LOCUS       Exported                6663 bp ds-DNA     circular SYN 02-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Linyi Gao
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 2, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          106..4434
                     /organism="Cronobacter sakazakii"
                     /strain="NCTC 8155"
                     /mol_type="genomic DNA"
                     /country="United Kingdom"
                     /isolation_source="milk"
                     /collected_by="Farmer"
                     /collection_date=1980
                     /db_xref="taxon:28141"
                     /culture_collection="NCTC:8155"
     CDS             689..4312
                     /codon_start=1
                     /locus_tag="AFK64_RS15595"
                     /old_locus_tag="AFK64_15625"
                     /product="SIR2 family protein"
                     /label=dsr2
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_015387030.1"
                     /translation="MTNKNKIKPLLNNISARLWDGRAAILIGAGFSRNAKPLTSKARKF
                     PMWNDLGDIFYESVYCKKNDNRYSNVLKLGDEVQAAFGRATLDKLIMDHVPDKEYEPSK
                     LHVSLLSLPWIDVFTTNYDTLLERASVNVDSRKYDIVLNKNDLMNAERPRIIKLHGSFP
                     SERPFIVTEEDYRKYPLENSPFVNTVQQSLIENTLCLIGFSGDDPNFLNWIGWIRDNLG
                     TENSPKIYLIGLFSFNEAQRKLLEKRNISIVDLSFLGDFGKDHYLAHQRFIQFLYESKN
                     RDNLIEWPIETNYDRIVFNDGIELKTEKIKKCILEWAQSRQSYPNWLILPESNRSNLWQ
                     NTIDWLSVANYDVAWDGSDDLDFGYEITWRLNKALLPIFNDTSEFLFKLIEKYEINYVS
                     GINNKIIDFDEKYSHITLSLMRFCRQENLIDKWKNLNDLLIQNLDRLTPEVKSDYYYEN
                     ILFSYFNLNFDEARNKLSNWETNKLLPHHEIKRAGLLAEFGMLDEAINLLEETLSTIRR
                     NSLLSSRNIDYSSESQEAYGIYILRMFKRSLRLDSKDDDYSSEYNSRLATLSQYRSDPE
                     NEIKYLEIKLESLPGTFKNTNDTDFDLNKRTVTTYLGGSPTEVRSLDAFSFFLLAEELG
                     LPFHIPGMNIFSGIVENAARHIYQYSPEWAIFSIFRTFNKDKAKSLFNRNRISSLERKK
                     VEDLFDGYYKKYEQIITKKIEDRLNDKLEIEISTLSIIPEILSRLVTKVSFNKKKDIIH
                     LLLKLFNSDNFHQYMETKDLLKRTISNLSDLQKISLIDIFIDFPSAPPNTQLHMGQRYN
                     FLTPFECLLGVTITPPKENSKKIASAKLKKDINDLKSDNLDLRKAVSQKLITLYNLEML
                     NKSDTTKLIKNLWSKRDNFGFPIGSGYYKFFFINNLNPDNENIADKFISIIKTYKFPVQ
                     EGKRVSITGGLDEYCTELNGALHHISLPEKTLSEIISKIHDWYVKDRAWLEKRDDLAKE
                     FTLRFRNITNIITTILEHHKDKLHAESINEISSLLDKMKEDKIPVNSAVTMLCLKNKST
                     YLERIKDIENGLYSFNKDDVIEAINSTYVFIRNNEFPLTIIQAISDKIAWDRNPRLPDC
                     YNLIAYIINSCEFTLPDYLIEKILRGLAYQINIDDRDFVDNNEYLNHLEKKLSATKLAA
                     SMFRKNETLGIDQPSIIQEWKNMCNSRNEFDEIRNEWNNNI"
     misc_feature    1163..1165
                     /label=Catalytic H (SIR2)
     terminator      4443..4478
                     /label=T7 Terminator
     CDS             complement(4710..5369)
                     /codon_start=1
                     /label=CAT/CamR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(5370..5472)
                     /label=cat Promoter
     misc_feature    5731..6643
                     /label=p15A_origin
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagtaag acaagggttg
      121 agcaggctac taatcgttac acaggctaac aaaggcatat taagacgatt tgtagcgctg
      181 taaccttgaa aattatgtac aagcgccccg cattacgtcg ttttaaaggc catcggattc
      241 aggcccgacg cggcttcacg cgattataac cgtgaaaaat cccccccgca tagaacctga
      301 attatccccg ccgccgcgca gaactgacag cgcttcagaa ccgttaaccc tctcagaaat
      361 cccgcttttt tactgtaaaa aaccatgcat aaggtgcatg gttttgcatg cgtttcaccg
      421 acactgaatc ccccgccagc gccagcagta gcgtgccctg aggccgttaa tgcacccgta
      481 ttaaaagcgc cctgttaagc gagcaggcgg ggcggggcga gcattgcgcg tcggtgttac
