LOCUS       pDS05                  10082 bp ds-DNA     circular     03-JUL-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..738
                     /label="EmR "
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_signal     2557..2572
                     /label="motif 1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(8057..8755)
                     /label="repA (Ts)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    6851..6939
                     /label="ter rrnB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    7753..7845
                     /label="sgRNA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2557..2639
                     /label="PczcD"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    6954..7008
                     /label="P3"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             2703..6809
                     /label="wtCas9sp"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     -35_signal      1589..1594
                     /label="-35\Ap"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6954..7008
                     /label="P3"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(781..1519)
                     /label="CloDF13 ori"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    7753..7845
                     /label="sgRNA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(7871..7912)
                     /label="term"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_signal     2614..2619
                     /label="-10"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_signal     2625..2639
                     /label="motif 2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2474..2516
                     /label="ter rpsD"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_signal     2584..2589
                     /label="-35"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2703..6794
                     /label="M28_Spy0748"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1659..2447
                     /label="aadA (spec)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     -10_signal      1611..1617
                     /label="-10\Ap"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    7008..7745
                     /label="GFP"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(781..1519)
                     /label="CloDF13 ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(7923..7947)
                     /label="gRNARseq"
                     /note="sequence: AACAATAGATTTATTGAGAGGAGGG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          8644..8663
                     /label="repA Ts seqF"
                     /note="sequence: TGACAGCCATAGATACGCCC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(7829..7855)
                     /label="gRNA R"
                     /note="sequence: CTCTCACACCTGCTCACATTGAGACAGAAAAAAAGCACCGACTC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1655..1676
