LOCUS       Exported               14157 bp ds-DNA     circular SYN 02-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Linyi Gao
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 2, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          106..11928
                     /organism="Escherichia coli"
                     /strain="NCTC9078"
                     /mol_type="genomic DNA"
                     /serovar="not available: to be reported later"
                     /label=contig: ERS523585SCcontig000005
                     /note="contig: ERS523585SCcontig000005"
                     /db_xref="taxon:562"
     CDS             432..1646
                     /codon_start=1
                     /locus_tag="DX464_RS06235"
                     /old_locus_tag="NCTC9078_01271"
                     /product="DUF4338 domain-containing protein"
                     /label=druA
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_000549798.1"
                     /translation="MHKYPSIIVNINLREAKLKKKVREHLQSLGFTRSDSGALQAPGNT
                     KDVIRALHSSQRAERIFANQKFITLRAAKLIKFFASGNEVIPDKISPVLERVKSGTWQG
                     DLFRLAALTWSVPVSSGFGRRLRYLVWDESNGKLIGLIAIGDPVFNLAVRDNLIGWDTH
                     ARSSRLVNLMDAYVLGALPPYNALLGGKLIACLLRSRDLYDDFAKVYGDTVGVISQKKK
                     QARLLAITTTSSMGRSSVYNRLKLDGIQYLKSIGYTGGWGHFHIPDSLFIELRDYLRDM
                     DHAYADHYMFGNGPNWRLRTTKAALNALGFRDNLMKHGIQREVFISQLAENATSILQTG
                     KGEPDLTSLLSAKEIAECAMARWMVPRSIRNPEYRLWKARDLFDFISNDSLNFPPFDEI
                     AKTVV"
     CDS             1668..3314
                     /codon_start=1
                     /locus_tag="DX464_RS06230"
                     /old_locus_tag="NCTC9078_01270"
                     /product="CoiA-like nuclease"
                     /label=druB
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_001315973.1"
                     /translation="MNYAIDKFTGTLILAARATKYAQYVCPVCKKGVNLRKGKVIPPYF
                     AHLPGHGTSDCENFVPGNSIIVETIKTISKRYMDLRLLIPVGSNSREWSLELVLPTCNL
                     CRAKITLDVGGRSQTLDMRSMVKSRQIGAELSVKSYRIVSYSGEPDPKFVTEVERECPG
                     LPSEGAAVFTALGRGASKGFPRAQELRCTETFAFLWRHPVAPDFPDELEIKSLASKQGW
                     NLALVTIPEVPSVESISWLKSFTYLPVVPARTSITAIWPFLNQKTSINHVECVYSDTIL
                     LSTNMAPTSSENVGPTMYAQGSSLLLSAVGVETSPAFFILNPGENDFVGVSGSIEQDVN
                     LFFSFYKKNVSVPRKYPSIDLVFTKRNKEKTIVSLHQRRCIEVMMEARMFGHKLEYMSM
                     PSGVEGVARIQRQTESNVIKLVSNDDIAAHDKSMRLLSPVALSQLSDCLANLTCHVEID
                     FLGLGKIFLPGSSMLSLDDGKFIELSPNLRSRILSFILQMGHTLHGFSLNNDFLLVEKL
                     VDLQPEPHLLPHYRALVKEVKTNGFECNRFR"
     CDS             3326..5209
                     /codon_start=1
                     /locus_tag="DX464_RS06225"
                     /old_locus_tag="NCTC9078_01269"
                     /product="hypothetical protein"
                     /label=druC
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_021520530.1"
                     /translation="MSYQYSQEAKERISKLGQSEIVNFINEISPTLRRKAFGCLPKVPG
                     FRAGHPTEIKEKQKRLIGYMFQSHPSSEERKAWKSFSLFWQFWAEEKIDKSFSMIDNLG
                     LKENSGSIFIRELAKNFPKVARENIERLFIFSGFADDPDVINAFNLFPPAVVLARDIVI
