LOCUS       Exported               16037 bp ds-DNA     circular SYN 02-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16037)
  AUTHORS   Linyi Gao
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 2, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16037
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          106..13808
                     /organism="Escherichia coli"
                     /strain="NCTC9078"
                     /mol_type="genomic DNA"
                     /serovar="not available: to be reported later"
                     /label=contig: ERS523585SCcontig000005
                     /note="contig: ERS523585SCcontig000005"
                     /db_xref="taxon:562"
     CDS             409..1014
                     /codon_start=1
                     /locus_tag="DX464_RS21520"
                     /old_locus_tag="NCTC9078_04397"
                     /product="DUF1819 family protein"
                     /label=brxA
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_085962535.1"
                     /translation="MIKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGH
                     NILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALMLHSPVVKDF
                     LAEVVNDLRRQFKEKLPGNSWNEFVNSQVRLHPVLASYSDSSIAKMGNNLVKALAEAGY
                     VDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR"
     CDS             1011..1613
                     /codon_start=1
                     /locus_tag="DX464_RS21515"
                     /old_locus_tag="NCTC9078_04396"
                     /product="DUF1788 domain-containing protein"
                     /label=brxB
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_000566901.1"
                     /translation="MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCEL
                     QVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQLAG
                     PLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMFYPG
                     TYSGYNLSPLTDTGSQNYYRAFRLVPDTGPAATLNPQ"
     gene            1011..1613
                     /locus_tag="DX464_RS21515"
                     /old_locus_tag="NCTC9078_04396"
                     /label=DX464_RS21515
     CDS             1625..5266
                     /codon_start=1
                     /gene="brxC"
                     /locus_tag="DX464_RS21510"
                     /old_locus_tag="NCTC9078_04395"
                     /product="BREX system P-loop protein BrxC"
                     /label=brxC
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_001019648.1"
                     /translation="MNIEQIFEKPLKRNINGVVKAEQTDDASAYIELDEYVITRELENH
                     LRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTERN
                     AYSFFEDKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNERIG
                     YCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALSQATQ
                     QSLESSRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNTQMMLK
                     LQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQLSSSNT
                     SEVIQKRLLVKTDEAKAALAKVWQEKADILRNQLAFDTTTTTALRPFTSEEEFVDNYPF
                     VPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSLVPFWRFYA
                     AIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNLVTLSIDRID
                     ADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDVDFSAINKKLA
                     SIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTPKDPTYSFYNSD
                     ATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPEQATLLSEKAREN
                     SNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVIENTFGKLKMLRPF
                     NGDISREIHALLTVENDTELDLGNLEESNPDAMREVETWISMNIEYNKPVYLRDILNHF
                     ARRPYGWPEDEVKLLVARLACKGKFSFSQQNNNVERKQAWELFNNSRRHSELRLHKVRR