                     /label="aadAseqF"
                     /note="sequence: GAGTATGAGGGAAGCGGtgatc"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          425..444
                     /label="eryR-qFor"
                     /note="sequence: GGTTGCTCTTGCACACTCAA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          7742..7766
                     /label="lin pGh F"
                     /note="sequence: GTAAGGTCTCGGTTTAAGAGCTATG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(7721..7762)
                     /label="OVL1015_BsaI-GFP-R"
                     /note="sequence: GCTCTTAAACCGAGACcTTACTTATAAAGCTCATCCATGCCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2962..2985)
                     /label="dCas9_Sequencing_Down"
                     /note="sequence: CATCTACTTTCGCCATCTCATTTG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(732..758)
                     /label="pGh R"
                     /note="sequence: CTCTCACACCTGCCTGTTTCAAAAGCGACTCATAGAATTATTTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          6940..6965
                     /label="gRNA F"
                     /note="sequence: CTCTCACACCTGCTCACGCGTATAAGAGACAGCCATTCTACAG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(6914..6939)
                     /label="73cas9R2"
                     /note="sequence: AGATGGCACCTGCCAGAACGCTAAATACGCTTCACAGTTTCTTCTTC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          6473..6496
                     /label="dCas9_Sequencing_Up"
                     /note="sequence: GTTTGTGGAGCAGCATAAGCATTA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          7851..7880
                     /label="pGh F"
                     /note="sequence: CTCTCACACCTGCCTGTCAATCGCAACATCAAACCAAAATAAAAAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(7715..7747)
                     /label="LK247"
                     /note="sequence: GCGCGGGATCCTTACTTATAAAGCTCATCCATGCCGTGAGTG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          6127..6146
                     /label="LK292"
                     /note="sequence: CAGTCCTAGTGGTTGCTAAG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          2660..2684
                     /label="OVL205_lacZ up F"
                     /note="sequence: TTCCAATCCCGGGTAAAGCTTCCAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(2444..2472)
                     /label="pCDF-1b R"
                     /note="sequence: CTCTCACACCTGCCGTATACTTGAACGAATTGTTAGACATTATTTG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          2473..2499
                     /label="73cas9F2"
                     /note="sequence: AGATGGCACCTGCCAGAAGTACAAGCACTTTGGGACGTTCTCCCTTAG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(6914..6965)
                     /label="OLcas9R"
                     /note="sequence: CTGTAGAATGGCTGTCTCTTATACGCTAAATACGCTTCACAGTTTCTTCTTC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(6552..6580)
