LOCUS       Exported                6785 bp ds-DNA     circular SYN 02-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 2, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          106..4556
                     /organism="Klebsiella pneumoniae"
                     /strain="NCTC9143"
                     /mol_type="genomic DNA"
                     /serovar="Capsular Serotype 23"
                     /label=contig: ERS743102SCcontig000003
                     /note="contig: ERS743102SCcontig000003"
                     /db_xref="taxon:573"
     CDS             252..3950
                     /codon_start=1
                     /locus_tag="DX706_RS23405"
                     /old_locus_tag="NCTC9143_04513"
                     /product="Reverse transcriptase (RNA-dependent 
                     DNApolymerase)"
                     /label=drt1a
                     /note="Start codon confirmed (BLASTP)"
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_115196278.1"
                     /translation="MKLLDKKYYNLEPKYEYLKDSFILGLAWKKTDSFVRTHNWYADIL
                     ELDKCAFDISDEVTNWSNEISKNALSKSDIELIPAPKGASWFINQGKWTTNKDNRKIRP
                     LANISIRDQSFATAVTMCLADAIETRQKDCSLSNLGYAEHVKNKVVSYGNRLVCDWDNE
                     RARFRWGGSEYYRKFSSDYRSFLQRPIYIGRETVNKVSGIDDVYIISLDLKNFFGSIKI
                     NLLLEKIKKISADHYAAKFINDNEFWTLANRILSWDWPEESLSLLESLDIKEKNVGLPQ
                     GLASAGALANAYLIEFDESLISKLRTKIEDSQIILHDYCRYVDDIRLVISGEALESNKI
                     KESIHALVQGILDETLAQNPSDNEPYLKINDSKTYILELSDIDNGSGLTNRINEIQHEV
                     GASSIPERNGLDNNIPALQQLLLTEQDNFSEDVDSLFPGFKNDKSIKVESVRRFSAHRL
                     EKSLAKKSKLISPEERKQFDNETSLIAKKLLKAWLKDPSIMVIFRKAIAINPNLDAYST
                     ILEIIFSRIQRNRDKRDKYIMLYLLSDIFRSVIDVYRNLESEYVDDYQKLMGEVTLFAQ
                     KILSCKSFIPNYAYQQALFYLAVINKPFIASNKASFDLARLQCVLIKQHLEPLNSSDGY
                     LFEVSAQISKDYRANAAFLLSHTNSNKVVDLIIEKFAFRGGEFWNAIWKEIVRMQDKDR
                     INEFRWAISKYESKPNSSEHYLSSVISFKENPFRYEHALLKLGVALVELFDDTEKNVWQ
                     PDGKQYSPHEIKVKLEGNSTSWGELWRPNFSISCSIDKKGEPGKDPRYISPEWLANYPQ
                     TQNDEQKIYWVCSVLRSAALGNVDYTQRNDLKLDKAKYDGIHSQFYKRRMGMLHTPESI
                     VGSYGTITDWFASFLQHGLQWPGFSSSYISQEDILSITNIIEFKNCLLERLGYLNKQIC
                     ISSNVPTLPTVVNRPELASNHFRIVTVQQLFPKDTNFHPSDVTLANPDVRWKHREHLAE
                     ICKLTEQTLNAKLKTESREHTSTADLIVFSELAVHPEDEDIVRALAFRTKAIIFSGFVF
                     CEQDGRIVNKARWIIPDSSESGTQWRVRDQGKHHMTSDEVALGIQGYRPSQHIISIEGH
                     PEGPFKLTGAICYDATDIKLAADLRDLTDMFVIAAYNKDVDTFDNMASALQWHMYQHIV
                     ITNTGEYGGSTMQAPYKEKYHKLISHAHGTGQIAISTADIDLAAFRRKLQIYKKTKTQP
                     AGYNRKH"
     misc_feature    join(1215..1217,1221..1226)
                     /label=(Y/F)xDD
     misc_feature    3606..3608
                     /label=Catalytic C (nitrilase)
     CDS             3959..4393
                     /codon_start=1
                     /locus_tag="DX706_RS23410"
                     /old_locus_tag="NCTC9143_04514"
                     /product="hypothetical protein"
                     /label=drt1b
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_040189938.1"
                     /translation="MDTLVKLATIISPLISAGVAIWAILVAKKTISESKEIAKKTIADT
                     AYQAYLQLAMENPQFSKGYSADCRQERDPMYDQYVWYVARMIFCFEKIIEVEVNLKDSS
                     WANTLEKHLKFHSEHFKKTNVVEEALYIPPILDLIRCAAN"
     terminator      4565..4600
                     /label=T7 Terminator
     CDS             complement(4832..5491)
                     /codon_start=1
                     /label=CAT/CamR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(5492..5594)
                     /label=cat Promoter
     misc_feature    5853..6765
                     /label=p15A_origin
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagtcct taaacacgac
      121 aaaacctgtg atacttacca tggattcctc tatgaaggaa aggtagtata gccattttgg
      181 gtgatacata cagtgaatgt cattgctgta gttgaagtga gtaagagcgc ttaagattaa
