LOCUS       Exported                4522 bp ds-DNA     circular SYN 02-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 2, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          106..2293
                     /organism="Escherichia coli BL21(DE3)"
                     /strain="BL21(DE3)"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:469008"
     CDS             244..1167
                     /codon_start=1
                     /locus_tag="B21_RS04425"
                     /old_locus_tag="B21_00838"
                     /product="hypothetical protein"
                     /label=Nuc_deoxy
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_001034589.1"
                     /translation="MNKKFTDEQQQQLIGHLTKKGFYRGANIKITIFLCGGDVANHQSW
                     RHQLSQFLAKFSDVDIFYPEDLFDDLLAGQGQHSLLSLENILAEAVDVIILFPESPGSF
                     TELGAFSNNENLRRKLICIQDAKFKSKRSFINYGPVRLLRKFNSKSVLRCSSNELKEMC
                     DSSIDVARKLRLYKKLMASIKKVRKENKVSKDIGNILYAERFLLPCIYLLDSVNYRTLC
                     ELAFKAIKQDDVLSKIIVRSVVSRLINERKILQMTDGYQVTALGASYVRSVFDRKTLDR
                     LRLEIMNFENRRKSTFNYDKIPYAHP"
     misc_feature    559..561
                     /label=Conserved E
     misc_feature    1154..1235
                     /label=msr
     misc_feature    1167
                     /label=Branching G
     misc_feature    complement(1225..1310)
                     /label=msd
     CDS             1330..2292
                     /codon_start=1
                     /locus_tag="B21_RS04430"
                     /old_locus_tag="B21_00839"
                     /product="RNA-directed DNA polymerase"
                     /label=RT
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_001320043.1"
                     /translation="MKSAEYLNTFRLRNLGLPVMNNLHDMSKATRISVETLRLLIYTAD
                     FRYRIYTVEKKGPEKRMRTIYQPSRELKALQGWVLRNILDKLSSSPFSIGFEKHQSILN
                     NATPHIGANFILNIDLEDFFPSLTANKVFGVFHSLGYNRLISSVLTKICCYKNLLPQGA
                     PSSPKLANLICSKLDYRIQGYAGSRGLIYTRYADDLTLSAQSMKKVVKARDFLFSIIPS
                     EGLVINSKKTCISGPRSQRKVTGLVISQEKVGIGREKYKEIRAKIHHIFCGKSSEIEHV
                     RGWLSFILSVDSKSHRRLITYISKLEKKYGKNPLNKAKT"
     misc_feature    join(1912..1914,1918..1923)
                     /label=(Y/F)xDD
     terminator      2302..2337
                     /label=T7 Terminator
     CDS             complement(2569..3228)
                     /codon_start=1
                     /label=CAT/CamR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3229..3331)
                     /label=cat Promoter
     misc_feature    3590..4502
                     /label=p15A_origin
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagctca tgttatgcat
      121 gtgcatgaaa accactgcat aaagcgggca ggcgtggcgg ggatacgagc gcgcgccatg
      181 tggtatggag attggatcta ttcataactt gatgtataaa gtagaaaaaa aagcggggag
      241 attatgaata aaaaatttac cgatgagcag caacaacagc ttataggaca tctcacaaag
      301 aaaggcttct atcgaggagc taatattaaa ataaccattt ttctatgtgg tggtgacgtt
      361 gctaatcatc aatcttggcg tcatcaatta tcacaatttt tagcaaagtt cagtgatgtt
      421 gatatatttt atccagaaga tctatttgat gatcttttgg ctggtcaagg gcagcatagc
      481 cttttaagtt tagaaaatat tctggctgaa gctgtcgatg taataatttt atttcctgaa
      541 agtccggggt ctttcacaga gcttggtgcg ttctctaata atgaaaactt aaggagaaag
      601 ttgatttgca ttcaagatgc aaaatttaaa tcaaaacgta gctttattaa ctatggtcct
      661 gttcgcctgt tgcgtaagtt taattcaaaa tctgttttgc gttgtagttc aaatgaacta
      721 aaagaaatgt gtgattcatc tattgatgtt gccagaaaat tacgattata taaaaaatta
      781 atggcatcta ttaagaaggt taggaaagaa aataaagtat caaaagatat tggaaatata
      841 ttatacgcag agcggtttct attgccttgt atctatttac tggatagtgt caactaccgc
      901 acactgtgtg aactagcttt taaagcgata aagcaagatg atgttttatc taaaattatt
      961 gttagatccg ttgtttctcg tctaataaat gaacgaaaaa tacttcaaat gactgatggt
     1021 tatcaggtca ctgctttggg ggctagctat gttaggagcg tctttgatag aaagacactt
     1081 gaccgattgc ggcttgagat tatgaatttt gaaaaccgta gaaaatcaac atttaactat
     1141 gataagattc cgtatgcgca cccttagcga gaggtttatc attaaggtca acctctggat
     1201 gttgtttcgg catcctgcat tgaatctgag ttactgtctg ttttccttgt tggaacggag
     1261 agcatcgcct gatgctctcc gagccaacca ggaaacccgt tttttctgac gtaagggtgc
     1321 gcaactttca tgaaatccgc tgaatatttg aacactttta gattgagaaa tctcggccta
     1381 cctgtcatga acaatttgca tgacatgtct aaggcgactc gcatatctgt tgaaacactt
     1441 cggttgttaa tctatacagc tgattttcgc tataggatct acactgtaga aaagaaaggc
     1501 ccagagaaga gaatgagaac catttaccaa ccttctcgag aacttaaagc cttacaagga
     1561 tgggttctac gtaacatttt agataaactg tcgtcatctc ctttttctat tggatttgaa
     1621 aagcaccaat ctattttgaa taatgctacc ccgcatattg gggcaaactt tatactgaat
     1681 attgatttgg aggatttttt cccaagttta actgctaaca aagtttttgg agtgttccat
     1741 tctcttggtt ataatcgact aatatcttca gttttgacaa aaatatgttg ttataaaaat
     1801 ctgctaccac aaggtgctcc atcatcacct aaattagcta atctaatatg ttctaaactt
     1861 gattatcgta ttcagggtta tgcaggtagt cggggcttga tatatacgag atatgccgat
     1921 gatctcacct tatctgcaca gtctatgaaa aaggttgtta aagcacgtga ttttttattt
     1981 tctataatcc caagtgaagg attggttatt aactcaaaaa aaacttgtat tagtgggcct
     2041 cgtagtcaga ggaaagttac aggtttagtt atttcacaag agaaagttgg gataggtaga
     2101 gaaaaatata aagaaattag agcaaagata catcatatat tttgcggtaa gtcttctgag
     2161 atagaacacg ttaggggatg gttgtcattt attttaagtg tggattcaaa aagccatagg
     2221 agattaataa cttatattag caaattagaa aaaaaatatg gaaagaaccc tttaaataaa
     2281 gcgaagacct aatcgcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     2341 ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct tccggtagtc
     2401 aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg
     2461 acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc
     2521 aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc
     2581 ctgccactca tcgcaatact gttgtaattc atttaacatt ctgccgacat ggaagccatc
     2641 acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     2701 atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     2761 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     2821 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     2881 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     2941 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     3001 cgccatacgg aactctggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     3061 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     3121 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     3181 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     3241 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     3301 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca
     3361 gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt
     3421 cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg
     3481 tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt
     3541 cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctagcgga
     3601 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     3661 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     3721 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     3781 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     3841 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     3901 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3961 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     4021 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     4081 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     4141 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     4201 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     4261 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     4321 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     4381 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat
     4441 ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt
     4501 gtaattctca tgttagtcat gc
//