                     HDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQELNVFRAKAEGGNNPGK
                     NEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDREDLVDFYRKQFATWQKLGA
                     ALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNRITPLIEQVQNVNHQLVEQ
                     HRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKARKRAEVSQSIPEILAEQQE
                     TKALQMDADKKINLWIDELRKKQEAQLRAANEAKRAADSEQTYVVVEKTVIQPVPKKTH
                     LVNVASEMRNATGGEVLETTEQVEKALDTLRTTLLAVIKAGDRIRLQ"
     gene            1625..5266
                     /gene="brxC"
                     /locus_tag="DX464_RS21510"
                     /old_locus_tag="NCTC9078_04395"
                     /label=brxC
     CDS             5312..8917
                     /codon_start=1
                     /gene="pglX"
                     /locus_tag="DX464_RS21505"
                     /old_locus_tag="NCTC9078_04394"
                     /product="BREX-1 system 
                     adenine-specificDNA-methyltransferase PglX"
                     /label=pglX
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_021524842.1"
                     /translation="MNTNNIKKYAPQARNDFRDAVIQKLTTLGIAADKKGNLQIAEAET
                     IGETVRYGQFDYPLSTLPRRERLVKRAREQGFEVLVEHCAYTWFNRLCAIRYMELHGYL
                     EHGFRMLSHPETPTAFEVLDHVPEVAEALLPENKAQLVEMKLSGNQDEALYRELLLGQC
                     HALHHAMPFLFEAVDDEAELLLPDNLTRTDSILRGLVDDIPEEDWEQVEVIGWLYQFYI
                     SEKKDAVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRQWLQTYPDSPLKDKMEYYI
                     EPAEQTPEVQAQLAAITPASIEPESIKVLDPACGSGHILIEAYNVLKNIYEERGYRGRD
                     IPQLILENNIFGLDIDDRAAQLSGFALLMMARQDDRRIFTRDVRLNIVSLQESLHLDIA
                     KLWQQLNFHQQVQTGSMGDMFAENNALTQTDSAEYQLLMRTLKRFVNAKTLGSLIQVPQ
                     EEEAELKVFLDALYRLEQEGDFQQKTAAKAFIPFIQQAWILAQRYDAVVANPPYMGGNY
                     METELKNFVSSYYPQGKADLYSSFMVRLLLQLKDNRTLSLMTPFTWMNLSSFEELRKII
                     LTNFSIQSLVQPEYHSFFESAYVPICAFSISNTPLSWNAKFFDLSDFYGEKNQAPNFQY
                     AIKNDNKCHWKYNRITTDFLCTPGYIIAYSLPDSALSCFKTSKKLHDVCNLKQGLITGD
                     NERYLRFWHEISYNSFSLNEKRKKTKWFPYQKGGAYRKWYGNNDYVVDWENDGYSIKNF
                     YNDKGKLRSRPQNIQFYCKEGLTWTSLTISSLSMRYVPNGYIFDAKGPMCFPKSSLDIW
                     NILGYANSKVIDIFLKQLAPTMDYSQGPVGNVPFKFNDGDLNEIIKELVNIHKRDWDEN
                     ETSFEFKRDMLVHFSRDINTIKGSFTLRQGENKKAINRTKFLEEMNNSFFINCFNLTDI
                     LSPEIELNKITLTHATIEIDIQKIISYAIGCQMGRYSLDREGLVYAHEGNNGFADLVAE
                     GAYKSFPADSDGILPLMDEEWFDDDVTSRVKEFIRTVWGEEYLRENLDFIAEVLKPKKG
                     ESALETIRRYLSTQFWKDHLKMYKKRPIYWLFSSGKEKAFECLVYLHRYNDATLSRMRT
                     EYVVPLLARYQANIDRLNDQLDEASGGESTRLKRERDSLIKKFSELRSYDDRLRHYADM
                     RISIDLDDGVKVNYGKFGDLLADVKAITGNAPEVI"
     gene            5312..8917
                     /gene="pglX"
                     /locus_tag="DX464_RS21505"
                     /old_locus_tag="NCTC9078_04394"
                     /label=pglX
     CDS             9094..11691
                     /codon_start=1
                     /gene="pglZ"
                     /locus_tag="DX464_RS21500"
                     /old_locus_tag="NCTC9078_04393"
                     /product="BREX-1 system phosphatase PglZ type A"
                     /label=pglZ
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_001180895.1"
                     /translation="MQNQDFIAGLKAKFAEHRIVFWHDPDKRFIEELEQLKLESVTLIN
                     MTHESQLAVKKRIEIDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHADFAAITLNT
                     LGIPQLGLREHIQRRKAFFSTKRTQALKNLATEQEDEASLDKKMIAVIAGAKTAKTEDI
                     LFNLITQYVNQQIEDDSELENTQAMLKRHGLDSVLWEMLNHEMGYQAEEPSLENLLLKL
