LOCUS       Exported                6456 bp ds-DNA     circular SYN 07-JUL-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDEST-hSTARR-luc-Pmyc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 7, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        34..257
                     /label=MYC promoter
     intron          292..521
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     CDS             567..2219
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     protein_bind    2252..2376
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2485..3165
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             3485..3790
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3831..3955)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    4010..4131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4550..5138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5338..6198)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    6303..6351
                     /note="synthetic polyadenylation signal"
     misc_feature    6365..6456
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcccct agggtctgga cggctgagga cccccgagct
       61 gtgctgctcg cggccgccac cgccgggccc cggccgtccc tggctcccct cctgcctcga
      121 gaagggcagg gcttctcaga ggcttggcgg gaaaaagaac ggagggaggg atcgcgctga
      181 gtataaaagc cggttttcgg ggctttatct aactcgctgt agtaattcca gcgagaggca
      241 gagggagcga gcgggcgatt aattcgctgt ctgcgagggc cagctgttgg ggtgagtact
      301 ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa aacgaggagg
      361 atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc atctggtcag
      421 aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc tggccataca
      481 cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca
      541 actgcaggtc gcctgcaggc ttaagcatgg aagatgccaa aaacattaag aagggcccag
      601 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc
      661 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta
      721 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc
      781 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg
      841 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc
      901 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag
      961 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca
     1021 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt
     1081 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca
     1141 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc
     1201 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca
     1261 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca
     1321 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc
     1381 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat
     1441 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga
     1501 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc
     1561 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca
     1621 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta
     1681 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg
     1741 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca
     1801 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact
     1861 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag
     1921 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc
     1981 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa
     2041 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc
     2101 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg
     2161 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat
     2221 ctagagcatg caccggtgat agcttgatca aacaagtttg tacaaaaaag ctgaacgaga
     2281 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     2341 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
     2401 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     2461 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     2521 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     2581 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     2641 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     2701 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     2761 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     2821 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     2881 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     2941 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     3001 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     3061 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     3121 gatgagtggc aggcggggcg taatctagag gatccggctt actaaaagcc agataacagt
     3181 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     3241 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     3301 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     3361 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     3421 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     3481 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     3541 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     3601 acgtctgctg tcagataaag tcccccgtga actttacccg gtggtgcata tcggggatga
     3661 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     3721 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     3781 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     3841 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     3901 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttcgat
     3961 ctagaatgga tcgtcgacga attcggccgg ccgcttcgag cagacatgat aagatacatt
     4021 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     4081 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     4141 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     4201 taaaacctct acaaatgtgg taaaatcgat aaggatccga ccgatgccct tgagagcctt
     4261 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     4321 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcagcgctct tccgcttcct
     4381 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4441 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4981 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5281 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta
     5341 ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt
     5401 ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag
     5461 cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca
     5521 gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc
     5581 tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt
     5641 tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa
     5701 ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt
     5761 cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat
     5821 ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt
     5881 gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc
     5941 ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat
     6001 catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag
     6061 ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt
     6121 ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg
     6181 aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta
     6241 ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc
     6301 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     6361 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     6421 tccccagtgc aagtgcaggt gccagaacat ttctct
//