                     DTLPIRLDELEARISLIADNVEKKNNHIKELELKIDAFSEQFDNYFNNEKSSLKIINEL
                     QSLINSETKQSDIANKAIDELYHFNEKNKQLILSLQEKLDFNALAMNDISEHEKLIKSM
                     ANDISEFKNALTILCDNKIKNNELDYVNELKKLTERIDTLEINTSQASEVSVTNRFTKF
                     HEIAHYENYEYLSSSEDISNRISLNLQAVGLTKNSAEKLARLTLATFVSGQIIQFSGSL
                     ADIIADAIAIAIGAPRYHIWRVPVGIISDMDAFDFIETIAESSRCLLLKGANLSAFEIY
                     GAAIRDIVVQRQIHPTNYDHLALIATWKQGPATFPDGGMLAELGPVIDTDTLKMRGLSA
                     TLPQLKPGCLAKDKWTNIDGLHLDSVDDYVDELRALLDEAGFDGGTLWKRMIHIFYTSL
                     IRIPNGNYIYDLYSVLSFYTLTWAKIKGGPVQKIEDIANRELKNYSAKISS"
     gene            3326..5209
                     /locus_tag="DX464_RS06225"
                     /old_locus_tag="NCTC9078_01269"
                     /label=DX464_RS06225
     CDS             5221..6264
                     /codon_start=1
                     /locus_tag="DX464_RS06220"
                     /old_locus_tag="NCTC9078_01268"
                     /product="hypothetical protein"
                     /label=druD
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_000455180.1"
                     /translation="MEWRAVSRDKALDMLSTALNCRFDDEGLRISAVSECLRSVLYQYS
                     ISETEEARQTVTSLRLTSAVRRKLVPLWPDIADIDNAIHPGIMSILNSLAELGDMIKLE
                     GGNWLTAPPHAVRIDNKMAVFFGGEPSCTFSTGVVAKSAGRVRLVEEKVCTGSVEIWDA
                     NEWIGAPAEGNEEWSSRLLSGTISGFIDAPGNMSETTAYVRGKWLHLSELSFNKKQIYL
                     CRMSVDNHFSYYLGEIEAGRLCRMNSLESSDDVRRLRFFLDTKDNCPLKVRIKISNGLA
                     RLRLTRRLPRRETKVLLLGWRESGFENEHSGITHHVFPEEILPIVRSAFEGLGIIWINE
                     FTRRNEI"
     CDS             6261..11771
                     /codon_start=1
                     /locus_tag="DX464_RS06215"
                     /old_locus_tag="NCTC9078_01267"
                     /product="DEAD/DEAH box helicase"
                     /label=druE
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_000608843.1"
                     /translation="MINKNKVTERSGIHDTVKSLSENLRKYIEAQYHIRDEGLIAERRA
                     LLQQNETIAQAPYIEATPIYEPGAPYSELPIPEAASNVLTQLSELGIGLYQRPYKHQSQ
                     ALESFLGENASDLVIATGTGSGKTESFLMPIIGKLAIESSERPKSASLPGCRAILLYPM
                     NALVNDQLARIRRLFGDSEASKILRSGRCAPVRFGAYTGRTPYPGRRSSRRDELFIKPL
                     FDEFYNKLANNAPVRAELNRIGRWPSKDLDAFYGQSASQAKTYVSGKKTGKQFVLNNWG
                     ERLITQPEDRELMTRHEIQNRCPELLITNYSMLEYMLMRPIERNIFEQTKEWLKADEMN
                     ELILVLDEAHMYRGAGGAEVALLIRRLCARLDIPRERMRCILTSASLGSIEDGERFAQD
                     LTGLSPTSSRKFRIIEGTRESRPESQIVTSKEANALAEFDLNSFQCVAEDLESAYAAIE
                     SLAERMGWQKPMIKDHSTLRNWLFDNLTGFGPIETLIEIVSGKAVKLNILSENLFPDSP
                     QQIAERATDALLALGCYAQRASDGRVLIPTRMHLFYRGLPGLYACIDPDCNQRLGNHSG
                     PTILGRLYTKPLDQCKCASKGRVYELFTHRDCGAAFIRGYVSSEMDFVWHQPNGPLSED
                     EDIDLVPIDILVEETPHVHSDYQDRWLHIATGRLSKQCQDEDSGYRKVFIPDRVKSGSE
                     ITFDECPVCMRKTRSAQNEPSKIMDHVTKGEAPFTTLVRTQISHQPASRPIDGKHPNGG
                     KKVLIFSDGRQKAARLARDIPRDIELDLFRQSIALACSKLKDINREPKPTSVLYLAFLS
                     VLSEHDLLIFDGEDSRKVVMARDEFYRDYNSDLAQAFDDSFSPQESPSRYKIALLKLLC