      241 gttgagagaa aatgaaacta cttgataaaa agtattacaa cctcgagccc aaatatgagt
      301 accttaagga ctcatttatt ttaggactgg catggaaaaa aacagatagt tttgtaagaa
      361 ctcacaattg gtatgcagat attttagagc tggacaagtg tgcgtttgat attagtgatg
      421 aagtcactaa ttggtcaaac gagatctcaa agaacgctct ttccaaaagt gatattgaat
      481 tgataccggc tccaaaagga gcaagctggt tcattaatca aggtaaatgg actaccaata
      541 aagataatag aaagataagg cctttggcta acatatctat tagggatcag tcttttgcta
      601 cagcagtaac aatgtgcctt gctgatgcta tagaaacaag acagaaagac tgttcgttga
      661 gcaatcttgg ctatgctgag catgtaaaga acaaggttgt tagttacgga aataggcttg
      721 tctgcgattg ggacaatgaa agggcaagat ttcgttgggg aggaagtgaa tattatagga
      781 agttctcttc cgattatcga agctttctac aaagacctat ctatataggc agggaaacag
      841 taaataaagt tagcggaatt gatgatgtat atatcatcag tttagatctg aaaaattttt
      901 tcggttctat aaaaataaac cttctgttag aaaaaatcaa aaaaatatcc gctgatcatt
      961 atgcagctaa attcataaat gataatgaat tttggacttt ggcgaatcgg attttaagtt
     1021 gggattggcc tgaagaatct ttatctttac ttgagagttt ggatataaaa gaaaaaaatg
     1081 ttggtcttcc ccagggatta gcttctgctg gtgctctggc gaatgcatat ctcattgagt
     1141 ttgatgaatc tttaatttct aagcttcgta ctaagataga agacagccaa ataatactgc
     1201 atgattattg tcgatatgtc gatgatatta gattagtgat ttcaggagaa gcactagaaa
     1261 gtaataagat taaggaatct attcatgcat tagttcaggg cattcttgat gagacattgg
     1321 ctcaaaatcc gtcagataat gaaccatatt taaaaattaa cgatagcaag acttatattc
     1381 ttgagctttc agacattgac aacggaagtg ggcttacaaa tcgaatcaat gaaattcagc
     1441 atgaagtagg agcttcgagt atcccagagc gtaacggact cgataataat atcccggcac
     1501 ttcaacaatt attactgacc gaacaggata atttttccga ggatgttgat agtttatttc
     1561 ccgggtttaa aaatgataag tcgataaagg tagaatctgt acgtagattt tctgcccata
     1621 ggctggaaaa aagtttggct aaaaaaagca agctaatttc acctgaggag aggaaacaat
     1681 ttgataatga aacctcactg attgcaaaaa aattattaaa agcttggcta aaagatccat
     1741 caattatggt tatcttccgc aaagcgatag ctatcaatcc taatctagat gcttatagca
     1801 ccattcttga aattattttt tcaagaatac aacgcaatcg tgataaacga gataaatata
     1861 taatgctgta tcttctttct gatatatttc gtagcgtcat tgatgtctat cgaaacctag
     1921 aatcagaata cgtcgacgat tatcaaaaat tgatgggtga agttacattg tttgcccaaa
     1981 aaatactttc ctgcaaatct tttattccaa attacgcata tcagcaagca ttattttatc
     2041 tcgcagtgat caataaacca tttatagcta gtaataaagc ttcttttgat cttgcaaggc
     2101 ttcaatgcgt cttaattaaa cagcatttag aaccgttgaa tagtagtgat ggatacctat
     2161 ttgaggtatc tgctcaaatc agtaaagact accgagcaaa tgccgctttt ctactttctc
     2221 atacaaatag taacaaagta gtagacttaa ttatcgaaaa atttgctttc cgaggaggtg
     2281 aattctggaa tgcaatttgg aaagaaattg ttaggatgca agataaagat aggattaacg
     2341 aatttagatg ggccatatca aaatatgagt caaagccaaa tagttcggag cactatcttt
     2401 catcagtgat cagtttcaag gaaaacccat ttagatatga acatgcgctt ctcaagctag
     2461 gtgtagcatt agttgaactc tttgatgata cagagaaaaa cgtatggcaa cctgatggta
     2521 agcagtattc tccacatgaa ataaaagtaa aattagaagg taactcaacc tcatggggtg
     2581 aattatggcg tccaaatttt agtatttcat gctcgataga taagaaaggt gaacctggta
     2641 aagacccacg ctatataagc cctgagtggt tggcaaatta tccacagact caaaatgatg
     2701 aacaaaaaat ctattgggtt tgcagtgtgc taagaagtgc tgctttaggc aatgtagatt
     2761 atactcaaag aaatgattta aaacttgata aagctaagta tgatggtatc cattctcagt
     2821 tttacaagcg acgtatggga atgttacata caccagagtc aattgttggt tcatatggaa
     2881 ctataacaga ttggtttgca agttttcttc agcatggatt gcaatggcca ggtttttctt
     2941 cttcgtatat aagccaagaa gatatattgt caattactaa tattattgag tttaaaaact
     3001 gtttattgga acggctaggc tacttaaata agcagatatg tatttcatcg aatgttccaa
     3061 ccttaccgac tgttgtcaac aggcctgaat tagcatctaa ccattttaga attgttacgg
     3121 ttcagcagtt atttcctaag gatactaatt tccatccttc tgacgtgact ttggctaatc
     3181 ccgatgtgcg ctggaagcac agagagcacc ttgcggaaat ctgtaagcta acggagcaaa
     3241 ctttaaatgc aaaacttaaa actgagtcta gggaacatac aagcacagct gatctaatcg
     3301 ttttttctga gttagcagtt cacccagaag atgaagatat agttagagca ctggcattta
     3361 gaaccaaagc catcattttt tccggctttg tcttctgtga acaagatggc cgaatagtta
