LOCUS       Exported                7329 bp ds-DNA     circular SYN 02-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 2, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          106..5100
                     /organism="Escherichia coli"
                     /host="Homo sapiens"
                     /strain="ECOR12"
                     /mol_type="genomic DNA"
                     /country="Sweden"
                     /db_xref="taxon:562"
                     /submitter_seqid="ECOR12_contig_45_consensus"
     CDS             269..1447
                     /codon_start=1
                     /locus_tag="A8C78_RS19335"
                     /old_locus_tag="A8C78_15155"
                     /product="tyrosine-type recombinase/integrase"
                     /label=Integrase
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_001130502.1"
                     /translation="MPLNDMQIRRAKPEDKPYTLGDGQGLSLLIEPNGSKSWRFR*R*A
                     GKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSDVRKEQKLALQAESENAFEKIA
                     REWHQLKSAKWSAGYASDIMEAFKNDIFPYVGTRPVSEIKPLELLNVLRKIEKRGALEK
                     MRKVRQRCSEVFRYAIATGRAEYNPAADLSSALEVHQSNHFPFLKADEIPEFLRALESY
                     TGSKLVQIATKLLMITGVRTIELRAALWQEFDLDNAIWEIPAERMKMRRPHLVPLSTQA
                     LDLLHELKIMTGNYRYVFPGRNDPNKPMSEASINQVIKRIGYEGRLTGHGFRHMLSTIL
                     HEEGFQSAFIEVQLAHVDRNNIRGTYNHAIYLMERQKMMQWYSDYLRKKKGL"
     misc_feature    join(392..394,398..400)
                     /label=Y42X/Y44X stop codons
     gene            1449..2645
                     /locus_tag="A8C78_RS19330"
                     /old_locus_tag="A8C78_15150"
                     /label=A8C78_RS19330
     CDS             1449..2645
                     /codon_start=1
                     /locus_tag="A8C78_RS19330"
                     /old_locus_tag="A8C78_15150"
                     /product="RNA-directed DNA polymerase"
                     /label=drt3a
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:HMM"
                     /protein_id="WP_087902017.1"
                     /translation="MLNQSFSVSNLIKLLKKTDPKRYKIGRNSAEYKKYIADKVNGSIE
                     TYSFGSISNSRINNKNVYIFKDFMDVLVARKINDNIKRVYSVKQNNRHDIIKKVNTVLS
                     EPVNYYIYRLDIKSFYESIDKNIVFQRINNNPIISHNTKKFINGLFKHNAFSANNGLPR
                     GMGLSATLSEIFMEEFDAELARLPEVFYASRYVDDIIVFSFYKIPDYKNYFSRILPNGL
                     HLNERKCSEYTIEDTSTKHSEIEFLGYSFIIHHGLKNQRRHVVIRISEEKIKKIKRRIA
                     LAVKDYSNNSDAELLKKRIKYLTGNILVNSNSNKTDALYSGIYYNYQHLTDKTQLKELD
                     IFKNRMLFSSKGEVGRKILAAGHNLLTAPKKYSFLAGFEKRLLSSFKREDIIKINKVW"
     misc_feature    join(2031..2033,2037..2042)
                     /label=(Y/F)xDD
     CDS             2649..4652
                     /codon_start=1
                     /locus_tag="A8C78_RS19325"
                     /old_locus_tag="A8C78_15145"
                     /product="RNA-directed DNA polymerase"
                     /label=drt3b
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_062891751.1"
                     /translation="MKIKISKSDYKRVLLTDILPYEVPILFSNEGFYKLISENKVLPGT
                     FSEGLKLDSYTIPYSYKIKKGLASSRSLGIIHPSTQLRICDFYDKYEHLMVHMCTKSPF
                     SLRYPSKIGSYYYEKDFLKSRINLKDGLVQFHNHGFDSQETSSSSHFSYKKYPFIYKFY