                     SNYYSLSGTTVGFVEPSQLKSKKMWEDVQSKKLNIESKDVHALAVAWIDTLLTEFAFDE
                     SIDSTLRIKAAGFYKPTWGSQGRFGKALRKTLIQYPAMGELYVEVLEEIFRTHLTLGKD
                     GVYFLAPNALRLKIDLLHVWKQCNDCTALMPFALEHSTCLACGSNSVKTVEPSESSYIN
                     ARKGFWRSPVEEVLVSNSRLLNLSVEEHTAQLSHRDRASVHATTELYELRFQDVLINDN
                     DKPIDVLSCTTTMEVGVDIGSLVAVALRNVPPQRENYQQRAGRAGRRGASVSTVVTYSQ
                     NGPHDSYYFLNPERIVAGSPRTPEVKVNNPKIARRHVHSFLVQTFFHELMEQGIYNPAE
                     KTAILEKALGTTRDFFHGAKDTGLNLDSFNNWVKNRILSTNGDLRTSVAAWLPPVLETG
                     GLSASDWFAKVAEEFLNTLHGLAEIVPQTAVLVDEENEDDEQTSGGMKFAQEELLEFLF
                     YHGLLPSYAFPTSLCSFLVEKIVKNIRGSFEVRTVQQPQQSISQALSEYAPGRLIVIDR
                     KTYRSGGVFSNALKGELNRARKLFNNPKKFIHCDKCSFVRDPHNNQNSENTCPICGGIL
                     KVEIMIQPEVFGPENAKELNEDDREQEITYVTAAQYPQPVDPEDFKFNNGGAHIVFTHA
                     IDQKLVTVNRGKNEGESSGFSVCCECGAASVYDSYSPAKGAHERPYKYIATKETPRLCS
                     GEYKRVFLGHDFRTDLLLLRITVGSPLVTDTSNAIVLRMYEDALYTIAEALRLAASRHK
                     QLDLDPAEFGSGFRILPTIEEDTQALDLFLYDTLSGGAGYAEVAAANLDDILTATLALL
                     ESCECDTSCTDCLNHFHNQHIQSRLDRKLGASLLRYALYGMVPRCASPDIQVEKLSQLR
                     ASLELDGFQCIIKGTQEAPMIVSLNDRSIAVGSYPGLIDRPDFQHDVYKSKHTNAHIAF
                     NEYLLRSNLPQSHQNIRKMLR"
     terminator      11937..11972
                     /label=T7 Terminator
     CDS             complement(12204..12863)
                     /codon_start=1
                     /label=CAT/CamR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(12864..12966)
                     /label=cat Promoter
     misc_feature    13225..14137
                     /label=p15A_origin
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaggtga acgtttggtt
      121 gatagggtag taaaactagt aatcatccta taattagcta tattcgtggt tattagattg
      181 aaaacagata acattaacaa aatctataaa tcgatttgaa tgattttttt catcaatact
      241 gttgtaagct cctgctatca aaagttttgc acacaatcta taagctccca gaattgcttg
      301 tataaatgct atcattggcg ctgtcccgat cgagggagca aggaggggac tctcttgtgc
      361 catgcgatta atcactgggg ctctaagtga aatttagtgg gactaaatac taattggaac
      421 gtgagataaa aatgcacaaa tatccctcta taatagttaa tatcaacctt cgagaagcca
      481 aactgaaaaa gaaggtacgt gagcatttac aatccttggg ttttacaaga tctgattctg
      541 gagcgctcca ggccccggga aataccaaag atgtaatacg ggctcttcat agttctcaac
      601 gagctgagcg gatatttgca aaccaaaagt tcataacgct aagagcggca aagcttatta
      661 aatttttcgc atccggcaat gaggtcattc cggataagat ttcaccggta cttgaacgtg
      721 taaagtcagg aacctggcaa ggagatctct ttaggttagc agcattaact tggtccgtac
      781 ctgtttcaag cggatttgga aggcgtctcc ggtatcttgt atgggatgaa agcaacggaa
      841 aattgatagg gctgatcgca attggtgacc ctgtgttcaa ccttgcagtc cgagataatt
      901 tgattgggtg ggatactcat gccagaagtt cccggcttgt taatttgatg gatgcatacg
      961 tcctcggtgc tcttccccct tataatgccc tgctgggagg aaaattaatt gcatgtctgc
     1021 ttcgtagccg cgatctttat gatgactttg caaaggtcta tggtgatacc gttggagtaa
     1081 tatctcaaaa aaagaaacaa gcacgtcttt tggctattac aacaacatcg tctatggggc
     1141 gctcatcggt atataaccgt ttaaagctgg atggaattca atatttaaaa tcgattggat
     1201 atacaggcgg ttgggggcat tttcatatac ctgatagctt gttcattgaa ttacgtgatt
