LOCUS       Exported                6749 bp ds-DNA     circular SYN 02-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Linyi Gao
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 2, 2020 from SnapGene 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          106..4520
                     /organism="Escherichia coli"
                     /host="dog"
                     /strain="ECOR25"
                     /mol_type="genomic DNA"
                     /country="USA: NY"
                     /db_xref="taxon:562"
     CDS             528..4349
                     /codon_start=1
                     /locus_tag="A8F89_RS00635"
                     /old_locus_tag="A8F89_22730"
                     /product="hypothetical protein"
                     /label=tmn
                     /note="Derived by automated computational analysis 
                     usinggene prediction method: Protein Homology."
                     /inference="COORDINATES:same species:RefSeq"
                     /protein_id="WP_001683567.1"
                     /translation="MIMSTPWLTPIVADSDHAEANAVSYEALTPTELDSDKAGCYISAL
                     NYAYEHPDIRNIAVTGPYGAGKSSVLKTWCKAHNGTLRVLTVSLADFDMQRHVDESNGD
                     SSSDEGTKNTGSVEKSIEYSILQQILYKNKKHELPCSRIDRISDVTAGQILRSASFLTG
                     TILLSGAALFFLAPDYVTTKLSLPGAFARYLLECPFGVRVSGAVASVMGSLCLLLNQLH
                     RIGIFDRKVSLDKVDLLKGAVTTRASSPSLLNVYIDEIVYFFDSTKYDVVIFEDLDRFN
                     NGRIFVKLREINQIINNCLSDRKPVKFIYAVRDGIFNSAESRTKFFDFVMPVIPVMDNQ
                     NAYEHFVKKFKEEEINNNLSECISRIATFIPNMRVMHNITNEFRLYQNLVNSRENLAKL
                     LAMIAYKNLCAEDYHGIDSKKGVLYHFIQSYLDHEIQNELLHSANNELEDMAQSLVAIT
                     NEKLANRENLREELLMPYLSKNYSGALVFYTEGRQISLDDLIQDEDEFLMLLDKENIQV
                     VTPYNRQNFLMINQRDTEKLKQQYEKRCHLIETKSVDNITRVKNNISSLESLRTEILSG
                     TVADIAEKMTNEGFVAWIKKKEDTGVLTIQSEHEQIDFIFFLLSSGYLSTDYMSYRSIF
                     IPGGLSETDNLFLKDVMSGKGPEKTFSFHLDNVNNIVERLKKLGVLQRDNAQHPAVIRW
                     LIDNDPDTLKNNIMALLSQTGSQRVVSLLMLMQNDFTTYVRLRYLEIFMSDEHILNRLL
                     AHLCASEERTPEQKFFVQEIAAHLLCLTEKSNIWQSVEINKRIGELIDSSPILITAVPK
                     GYGDAFFEVLKDNTLSVSYIPGDVGDEKCSVIRKIAGAGLFKYSVSNLKNVYLCLTQDK
                     NEERMSFSLYPFHCLESLAISELTEILWTNIEDFILSVFIESEEIDRIPELLNSSEVSM
                     TVVEQIIAKMDFCINNLDDIINRSECADNNASGRNIYSMLLQHDRIFPSFDNIIHLLHD
                     TSINTSGELVQWVNEKHFEFEPSDIVINDTGIFNNFISELICSPVISEEALLKVLSNLN
                     VVIIDVPENIPLRNAELLCSEKKLAPTVNVFTVLFNALSENVDDINRMNTLLGNLIAQR
                     PEIITQEPEDIFYIEGDFDEELASELFRHKLIGMNIKVAALRWLRDNKPGILDKSYLLS
                     LDILAELSPWMGDDDLRLTLLKRCLVAGDAGKDALCVVLNSFADESYHGLLPHDRFRKI
                     PHSVDLWEVAELISNLGFIQPPKMGSGRDEHKIVITPVRYVRDVEFYD"
     misc_feature    join(708..710,723..731)
                     /label=Walker A
     misc_feature    1005..1058
                     /label=Transmembrane
     misc_feature    1122..1181
                     /label=Transmembrane
     misc_feature    1338..1352
                     /label=Walker B
     terminator      4529..4564
                     /label=T7 Terminator
     CDS             complement(4796..5455)
                     /codon_start=1
                     /label=CAT/CamR
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(5456..5558)
                     /label=cat Promoter
     misc_feature    5817..6729
                     /label=p15A_origin
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaaccgt gctggcatgt
      121 ttttacggag tgacgctttc attaacctgt acacgaactt ctattccggc atcatgacag
      181 gcctgcagcc actgcgccac ttccagcgga tcgccctccc ggcgtaccac tctgccttct
      241 ttattccata actgcagaca ggtgctgccg tcgagacgca ccacaaaatc cccacggcag
      301 gcctgatagg ggtttgaggg ccaaccgtac gaaaacgtac ggtaagagga aaattatcgt