                     /label="dCas9-Seq-1"
                     /note="sequence: GCACTAAGAACTTTATCTAAATTGGCATC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(7819..7845)
                     /label="LK253"
                     /note="sequence: GCGCGAATTCAAAAAAAGCACCGACTCGGTGCCACTT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(6802..6826)
                     /label="OVL473_cas9-R"
                     /note="sequence: CTAGTCTCGAGGTCGACTTAGTCAC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2719..2740
                     /label="cas9semi-F-mut1"
                     /note="sequence: CAATAGGCTTAGATATCGGCAC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          755..781
                     /label="pCDF-1b F"
                     /note="sequence: CTCTCACACCTGCCGTATGAATCTAGAGCGGTTCAGTAGAAAAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          6914..6965
                     /label="OLgRNAF"
                     /note="sequence: GAAGAAGAAACTGTGAAGCGTATTTAGCGTATAAGAGACAGCCATTCTACAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(2719..2740)
                     /label="cas9semi-R-mut1"
                     /note="sequence: GTGCCGATATCTAAGCCTATTG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(9811..9830)
                     /label="PGh rev seq"
                     /note="sequence: TTTCTTTTGCGCGACTCTAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(6914..6963)
                     /label="OLcas9R"
                     /note="sequence: GTAGAATGGCTGTCTCTTATACGCTAAATACGCTTCACAGTTTCTTCTTC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2468..2499
                     /label="73cas9F2"
                     /note="sequence: AGATGGCACCTGCCAGAAGTACAAGCACTTTGGGACGTTCTCCCTTAG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          3655..3678
                     /label="dCas9-Seq-5"
                     /note="sequence: CAGCTTCAATGATTAAACGCTACG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(5757..5781)
                     /label="dCas9-Seq-2"
                     /note="sequence: GCTCAGACTTAGCAATCATTTTACG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(9505..9534)
                     /label="71 - Spc-R-Gibson"
                     /note="sequence: TGAGACGTTTTAGCGTTTATTTCGTTTAGT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          7032..7051
                     /label="OVL1009_F-msfGFPbs.noATG"
                     /note="sequence: TCAAAAGGAGAAGAGCTGTT"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(522..541)
                     /label="eryR-qRev"
                     /note="sequence: CTGTGGTATGGCGGGTAAGT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(4935..4958)
                     /label="dCas9-Seq-3"
                     /note="sequence: CCCCATTACTTTGACCAATTCATC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          6916..6965
                     /label="OLgRNAF"
