LOCUS       Exported                2349 bp ds-DNA     circular SYN 02-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Linyi Gao
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 2, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      129..164
                     /label=T7 Terminator
     CDS             complement(396..1055)
                     /codon_start=1
                     /label=CAT/CamR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1056..1158)
                     /label=cat Promoter
     misc_feature    1417..2329
                     /label=p15A_origin
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaagctt ctgcagaatt
      121 cgcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      181 aacctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa
      241 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga
      301 atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca
      361 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
      421 caatactgtt gtaattcatt taacattctg ccgacatgga agccatcaca gacggcatga
      481 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccata
      541 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
      601 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
      661 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
      721 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
      781 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcatcgc catacggaac
      841 tctggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
      901 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
      961 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1021 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     1081 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1141 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
     1201 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
     1261 tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt
     1321 ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg
     1381 ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct
     1441 tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg
     1501 ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg
     1561 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag
     1621 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc
     1681 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     1741 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt
     1801 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     1861 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     1921 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1981 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
     2041 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
     2101 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
     2161 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
     2221 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt
     2281 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
     2341 tagtcatgc
//