                     FCTDLSAQADPQQRAWLEKNVLLTPSGRASALAFMVTWRADRRYKEAYDYCAQQMQAAL
                     HPEDHYRLSSPYDLHECETTLSIEQTIIHALVTQLLEESTTLDREAFKKLLSERQSKYW
                     CQTQPEYYAIYDALRQAERLLNLRNRHIDGFHYQDSATFWKAYCEELFRFDQAYRLFNE
                     YALLVHSKGAMILKSLDDYIEALYSNWYLAELSRNWNEVLEAENRMQAWQIPGVPRQQN
                     FFNEVVKPQFQNPQIKRVFVIISDALRYEVAEELGNQINTEKRFTAELRSQLGVLPSYT
                     QLGMAALLPHEQLCYQPGNGDIVYADGLSTSGIPNRDTILKNYKGMAIKSKDLLELKNQ
                     EGRDLIRDYEVVYIWHNTIDATGDTASTEDKTFEACRTAVAELKDLVTKVINRLHGTRI
                     FVTADHGFLFQQQALSVQDKTTLQIKPENTIKNHKRFIIGHQLPADDFCWKGKVADTAG
                     VSDNSEFLIPKGIQRFHFSGGARFVHGGTMLQEVCVPVLQIKALQKTAAEKQPQRRPVD
                     IVAYHPMIKLVNNIDKVSLLQTHPVGELYEPRILNIYIVDNANNVVSGKERISFDSDNN
                     TMEKRVREVTLKLIGANFNRRNEYWLILEDAQTETGYQKYPVIIDLAFQDDFF"
     gene            9094..11691
                     /gene="pglZ"
                     /locus_tag="DX464_RS21500"
                     /old_locus_tag="NCTC9078_04393"
                     /label=pglZ
     CDS             11702..13786
                     /codon_start=1
                     /gene="brxL"
                     /locus_tag="DX464_RS21495"
                     /old_locus_tag="NCTC9078_04392"
                     /product="protease Lon-related BREX system protein BrxL"
                     /label=brxL
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_001193074.1"
                     /translation="MQTHHDLPVSGVSAGEIASEGYDLDALLNQHFAGRVVRKDLTKQL
                     KEGANVPVYVLEYLLGMYCASDDDDVVEQGLQNVKRILADNYVRPDEAEKVKSLIRERG
                     SYKIIDKVSVKLNQKKDVYEAQLSNLGIKDALVPSQMVKDNEKLLTGGIWCMITVNYFF
                     EEGQKTSPFSLMTLKPIQMPNMDMEEVFDARKHFNRDQWIDVLLRSVGMEPANIEQRTK
                     WHLITRMIPFVENNYNVCELGPRGTGKSHVYKECSPNSLLVSGGQTTVANLFYNMASRQ
                     IGLVGMWDVVAFDEVAGITFKDKDGVQIMKDYMASGSFSRGRDSIEGKASMVFVGNINQ
                     SVETLVKTSHLLAPFPTAMIDTAFFDRFHAYIPGWEIPKMRPEFFTNRYGLITDYLAEY
                     MREMRKRSFSDAIDKFFKLGNNLNQRDVIAVRRTVSGLLKLMHPDGAYSKEDVRVCLTY
                     AMEVRRRVKEQLKKLGGLEFFDVNFSYIDNETLEEFFVSVPEQGGSELIPAGMPKPGVV
                     HLVTQAESGMTGLYRFETQMTAGNGKHSVSGLGSNTSAKEAIRVGFDYFKGNLNRVSAA
                     AKFSDHEYHLHVVELHNTGPSTATSLAALIALCSILLAKPVQEQMVVLGSMTLGGVINP
                     VQDLAASLQLAFDSGAKRVLLPMSSAMDIPTVPAELFTKFQVSFYSDPVDAVYKALGVN
                     "
     gene            11702..13786
                     /gene="brxL"
                     /locus_tag="DX464_RS21495"
                     /old_locus_tag="NCTC9078_04392"
                     /label=brxL
     terminator      13817..13852
                     /label=T7 Terminator
     CDS             complement(14084..14743)
                     /codon_start=1
                     /label=CAT/CamR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(14744..14846)
                     /label=cat Promoter
     misc_feature    15105..16017
                     /label=p15A_origin
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaacagc accacgttca
      121 tcttcctttt ttaactgatt ttacagagac tttaatacag ttaaaatttt atttcctgag
      181 ctgtaatcga ttaagttgat gcatttaatg ggaatgatat agggtcattt ccagtctcac
      241 ttatagaaat ggctaaagca tgactctcgc caaaaccgtt tatgtgttgt acataacgcg
      301 atcatccctc tcacaaattg ccttttctca tggcatctcg cccggtcccc cattacaatc
      361 actttttgtt ttttgcgagc tgcattccag tcttcagagg gtttttcgat gattaaaaat
      421 gacaaggcat ggataggaga cttgctgggc ggaccgctca tgagcaggga aagccgcgtc
      481 attgccgaac tgttgctaac cgatcccgat gaacagacat ggcaagagca aattgttggc
      541 cacaacattt tacaagcctc ttctcctaac accgcaaaac gttacgcggc aacaatcagg
      601 cttcgcctga acacgctgga taaaagcgcg tggacattga ttgccgaagg tagtgaacgg