     1261 acttacgtga tatggatcac gcttatgcag atcattatat gtttggtaat gggcctaact
     1321 ggcgtttacg tacaactaag gcagctttaa atgcactagg atttagagat aatttgatga
     1381 agcatggaat tcaacgtgaa gtgtttatca gtcagctagc agaaaatgca actagtattc
     1441 tgcaaacagg caaaggtgaa ccagatctaa cctctttgct ttctgctaaa gagatagctg
     1501 agtgtgcgat ggcacgatgg atggttccac gatcaattcg caatccagaa tatcggcttt
     1561 ggaaagcaag agatctattt gattttatta gtaatgactc gctaaacttt cccccgtttg
     1621 acgagatagc gaaaacagtt gtctaatctt aactgaaggg ggagtaagtg aattacgcta
     1681 ttgataagtt caccgggaca ctgatattag cagctcgagc aacgaaatat gctcaatatg
     1741 tttgcccagt ttgtaaaaaa ggtgttaacc tccgtaaagg gaaggttata cccccatatt
     1801 ttgctcattt gcccggacat ggtacgtcag actgtgaaaa ttttgttccc ggaaattcta
     1861 tcattgtcga aactattaaa actatttcaa agcgatatat ggatttgcgc ttattgattc
     1921 ctgtcggaag taatagtcga gagtggtcat tagaattagt gttgccaacc tgtaatttat
     1981 gtagagcaaa gataacgtta gatgtaggag gcagaagcca aacgcttgat atgaggagta
     2041 tggtaaagag tcgccagatt ggtgctgaat tatcagtaaa atcttaccgt attgtttcat
     2101 atagtggtga accagatcca aaatttgtaa cagaagttga aagagaatgc ccaggtttac
     2161 cttctgaggg agcagcagtt ttcactgctt tagggcgtgg ggcatcgaag ggatttccac
     2221 gagcacaaga gttaagatgt actgaaacat ttgcctttct ttggcgacac cctgttgctc
     2281 cagattttcc tgatgaatta gaaataaaaa gtttagctag taaacaggga tggaatttag
     2341 ctcttgttac aattcctgaa gtcccttctg tggagagtat ttcatggcta aaatctttta
     2401 cataccttcc tgttgttcct gccagaacat ctattacagc aatttggccg ttcctaaatc
     2461 aaaaaacaag tattaatcat gtcgaatgtg tttattctga cacaatattg ttgtcaacaa
     2521 atatggcacc aacatcatca gaaaatgttg gaccaactat gtacgcacaa ggttcctctt
     2581 tattactttc agcggttggt gttgaaacat cacctgcttt cttcattcta aatcctggag
     2641 aaaatgactt tgtgggcgtt tctggctcaa ttgagcagga cgtaaactta tttttttctt
     2701 tctataaaaa aaacgtttct gtacccagaa aatatccctc aatagatttg gtttttacta
     2761 agaggaataa agaaaagacc atcgtttcct tacatcaaag aagatgcatt gaagttatga
     2821 tggaagcacg aatgtttggc cataaattag aatacatgtc tatgccttct ggtgttgaag
     2881 gagtggcaag aattcaaaga caaactgaaa gtaatgttat taagttagtt tctaatgatg
     2941 acattgcagc tcatgataag agcatgcggt tactatctcc tgttgcgtta tctcaattat
     3001 ctgattgctt agcaaactta acatgtcatg tagaaataga ttttttaggt cttggtaaaa
     3061 tatttttacc tggttcttct atgctatcat tagatgacgg gaaatttatt gaattatctc
     3121 ctaatcttcg ctcacggata ttaagtttta tacttcaaat ggggcacacc ctccatggtt
     3181 ttagtttaaa taatgatttt ttattagttg agaaattagt ggatttgcag ccggaaccac
     3241 acttattacc gcattataga gcattggtaa aagaagttaa gaccaatgga tttgaatgta
     3301 accgctttag ataaggtgcc ttcgaatgag ttaccaatat agccaagagg caaaggaacg
     3361 gatctctaag ttgggacaat ccgaaattgt taactttatc aatgagattt ctccaacttt
     3421 acgacgtaaa gcttttggtt gtttaccaaa agtaccggga ttcagggcag gacatcccac
     3481 tgaaattaaa gaaaaacaga aaagattgat tgggtatatg ttccagtcac atccttcctc
     3541 tgaggagaga aaagcatgga aaagtttttc tcttttttgg cagttttggg ctgaagagaa
     3601 aattgacaaa tcatttagta tgattgataa tttaggatta aaagaaaact ctggctctat
     3661 ttttattaga gagcttgcta aaaactttcc taaagttgct agagagaata tcgagcgcct
     3721 gtttatcttt agtgggtttg ctgatgatcc agacgttata aatgcattta acctttttcc