      361 cttaaaaatc gatttatgct atcacagtcg tctcttcagg taagtacggt tgcctttgcc
      421 tgctttcttc tcgtctggtt aagttaagaa attcagagat ccatgcttga gataaaagcg
      481 gaataaaacc agtaaaatgt aactaaaaca acaacggaat tgtatcaatg ataatgtcca
      541 caccgtggct gacaccgatc gttgccgata gtgatcatgc tgaggcaaat gcagtgagct
      601 atgaagcact gactccgaca gaactcgact cagataaagc aggctgttat atcagcgcgc
      661 ttaattatgc ttatgaacat ccggatatcc ggaatattgc tgttaccggg ccgtatgggg
      721 cagggaaaag ctcagtatta aaaacatggt gcaaagctca caatgggaca ctgcgggtgt
      781 taaccgtttc tcttgctgat tttgatatgc agagacatgt ggatgaaagt aatggggaca
      841 gcagtagtga cgaagggacg aaaaatactg gtagtgttga aaaatctatt gaatacagta
      901 ttctgcaaca aatactctac aaaaataaaa agcatgagct tccctgttcc cgcattgacc
      961 gtatatcaga tgtgactgcg ggacaaatat tgcggtctgc gtcttttctg acaggaacca
     1021 ttttactgag tggagctgct ttatttttcc ttgcgccgga ttacgttaca acaaagctat
     1081 ctttgccggg agcattcgcc cgttaccttc ttgaatgccc gtttggggtg cgtgtgtccg
     1141 gtgcagtggc atctgtgatg ggatcgttat gcctgctttt gaaccagtta catcgtatcg
     1201 gtatatttga caggaaagta agtcttgata aagtggacct tctgaaaggc gctgttacaa
     1261 cccgggcatc atcaccttct ttacttaatg tctatattga tgaaattgtc tatttttttg
     1321 attcgactaa atatgatgta gtgatattcg aagatcttga ccgttttaac aatggccgga
     1381 ttttcgtgaa attgcgggaa atcaatcaaa ttattaataa ctgcctttct gacagaaaac
     1441 ctgtaaaatt tatttatgct gtcagagatg gtattttcaa ctcagcagag tcaagaacga
     1501 aattctttga ttttgttatg cctgttattc cagtgatgga taaccagaat gcttatgagc
     1561 attttgttaa aaaattcaaa gaagaagaga taaataataa cttaagcgaa tgtatttctc
     1621 gtattgcgac atttattccc aatatgcgtg taatgcataa tattacaaat gagtttcgac
     1681 tctatcagaa tttagtcaat agtcgggaaa atctggccaa actacttgcc atgatagcat
     1741 ataaaaatct ctgtgcggaa gattatcatg gtatagatag taaaaaaggt gttctttatc
     1801 attttattca aagctactta gaccatgaaa ttcagaatga attattacat tctgcaaata
     1861 acgaacttga ggatatggca cagtcacttg tagcgataac aaatgaaaaa ctcgcaaacc
     1921 gggaaaatct gcgcgaagaa ctgctcatgc cttaccttag taaaaattat agcggcgcgc
     1981 ttgtttttta tacagaagga aggcaaataa gtcttgatga tttgatacaa gatgaagatg
     2041 aatttctcat gcttttagat aaggaaaata ttcaggtcgt taccccctat aacagacaaa
     2101 attttctcat gataaatcag cgggatacag aaaaactgaa gcagcagtat gaaaaacgat
     2161 gccatttaat tgaaactaaa tctgttgata atataaccag agtgaaaaat aatatttcca
     2221 gtctggagtc attgaggacc gaaattcttt ccggaactgt agctgatata gcagaaaaga
     2281 tgacaaatga aggctttgtt gcctggataa agaagaaaga ggatacaggt gtcctgacga
     2341 ttcagtcgga acatgaacag attgatttta tattttttct gttatcaagt ggttatttat
     2401 caacagatta catgtcctat cgctcaatct tcattcccgg agggctgagt gagacagata
     2461 atttatttct taaggatgtt atgtctggta aaggtccgga aaaaacattc tcattccatc
     2521 ttgataacgt taataatatt gttgaacgac tcaaaaagct gggggttctg cagcgtgaca
     2581 atgctcaaca tcctgctgtt atcagatggc tgattgataa tgaccctgat accctgaaaa
     2641 acaatataat ggcattactg agtcagacgg gtagccagcg tgtggttagt ttgctgatgt
     2701 tgatgcagaa cgatttcaca acgtatgttc gcctgcgtta cctggagatt tttatgtcag
     2761 atgaacatat actgaacaga ttgctggcac atttatgtgc gtcagaagaa cgcacacccg
     2821 agcaaaagtt ttttgttcag gaaatagcgg cacacctgtt atgcctgact gaaaaatcaa
     2881 atatctggca atcggttgag attaataaac gtatcggtga gcttatagat tcctccccaa
     2941 ttcttattac tgctgtgcca aaaggatatg gtgatgcgtt ttttgaagtg ttgaaagata
     3001 atacactttc agtttcatat attccaggtg atgtgggaga cgagaagtgt tctgttatca
     3061 ggaaaattgc gggtgcagga ttattcaaat attccgtcag taatcttaaa aatgtttatc
     3121 tttgcctgac gcaagacaag aatgaagaaa gaatgtcatt ctctctttat ccgtttcatt
     3181 gtctcgagtc cctggctatt tctgaattaa cagaaattct gtggactaac atagaagatt
     3241 ttattttatc ggtatttatt gaatcggaag agattgatcg tattcctgaa ttgctgaatt
     3301 cttctgaagt ctcaatgact gttgttgaac agattatagc caaaatggat ttttgtataa
     3361 ataatctgga tgatattatt aatcgttcag agtgtgcgga caataatgct tcagggagaa