      661 gaacgccaac aacttctgtt tgtggctctg atgctacatt cgccggtagt taaggatttt
      721 ctggctgaag tggtgaacga tctgcgcagg cagttcaagg aaaagttgcc tggcaatagc
      781 tggaacgaat ttgtgaatag ccaggttcgc ctacatccgg tactcgccag ctactcagat
      841 tcatctattg caaaaatggg aaacaatctg gtgaaggcgc ttgctgaagc gggttatgtg
      901 gatacgcccc gcagacgtaa cctgcaggca gtttaccttt taccggaaac tcaggcagtg
      961 ttacagcgcc tgggacaaca ggacttgata tctattctgg agggaaaacg gtgatagatc
     1021 ccgttcttga atatcgcctg tctcaaatcc agagtcgcat taacgaagat cgcttcctca
     1081 aaaataacgg ctccggaaat gaaattggtt tttggatctt tgattatccc gcgcagtgcg
     1141 aactgcaggt acgggagcat ttgaaatatc tgctccggca tctggaaaag gaccataaat
     1201 ttgcctgtct gaatgtcttc caaatcatca tcgatatgct caatgaacgc ggccttttcg
     1261 agcgcgtctg ccagcaggaa gtcaaagtgg gtactgagac gctgaaaaag cagcttgctg
     1321 gtccgttaaa tcagaaaaag atcgctgatt ttatagcgaa aaaagtcgat ctggctgccc
     1381 aggattttgt cattcttacc ggcatgggca acgcctggcc attagtacgc ggtcatgaac
     1441 tgatgagtgc cttgcaggat gtcatggggt tcaccccact gctgatgttt tatcctggca
     1501 cctacagcgg gtacaacctt tccccgctca cagacaccgg ttcacaaaat tattatcgcg
     1561 ctttcagact ggtaccagat acgggacccg cagcaacatt gaatcctcaa tgaagagcat
     1621 aacaatgaat attgaacaga tttttgaaaa acctctaaaa cgaaatataa acggggtagt
     1681 caaagcagag caaaccgatg atgccagcgc gtacatcgag ttagatgaat atgtcatcac
     1741 ccgcgaactg gaaaaccatc ttcgccattt cttcgaatcc tatgttcctg ccactggccc
     1801 ggaacggatc cgtatggaaa acaagatcgg cgtatgggtt tcaggcttct tcggttcagg
     1861 taaatcgcac tttattaaga ttctttctta tcttttatct aaccgcaaag ttacacataa
     1921 cggtacggaa cgtaatgctt actccttctt tgaagataaa atcaaagatg cattattcct
     1981 tgccgatatt aacaaagcgg tgcattaccc gactgaagtc attctgttca atattgattc
     2041 gcgtgccaac gtagatgaca aagaagatgc cattcttaaa gtcttcctga aagttttcaa
     2101 cgaacgcatt ggatactgcg ctgattttcc gcatattgcc catcttgagc gcgagctgga
     2161 taaacgcggt cagtatgaaa cctttaaagc cgcgtttgcc gatatcaatg gctcgcgctg
     2221 ggaagacgag cgcgacgctt actacttcat cagcgatgac atggcacaag cattaagcca
     2281 ggccacgcag cagagtcttg aatcctcccg ccaatgggtg gaacaactcg acaaaaactt
     2341 cccgctggat atcaataatt tttgccagtg ggtaaaagag tggctggatg acaatggtaa
     2401 gaacatcctc tttatggtgg atgaagtcgg tcagttcatt ggcaaaaata cgcaaatgat
     2461 gctgaagctg cagactatta ctgaaaacct tggggtaatt tgcggtggcc gcgcatgggt
     2521 tatcgtgact tcgcaggccg atatcaacgc ggcaatcggt ggtatgagca gtcgcgacgg
     2581 acaggacttc tccaagatcc aggggcgctt ctctacacgc ctgcaacttt ccagctctaa
     2641 cacatcagaa gttatccaga aacgtttgtt ggtaaagact gacgaagcaa aagcggcact
     2701 ggcaaaagtg tggcaagaga aagccgatat cctgcgtaac cagctggctt ttgacactac
     2761 aacaactact gcactacgtc cttttaccag cgaagaagag ttcgttgaca actacccgtt
     2821 tgtcccgtgg cactatcaga ttctgcaaaa agtgtttgaa tctattcgga cgaaaggtgc
     2881 agcgggtaaa caattggcca tgggtgagcg ttctcagctg gaggcattcc agacggcggc
     2941 gcagcaaatc tcagcgcaag ggctggattc tctggtgcct ttctggcgct tctatgccgc
     3001 cattgagagc ttcctggaac ctgccgttag ccgcaccatc actcaggctt gccagaatgg
     3061 cattcttgat gagttcgatg gcaacctgct taaaacgctg ttcctgatcc gctatgtgga
     3121 aacgctgaaa agcaccctgg ataacctggt cacattgtct atcgatagga tcgatgccga
     3181 taaagttgag ttgcgccgcc gggtcgaaaa aagtctcaac acgcttgaac gcctgatgct
     3241 cattgcgcgc gttgaagata aatatgtgtt cctgaccaac gaagagaaag agatcgaaaa
     3301 cgagatccgt aacgttgatg tcgatttctc tgcgatcaac aaaaaactgg catcgatcat
     3361 ctttgatgac attctgaaaa gccgtaaata tcgttatccg gctaacaagc aagactttga
     3421 tatcagccgc ttcctgaacg ggcatccatt agacggcgca gtgcttaacg atctggtggt
     3481 gaagatcctg acccctaaag atccgactta ttcgttctat aacagcgatg cgacctgtcg
     3541 cccttatacg tcagaaggcg acggctgtat tttgattcgt ctgcccgaag agggccgtac
     3601 ctggagcgat attgatttag tcgtccagac tgaaaagttc ctcaaagata acgccgggca
     3661 acgtccggaa caggcaaccc tgctctcaga aaaagcgcgt gaaaacagca accgggaaaa