     3781 tcctgcagtt gttcttgccc gcgatatcgt gattgatact cttccaattc gtttagatga
     3841 gcttgaagca cgtattagtt taattgccga taatgttgag aaaaaaaata atcatattaa
     3901 agaacttgag ttaaaaatag atgctttttc cgaacagttt gataattact ttaataatga
     3961 aaagagcagt ttaaaaataa ttaatgaact acaatctttg ataaactcag agactaaaca
     4021 atctgatatt gctaataaag ctattgacga gctttatcat tttaatgaaa aaaacaaaca
     4081 gctaatatta tctcttcaag aaaaattaga ttttaatgct ctggctatga atgatatttc
     4141 tgagcatgaa aaattgataa aaagtatggc taatgacatt tcagaattta aaaatgcatt
     4201 aacgatcttg tgtgataata aaataaagaa taacgagtta gattatgtca atgaattaaa
     4261 aaaactcact gaacgaatag atacacttga aataaacaca tctcaagcta gcgaagtgag
     4321 tgtcaccaat agatttacaa aattccatga aatagcgcac tatgaaaatt atgagtatct
     4381 ttcatcctcc gaagacatat ctaatagaat ttctttaaat ttacaggctg ttggattgac
     4441 aaaaaattca gcagaaaaat tggctagatt gacattagct accttcgttt ctggacaaat
     4501 cattcaattc agtggctctt tggcagatat tatcgcggat gcaattgcca ttgctattgg
     4561 tgcaccacgt tatcacatat ggagagttcc agttggtatt atttctgaca tggatgcttt
     4621 tgattttata gagactatag ctgaatcatc tcgctgtctc cttttgaaag gggccaatct
     4681 ttcagcattt gagatttatg gagcggcaat tagagatata gttgttcaac ggcaaataca
     4741 tccaacaaat tatgaccatc tggcattgat agctacctgg aaacaaggcc cagctacatt
     4801 ccctgatgga ggaatgttgg ccgagttggg acctgttatt gatactgata cattaaaaat
     4861 gcgtggttta tcagctactt taccccaatt gaaaccaggt tgtcttgcca aggataaatg
     4921 gacaaatatt gatggactac atcttgatag tgttgatgat tatgtagatg aattaagagc
     4981 attactggac gaagctggat ttgatggggg aactttgtgg aagagaatga ttcatatttt
     5041 ctatacttca ctcataagga tccctaatgg aaattatatt tatgatcttt attctgtctt
     5101 gtctttttat actcttacat gggcaaaaat taaaggtggc cccgtccaaa agatagaaga
     5161 tattgccaat cgtgaattaa aaaattatag tgcaaaaata tcttcttgag gaggtggtta
     5221 atggagtgga gagcagtatc acgagacaaa gcactggata tgttatcaac tgcattaaat
     5281 tgtcgatttg atgatgaagg gttgagaatt tcagcagttt cagaatgctt aaggagcgta
     5341 ttatatcaat attctatatc tgaaacagaa gaagctaggc aaactgtaac ctcgcttcga
     5401 ctcactagtg cagtaaggcg aaaattggta cctttatggc cagacattgc tgatattgat
     5461 aatgctatac atccgggcat tatgtctata ttgaacagct tggctgaatt gggtgacatg
     5521 attaagttag aaggtggtaa ttggctaaca gctcccccac atgcagtacg aattgacaat
     5581 aagatggctg ttttttttgg tggagagcct tcctgtacat tttcaacggg cgtggtagct
     5641 aaatctgctg gaagagttcg cttggttgaa gaaaaagtgt gtactggaag tgttgaaatc
     5701 tgggatgcaa atgagtggat tggtgcccca gcagaaggca atgaagaatg gtcatccaga
     5761 ctactatctg gaactatttc cggctttatc gatgcacctg gcaatatgag tgaaacgact
     5821 gcatatgtgc ggggaaaatg gctccatttg tcagaacttt cttttaataa aaagcaaatc
     5881 tacttatgca gaatgtccgt tgataatcac ttttcctatt atttaggaga aattgaagct
     5941 ggacgcttat gtagaatgaa ttcgttagaa tcgtctgatg atgtcagaag attacgtttt
     6001 tttctcgata caaaagataa ttgtccgcta aaggtccgta tcaaaatatc taatgggcta
     6061 gcaagattaa gattaaccag aagattacca agacgagaaa cgaaggtact cctgctaggc
     6121 tggagagaat caggttttga aaatgaacat tcaggaataa cacaccatgt attccccgag
     6181 gaaatattac ccatagtgcg tagcgctttt gaagggcttg gtattatttg gattaacgaa
     6241 ttcacgcgac ggaatgaaat atgattaata aaaataaagt aactgaacgt tcaggtatac