      541 caattctata tggacattga gcaattcaaa tataataaag gttgggtata tttcgtcctc
      601 aacgatgtca aaaactgcaa aagcgtatta taattcagat cattttcaga ccacctattt
      661 taatcatgca tgcaaaatgg aatatgtgat gacaaataaa aacaaaatca aaccattatt
      721 aaataatata tccgctcgcc tttgggatgg tcgtgcagct atattgatag gagctgggtt
      781 cagtcggaat gcaaagccat taacaagcaa ggcaagaaag tttccaatgt ggaacgactt
      841 aggtgacatt ttttatgaaa gtgtttactg caaaaaaaac gacaatagat attcaaatgt
      901 attgaagcta ggagatgaag ttcaggctgc atttggtaga gcgacacttg ataaattaat
      961 catggatcat gttccagata aagaatatga accatccaaa ttacatgttt cccttctttc
     1021 cttgccgtgg attgatgttt ttacgactaa ttatgataca ttacttgagc gagcaagtgt
     1081 taatgtcgac tccagaaaat atgacattgt ccttaataaa aatgatttaa tgaatgctga
     1141 aagaccaaga attataaaac tgcatggtag cttcccatca gaaaggccct tcatagttac
     1201 ggaggaagat tacagaaagt atcctttaga aaattctcct tttgtgaata ccgttcaaca
     1261 atcattgatt gagaatactc tatgtctgat aggattttcg ggtgacgatc ctaacttctt
     1321 aaattggatt ggttggataa gagataatct tggcacagaa aattcaccca aaatatactt
     1381 gatcggtctt ttttcattta atgaagcaca acgtaagctt ttagaaaaaa gaaatatttc
     1441 cattgttgat ttaagttttc taggtgattt tggcaaggat cattatctag cacaccaacg
     1501 ctttatccaa ttcttatacg aatcaaaaaa tcgagacaac ctaatagagt ggccaataga
     1561 aaccaattat gacagaattg tttttaatga tggcattgaa ttaaaaactg agaaaattaa
     1621 aaagtgtatc ttagaatggg ctcagtcaag acaatcatac ccgaactggc ttattttgcc
     1681 ggaatcaaac agaagtaatt tatggcaaaa cactatagat tggttatctg ttgctaatta
     1741 tgatgtcgct tgggatggtt ctgatgatct tgattttgga tatgaaatta catggcgact
     1801 aaataaagct ttgctaccaa ttttcaatga tacatcagaa ttcttattta agttgattga
     1861 aaaatatgag atcaattacg tttcggggat aaataataaa atcattgact ttgatgaaaa
     1921 atactctcat ataaccctca gtttaatgag attctgtcga caagaaaacc ttattgataa
     1981 atggaagaat ctaaacgatt tattaattca aaatcttgat cgattaacac cagaggtaaa
     2041 atctgattat tattatgaaa atatattatt ttcatacttc aatttaaact tcgatgaagc
     2101 cagaaacaaa ctctccaact gggaaacgaa taaactcctc ccccatcatg aaataaaaag
     2161 agcaggatta cttgccgaat ttggaatgct tgatgaagca atcaatcttc ttgaagaaac
     2221 tttatctacg attcgaagaa acagtttgct ttcatctaga aacattgact attccagtga
     2281 atctcaagaa gcatatggaa tctatatttt gcgaatgttt aaacggagtt tgcgtttaga
     2341 tagcaaagat gacgattatt catctgagta taactcgcgg ttggctacat tatcacaata
     2401 tcgcagcgat cctgaaaacg aaataaaata cctagaaatt aaactagagt cactaccagg
     2461 taccttcaag aataccaatg acacggattt cgatcttaac aaaagaacgg tgaccactta
     2521 tttaggagga agcccaacag aagtgaggtc attagatgct tttagtttct ttctactggc
     2581 agaggaactt ggcctccctt tccacatacc aggaatgaac atttttagtg gaatagttga
     2641 gaatgcagct cgacatattt atcaatactc tccagagtgg gctatttttt caatatttag
     2701 aacatttaac aaggataagg ccaagagtct attcaatcga aatagaattt cgtctcttga
     2761 gcgaaaaaag gttgaagatt tatttgatgg atactacaaa aaatatgagc aaattatcac
     2821 aaaaaaaata gaagatagat taaacgataa acttgagata gaaatttcta cgctatcaat
     2881 cattcctgaa attctttccc ggctagttac aaaagtatca tttaataaaa agaaagacat
     2941 tattcacctt ttgcttaaac tgtttaactc ggataatttt catcaataca tggagactaa
     3001 agatctatta aagcgcacta tttccaattt gagcgactta caaaagatct cactaataga
     3061 tattttcatt gatttcccct ccgcgcctcc caatacccaa ttacatatgg gtcaaagata
     3121 caacttcctt actccatttg aatgtctatt aggggttaca ataacccccc caaaagaaaa
     3181 ctctaaaaaa atcgcatctg caaaattaaa aaaagatata aacgatttaa aaagtgataa
     3241 tttagacttg aggaaagctg tatcacaaaa gctcataaca ttatataacc tagaaatgct
     3301 taacaaatct gacacgacta aacttataaa aaacctttgg tcaaagcgtg ataactttgg
     3361 attcccaata ggcagtggtt actataaatt tttctttata aacaacctta acccagataa