                     /note="sequence: AGAAGAAACTGTGAAGCGTATTTAGCGTATAAGAGACAGCCATTCTACAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          732..781
                     /label="OLpCDFF"
                     /note="sequence: GAAATAATTCTATGAGTCGCTTTTGAATCTAGAGCGGTTCAGTAGAAAAG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2962..2985)
                     /label="dCas9_Sequencing_Down"
                     /note="sequence: CATCTACTTTCGCCATCTCATTTG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2480..2499
                     /label="LK207"
                     /note="sequence: GCGCGCCTGAGGTTTGGGACGTTCTCCCTTAG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          732..781
                     /label="OLpCDFF"
                     /note="sequence: GAAATAATTCTATGAGTCGCTTTTGAATCTAGAGCGGTTCAGTAGAAAAG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2872..2891
                     /label="dCas9-Seq-4"
                     /note="sequence: AGACAGCGGAAGCGACTCGT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(6990..7019)
                     /label="lin pGh R"
                     /note="sequence: CCTAGGTCTCATATAGTTATTATACCAGGG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(732..781)
                     /label="OLpGHR"
                     /note="sequence: CTTTTCTACTGAACCGCTCTAGATTCAAAAGCGACTCATAGAATTATTTC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(714..738)
                     /label="86 - Ery-R-Gibson"
                     /note="sequence: TTATTTCCTCCCGTTAAATAATAGA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(7101..7120)
                     /label="OVL1140_"
                     /note="sequence: CCGCGAACAGAGAATTTGTG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(732..781)
                     /label="OLpGHR"
                     /note="sequence: CTTTTCTACTGAACCGCTCTAGATTCAAAAGCGACTCATAGAATTATTTC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(6914..6943)
                     /label="73cas9R2"
                     /note="sequence: AGATGGCACCTGCCAGAACGCTAAATACGCTTCACAGTTTCTTCTTC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 ATGAACAAAA ATATAAAATA TTCTCAAAAC TTTTTAACGA GTGAAAAAGT ACTCAACCAA
       61 ATAATAAAAC AATTGAATTT AAAAGAAACC GATACCGTTT ACGAAATTGG AACAGGTAAA
      121 GGGCATTTAA CGACGAAACT GGCTAAAATA AGTAAACAGG TAACGTCTAT TGAATTAGAC
      181 AGTCATCTAT TCAACTTATC GTCAGAAAAA TTAAAACTGA ATACTCGTGT CACTTTAATT
      241 CACCAAGATA TTCTACAGTT TCAATTCCCT AACAAACAGA GGTATAAAAT TGTTGGGAGT
      301 ATTCCTTACC ATTTAAGCAC ACAAATTATT AAAAAAGTGG TTTTTGAAAG CCATGCGTCT
      361 GACATCTATC TGATTGTTGA AGAAGGATTC TACAAGCGTA CCTTGGATAT TCACCGAACA
      421 CTAGGGTTGC TCTTGCACAC TCAAGTCTCG ATTCAGCAAT TGCTTAAGCT GCCAGCGGAA
      481 TGCTTTCATC CTAAACCAAA AGTAAACAGT GTCTTAATAA AACTTACCCG CCATACCACA
      541 GATGTTCCAG ATAAATATTG GAAGCTATAT ACGTACTTTG TTTCAAAATG GGTCAATCGA
      601 GAATATCGTC AACTGTTTAC TAAAAATCAG TTTCATCAAG CAATGAAACA CGCCAAAGTA
      661 AACAATTTAA GTACCGTTAC TTATGAGCAA GTATTGTCTA TTTTTAATAG TTATCTATTA
