LOCUS       Exported                3942 bp ds-DNA     circular SYN 02-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 2, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          106..1713
                     /organism="Escherichia coli KTE25"
                     /strain="KTE25"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1169344"
                     /submitter_seqid="acEva-supercont1.2"
     CDS             179..1663
                     /codon_start=1
                     /locus_tag="WEI_RS10260"
                     /old_locus_tag="WEI_02686"
                     /product="RNA-directed DNA polymerase"
                     /label=drt5
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_001524904.1"
                     /translation="MTSTIDFYESDFSATLYPLKTNQILLKHHSQEMSEYIYQKVINPA
                     YPTDSFLSQQKVFSTKPKGHLRRTVKLDPVAEYFIYDVIYRNRKIFRPEVSESRKSFGY
                     IFRNGSRIPIHVSYNEYKQSLKKYSELYSHSIHFDIASYFNSLYHHDIIHWFSSKEGVS
                     PADVEALGQFFREINSGRSIDFMPQGIYPAKMIGNEFLKFVDLHGRLKSAQIVRFMDDF
                     TIFDNDIETLNNDFIRIQQLLGQVSLNINPSKTTFDNVMGDVNETLTQIKSSLKEIITE
                     YEHIPTASGVEVVETNIEIIKHLDDEQVNKLIDLLKDEKIEESDADLILGFLRTHNDSL
                     LSQMPMLLGRFPNLIKHIYTICSGITDKSGLVKILLSYLNTNNNFLEYQLFWIGAIVED
                     YLLGVGEYGSVLHKLYELSGDFKIARAKVLEIPEQGFGFKEIRNEYLRTGQSDWLSWSS
                     AIGTRNLKSAERNYILDYFSKGSPINYLVASCVKKL"
     misc_feature    join(830..832,836..841)
                     /label=(Y/F)xDD
     terminator      1722..1757
                     /label=T7 Terminator
     CDS             complement(1989..2648)
                     /codon_start=1
                     /label=CAT/CamR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2649..2751)
                     /label=cat Promoter
     misc_feature    3010..3922
                     /label=p15A_origin
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaaagtg aacggatgta
      121 tattgagtgc aatgtgatta actatctgtt gttacaatat ttagataggt gataaaatat
      181 gacatctacc attgattttt atgaatctga tttctcagcc acattatacc cattaaaaac
      241 caatcaaata ttactcaagc atcactcaca agagatgtca gaatatattt atcagaaggt
      301 cattaatcct gcatatccaa cagatagttt tctgtctcag caaaaagtct tttcgactaa
      361 acctaaaggt catttgagac gaactgtaaa attagatcca gtagctgagt attttattta
      421 tgatgttatc tatcgaaaca ggaagatatt taggccagaa gtaagcgagt cgagaaaaag
      481 ctttggatat atttttagga acggtagcag gatacctatc cacgtttcct ataatgaata
      541 taaacaaagc ttaaaaaaat attctgagct atattctcac agtatacatt ttgacatagc
      601 atcttatttt aatagtttat atcaccatga tataatccac tggtttagct caaaagaagg
      661 agttagccct gcggatgttg aagctctcgg acagtttttt cgcgaaatta actcaggacg
      721 aagtatcgat tttatgcccc aaggaattta tccggcaaaa atgatcggta atgagtttct
      781 aaaattcgtt gatttacatg gtcgcctaaa atctgctcaa atagtaagat ttatggatga
      841 ctttactatt tttgacaatg acattgaaac actaaataat gatttcatca gaatacagca
      901 gttattaggg caagtatcct taaatataaa tccgtcaaaa accacatttg acaatgtgat
      961 gggagatgtg aatgaaacct taactcagat caagtcatca cttaaagaaa tcattacgga
     1021 atatgaacat atacctacag cctcaggggt agaggtagtc gagactaata ttgaaatcat
     1081 aaagcacctt gatgatgaac aagttaacaa attaatagac ttgctaaaag atgaaaaaat
     1141 agaagagtct gatgccgatt taattcttgg ttttttgaga actcataatg atagtttact
     1201 ttctcagatg ccaatgctat taggcagatt cccaaattta ataaaacata tttatacgat
     1261 ctgttcaggt attaccgata aatcaggatt agtaaaaata ttgctcagct atttaaatac
     1321 taataataac tttttagaat atcaattgtt ttggattgga gcaatagttg aagactatct
     1381 attaggtgta ggtgagtatg gctccgtttt acacaagtta tatgagttat ctggtgattt
     1441 taaaattgcc agagcaaaag tattagagat accggaacag ggttttggtt tcaaagaaat
     1501 aaggaatgaa taccttagaa ccggacaatc agattggtta tcatggtctt cggctatcgg
     1561 tacgagaaat cttaaatcag cagagagaaa ctatattctt gattatttct caaaaggctc
     1621 accaataaat tatcttgttg catcttgcgt caagaaactt taatttaaaa gccaccttct
     1681 tgaaaggtgg ctttaaaaaa tacctttagt tcccgcaata actagcataa ccccttgggg
     1741 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
     1801 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     1861 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
     1921 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct
     1981 taaaaaaatt acgccccgcc ctgccactca tcgcaatact gttgtaattc atttaacatt
     2041 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc
     2101 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc
     2161 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     2221 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     2281 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     2341 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     2401 accagctcac cgtctttcat cgccatacgg aactctggat gagcattcat caggcgggca
     2461 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     2521 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     2581 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     2641 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     2701 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     2761 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     2821 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct
     2881 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc
     2941 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc
     3001 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc
     3061 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt
     3121 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc
     3181 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac
     3241 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga
     3301 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
     3361 ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt
     3421 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     3481 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
     3541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac
     3601 cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt
     3661 taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg
     3721 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg
     3781 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat
     3841 cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
     3901 agccccatac gatataagtt gtaattctca tgttagtcat gc
//