     3721 attactccgt gttcagttgg aatcactact tgcagaagca gacgtctggg cgattggcga
     3781 acgcttaccg aaaaaatcct ccacgccatc gaacattgtc gatgaagcct gccgttacgt
     3841 gattgaaaac accttcggca agctgaagat gctgcggcct tttaacggtg acatctcccg
     3901 tgaaattcat gcattactga cggttgagaa cgacaccgaa ctggatctcg gtaacctcga
     3961 agagtccaac cccgacgcca tgcgcgaggt agaaacctgg atcagcatga atatcgaata
     4021 caataaacct gtgtatttac gcgatattct gaaccatttt gcgcgtcgcc cttatggctg
     4081 gcccgaagac gaagtgaaac tgctagtagc ccgtctggcc tgcaaaggta aattcagctt
     4141 cagccagcaa aacaacaacg tcgagcgaaa acaggcgtgg gagttattta ataacagccg
     4201 ccgccatagc gaattgcgtc tgcataaagt tcgccgtcat gatgaagcgc aggtgcgtaa
     4261 agccgcgcaa accatggctg acatcgctca gcagccgttt aacgaacggg aagagccggc
     4321 gctggttgaa catattcgtc aggtatttga agagtggaag caagagctga acgtattccg
     4381 cgccaaggca gagggcggaa acaatccggg gaaaaacgag attgaatccg gtctgcgcct
     4441 gcttaatgcc attcttaatg agaaagaaga ttttgccctg atcgaaaaag tctcatcgct
     4501 gaaagatgaa cttctggatt tcagcgaaga ccgtgaagat ttggtcgact tctaccgtaa
     4561 gcaattcgcc acctggcaaa aactgggtgc tgcgctgaat ggcagcttta aatctaaccg
     4621 cagcgcgctg gaaaaagacg ccgcagcggt taaagcgctg ggcgagctgg aaagcatctg
     4681 gcaaatgccg gaaccttata agcatctcaa tcgcatcacg ccgttgattg aacaggtcca
     4741 gaacgtcaac catcagttag tcgaacagca tcgccagcac gccctcgaac gcattgacgc
     4801 ccgcattgag gaaagccgtc aacgcttgct ggaagcgcac gccacgtcgg agctgcaaaa
     4861 cagcgttctg ctgccgatgc aaaaagccag aaaacgcgct gaagtcagcc agtcgattcc
     4921 ggaaattttg gcggaacagc aagagacaaa agcgctgcaa atggatgcag ataaaaagat
     4981 taacctgtgg atcgacgagc tgcgtaaaaa gcaagaagca caactccggg cagcaaatga
     5041 agctaaacgc gctgccgact cagaacagac ttatgttgtg gtggaaaaaa ccgttatcca
     5101 accggtaccg aaaaaaacgc atctggtgaa tgtcgccagt gagatgcgta atgccaccgg
     5161 tggtgaagtt ctggaaacga ccgaacaggt ggaaaaggcg ctcgacacgt tacgcacaac
     5221 gctgctggcc gtcattaaag caggcgatcg cattcgcctt cagtaactcc catttcaggg
     5281 cagcactctg ctgccctttg caggattttc tatgaatacc aataacatta aaaaatatgc
     5341 cccacaggcc cgtaacgact tccgcgatgc ggtgatccag aagctaacga cgcttgggat
     5401 cgctgcagat aaaaaaggca atttgcagat tgccgaggcc gaaaccattg gcgagaccgt
     5461 gcgttacggt cagtttgatt acccgttatc gacccttccc cgccgcgaac ggctggtaaa
     5521 acgcgcccgt gagcagggtt ttgaggtgct ggttgagcac tgcgcctaca cctggtttaa
     5581 ccgcttatgt gcaattcgct atatggagct acacggttat cttgagcacg gcttccgtat
     5641 gttgtcccac ccggagacgc cgaccgcgtt tgaggtgctg gatcatgtgc cggaagtggc
     5701 agaagccctg ctgccggaaa ataaggcgca gctggttgaa atgaagcttt ccggtaatca
     5761 ggacgaagcc ctgtaccgcg aactgctgct ggggcagtgc cacgccctgc accacgcgat
     5821 gccgttcctg tttgaagcgg tagatgacga agcggaactg ctgttgccgg ataacctgac
     5881 ccgtaccgac tctattctgc gtgggctggt tgatgatatt ccggaagaag actgggagca
     5941 ggtagaggtt atcggctggc tgtatcagtt ctatatttcg gaaaagaaag atgccgtgat
     6001 tggcaaagtg gtgaagagcg aagatattcc tgccgccacc cagctgttta cgccaaactg
     6061 gattgtgcag tatctggtac aaaactccgt tggccgccag tggttgcaga cctacccgga
     6121 ctcgccgctg aaagacaaaa tggagtacta catcgagcct gcggaacaaa cgccggaagt
     6181 gcaggcgcag ctggcggcga ttaccccagc cagcattgaa cccgaaagta ttaaagtgct
     6241 cgacccagcc tgcggctccg gtcatatttt gattgaagcc tataatgtgc tgaaaaatat
     6301 ctacgaagag cgtggttatc gcgggcgtga tattccacaa ctgattctgg aaaataatat
     6361 ttttggtctt gatatcgacg accgcgcggc acagctttcc ggctttgcat tattaatgat
     6421 ggcgcgtcag gatgaccgca gaatatttac ccgcgatgta cgtctgaata ttgtctcttt
     6481 gcaggaaagc ctgcatctgg atatcgccaa actctggcag caactgaatt tccaccagca
     6541 ggtacaaacc ggcagtatgg gggatatgtt tgctgaaaat aacgcgttaa cccaaactga
     6601 cagcgcagaa tatcagctgc tgatgcgcac gctgaaacgc tttgtgaatg caaaaacgct
     6661 gggctcactg attcaggtgc cgcaggaaga agaagcggaa ctgaaggtat tcctggacgc