      721 TTTAACGGGA GGAAATAATT CTATGAGTCG CTTTTGAATC TAGAGCGGTT CAGTAGAAAA
      781 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTTTTGCC CTGTAAACGA
      841 AAAAACCACC TGGGGAGGTG GTTTGATCGA AGGTTAAGTC AGTTGGGGAA CTGCTTAACC
      901 GTGGTAACTG GCTTTCGCAG AGCACAGCAA CCAAATCTGT CCTTCCAGTG TAGCCGGACT
      961 TTGGCGCACA CTTCAAGAGC AACCGCGTGT TTAGCTAAAC AAATCCTCTG CGAACTCCCA
     1021 GTTACCAATG GCTGCTGCCA GTGGCGTTTT ACCGTGCTTT TCCGGGTTGG ACTCAAGTGA
     1081 ACAGTTACCG GATAAGGCGC AGCAGTCGGG CTGAACGGGG AGTTCTTGCT TACAGCCCAG
     1141 CTTGGAGCGA ACGACCTACA CCGAGCCGAG ATACCAGTGT GTGAGCTATG AGAAAGCGCC
     1201 ACACTTCCCG TAAGGGAGAA AGGCGGAACA GGTATCCGGT AAACGGCAGG GTCGGAACAG
     1261 GAGAGCGCAA GAGGGAGCGA CCCGCCGGAA ACGGTGGGGA TCTTTAAGTC CTGTCGGGTT
     1321 TCGCCCGTAC TGTCAGATTC ATGGTTGAGC CTCACGGCTC CCACAGATGC ACCGGAAAAG
     1381 CGTCTGTTTA TGTGAACTCT GGCAGGAGGG CGGAGCCTAT GGAAAAACGC CACCGGCGCG
     1441 GCCCTGCTGT TTTGCCTCAC ATGTTAGTCC CCTGCTTATC CACGGAATCT GTGGGTAACT
     1501 TTGTATGTGT CCGCAGCGCC CGCCGCAGTC TCACGCCCGG AGCGTAGCGA CCGAGTGAGC
     1561 TAGCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC
     1621 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGGGAAGCG GTGATCGCCG
     1681 AAGTATCGAC TCAACTATCA GAGGTAGTTG GCGTCATCGA GCGCCATCTC GAACCGACGT
     1741 TGCTGGCCGT ACATTTGTAC GGCTCCGCAG TGGATGGCGG CCTGAAGCCA CACAGTGATA
     1801 TTGATTTGCT GGTTACGGTG ACCGTAAGGC TTGATGAAAC AACGCGGCGA GCTTTGATCA
     1861 ACGACCTTTT GGAAACTTCG GCTTCCCCTG GAGAGAGCGA GATTCTCCGC GCTGTAGAAG
     1921 TCACCATTGT TGTGCACGAC GACATCATTC CGTGGCGTTA TCCAGCTAAG CGCGAACTGC
     1981 AATTTGGAGA ATGGCAGCGC AATGACATTC TTGCAGGTAT CTTCGAGCCA GCCACGATCG
     2041 ACATTGATCT GGCTATCTTG CTGACAAAAG CAAGAGAACA TAGCGTTGCC TTGGTAGGTC
     2101 CAGCGGCGGA GGAACTCTTT GATCCGGTTC CTGAACAGGA TCTATTTGAG GCGCTAAATG
     2161 AAACCTTAAC GCTATGGAAC TCGCCGCCCG ACTGGGCTGG CGATGAGCGA AATGTAGTGC
     2221 TTACGTTGTC CCGCATTTGG TACAGCGCAG TAACCGGCAA AATCGCGCCG AAGGATGTCG
     2281 CTGCCGACTG GGCAATGGAG CGCCTGCCGG CCCAGTATCA GCCCGTCATA CTTGAAGCTA
     2341 GACAGGCTTA TCTTGGACAA GAAGAAGATC GCTTGGCCTC GCGCGCAGAT CAGTTGGAAG
     2401 AATTTGTCCA CTACGTGAAA GGCGAGATCA CCAAGGTAGT CGGCAAATAA TGTCTAACAA
     2461 TTCGTTCAAG TACAAGCACT TTGGGACGTT CTCCCTTAGT GCTTTTTTGA TTTCTCATAG
     2521 GCCGGCCTGT TAGTCATATG GACACTTAAG GCAAATTGTT CAGAACTGAA TAAAGCTGAC
     2581 GTTTTGCTTC TATCCTTTCT TTGAGTTTTA GTGGATAATG ATAATGAACA AGGTGTTCAT
     2641 AAATCTATTA TAACAAGAAT TCCAATCCCG GGTAAAGCTT CCAGCGGCCG CAGGAGGCAA
     2701 AAATGGATAA GAAATACTCA ATAGGCTTAG ATATCGGCAC AAATAGCGTC GGATGGGCGG
     2761 TGATCACTGA TGATTATAAG GTTCCGTCTA AAAAGTTCAA GGTTCTGGGA AATACAGACC
     2821 GCCACAGTAT CAAAAAAAAT CTTATAGGGG CTCTTTTATT TGACAGTGGA GAGACAGCGG
     2881 AAGCGACTCG TCTCAAACGG ACAGCTCGTA GAAGGTATAC ACGTCGGAAG AATCGTATTT
     2941 GTTATCTACA GGAGATTTTT TCAAATGAGA TGGCGAAAGT AGATGATAGT TTCTTTCATC
     3001 GACTTGAAGA GTCTTTTTTG GTGGAAGAAG ACAAGAAGCA TGAACGTCAT CCTATTTTTG