                     ESYEFHRLERKFRKLLKLDIAKCFSHIYTHSVSWAVKSKEFSKVNRTYNSFEGCLDKLF
                     QDANYGETNGIIIGPEFSRIFAEIILQRVDLNVESHLNLEPGIVKDKSYAIRRYVDDYF
                     IFADDDETFKLIEFVLANELEKYKLYLNESKKEFIERPFVTGATMAKNDIAEIIEDLYG
                     SLIHTEKLDELTAMVNLNPDVKIQPENMNDLFPLKGVWNKKLHADKFIKRIKIAVRKNN
                     TTFDLVSSYLLSAIKSKFFKVIRLLRMFDLSGKEDITYKFFSIFNEVIFFIYAMDFRVR
                     QTYIISQVILEINSFANKQASDISEVIKKNTFDELLMCMKSMGNIHERPVELSNLLICM
                     KGLGEQYKLNPDEFKDLLGISENECFYDLEYFSICSMLHYIGDDVLYLKMKEDIVLAIQ
                     SLISGRNDIKKDTETFMLFLDMMTCPYLTVKHKRIIYRTYVEANTGQKRFTNAVIDSEI
                     DSLKNNVIFFNWSGDADLEHVLYKKELRTAYE"
     misc_feature    join(3474..3476,3480..3485)
                     /label=(Y/F)xDD
     terminator      5109..5144
                     /label=T7 Terminator
     CDS             complement(5376..6035)
                     /codon_start=1
                     /label=CAT/CamR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(6036..6138)
                     /label=cat Promoter
     misc_feature    6397..7309
                     /label=p15A_origin
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcatctca acttccccaa
      121 atgtccgtat tcatccataa ataccctgat ttataacaat tttaccgttt tttagtccat
      181 catcgtccgc agccatccag tagaatccga taaagaatgt gtataggatt gtgtatatgt
      241 tcctgttcgg tcatggattc ctatacacat gcctttaaac gatatgcaga ttcgccgcgc
      301 taagcctgaa gataaaccct atacgcttgg ggatgggcaa ggcttgtcat tgcttataga
      361 acctaatgga agcaagagct ggcggttccg ctaacgctaa gccggtaaac ccaagatgat
      421 ctcgcttggc gtttacccaa cgatcactct tgccgatgct cgttcccgtc gtgatgaagc
      481 tcgaaaactt gtggcagaag gaaagaaccc tagtgatgtt cgaaaagagc aaaagctggc
      541 tctgcaagca gagtcagaga acgccttcga aaagatagcc agagagtggc atcaacttaa
      601 atctgctaaa tggtcggcag gatatgcatc agacatcatg gaagcgttta agaacgacat
      661 ttttccttat gtgggaacaa ggcctgtgag tgagattaaa ccgctagagc tgctgaacgt
      721 actgcgtaaa attgagaaac gtggtgcgtt ggagaaaatg cggaaagtgc gtcagcgttg
      781 ctctgaagtg tttcgctacg caattgcaac gggtagagcg gagtacaatc ctgcggcaga
      841 tctttccagc gctctcgaag tgcaccaatc caatcatttc ccgttcctaa aagctgatga
      901 gatacccgaa tttctgcgtg ccttagagag ttacaccggg agtaagcttg tccagatagc
      961 aacgaaatta ctgatgatta cgggcgtgag aaccatcgaa ttacgcgcgg cattatggca
     1021 agaatttgat ctggataacg ctatttggga aattcctgct gaaaggatga aaatgcgcag
     1081 gccgcatctt gtgccattgt cgacccaagc gttagattta ctccatgaac tcaagataat
     1141 gacagggaac tatcgttatg tttttccagg acggaacgat ccgaacaaac cgatgagcga
     1201 agctagcata aatcaagtta tcaagcgtat cggttacgaa ggccgactca ctggtcacgg
     1261 gttcagacat atgttatcaa caattttgca tgaagaaggt tttcaatcag catttattga
     1321 agtccaatta gctcatgttg atagaaataa tataagagga acttataatc atgccatata
     1381 ccttatggaa aggcagaaga tgatgcaatg gtacagtgat tatcttcgca aaaaaaaggg
     1441 gttataatat gttaaaccag tcattttccg tttcgaactt aattaagctt ttaaaaaaaa
     1501 ccgatccaaa aagatacaaa attggtagga attcagctga atataaaaaa tatatagctg
     1561 ataaagttaa tggctcaatt gaaacatact catttggttc gatctcaaat tcaagaatta
     1621 acaacaaaaa tgtgtatata tttaaagatt ttatggatgt acttgtcgcc aggaaaataa
     1681 atgataacat taagcgtgtg tatagtgtta aacaaaacaa cagacatgac atcataaaaa
     1741 aagtaaatac agtgttaagt gagcctgtaa attattatat ttacaggctg gatattaaga