     6721 gttgtatcgc ctggaacagg aaggcgattt ccagcagaag acggcggcaa aagcgtttat
     6781 tccgtttatt cagcaggcgt ggattttagc gcagcgatat gatgcggtag tggcgaatcc
     6841 gccgtatatg gggggtaatt atatggagac agaacttaag aatttcgtct cttcttacta
     6901 ccctcaagga aaggcggatc tttattcttc atttatggtc agattacttt tacaattaaa
     6961 agataatcgc actttaagcc taatgacccc ctttacttgg atgaatttat catcatttga
     7021 agagctccga aaaattatac ttacaaattt cagcattcag tcattagtac agcctgaata
     7081 tcattcattt tttgagtcag cttatgtccc aatttgtgct tttagcattt caaatacccc
     7141 attaagctgg aatgcaaaat tttttgattt atcagatttt tatggagaaa aaaatcaagc
     7201 tccaaatttt cagtatgcaa ttaaaaatga caataaatgt cattggaaat ataacagaat
     7261 caccacggac tttctatgta ctcccggata tatcattgct tactctctgc ctgattctgc
     7321 gttatcttgc ttcaaaacat ccaaaaaact tcatgatgtt tgcaatctaa aacaaggatt
     7381 aattactggt gataatgaaa gatacctaag attctggcat gaaatcagct ataactcttt
     7441 cagtctcaat gaaaaaagaa aaaaaacaaa atggttccca tatcaaaaag gtggtgcata
     7501 ccgtaaatgg tatggtaata atgattatgt tgttgactgg gagaatgatg gttattccat
     7561 taaaaacttt tataatgaca aaggtaaatt acgctcacgc cctcaaaaca tacaatttta
     7621 ttgtaaagag ggtttaacat ggacaagttt aactatttcg tcactatcga tgagatatgt
     7681 accaaatgga tatatttttg atgcaaaagg acctatgtgt tttccgaaat cctctttgga
     7741 tatctggaat attcttggct atgcgaatag caaagtaata gatatatttc tcaaacaatt
     7801 agcgcccacc atggattatt ctcaagggcc tgttggaaat gtcccattca aatttaacga
     7861 tggtgatttg aacgagataa taaaagaact cgtaaacatt cacaaacgtg actgggatga
     7921 aaatgaaaca tcttttgagt ttaagagaga tatgttggtt catttttcaa gagatattaa
     7981 cactattaag ggtagtttta cactaaggca aggggaaaat aaaaaagcga ttaacagaac
     8041 aaaattttta gaagaaatga ataactcttt ctttataaat tgctttaatc taactgatat
     8101 tttatctcca gaaattgaac taaacaaaat cacgttaacg catgcaacta ttgaaattga
     8161 tattcaaaaa ataatttcat atgcaatagg ctgccaaatg ggacgttact cccttgatcg
     8221 cgaaggtctg gtatacgctc atgaaggcaa taatggcttc gccgatcttg tcgccgaagg
     8281 tgcttataaa agcttcccgg ctgatagtga cggcattctg ccgctaatgg atgaagagtg
     8341 gtttgacgat gacgtcacct ctcgcgtcaa ggagtttatc cgcaccgttt ggggcgaaga
     8401 atatttgcgc gaaaacctcg attttatagc cgaagttctc aagcccaaaa aaggcgaatc
     8461 tgcgctggag accattcgtc gctatctttc cacccagttc tggaaagatc atctgaaaat
     8521 gtataaaaag cgtccaatct actggctatt cagctccggt aaagagaaag cgtttgagtg
     8581 cttggtgtat ctgcatcgct ataacgatgc cacgctgtcg agaatgcgta ccgaatatgt
     8641 ggtgccgctg ctggcgcgtt atcaggccaa tattgatcgc ctgaacgatc aacttgatga
     8701 ggcttctggc ggtgaatcca cacgtctgaa acgcgaacgc gacagcctga tcaaaaaatt
     8761 cagcgaactg cgcagctatg acgatcgcct gcgtcactat gctgatatga gaatcagtat
     8821 tgatctcgac gatggcgtta aggttaacta cggcaagttt ggcgatctgc tggcagatgt
     8881 caaagccatc accggcaatg ccccagaggt gatctaaacc agacggcacg ttctcctgtt
     8941 gccgggttct gcccggtggc aaataccacc gggaaacgcg ccgctgctga catttctcca
     9001 cctcacttca tgataaaatg cgccaccgtg tcaaaatctc cttttcgcgt tttggcgctt
     9061 tcttattcat cgtaacaaca tgggattgtg aacttgcaaa atcaggactt tattgctggc
     9121 cttaaagcta aatttgccga acatcgcatc gttttctggc acgatcccga taaacgtttt
     9181 attgaggaac tggaacagct caagcttgaa agcgtcacgc taatcaacat gacccacgag
     9241 tcacagctgg cggtaaaaaa acgcatcgag attgatgagc cagaacagca gttcctgctg
     9301 tggttccccc atgatgcgcc gcctcatgaa caagactggc tgctggatat ccgcctttac
     9361 agcagcgaat tccatgccga ttttgccgcc atcaccctga acacgctggg cattccccag
     9421 cttggcctgc gcgagcatat tcagcgacgc aaggccttct tcagcactaa acgcacgcag
     9481 gcgctgaaaa atctggcgac agaacaggaa gatgaagcct cgctggataa gaaaatgatt
     9541 gcggtgatcg ctggcgcaaa gaccgcgaaa accgaagaca ttttgttcaa cctgattacc
     9601 cagtacgtta accaacaaat agaagacgac agcgaactgg aaaacacgca ggcgatgctg
     9661 aaacgccacg gtctggactc ggtattgtgg gaaatgctca accacgaaat gggctaccag