     6301 atgataccgt gaaaagcctt agtgaaaatc tgagaaaata cattgaggca caatatcata
     6361 tccgggatga agggttaatt gctgagcgac gagcgctttt acagcaaaat gaaactattg
     6421 ctcaagctcc ttatatagaa gcaaccccaa tttatgaacc tggtgcgcca tacagtgaat
     6481 tgcctattcc cgaagcagca agtaatgtgc taactcaact atcagaactt ggaattggcc
     6541 tctatcaacg cccctataaa caccaatcac aggcacttga gtcatttctt ggcgaaaacg
     6601 cttctgatct ggtcattgca acaggtacag gctccggtaa gactgaaagc tttctaatgc
     6661 caattattgg aaaattggcg attgaatctt ccgagagacc taaatctgca tcccttccag
     6721 gttgtagagc aattttatta tatccaatga atgcattagt taacgatcaa cttgctcgta
     6781 tcagacgtct ttttggtgat tctgaagcct ctaaaatact gagatctgga agatgtgccc
     6841 ctgtacgctt tggcgcttat acgggaagaa cgccttaccc tggtcgtcgt agctctagac
     6901 gagacgagct ttttatcaaa ccccttttcg atgagtttta caataaactc gcaaataacg
     6961 cccccgtacg tgcggaactg aaccgcattg gtcgctggcc aagtaaagat cttgatgctt
     7021 tttatgggca aagcgcatct caggctaaaa cctacgtctc aggcaaaaaa acgggtaagc
     7081 aatttgtttt gaacaattgg ggggagaggc taattaccca gcctgaggat cgtgagctaa
     7141 tgacccggca tgaaatacag aatcgctgtc cagaattact gataacgaac tactccatgc
     7201 ttgagtatat gctgatgcga cctatcgagc gtaatatttt tgagcagact aaggaatggc
     7261 tcaaagctga tgagatgaat gagcttatct tagtgcttga tgaagcgcat atgtatagag
     7321 gagcaggggg agcagaggta gcccttttaa tacgtcgcct ctgtgctcgg ttggatattc
     7381 cccgggaacg tatgcgctgc atccttacca gtgctagtct agggtccatt gaggatggag
     7441 aacgttttgc ccaagactta actggcttat caccaacctc ttcgaggaaa tttcgaatta
     7501 ttgagggtac aagggaatcg cgtcctgagt cacaaattgt taccagtaaa gaagctaatg
     7561 cactggctga attcgaccta aattcatttc agtgcgtagc tgaggatctt gaatctgcat
     7621 atgcagcaat agagtctctt gccgaacgaa tgggctggca aaagccgatg ataaaagatc
     7681 atagtacact acgtaattgg ttatttgata atttgactgg ttttggtcct attgaaacgc
     7741 ttattgaaat agtttcaggt aaagcggtta agctaaatat cttgagtgaa aacctttttc
     7801 cagactctcc acagcaaatc gcagagcgag caacagatgc attactcgca ttgggttgct
     7861 atgctcagag ggcatccgat ggcagagtgc ttattccaac tcgcatgcat cttttttatc
     7921 ggggattacc aggtctttat gcctgtatag atcccgattg taatcaacgt ttgggtaacc
     7981 atagcgggcc aactatactt ggccgccttt atacgaaacc actggatcaa tgtaaatgcg
     8041 cttcaaaagg gcgagtctac gaattattta cccaccgtga ctgcggtgcg gcttttattc
     8101 gtggatacgt tagttccgaa atggactttg tatggcacca gccgaacgga ccattatcag
     8161 aagatgagga tatcgatctt gttcccatag atatattggt cgaggaaaca cctcatgtac
     8221 atagtgatta ccaggacaga tggctacata tagcaacagg acgcctttct aaacagtgtc
     8281 aagatgagga ttctggttat cgtaaagtct ttatacctga ccgagttaag tctggatcag
     8341 aaattacatt tgatgaatgc cctgtttgta tgcgtaagac aagaagtgct cagaatgaac
     8401 cgtctaaaat tatggatcat gttacaaaag gggaagcacc ttttacaacg ttagtacgta
     8461 cacagatatc tcaccagcca gcgagtcgtc ctattgatgg taaacatccc aatgggggaa
     8521 aaaaagtact tattttttct gatggccgac aaaaagcagc tcggcttgca cgtgatattc
     8581 ctagagatat tgagcttgat ttgtttcggc aatccattgc tctcgcctgt tctaaactga
     8641 aagatatcaa tcgggaaccc aaaccaacat cagtacttta ccttgctttc ctatcagtcc
     8701 tttctgaaca tgacttgctt atttttgatg gggaagattc acgaaaagtt gtaatggccc
     8761 gtgatgaatt ttatcgtgat tataatagcg atctggctca agcttttgat gatagcttca