     3421 acaaagctcg ttggattatt ccagactctt cagagtctgg gacccaatgg cgtgtccgtg
     3481 atcaggggaa acatcatatg accagtgatg aagtggctct tggcattcaa ggatatagac
     3541 catcccaaca tattatttca attgagggtc accctgaggg accatttaaa ttaactggtg
     3601 cgatttgcta cgatgcaaca gatataaagc ttgcggcaga tctgagagat ttgactgaca
     3661 tgtttgtcat tgcagcatac aataaagatg tagacacatt tgataatatg gcttcagcac
     3721 tacaatggca tatgtatcag catattgtta ttacgaatac gggagaatat ggaggctcaa
     3781 ctatgcaagc cccgtacaaa gagaaatatc ataaattgat ttctcatgct catgggactg
     3841 gtcaaatagc aattagtact gctgatatag atttagcagc attcaggcgg aagctacaaa
     3901 tatataaaaa gaccaaaacc cagcctgctg gatacaatag aaaacattaa ggatttttat
     3961 ggatacttta gttaagttag ctacaattat ttctccatta attagtgctg gagtagctat
     4021 ttgggcaatt ttggttgcta aaaaaaccat cagtgaaagc aaagaaattg ccaagaaaac
     4081 catcgctgat acggcctacc aagcatattt gcaattagcc atggagaacc cacaattttc
     4141 gaaaggctac agcgcagatt gtagacagga gcgagaccct atgtatgatc aatatgtttg
     4201 gtacgtggct aggatgatat tctgctttga gaaaatcatc gaggttgaag taaacttaaa
     4261 agatagttct tgggcaaata cgttggaaaa acatttgaag tttcattctg aacattttaa
     4321 gaaaacgaat gttgtcgaag aggctctcta tattccccct attttggatc tcataagatg
     4381 tgcagctaac taataactta tcccaatagg attatattcc acacgataag cccactggaa
     4441 aatgtaacat cccaagatag tttttgggat tgtttcccag tgggcggaaa gtatcatgat
     4501 agttgtcacc cccggtggag ctgcaaagat ttttatgggg tgggtgttac attgcgcgca
     4561 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaacc
     4621 tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca
     4681 gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt
     4741 gctttcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta gcaccaggcg
     4801 tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcaat
     4861 actgttgtaa ttcatttaac attctgccga catggaagcc atcacagacg gcatgatgaa
     4921 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatagtga
     4981 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     5041 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     5101 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     5161 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     5221 aagggtgaac actatcccat atcaccagct caccgtcttt catcgccata cggaactctg
     5281 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     5341 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     5401 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     5461 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     5521 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     5581 ttacgtgccg atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa
     5641 cagggacacc aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg
     5701 cgcaaagtgc gtcgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga
     5761 ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg
     5821 gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc ggagtgtata ctggcttact
     5881 atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc
     5941 accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc
     6001 gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt
     6061 cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt
     6121 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
     6181 cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc
     6241 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     6301 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     6361 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6421 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
     6481 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg
     6541 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac
     6601 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat
     6661 ctcaagaaga tcatcttatt aatcagataa aatatttcta gatttcagtg caatttatct
     6721 cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc tcatgttagt
     6781 catgc
//