     9721 gcagaggagc catcgctgga aaacctgctc ctgaaactgt tttgtaccga tctctctgcc
     9781 caggccgacc cacagcagcg cgcctggctg gaaaaaaatg tcctgctgac gccatccggc
     9841 agagcatctg ccctggcatt tatggtgacc tggcgtgccg atcgtcgcta taaagaggct
     9901 tatgactact gcgctcagca aatgcaggcc gccctgcacc cggaagatca ttaccgactc
     9961 agctcgccgt atgatttgca cgaatgcgaa accaccctca gcatcgaaca aaccattatt
    10021 catgcgctgg taacacagct gctggaagag agcaccacgc tcgatcggga agcctttaaa
    10081 aaactgctct ctgagcgcca gagcaaatac tggtgtcaga cacaaccaga gtattacgcc
    10141 atctatgacg cattgcgcca ggctgagcgg ttgctgaacc tgcgcaatcg ccacatcgat
    10201 ggtttccact accaggacag cgccaccttc tggaaagcct actgcgaaga actgttccgc
    10261 ttcgaccagg cttatcgcct gtttaatgaa tatgccttgc tggttcacag caaaggagcg
    10321 atgatcctca agagcctgga tgattatatc gaggcgctct acagcaactg gtatctggca
    10381 gagttaagcc gtaactggaa cgaagtgctg gaagcggaaa atcgtatgca ggcgtggcaa
    10441 atccctggcg tgccgcgtca gcagaacttc ttcaatgagg tggtgaagcc acagttccaa
    10501 aatccgcaaa tcaaacgcgt gttcgtgata atttccgatg ccctgcgtta tgaagtggcg
    10561 gaggagctgg ggaatcaaat caataccgag aaacgcttta ccgcagaact gcgctcgcag
    10621 ctcggcgtgc tccccagcta cacccaactg ggaatggcgg cattgctgcc ccatgaacaa
    10681 ctttgctatc aacccggtaa cggcgacatc gtttatgctg atgggctgtc gacctcgggt
    10741 attcctaacc gcgataccat tctgaagaac tataagggaa tggcgataaa atcgaaggac
    10801 cttctggagt taaaaaatca ggaagggcga gaccttattc gcgattacga agtggtgtat
    10861 atctggcata acacgattga tgccactggc gacacggcat ccacggaaga taaaaccttc
    10921 gaagcgtgcc gcacggcggt ggctgaactg aaagatttag tcaccaaggt gatcaaccgc
    10981 ctccacggca cacgcatttt tgttacggcg gatcacggtt tcctgttcca gcaacaggcg
    11041 ctttcggttc aggataaaac cactctgcaa attaagccgg aaaacaccat caagaaccac
    11101 aaacgcttta ttatcggcca tcagcttccc gccgatgatt tttgctggaa agggaaagtg
    11161 gcggataccg caggcgtgag cgacaacagc gagttcctga ttccgaaagg gatccagcgc
    11221 ttccatttct ctggcggcgc gcgcttcgtt catggcggca ccatgttgca ggaggtttgc
    11281 gttccggtat tgcagataaa agccctgcaa aaaaccgccg cagaaaaaca gccacagcgc
    11341 cgcccggtgg atattgtcgc ttaccatccg atgattaagc tagtgaacaa tatcgataaa
    11401 gtgagcctgt tgcagacgca tccggtgggc gaactttatg aaccgcgtat cctgaacatt
    11461 tacattgtcg acaacgccaa caatgtggtc tcgggcaaag agcgcatcag ctttgacagt
    11521 gataacaaca ccatggaaaa acgcgtacgc gaagttacgc tgaagctgat tggcgctaac
    11581 ttcaaccgtc gcaatgagta ctggttgata ctggaagacg cacaaacgga aacggggtat
    11641 cagaagtacc cggtcattat cgatctggcg ttccaggatg atttcttcta agtgaggcga
    11701 tatgcaaacc catcatgatt tacctgtttc aggcgtatcc gcaggggaaa ttgcctccga
    11761 gggttacgat ctggacgccc tgctgaacca gcattttgct ggtcgcgtgg tgcgtaaaga
    11821 tctcaccaag caactcaagg aaggggcaaa cgtcccggtg tatgtgcttg agtatctgct
    11881 cggcatgtac tgcgcctctg acgatgacga tgtggtcgag caagggttgc aaaacgttaa
    11941 gcgtattctg gctgataact atgtgcgccc ggatgaagcg gagaaagtga agtcgctgat
    12001 ccgcgagcgt ggttcgtaca aaatcatcga taaagtgtcg gtgaaactga accagaaaaa
    12061 agacgtttac gaagcccagc tttctaacct cggcatcaaa gacgcgctgg tgccctcgca
    12121 gatggttaaa gacaacgaga agctactgac gggcggtatc tggtgcatga ttaccgtcaa
    12181 ctatttcttt gaagaagggc agaagacctc acccttctca ttgatgacgc tcaagcctat
    12241 ccagatgccg aatatggata tggaagaggt gttcgatgcg cgtaaacact ttaaccgtga
    12301 ccagtggatc gatgtgctgc tgcgctcggt gggtatggag cccgccaata ttgagcaacg
    12361 caccaaatgg caccttatca cccgtatgat cccgttcgtg gagaacaact ataacgtttg
    12421 cgagctgggg ccgcgtggca ccggtaaaag ccatgtgtat aaagagtgtt ctcctaactc
    12481 cctgttagtt tccggcgggc aaacgaccgt tgccaacttg ttctacaaca tggccagtcg
    12541 ccagatcggc ctggttggca tgtgggatgt ggtagcgttc gacgaagtcg cggggatcac
    12601 tttcaaagat aaagacggcg tgcaaatcat gaaagattac atggcgtcag gatctttctc
    12661 tcgcggcaga gattcgattg aaggtaaagc gtcgatggtt ttcgtcggca acatcaatca