     3061 GAAATATAGT AGATGAAGTT GCTTATCATG AGAAATATCC AACTATCTAT CATCTGCGAA
     3121 AAAAATTGGT AGATTCTACT GATAAAGCGG ATTTGCGCTT AATCTATTTG GCCTTAGCGC
     3181 ATATGATTAA GTTTCGTGGT CATTTTTTGA TTGAGGGAGA TTTAAATCCT GATAATAGTG
     3241 ATGTGGACAA ACTATTTATC CAGTTGGTAC AAACCTACAA TCAATTATTT GAAGAAAACC
     3301 CTATTAACGC AAGTGGAGTA GATGCTAAAG CGATTCTTTC TGCACGATTG AGTAAATCAA
     3361 GACGATTAGA AAATCTCATT GCTCAGCTCC CCGGTGAGAA GAAAAATGGC TTATTTGGGA
     3421 ATCTCATTGC TTTGTCATTG GGTTTGACCC CTAATTTTAA ATCAAATTTT GATTTGGCAG
     3481 AAGATGCTAA ATTACAGCTT TCAAAAGATA CTTACGATGA TGATTTAGAT AATTTATTGG
     3541 CGCAAATTGG AGATCAATAT GCTGATTTGT TTTTGGCAGC TAAGAATTTA TCAGATGCTA
     3601 TTTTACTTTC AGATATCCTA AGATTAAATA GTGAAATAAC TAAGGCTCCC CTATCAGCTT
     3661 CAATGATTAA ACGCTACGAT GAACATCATC AAGACTTGAC TCTTTTAAAA GCTTTAGTTC
     3721 GACAACAACT TCCAGAAAAG TATAAAGAAA TCTTTTTTGA TCAATCAAAA AACGGATATG
     3781 CAGGTTATAT TGATGGGGGA GCTAGCCAAG AAGAATTTTA TAAATTTATC AAACCAATTT
     3841 TAGAAAAAAT GGATGGTACT GAGGAATTAT TGGCGAAACT AAATCGTGAA GATTTGCTGC
     3901 GCAAGCAACG GACCTTTGAC AACGGCTCTA TTCCCCATCA AATTCACTTG GGTGAGCTGC
     3961 ATGCTATTCT GAGAAGACAA GAAGACTTTT ATCCATTTTT AAAAGACAAT CGTGAGAAGA
     4021 TTGAAAAAAT CTTGACTTTT CGAATTCCTT ATTATGTTGG TCCATTGGCG CGTGGCAATA
     4081 GTCGTTTTGC ATGGATGACT CGGAAGTCTG AAGAAACAAT TACCCCATGG AATTTTGAAG
     4141 AAGTTGTCGA TAAAGGTGCT TCAGCTCAAT CATTTATTGA ACGCATGACA AACTTTGATA
     4201 AAAATCTTCC AAATGAAAAA GTACTACCAA AACATAGTTT GCTTTATGAG TATTTTACGG
     4261 TTTATAACGA ATTGACAAAG GTCAAATATG TTACTGAGGG AATGCGAAAA CCAGCATTTC
     4321 TTTCAGGTGA ACAGAAGAAA GCCATTGTTG ATTTACTCTT CAAAACAAAT CGAAAAGTAA
     4381 CCGTTAAGCA ATTAAAAGAA GATTATTTCA AAAAAATAGA ATGTTTTGAT AGTGTTGAAA
     4441 TTTCAGGAGT TGAAGATAGA TTTAATGCTT CATTAGGTAC CTACCATGAT TTGCTAAAAA
     4501 TTATTAAAGA TAAAGATTTT TTGGATAATG AAGAAAATGA AGATATCTTA GAGGATATTG
     4561 TTTTAACATT GACCTTATTT GAAGATAGGG AGATGATTGA GGAAAGACTT AAAACATATG
     4621 CTCACCTCTT TGATGATAAG GTGATGAAAC AGCTTAAACG TCGCCGTTAT ACTGGTTGGG
     4681 GACGTTTGTC TCGAAAATTG ATTAATGGTA TTAGGGATAA GCAATCTGGC AAAACAATAT
     4741 TAGATTTTTT GAAATCAGAT GGTTTTGCCA ATCGCAATTT TATGCAGCTG ATCCATGATG
     4801 ATAGTTTGAC ATTTAAAGAA GACATTCAAA AAGCACAAGT GTCTGGACAA GGCGATAGTT
     4861 TACATGAACA TATTGCAAAT TTAGCTGGTA GCCCTGCTAT TAAAAAAGGT ATTTTACAGA
     4921 CTGTAAAAGT TGTTGATGAA TTGGTCAAAG TAATGGGGCG GCATAAGCCA GAAAATATCG
     4981 TTATTGAAAT GGCACGTGAA AATCAGACAA CTCAAAAGGG CCAGAAAAAT TCGCGTGAGC
     5041 GTATGAAACG TATTGAAGAA GGTATCAAAG AATTAGGAAG TCAGATTCTT AAAGAGCATC
     5101 CTGTTGAAAA TACTCAATTG CAAAATGAAA AGCTCTATCT CTATTATCTC CAAAATGGAA
     5161 GAGACATGTA TGTGGACCAA GAATTAGATA TTAATCGTTT AAGTGATTAT GATGTCGATC
     5221 ACATTGTTCC ACAAAGTTTC ATTAAAGACG ATTCAATAGA CAATAAGGTC TTAACGCGTT
     5281 CTGATAAAAA TCGTGGTAAA TCGAATAACG TTCCAAGTGA AGAAGTAGTC AAAAAGATGA
     5341 AAAACTATTG GAGACAACTT CTAAACGCCA AGTTAATCAC TCAACGTAAG TTTGATAATT