     1801 gtttttatga atcaatagat aaaaatatcg ttttccaaag aattaataat aacccgatta
     1861 tttctcataa tactaaaaaa tttatcaatg gtctttttaa acataacgct ttctctgcaa
     1921 ataacggact tccccgtggt atgggattaa gtgcgacttt atcagaaata tttatggagg
     1981 aatttgatgc tgagttggcg aggctgcctg aagtatttta tgcttcaaga tatgtggatg
     2041 atatcatagt tttttcattc tataaaatac cagattataa aaattatttt tcaaggattt
     2101 taccaaatgg attacattta aatgaaagaa agtgcagtga gtataccata gaggacactt
     2161 caactaaaca ttctgaaatt gagtttttgg gatattcatt tattatacac catggattaa
     2221 aaaatcagcg tcgtcatgtt gtgatcagaa tttcggagga gaaaataaag aaaataaaaa
     2281 gaaggattgc acttgcggta aaagattact caaataattc tgatgcagaa ctcttgaaga
     2341 aaagaataaa gtatttaact ggtaatatat tagtaaactc caatagtaat aaaactgatg
     2401 ctttatatag tggaatttat tacaattatc aacatttaac tgataaaaca cagctcaagg
     2461 aacttgatat atttaagaat aggatgctat tttcttcaaa gggcgaggtg gggagaaaaa
     2521 ttttagcagc aggtcacaac ttattaactg cgcctaaaaa atactcattt ttggctggtt
     2581 ttgaaaaacg gctactgtct tcttttaaac gggaagatat tattaaaata aataaggttt
     2641 ggtgattcat gaaaattaaa atatcgaaga gtgattataa aagagtactt ctcacggata
     2701 ttttaccata tgaagtccct atcctttttt ctaacgaagg tttctataag ttaatttctg
     2761 aaaataaagt tttacccgga acattttcag aaggccttaa gctggattct tataccatcc
     2821 cttactccta taaaataaaa aaggggctgg cgagttctcg aagccttggc attatacatc
     2881 cttcaacgca gttaagaatc tgtgattttt atgataagta tgaacatttg atggttcata
     2941 tgtgtacaaa aagtccgttt tcgctacgtt atcctagcaa aatagggagc tattattacg
     3001 aaaaggactt cttaaaaagt agaataaatc taaaagatgg tcttgtacaa tttcataatc
     3061 atggctttga ttcccaagaa acttcctcat cttcccattt ttcatataag aaatatcctt
     3121 tcatctataa gttttatgag tcatatgaat ttcatagatt ggaaaggaag tttaggaaac
     3181 ttttaaagct tgatattgct aagtgtttta gtcatatata tacacacagc gtttcatggg
     3241 ctgtaaaatc taaagaattc tctaaggtta atagaactta taacagcttt gaaggttgtt
     3301 tggataagct ttttcaagat gccaattatg gtgaaacaaa tggcataata attgggcctg
     3361 aattttcaag gatatttgcg gagattatat tacagcgcgt tgacttgaat gttgagtctc
     3421 atttgaatct tgagccaggc atagttaaag ataagagcta tgctataaga cgttacgttg
     3481 atgattattt tatatttgcg gatgatgatg aaacatttaa gctaatagaa tttgtactgg
     3541 caaatgaact cgaaaaatat aagctttatt tgaatgaatc taaaaaggaa tttatcgaga
     3601 ggccattcgt gactggagct acgatggcta aaaatgatat tgcagaaatc attgaggatt
     3661 tatatggatc gttaatccat actgagaagt tggatgagtt aacagctatg gttaatttaa
     3721 atccagacgt caaaattcag cctgaaaata tgaatgacct ttttccattg aaaggtgtgt
     3781 ggaataaaaa gctacacgcg gacaaattta taaaacgaat caaaattgcg gttagaaaaa
     3841 acaataccac atttgatctt gttagctcat acttattaag tgcgattaag agtaagtttt
     3901 tcaaagtaat taggctgttg aggatgttcg atctgtcagg aaaagaagat ataacttata
     3961 aattcttctc aatattcaat gaggtgattt tttttattta tgctatggat tttcgagtcc
     4021 gacagacata cataattagc caagttattt tggaaataaa ttcatttgct aataagcaag
     4081 cttcagacat tagtgaagtt ataaaaaaga atacttttga tgagcttctt atgtgcatga
     4141 aaagcatggg taatattcat gagaggccag tggagttatc taacttactt atatgtatga
     4201 aaggtttggg ggagcagtat aaactcaatc cagatgaatt taaggatttg ttgggtatta
     4261 gtgagaatga gtgtttttac gatttagaat atttttctat atgcagcatg ttacactata
     4321 taggcgatga tgttctctat ctaaaaatga aagaagatat tgtccttgct atacagagtt
     4381 tgataagtgg tcggaacgat ataaaaaaag acactgaaac atttatgcta ttccttgata