    12721 aagcgtagag actctcgtta aaaccagcca tttgctggca ccatttccga ctgcgatgat
    12781 tgatacagca tttttcgacc gctttcatgc ctatattccc ggttgggaaa tccccaaaat
    12841 gcgcccggaa ttctttacca accgttacgg gctgattacg gattatctcg ctgaatatat
    12901 gcgcgaaatg cgcaaacgca gtttctctga tgcgattgat aaattcttta agctgggtaa
    12961 caacctcaac cagcgtgacg ttattgccgt tcgacgtacc gtgtcggggt tgttaaaact
    13021 catgcatccc gatggcgcgt acagcaaaga agatgtgcga gtctgcctga cctatgcgat
    13081 ggaagttcgc cgccgcgtga aagagcaact taaaaaactg ggcggtctgg agttcttcga
    13141 tgtgaacttt agctacatcg acaacgaaac gctggaagag ttttttgtga gcgtaccgga
    13201 acagggcggc agcgaactta ttcctgccgg aatgccaaag ccgggtgttg tgcatctggt
    13261 cactcaggca gaaagcggca tgaccgggct gtatcgtttt gaaacacaga tgactgccgg
    13321 taatggtaag catagtgtat cgggtctggg ttcaaatacc tccgcgaaag aagctatccg
    13381 cgtcggtttc gattacttca aaggcaattt gaatcgggta agcgcggccg cgaaattctc
    13441 cgatcatgaa tatcaccttc atgtcgttga actgcataat actggcccaa gcaccgcaac
    13501 cagtcttgct gcgcttatcg ctttatgttc gatattgctg gcaaaaccgg tgcaggaaca
    13561 gatggtggtg ttgggcagta tgacgcttgg tggggtaatt aacccggtgc aggatcttgc
    13621 cgccagtttg cagctcgcct tcgacagcgg tgcaaaacgg gttctgttgc cgatgtcctc
    13681 ggctatggat attccaacgg ttccggcaga gttatttacc aagtttcagg tgagttttta
    13741 ctcagacccg gttgatgctg tttataaggc gctgggtgtg aattaacgta gtaactattt
    13801 taatgaaccg caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
    13861 tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
    13921 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac
    13981 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg
    14041 tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
    14101 actcatcgca atactgttgt aattcattta acattctgcc gacatggaag ccatcacaga
    14161 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
    14221 tgcccatagt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
    14281 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
    14341 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
    14401 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
    14461 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcatcgcca
    14521 tacggaactc tggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
    14581 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
    14641 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
    14701 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
    14761 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
    14821 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct
    14881 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag
    14941 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga
    15001 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
    15061 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta
    15121 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag
    15181 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc
    15241 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
    15301 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
    15361 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
    15421 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
    15481 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
    15541 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
    15601 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
    15661 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
    15721 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
    15781 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
    15841 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
    15901 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag
    15961 tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgtaat
    16021 tctcatgtta gtcatgc
//
