LOCUS       Exported               10453 bp ds-DNA     circular SYN 15-MAY-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   laosan126
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 6, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10453
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     94..127
                     /label=pw-CBE-A3AN57G-F(NcoI)
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     344..403
                     /label=ACBE-N-AID 5.0-F(NotI)
     misc_feature    375..380
                     /label=Kozak
     misc_feature    384..437
                     /label=bp-NLS
     misc_feature    438..1031
                     /label=hA3A
     primer_bind     complement(1009..1058)
                     /label=pw-A3A n57g -Rad51 2.0-R1(in)
     CDS             1032..1127
                     /codon_start=1
                     /label=Linker1
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     primer_bind     1032..1055
                     /label=pw-A3A n57g -Rad51 2.0-F2(in)
     CDS             1128..1469
                     /codon_start=1
                     /label=Human-RAD51-1-114
                     /translation="MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAG
                     FHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITT
                     GSKELDKLLQ"
     primer_bind     complement(1442..1494)
                     /label=AID-RAD51-Cas9n-R(in)-2
     CDS             1470..1565
                     /codon_start=1
                     /label=Linker2
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     primer_bind     1470..1494
                     /label=AID-RAD51-Cas9n-F(in)-3
     primer_bind     1541..1590
                     /label=pw-A3A n57g -Rad51 2.0-F3(in)
     primer_bind     complement(1543..1565)
                     /label=pw-A3A n57g -Rad51 2.0-R2(in)
     misc_feature    1566..5666
                     /label=Cas9n
     primer_bind     1750..1769
                     /label=ACBE-N-AID 5.0-F-seq-F3
     primer_bind     complement(1818..1845)
                     /label=Primer 1
     primer_bind     2270..2290
                     /label=ACBE-N-AID 5.0-F-seq-F4
     primer_bind     2820..2838
                     /label=ACBE-N-AID 5.0-F-seq-F5
     primer_bind     3414..3435
                     /label=ACBE-N-AID 5.0-F-seq-F6
     primer_bind     4013..4033
                     /label=ACBE-N-AID 5.0-F-seq-F7
     primer_bind     4594..4614
                     /label=ACBE-N-AID 5.0-F-seq-F8
     primer_bind     5521..5542
                     /label=ACBE-N-AID 5.0-F-seq-F10
     primer_bind     complement(5642..5680)
                     /label=ACBE-N-AID 6.1-R(in)
     primer_bind     5653..5689
                     /label=ACBE-N-AID 6.1-F(in)
     misc_feature    5667..5696
                     /label=linker
     misc_feature    5697..5945
                     /label=ugi
     misc_feature    5976..6224
                     /label=ugi
     misc_feature    6237..6287
                     /label=bp-NLS
     CDS             6267..6287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6297..6353
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6354..7070
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7076..7134)
                     /label=ACBE-N-AID 6.2-R(PmeI)
     CDS             7083..7103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7132..7356
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7350..7369)
                     /label=T7-ABE7.10-R
     primer_bind     complement(7427..7443)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7451..7467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7475..7505)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7520..7541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7829..8417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8588..9448)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9449..9553)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9843..10083
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        10110..10185
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        join(10205..10453,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgccacc atgaagagga ccgccgatgg ctctgagttc gagagcccca
      421 agaagaagcg gaaggtggag gcatctccag caagcggacc aaggcacctg atggaccccc
      481 acatcttcac ctctaacttt aacaatggca tcggcaggca caagacatac ctgtgctatg
      541 aggtggagcg cctggacaac ggcaccagcg tgaagatgga tcagcacaga ggcttcctgc
      601 acaaccaggc caagaatctg ctgtgcggct tctacggccg gcacgcagag ctgagatttc
      661 tggacctggt gcctagcctg cagctggatc cagcccagat ctatagggtg acctggttca
      721 tcagctggtc cccatgcttt tcctggggat gtgcaggaga ggtgcgcgcc ttcctgcagg
      781 agaatacaca cgtgcggctg agaatctttg ccgcccggat ctacgactat gatcctctgt
      841 acaaggaggc cctgcagatg ctgagagacg caggagccca ggtgtccatc atgacctatg
      901 atgagttcaa gcactgctgg gacacatttg tggatcacca gggctgtccc tttcagcctt
      961 gggacggact ggatgagcac tcccaggccc tgtctggcag gctgagggcc atcctgcaga
     1021 accagggcaa tagcggagga tctagcggag gatcaagcgg aagcgagact cctggaacca
     1081 gcgaaagcgc aaccccagaa agcagcggag gaagtagcgg aggaagcatg gcaatgcaga
     1141 tgcagcttga agcaaatgca gatacttcag tggaagaaga aagctttggc ccacaaccca
     1201 tttcacggtt agagcagtgt ggcataaatg ccaacgatgt gaagaaattg gaagaagctg
     1261 gattccatac tgtggaggct gttgcctatg cgccaaagaa ggagctaata aatattaagg
     1321 gaattagtga agccaaagct gataaaattc tggctgaggc agctaaatta gttccaatgg
     1381 gtttcaccac tgcaactgaa ttccaccaaa ggcggtcaga gatcatacag attactactg
     1441 gctccaaaga gcttgacaaa ctacttcaat ctggcggatc tagcggtgga tctagcggct
     1501 ctgagacccc tggaacatcc gaatccgcca ctccagagag cagcggaggc tcttctggag
     1561 gatcagacaa gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg
     1621 tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc
     1681 ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg
     1741 aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct
     1801 gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca
     1861 gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg
     1921 gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa
     1981 agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc
     2041 acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg
     2101 acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc
     2161 ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca
     2221 gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa
     2281 acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg
     2341 aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg
     2401 cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca
     2461 tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct
     2521 ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc
     2581 ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg
     2641 ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc
     2701 tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc
     2761 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc
     2821 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga
     2881 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca
     2941 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg
     3001 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata
     3061 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg
     3121 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc
     3181 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga
     3241 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa
     3301 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa
     3361 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg
     3421 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg
     3481 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg
     3541 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc
     3601 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg
     3661 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc
     3721 tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga
     3781 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg
     3841 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga
     3901 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc
     3961 ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc
     4021 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc
     4081 atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa
     4141 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga
     4201 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc
     4261 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac
     4321 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga
     4381 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt
     4441 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca
     4501 actaccacca cgcccacgac gcctacctaa acgccgtcgt gggaaccgcc ctgatcaaaa
     4561 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga
     4621 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca
     4681 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc
     4741 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt
     4801 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg
     4861 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga
     4921 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg
     4981 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg
     5041 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg
     5101 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta
     5161 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac
     5221 tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca
     5281 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg
     5341 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag
     5401 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata
     5461 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag
     5521 cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca
     5581 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga
     5641 tcgacctgtc tcagctggga ggtgacagcg gcgggagcgg cgggagcggg gggagcacta
     5701 atctgagcga catcattgag aaggagactg ggaaacagct ggtcattcag gagtccatcc
     5761 tgatgctgcc tgaggaggtg gaggaagtga tcggcaacaa gccagagtct gacatcctgg
     5821 tgcacaccgc ctacgacgag tccacagatg agaatgtgat gctgctgacc tctgacgccc
     5881 ccgagtataa gccttgggcc ctggtcatcc aggattctaa cggcgagaat aagatcaaga
     5941 tgctgagcgg aggatccgga ggatctggag gcagcaccaa cctgtctgac atcatcgaga
     6001 aggagacagg caagcagctg gtcatccagg agagcatcct gatgctgccc gaagaagtcg
     6061 aagaagtgat cggaaacaag cctgagagcg atatcctggt ccataccgcc tacgacgaga
     6121 gtaccgacga aaatgtgatg ctgctgacat ccgacgcccc agagtataag ccctgggctc
     6181 tggtcatcca ggattccaac ggagagaaca aaatcaaaat gctgtctggc ggctcaaaaa
     6241 gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtcgga agcggagcta
     6301 ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga cctatggtga
     6361 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     6421 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     6481 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     6541 ccaccctgac ctatggagtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     6601 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     6661 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     6721 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     6781 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     6841 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     6901 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     6961 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     7021 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tctggtggtt
     7081 ctcccaagaa gaaaaggaaa gtctaagttt aaacccgctg atcagcctcg actgtgcctt
     7141 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     7201 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     7261 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga
     7321 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     7381 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7441 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7501 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7561 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7621 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7681 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7741 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7801 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7861 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7921 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7981 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8041 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8101 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8161 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8221 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8281 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8341 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8401 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8461 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8521 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8581 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8641 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8701 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8761 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8821 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8881 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8941 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9001 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9061 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9121 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9181 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9241 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9301 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9361 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9421 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9481 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9541 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     9601 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     9661 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     9721 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     9781 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tggatccctg cagttaatta
     9841 aagagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
     9901 agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
     9961 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
    10021 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
    10081 gacgaaacac cgggtcttcg agaagacctg ttttagagct agaaatagca agttaaaata
    10141 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttatgcatgg
    10201 taccgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
    10261 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
    10321 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
    10381 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
    10441 catcaagtgt atc
//