     3421 atatctatag catgctgttg cagcatgaca ggatttttcc atcctttgat aatattattc
     3481 atttattgca tgatacatca attaatactt ccggtgaact tgttcagtgg gtaaatgaga
     3541 aacactttga atttgaacca tctgatatag tcataaatga tacaggaata tttaataatt
     3601 ttatttctga attaatttgc tcgccagtca tttcagaaga agctttactg aaagtactga
     3661 gtaatttaaa cgttgttatt atcgatgtgc ctgaaaacat tccattgcga aatgctgaac
     3721 tgttatgttc agagaaaaaa ctggcaccga cagttaatgt ctttacggtg ttgtttaatg
     3781 ctctcagtga aaatgttgat gatattaaca ggatgaatac tctgcttggt aaccttattg
     3841 cccagcgtcc tgagattatt acccaggagc cagaagatat tttttatatc gagggtgact
     3901 ttgatgaaga actggcaagc gaactttttc gtcacaagct aatcggtatg aatataaaag
     3961 ttgccgcttt acgctggttg cgtgataaca aaccgggaat tcttgataag agctacctgc
     4021 tgtcattaga tattctggca gaactgagtc cctggatggg tgacgatgat ctgcgcctga
     4081 cactgcttaa acgttgtctg gttgccgggg atgctggcaa agacgcgctt tgcgtggtgc
     4141 tgaacagttt tgctgatgag agctatcatg gactgttacc acatgacagg ttcaggaaaa
     4201 tccctcactc cgtggatttg tgggaagtgg ccgaattaat cagcaatctt ggatttattc
     4261 agccgccaaa aatggggtca gggcgtgatg aacacaaaat tgttattact cccgtacgct
     4321 atgtccgtga tgttgagttt tatgactgag catcattgat acggtgtttt aattgcctta
     4381 aatacaaaaa taaaaacaga ttaatgctta atgtgcatta atctgtttta gttatcaatg
     4441 gctgttaatt attgttaatt ttacattaat ctttcttttt cttcaggaag atccgaaaac
     4501 tcctggtcac ggatcttcct cgcaataact agcataaccc cttggggcct ctaaacgggt
     4561 cttgaggggt tttttgctga aacctcaggc atttgagaag cacacggtca cactgcttcc
     4621 ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc
     4681 tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca
     4741 cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg
     4801 ccccgccctg ccactcatcg caatactgtt gtaattcatt taacattctg ccgacatgga
     4861 agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     4921 gcgtataata tttgcccata gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     4981 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     5041 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     5101 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     5161 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     5221 ctttcatcgc catacggaac tctggatgag cattcatcag gcgggcaaga atgtgaataa
     5281 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     5341 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     5401 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     5461 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     5521 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt
     5581 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
     5641 cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga
     5701 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc
     5761 tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc
     5821 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc
     5881 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata
     5941 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat
     6001 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt
     6061 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
     6121 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6181 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     6241 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     6301 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
     6361 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc
     6421 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg
     6481 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg
     6541 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
     6601 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt
     6661 tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat
     6721 ataagttgta attctcatgt tagtcatgc
//