     4441 tgatgacgtg cccatatctt acagttaagc ataagagaat aatttataga acatatgtcg
     4501 aagcaaatac aggtcaaaaa agatttacga atgcagtaat tgattctgaa attgattctt
     4561 taaaaaataa tgtaatcttt tttaactggt ctggagatgc tgatcttgag cacgttcttt
     4621 ataaaaaaga gttgcgaaca gcatatgaat agtagtattt taatttcgtt aaagggttgc
     4681 gatgcctaag gtttcgacct gaagcagata ccggaagatc ggcttttgaa tgttcatccg
     4741 aaagatattc gcgatacgtt ttgaggatgg accgatttag acacactatt gccttttagc
     4801 taaacaggcc gcgaaagcgg cctttttaat gaatcagatt tcccctcacc gatctcaata
     4861 cttcccctca gcgtgcgcag ccccgcccgc ctgcccgctt cgcttaacag actggttttc
     4921 atgcacccct taaatcgtct cagaagccac cacacaaggg ctttcgcgtc aaaaatggcg
     4981 catgagactc atgcgttttc atgcgccata gatatgcact catacgctct caggccagct
     5041 agggaaaaag cgtaaaaaat cccggtactg gaccgagact tcgtgggcgt attttgctaa
     5101 cgcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     5161 aacctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa
     5221 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga
     5281 atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca
     5341 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
     5401 caatactgtt gtaattcatt taacattctg ccgacatgga agccatcaca gacggcatga
     5461 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccata
     5521 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     5581 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     5641 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     5701 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     5761 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcatcgc catacggaac
     5821 tctggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     5881 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     5941 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     6001 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     6061 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     6121 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
     6181 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
     6241 tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga tttagtgtat gatggtgttt
     6301 ttgaggtgct ccagtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg
     6361 ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc tagcggagtg tatactggct
     6421 tactatgttg gcactgatga gggtgtcagt gaagtgcttc atgtggcagg agaaaaaagg
     6481 ctgcaccggt gcgtcagcag aatatgtgat acaggatata ttccgcttcc tcgctcactg
     6541 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag
     6601 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc
     6661 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     6721 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt
     6781 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     6841 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     6901 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6961 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
     7021 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
     7081 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
     7141 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
     7201 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt
     7261 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
     7321 tagtcatgc
//