     5401 TAACGAAAGC TGAACGTGGA GGTTTGAGTG AACTTGATAA AGCTGGTTTT ATCAAACGCC
     5461 AATTGGTTGA AACTCGCCAA ATCACTAAGC ATGTGGCACA AATTTTGGAT AGTCGCATGA
     5521 ATACTAAATA CGATGAAAAT GATAAACTTA TTCGAGAGGT TAAAGTGATT ACCTTAAAAT
     5581 CTAAATTAGT TTCTGACTTC CGAAAAGATT TCCAATTCTA TAAAGTACGT GAGATTAACA
     5641 ATTACCATCA TGCCCATGAT GCGTATCTTA ATGCCGTCGT TGGAACTGCT TTGATTAAGA
     5701 AATATCCAAA ACTTGAATCG GAGTTTGTCT ATGGTGATTA TAAAGTTTAT GATGTTCGTA
     5761 AAATGATTGC TAAGTCTGAG CAGGAAATAG GCAAAGCAAC CGCAAAATAT TTCTTTTACT
     5821 CTAATATCAT GAACTTCTTC AAAACAGAAA TTACACTTGC AAATGGAGAG ATTCGCAAAC
     5881 GCCCTCTAAT CGAAACTAAT GGGGAAACTG GAGAAATTGT CTGGGATAAA GGGCGAGATT
     5941 TTGCCACAGT GCGCAAAGTA TTGTCCATGC CCCAAGTCAA TATTGTCAAG AAAACAGAAG
     6001 TACAGACAGG CGGATTCTCC AAGGAGTCAA TTTTACCAAA AAGAAATTCG GACAAGCTTA
     6061 TTGCTCGTAA AAAAGACTGG GATCCAAAAA AATATGGTGG TTTTGATAGT CCAACGGTAG
     6121 CTTATTCAGT CCTAGTGGTT GCTAAGGTGG AAAAAGGGAA ATCGAAGAAG TTAAAATCCG
     6181 TTAAAGAGTT ACTAGGGATC ACAATTATGG AAAGAAGTTC CTTTGAAAAA AATCCGATTG
     6241 ACTTTTTAGA AGCTAAAGGA TATAAGGAAG TTAGAAAAGA CTTAATCGTT AAACTACCTA
     6301 AATATAGTCT TTTTGAGTTA GAAAACGGTC GTAAACGGAT GCTGGCTAGT GCCGGAGAAT
     6361 TACAAAAAGG AAATGAGCTG GCTCTGCCAA GCAAATATGT GAATTTTTTA TATTTAGCTA
     6421 GTCATTATGA AAAGTTGAAG GGTAGTCCAG AAGATAACGA ACAAAAACAA TTGTTTGTGG
     6481 AGCAGCATAA GCATTATTTA GATGAGATTA TTGAGCAAAT CAGTGAATTT TCTAAGCGTG
     6541 TTATTTTAGC AGATGCCAAT TTAGATAAAG TTCTTAGTGC ATATAACAAA CATAGAGACA
     6601 AACCAATACG TGAACAAGCA GAAAATATTA TTCATTTATT TACGTTGACG AATCTTGGAG
     6661 CTCCCGCTGC TTTTAAATAT TTTGATACAA CAATTGATCG TAAACGATAT ACGTCTACAA
     6721 AAGAAGTTTT AGATGCCACT TTTATCCATC AATCCATCAC TGGTCTTTAT GAAACACGCA
     6781 TTGATTTGAG TCAGCTAGGA GGTGACTAAG TCGACCTCGA GACTAGTCAA GGTCGGCAAT
     6841 TCTGCAGTAC TAGGACGCCG CCAAGCCAGC TTAAACCCAG CTCAATGAGC TGGGTTTTTT
     6901 GTTTGTTAAA AATGAAGAAG AAACTGTGAA GCGTATTTAG CGTATAAGAG ACAGCCATTC
     6961 TACAGTTTAT TCTTGACATT GCACTGTCCC CCTGGTATAA TAACTATATG AGACCTAGGA
     7021 GGACAAACAT GTCAAAAGGA GAAGAGCTGT TCACAGGTGT TGTGCCGATT CTCGTTGAGC
     7081 TTGACGGAGA TGTAAACGGA CACAAATTCT CTGTTCGCGG TGAAGGTGAA GGAGATGCAA
     7141 CAAACGGCAA GCTGACATTG AAGTTTATTT GCACAACTGG AAAGCTGCCG GTTCCTTGGC
     7201 CGACACTTGT AACGACGCTG ACTTACGGCG TTCAATGCTT CTCTCGTTAT CCAGACCACA
     7261 TGAAACGCCA TGATTTCTTC AAATCTGCAA TGCCTGAAGG CTACGTTCAA GAGCGTACGA
     7321 TCAGCTTCAA AGATGACGGA ACGTACAAAA CAAGAGCAGA AGTGAAGTTT GAAGGTGACA
     7381 CACTTGTGAA CCGCATTGAA TTGAAAGGCA TTGATTTCAA AGAAGATGGA AACATCCTTG
     7441 GACACAAACT TGAATACAAC TTCAACAGCC ACAACGTATA CATCACTGCT GACAAACAAA
     7501 AAAACGGCAT CAAAGCAAAC TTCAAAATCC GTCATAACGT AGAGGACGGT TCTGTTCAGC
     7561 TTGCTGATCA TTATCAGCAA AATACACCGA TCGGTGACGG CCCGGTTCTT CTTCCTGATA
     7621 ACCATTATTT ATCAACTCAA AGCGTATTAT CAAAAGACCC AAATGAAAAG CGTGACCACA
     7681 TGGTGCTGCT TGAATTTGTG ACAGCTGCTG GTATCACTCA CGGCATGGAT GAGCTTTATA
