtg
     2581 ctgtgccagg cccatgggtt aaccccggaa caggttgtgg ccattgcctc caataacggt
     2641 ggcaagcagg cgctggaaac ggtgcaggct ctgcttccgg tgctgtgcca ggctcatggg
     2701 ctgaccccgg agcaagtggt tgctattgcg tccaacattg gtggcaaaca ggccctggaa
     2761 accgttcagg cgctgctccc ggtgctgtgc caggcccatg ggctaacccc ggaacaggtg
     2821 gttgccattg cctccaacaa tggtggcaag caggccctgg aaacggttca gcgtctgctt
     2881 ccggtgctgt gccaggccca tgggcttacc ccgcagcaag ttgttgctat cgccagcaat
     2941 attggtggca aacaggctct ggaaacggtg cagcgcctgc taccggtgct gtgccaggct
     3001 catggtttaa ccccgcagca ggtggttgcg attgcctcca acaacggtgg caagcaggcg
     3061 ctggaaactg ttcagcgtct gctcccggtg ctgtgccagg ctcacggcct gaccccgcag
     3121 caagtggtgg ctatcgcctc caacggtggt ggtcgcccgg ctctggaaag cattgtggcg
     3181 cagctgagcc gtccagaccc ggccctggcg gctctgacca atgatcacct ggtggccctg
     3241 gcctgcctgg gtggccgtcc ggctctggat gccgtgaaga aaggtctggg cggatccggc
     3301 agctacgccc tgggtccgta tcagattagc gccccgcagc tgccagctta caatggtcag
     3361 accgtgggta ccttctacta tgtgaacgac gcgggcggtc tggagagcaa ggtgtttagc
     3421 agcggcggtc caaccccgta cccaaactat gccaatgccg gtcatgtgga gggtcagagc
     3481 gccctgttca tgcgtgataa cggcatcagc gagggtctgg tgttccacaa caacccggaa
     3541 ggcacctgcg gtttttgcgt gaacatgacc gagaccctgc tgccggaaaa cgcgaaaatg
     3601 accgtggtgc cgccggaagg ttctggcggc tcaactaatc tgagcgacat cattgagaag
     3661 gagactggga aacagctggt cattcaggag tccatcctga tgctgcctga ggaggtggag
     3721 gaagtgatcg gcaacaagcc agagtctgac atcctggtgc acaccgccta cgacgagtcc
     3781 acagatgaga atgtgatgct gctgacctct gacgcccccg agtataagcc ttgggccctg
     3841 gtcatccagg attctaacgg cgagaataag atcaagatgc tgtgataagg ggtctttgtc
     3901 ctctgtactg tctctctcct tgcccctaac ccaaaaagct tcatttttct gtgtaggctg
     3961 cacaagagcc ttgattgaag atatattctt tctgaacagt atttaaggtt tccaataaaa
     4021 tgtacacccc tcagaacttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     4081 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4141 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4201 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt
     4261 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4321 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4381 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4441 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4501 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4561 caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4621 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4681 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4741 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     4801 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4861 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     4921 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagcctt
     4981 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     5041 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     5101 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     5161 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     5221 gcatcttgag cccctgcgga cggtgtcgac aggtgcttct cgatctgcat cctgggatca
     5281 aagcgatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     5341 cctctggtta tgtgtgggag ggctaagcac ttcgtggccg aggagcagga ctgacacgtg
     5401 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     5461 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     5521 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5581 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5641 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     5701 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5761 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5821 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5881 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5941 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6001 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6061 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     6301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     6541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6781 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6841 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6901 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6961 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7021 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7081 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7141 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7201 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7261 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7321 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7381 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7441 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7501 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7561 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7621 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7681 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7741 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7801 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