     3421 tgaaaatata gccgacaaat tcatttctat aattaaaaca tacaaatttc ctgtgcaaga
     3481 aggaaaaaga gttagtatta caggtgggtt agatgagtat tgtactgaac tcaatggagc
     3541 gctacaccat ataagtcttc cagagaaaac cctatctgaa ataatttcaa aaatacatga
     3601 ctggtatgtc aaggatcggg cctggcttga aaaaagagat gatttagcca aggagttcac
     3661 tcttagattc agaaatatca caaatatcat aacgacaatt ttagaacacc ataaggacaa
     3721 attacatgct gaatctataa atgaaatatc aagcctacta gataaaatga aagaagacaa
     3781 gatacctgta aactcagcag taacaatgct ttgtctgaaa aataaaagca cttacctcga
     3841 gagaataaaa gatatagaga atggactata tagctttaat aaagatgatg ttattgaagc
     3901 tatcaactca acttatgtct ttattagaaa caatgaattt ccactaacca tcattcaagc
     3961 tatcagcgat aaaatcgcat gggatagaaa ccctcgcctt cctgattgct acaatttaat
     4021 tgcatatata attaactcgt gtgaatttac tcttccagat tatttaatag agaaaatcct
     4081 tcgagggctg gcatatcaaa taaacattga tgatagagat tttgttgata acaatgaata
     4141 tttgaatcac cttgagaaaa aacttagtgc aacaaagctg gctgcttcta tgtttagaaa
     4201 aaatgaaaca ctaggtattg accaaccttc tatcattcaa gagtggaaaa acatgtgcaa
     4261 ctctagaaat gagttcgatg aaattaggaa tgaatggaac aacaatatat aaataaagga
     4321 agaacaccca atttatattg ggtgttctgt tcacgaaacc cttttaccat aatcgaatgg
     4381 caatataaat tgagattgaa atttattctc atctaattaa tcagcccacc attgcgcaat
     4441 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaacctc
     4501 aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc
     4561 aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc
     4621 tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt
     4681 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcaatac
     4741 tgttgtaatt catttaacat tctgccgaca tggaagccat cacagacggc atgatgaacc
     4801 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catagtgaaa
     4861 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     4921 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     4981 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     5041 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     5101 gggtgaacac tatcccatat caccagctca ccgtctttca tcgccatacg gaactctgga
     5161 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     5221 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     5281 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     5341 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     5401 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     5461 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     5521 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     5581 caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
     5641 tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt
     5701 gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat
     5761 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac
     5821 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
     5881 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
     5941 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
     6001 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     6061 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
     6121 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     6181 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     6241 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6301 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
     6361 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
     6421 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
     6481 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
     6541 caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct
     6601 tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca
     6661 tgc
//