     8821 gcccccaaga gtcaccgtca cgatataaaa tagcgttgct taaactttta tgtagcaatt
     8881 actattctct ttccggaaca acagttggtt ttgttgaacc atcgcagctt aaatcaaaaa
     8941 aaatgtggga agatgtgcag tccaagaagc tcaatattga gagcaaggat gttcatgctt
     9001 tagctgttgc ttggattgat accttactca ctgaatttgc ttttgatgaa tctattgatt
     9061 cgacactacg aatcaaagca gctggattct acaaacccac ttggggtagt caaggacggt
     9121 ttggaaaagc tcttaggaaa accctgatac agtatcctgc tatgggggag ctttatgtgg
     9181 aagttttgga ggagattttt cgtactcatc tgacattagg aaaagatggt gtctactttc
     9241 ttgctccaaa tgcactacgt ctgaaaatag atctcttgca tgtctggaaa caatgtaatg
     9301 actgcacggc actaatgcca tttgctttag aacattctac ttgccttgct tgtggtagta
     9361 acagtgtcaa aacagtcgag ccgtcggaaa gcagctatat taatgcacga aaaggattct
     9421 ggcgttcgcc ggtagaagaa gttttggttt caaattcgcg gcttctaaac cttagcgttg
     9481 aagagcatac tgctcaactc tcacatagag atagggccag cgttcatgcc actacagaac
     9541 tctacgaact gagattccaa gatgttctta ttaatgataa cgacaagccc attgatgtac
     9601 ttagttgtac gacgacgatg gaagtggggg ttgatattgg atctctggtt gctgttgctt
     9661 taagaaacgt ccctccgcaa cgagaaaatt atcagcaacg tgctgggcga gcaggccgcc
     9721 gtggcgcatc tgtttcaacg gtggttacat attctcaaaa tggccctcat gatagttatt
     9781 atttccttaa tcctgaacgc attgttgcag gttctcctcg tacacctgaa gtgaaagtaa
     9841 ataatcccaa aatagccaga agacacgttc attctttttt agttcagacc ttttttcacg
     9901 agttaatgga acaaggaatt tataatcccg cagagaaaac tgccatactt gagaaagcac
     9961 ttggtactac acgagatttt tttcatggag caaaagatac tggcctaaat ctcgatagct
    10021 ttaataattg ggttaaaaac cgtattctat ctactaatgg tgatttgaga acaagtgttg
    10081 cagcatggct tcctcctgtt cttgaaactg gagggctttc tgccagtgac tggtttgcta
    10141 aggtagcaga ggaattttta aatacactcc atgggctggc tgaaattgtt ccacaaactg
    10201 ccgttcttgt tgatgaggaa aatgaagatg atgagcagac ttctggtgga atgaaatttg
    10261 cacaagaaga attacttgag ttcctgtttt accatggttt attaccaagt tatgcatttc
    10321 ctacaagcct ctgtagtttc ttggtagaaa aaattgtaaa gaatattaga ggttcttttg
    10381 aggtgcgaac agtacaacag cctcagcaat caatttctca ggctctgagt gaatatgccc
    10441 cgggacgttt gattgttatt gataggaaaa cctatcgctc tggtggtgtt ttttctaatg
    10501 cattgaaagg cgaactaaac cgggcaagaa agcttttcaa taatcccaaa aagtttattc
    10561 attgcgataa gtgctctttt gtccgcgatc ctcataataa tcagaatagc gaaaatactt
    10621 gtccgatctg tggtggcatt ctaaaagtag aaataatgat tcagcccgaa gtctttggac
    10681 ctgaaaatgc caaggaactt aatgaggacg acagagagca agaaatcacc tatgtaacag
    10741 cggcacaata tccacaacct gttgatcctg aagattttaa gttcaataat ggaggtgctc
    10801 atattgtttt tactcacgca atagatcaga aactggtgac ggtgaaccga gggaaaaatg
    10861 agggggagtc cagtggtttt tcagtatgtt gcgaatgtgg tgcggcctcc gtttatgatt
    10921 cctactcacc ggcaaagggg gcacatgaaa gaccgtataa atatatagca actaaggaaa
    10981 cgcctcgctt atgctctggc gagtataaac gcgtttttct cggacatgat ttccgtactg
    11041 atttgctttt attacgaata accgttgggt ctccgcttgt aactgatact tcaaatgcta
    11101 tcgttttacg gatgtatgaa gatgcattat atacaatagc ggaagcacta aggcttgcag
    11161 ctagtcgcca taaacaactg gatcttgatc ctgctgagtt tggctctggt ttcagaattt
    11221 tacccactat agaggaagat actcaggcat tggatctctt cctttatgat actttatccg
    11281 gcggtgcggg ttatgcggaa gtagcagcag cgaatctaga tgacattctt actgcaacac
    11341 tcgcattgtt agaaagctgt gagtgcgata cctcctgtac agattgtctc aatcatttcc