     7741 AGTAAGGTCT CGGTTTAAGA GCTATGCTGG AAACAGCATA GCAAGTTTAA ATAAGGCTAG
     7801 TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTTCTGTC TCAATCGCAA
     7861 CATCAAACCA AAATAAAAAC CCCCTTCGAC TTTCGTCAGG GGGCTTTTAT TTATTCAATA
     7921 ATCCCTCCTC TCAATAAATC TATTGTTGTA CTTAATTCAA CTTCCATTTC TCTGTATCTT
     7981 TCAATACGCT CTTTTAAGTC CTTAATTTCT TTTTTTAATT CCTCATTTTC AGCAAATAAC
     8041 TCTTTTTCTT TGTTTGTCAT TTTATTTCCC CCGTTTCAGC ATCAAGAACC TTTGCATAAC
     8101 TTGCTCTATA TCCACACTGA TAATTGCCCT CAAACCATAA TCTAAAGGCG CTAGAGTTTG
     8161 TTGAAACAAT ATCTTTTACA TCATTCGTAT TTAAAATTCC AAACTCCGCT CCCCTAAGGC
     8221 GAATAAAAGC CATTAAATCT TTTGTATTTA CCAAATTATA GTCATCCACT ATATCTAAAA
     8281 GTAAATTCTT CAATTCTCTT TTTTGGCTTT CATCAAGTGT TATATAGCGG TCAATATCAA
     8341 AATCATTAAT GTTCAAAATA TCTTTTTTGT CGTATATATG TTTATTCTTA GCAATAGCGT
     8401 CCTTTGATTC ATGAGTCAAA TATTCATATG AACCTTTGAT ATAATCAAGT ATCTCAACAT
     8461 GAGCAACTGA ACTATTCCCC AATTTTCGCT TAATCTTGTT CCTAACGCTT TCTATTGTTA
     8521 CAGGATTTCG TGCAATATAT ATAACGTGAT AGTGTGGTTT TTTATAGTGC TTTCCATTTT
     8581 GTATAATATT ACTATTATTC CATGTATCTT TATCTTTTTT TTCGTCCATA TCGTGTAAAG
     8641 GACTGACAGC CATAGATACG CCCAAACTCT CTAATTTTTC TTTCCAATCA TTAGGAATTG
     8701 AGTCAGGATA TAATAAAAAT CCAAAATTTC TAGCTTTAGT ATTTTTAATA GCCATGATAT
     8761 AATTACCTTA TCAAAAACAA GTAGCGAAAA CTCGTATCCT TCTAAAAACG CGAGCTTTCG
     8821 CTTATTTTTT TTGTTCTGAT TCCTTTCTTG CATATTCTTC TATAGCTAAC GCCGCAACCG
     8881 CAGATTTTGA AAAACCTTTT TGTTTCGCCA TATCTGTTAA TTTTTTATCT TGCTCTTTTG
     8941 TCAGAGAAAT CATAACTCTT TTTTTCGATT CTGAAATCAC CATTTAAAAA ACTCCAATCA
     9001 AATAATTTTA TAAAGTTAGT GTATCACTTT GTAATCATAA AAACAACAAT AAAGCTACTT
     9061 AAATATAGAT TTATAAAAAA CGTTGGCGAA AACGTTGGCG ATTCGTTGGC GATTGAAAAA
     9121 CCCCTTAAAC CCTTGAGCCA GTTGGGATAG AGCGTTTTTG GCACAAAAAT TGGCACTCGG
     9181 CACTTAATGG GGGGTCGTAG TACGGAAGCA AAATTCGCTT CCTTTCCCCC CATTTTTTTC
     9241 CAAATTCCAA ATTTTTTTCA AAAATTTTCC AGCGCTACCG CTCGGCAAAA TTGCAAGCAA
     9301 TTTTTAAAAT CAAACCCATG AGGGAATTTC ATTCCCTCAT ACTCCCTTGA GCCTCCTCCA
     9361 ACCGAAATAG AAGGGCGCTG CGCTTATTAT TTCATTCAGT CATCGGCTTT CATAATCTAA
     9421 CAGACAACAT CTTCGCTGCA AAGCCACGCT ACGCTCAAGG GCTTTTACGC TACGATAACG
     9481 CCTGTTTTAA CGATTATGCC GATAACTAAA CGAAATAAAC GCTAAAACGT CTCAGAAACG
     9541 ATTTTGAGAC GTTTTAATAA AAAATCGGAT AATAAATATA TATAAACGTA TATAGATTTC
     9601 ATAAAGTCTA ACACACTAGA CTTATTTACT TCGTAATTAA GTCGTTAAAC CGTGTGCTCT
     9661 ACGACCAAAA CTATAAAACC TTTAAGAACT TTCTTTTTTT ACAAGAAAAA AGAAATTAGA
     9721 TAAATCTCTC ATATCTTTTA TTCAATAATC GCATCCGATT GCAGTATAAA TTTAACGATC
     9781 ACTCATCATG TTCATATTTA TCAGGATCCT CTAGAGTCGC GCAAAAGAAA AACGAAATGA
     9841 TACACCAATC AGTGCAAAAA AAGATATAAT GGGAGATAAG ACGGTTCGTG TTCGTGCTGA
     9901 CTTGCACCAT ATCATAAAAA TCGAAACAGC AAAGAATGGC GGAAACGTAA AAGAAGTTAT
     9961 GGAAATAAGA CTTAGAAGCA AACTTAAGAG TGTGTTGATA GTGCAGTATC TTAAAATTTT
    10021 GTATAATAGG AATTGAAGTT AAATTAGATG CTAAAAATTT GTAATTAAGA AGGAGTGATT
    10081 AC
//