LOCUS       Exported                4398 bp ds-DNA     circular SYN 02-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 2, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          106..2169
                     /organism="Shigella dysenteriae"
                     /strain="NCTC2966"
                     /mol_type="genomic DNA"
                     /isolation_source="NA"
                     /serovar="NA"
                     /label=contig: ERS1932669SCcontig000011
                     /note="contig: ERS1932669SCcontig000011"
                     /db_xref="taxon:622"
     CDS             441..2069
                     /codon_start=1
                     /locus_tag="EJK89_RS05470"
                     /old_locus_tag="NCTC2966_01146"
                     /product="TIR domain-containing protein"
                     /label=RT
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_005025120.1"
                     /translation="MSLHDKLLMHNFALANKKSPDFISELPQIEPKPYSNGHKIKWINH
                     TLTSTEVTPPDNLIKICILIESGEIAITSVSDIANLLGVPAGQLLYILYRKKDNYRTFE
                     IEKKNGKKRVINAPCGGLSILQTRLKPVLEYFYRPKKSAHGFIKGKSIITNAGMHIKKN
                     FVVNIDLENYFESISFARVYGIFKSKPFNFAHPAATVLAQLCTHNGKLPQGACTSPILA
                     NIASASLDKQLTQFAGRKKISYSRYADDITFSFNQRNIDIIKKNDDGSYSLSETIDNII
                     SKNGFKINYDKFRVQTRNTRQSVTGLVVNDKVNINRRYIRITRSMIHRWTDDKLKYALL
                     FATEKGYQAKDNNHAIQIFRNHIYGRLSFIKMVRGKDYPGYLKLMSYMSHNDPLKTQEG
                     LRAMKETENFDVFICHASEDKKDIAIPIYDELTKLKISAFIDHVEIKWGDSLIDKINAA
                     LVKSKYVIAILSANSVNKEWPQKELRAVLASEISSGDVKLLTLLKKEDEEVVNLSLPLL
                     SDKFYMVYDNNPEVVANNIKSLLQR"
     misc_feature    join(1179..1181,1185..1190)
                     /label=(Y/F)xDD
     misc_feature    1884..1886
                     /label=Conserved E (TIR)
     terminator      2178..2213
                     /label=T7 Terminator
     CDS             complement(2445..3104)
                     /codon_start=1
                     /label=CAT/CamR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3105..3207)
                     /label=cat Promoter
     misc_feature    3466..4378
                     /label=p15A_origin
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaagtta atgactattg
      121 tgagcgagaa acgcgctact actatatata gacagacaag atgcacttac tgaataaata
      181 ctcataacgg agaaaccagc tgtatagtga acaatagatt tccagtagca tatttttact
      241 tcacttttag ttattaatat gataatcata aactacggct ctgccttaaa tttgtgaggt
      301 tgtttcgcct cgaaggaact aatgttagga catacgccac cgttcagtcg atggtaacgc
      361 ttcttaacta gtggtccgct aagtgatgcg caaagtgatt gggcagagcc gaaacgttta
      421 caatccgata ggagttggtt ttgtcgctac atgataaatt attaatgcat aacttcgcat
      481 tagccaataa aaaaagccct gacttcatat ctgaacttcc tcaaattgaa cctaaaccat
      541 acagcaatgg acataaaatt aaatggataa accacacact tactagcact gaagttactc
      601 cccctgataa cctgattaaa atatgcatat tgattgagtc aggggaaatt gctataacat
      661 cagtaagtga tattgccaat ttacttggag ttcctgctgg ccaattactt tatatactat
      721 atcgtaaaaa agataattat cgtacttttg aaatagaaaa gaagaatggt aaaaaaagag
      781 tcattaatgc tccttgtggc ggtctatcga tactccaaac gagactaaag cccgttcttg
      841 aatatttcta caggccaaag aaatctgctc atggttttat aaaaggaaag agcatcatta
      901 ctaatgctgg gatgcatatt aaaaaaaatt ttgtcgtaaa cattgatcta gaaaactatt
      961 tcgaatcaat aagttttgct agggtttatg gaatatttaa aagtaaacct tttaattttg
     1021 ctcatcctgc agctactgtt ttagctcagt tatgtactca caatggaaaa ttacctcaag
     1081 gtgcgtgtac atcgccaata ttagcaaata ttgcatcagc ttctctagac aaacagctca
     1141 cccaatttgc aggaagaaaa aaaatatctt attctaggta tgctgacgac ataacttttt
     1201 ctttcaatca gagaaatatt gatataatca aaaaaaacga cgacggaagt tatagtctta
     1261 gtgaaactat agacaatatt atttcaaaaa atggctttaa aataaattat gataaattta
     1321 gagttcaaac cagaaataca agacaaagtg ttactggctt agtggttaat gataaagtta
     1381 acattaacag aagatatata agaattacac gttcaatgat tcatagatgg acagatgata
     1441 agctaaagta tgcacttctc tttgctacag aaaaaggata tcaggcaaag gataataacc
     1501 acgcaattca aattttccga aatcatattt atggaaggct tagctttata aaaatggtta
     1561 gagggaaaga ctatccagga tatttaaaac tgatgtcata catgagtcat aacgatccat
     1621 taaaaaccca agaaggattg cgagcaatga aagaaacaga aaactttgat gtttttatat
     1681 gccatgcaag cgaagacaaa aaagacattg caattccaat atatgacgag ttaactaaac
     1741 ttaaaatttc agccttcata gatcatgttg agataaaatg gggcgactcc ttaattgata
     1801 aaataaatgc agcactagtt aaatcaaaat atgtcatcgc tattttatct gctaattcag
     1861 tcaataagga atggcctcaa aaagaattaa gagcagtttt agccagcgaa atatcgagtg
     1921 gcgacgtaaa acttttgacc ttattaaaaa aagaagacga ggaggtcgta aacctatcat
     1981 tacctttact tagtgataag ttttatatgg tctatgataa taatcctgaa gtagtcgcca
     2041 acaatattaa atcactctta caacgataat tctctcacaa aagaaaatgt gcagattgat
     2101 gcgtattaag tattaatctg cacatacaaa aaaaataata aaataataca tttttcataa
     2161 cttgtaggtc gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     2221 ttttgctgaa acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata
     2281 aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga
     2341 ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc
     2401 gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc
     2461 cactcatcgc aatactgttg taattcattt aacattctgc cgacatggaa gccatcacag
     2521 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     2581 ttgcccatag tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     2641 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     2701 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     2761 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     2821 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcatcgcc
     2881 atacggaact ctggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     2941 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     3001 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     3061 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     3121 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     3181 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
     3241 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
     3301 ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat ttagtgtatg
     3361 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
     3421 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct agcggagtgt
     3481 atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga
     3541 gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct
     3601 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     3661 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
     3721 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     3781 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
     3841 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     3901 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     3961 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
     4021 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
     4081 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
     4141 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
     4201 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
     4261 agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca
     4321 gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa
     4381 ttctcatgtt agtcatgc
//