    11401 acaaccagca tatacaaagc cgtctcgata ggaaactagg tgcatcttta cttcgttatg
    11461 cactatacgg aatggttcct cgttgtgctt cacctgatat tcaggtagaa aaattgtctc
    11521 aattgagggc aagtctggaa ttggatggtt ttcaatgcat aattaaggga actcaggagg
    11581 cacctatgat tgtgagtttg aatgaccgtt ctattgcagt gggaagttat cctggtctta
    11641 ttgatcgacc cgactttcaa cacgacgtat ataagtcaaa gcatactaat gctcatatag
    11701 cctttaatga atatcttctt cgttcaaatc tgccacaatc gcatcaaaat attagaaaaa
    11761 tgttgcgctg atagcagcag tattgagtgc cctaaagccc tgtagggcac tcaaggtttt
    11821 cagtgcgtga gcgggcttta actgaagcca taaatgtacg tatgggagaa aatgtgacca
    11881 tttaactcgc cagcaactat tgcacaatgt aaaattatgc ccattgagcg caataactag
    11941 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa cctcaggcat
    12001 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga
    12061 cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga
    12121 atttctgcca ttcatccgct tattatcact tattcaggcg tagcaccagg cgtttaaggg
    12181 caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca atactgttgt
    12241 aattcattta acattctgcc gacatggaag ccatcacaga cggcatgatg aacctgaatc
    12301 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatagt gaaaacgggg
    12361 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
    12421 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
    12481 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
    12541 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
    12601 acactatccc atatcaccag ctcaccgtct ttcatcgcca tacggaactc tggatgagca
    12661 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
    12721 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
    12781 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
    12841 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
    12901 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
    12961 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca
    13021 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt
    13081 gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc
    13141 agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa
    13201 cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc
    13261 actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc
    13321 gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct
    13381 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
    13441 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
    13501 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
    13561 gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt
    13621 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
    13681 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
    13741 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa
    13801 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg
    13861 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca
    13921 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc
    13981 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa
    14041 gatcatctta ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat
    14101 gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgc
//
