LOCUS       Exported               11101 bp ds-DNA     circular SYN 06-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   laosan120
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 6, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     344..403
                     /label=ACBE-N-AID 5.0-F(NotI)
     misc_feature    375..380
                     /label=Kozak
     misc_feature    381..980
                     /label=bpNLS-AID-genescript-human
                     /label=genescript-human
     misc_feature    384..437
                     /label=bp-NLS
     misc_feature    438..980
                     /label=AID
     primer_bind     complement(956..1014)
                     /label=ACBE-N-AID 5.7-R(in)
     primer_bind     989..1047
                     /label=ACBE-N-AID 5.7-F(in)
     CDS             1026..1523
                     /codon_start=1
                     /label=Tad-WT
                     /translation="SEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGWN
                     RPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVFG
                     ARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQS
                     STD"
     primer_bind     1026..1052
                     /label=ACBE-N-AID 5.0-F(in)-2
     primer_bind     complement(1096..1123)
                     /label=ACBE-N-AID-R(BsmbI)
     primer_bind     1191..1211
                     /label=ACBE-N-AID 5.0-F-seq-F1
     primer_bind     1217..1236
                     /label=ACBE-N-AID 5.0-F-seq-F2
     CDS             1524..1619
                     /codon_start=1
                     /label=Linker1
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             1620..2117
                     /codon_start=1
                     /label=Tad-Mutant
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     VRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECAALLCYFFRMPRQVFNAQKKAQS
                     STD"
     primer_bind     complement(1690..1717)
                     /label=ACBE-N-AID-R(BsmbI)
     primer_bind     1811..1830
                     /label=ACBE-N-AID 5.0-F-seq-F2
     CDS             2118..2213
                     /codon_start=1
                     /label=Linker2
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     primer_bind     complement(2189..2226)
                     /label=ACBE-N-AID 5.0-R(in)-2
     primer_bind     2197..2239
                     /label=ACBE-N-AID 5.0-F(in)-3
     CDS             2214..4450
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A) from px330
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDE"
     primer_bind     2398..2417
                     /label=ACBE-N-AID 5.0-F-seq-F3
     primer_bind     2918..2938
                     /label=ACBE-N-AID 5.0-F-seq-F4
     primer_bind     3468..3486
                     /label=ACBE-N-AID 5.0-F-seq-F5
     primer_bind     4062..4083
                     /label=ACBE-N-AID 5.0-F-seq-F6
     primer_bind     complement(4354..4364)
                     /label=ACBE-N-AID 5.0-F(in)-3
     primer_bind     4420..4445
                     /label=ACBE-N-AID 6.2-F(SacI)
     CDS             4451..6314
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9n
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10 mutation in the RuvC-like catalytic domain"
                     /translation="VKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELG
                     SQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDS
                     IDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSE
                     LDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKD
                     FQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQE
                     IGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVL
                     SMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFE
                     LENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKH
                     YLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAA
                     FKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     4661..4681
                     /label=ACBE-N-AID 5.0-F-seq-F7
     primer_bind     5242..5262
                     /label=ACBE-N-AID 5.0-F-seq-F8
     primer_bind     6169..6190
                     /label=ACBE-N-AID 5.0-F-seq-F10
     primer_bind     complement(6290..6328)
                     /label=ACBE-N-AID 6.1-R(in)
     primer_bind     6301..6337
                     /label=ACBE-N-AID 6.1-F(in)
     misc_feature    6315..6344
                     /label=linker
     misc_feature    6345..6593
                     /label=ugi
     misc_feature    6624..6872
                     /label=ugi
     misc_feature    6885..6935
                     /label=bp-NLS
     CDS             6915..6935
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6945..7001
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7002..7718
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7724..7782)
                     /label=ACBE-N-AID 6.2-R(PmeI)
     CDS             7731..7751
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7780..8004
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7998..8017)
                     /label=T7-ABE7.10-R
     primer_bind     complement(8075..8091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8099..8115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8123..8153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8168..8189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8477..9065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9236..10096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10097..10201)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10491..10731
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        10758..10833
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        join(10853..11101,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgccacc atgaagagga ccgccgatgg ctctgagttc gagagcccca
      421 agaagaagcg gaaggtggac agcctgctga tgaaccggag aaagttcctg tatcagttta
      481 agaatgtgag gtgggcaaag ggcaggcgcg agacctacct gtgctatgtg gtgaagcgga
      541 gagatagcgc cacatctttc agcctggact ttggctacct gcggaacaag aatggctgcc
      601 acgtggagct gctgtttctg agatacatct ccgactggga tctggaccca ggcaggtgtt
      661 atcgcgtgac ctggttcaca tcctggtctc cctgctacga ttgtgcccgg cacgtggccg
      721 actttctgag aggcaaccct aatctgtctc tgaggatctt caccgcccgc ctgtattttt
      781 gcgaggatag gaaggccgag ccagagggac tgaggcgcct gcacagggcc ggcgtgcaga
      841 tcgccatcat gacattcaag gactactttt attgttggaa caccttcgtg gagaatcacg
      901 agcggacatt taaggcctgg gagggactgc acgagaactc cgtgcggctg tctagacagc
      961 tgcggagaat cctgctgcca gaggccgccg ccaaggaagc tgccgccaag gaggccgccg
     1021 ccaagagcga ggtggagttc agccacgagt actggatgag acacgccctg accctggcta
     1081 agagagcttg ggatgagaga gaggtgcccg tgggagctgt tctggttcat aacaacaggg
     1141 tgatcggcga gggatggaac agacctatcg ggagacacga cccaaccgct catgctgaaa
     1201 tcatggccct gagacaagga gggctggtga tgcaaaatta cagactgatc gacgcaaccc
     1261 tgtacgtgac cctggagcct tgtgtgatgt gcgcaggagc aatgatccac tccagaatcg
     1321 gcagagtggt gttcggagct agagatgcca aaaccggagc cgctggaagc ctgatggacg
     1381 ttctgcatca ccccggaatg aatcacagag tggagataac cgagggcatt ctggccgacg
     1441 agtgtgctgc tctgctgtct gatttcttca gaatgagaag gcaggaaatc aaggcccaga
     1501 aaaaggccca aagcagcacc gacagcggag gatctagcgg aggatcaagc ggaagcgaga
     1561 ctcctggaac cagcgaaagc gcaaccccag aaagcagcgg aggaagtagc ggaggaagct
     1621 cagaagtcga gttcagccat gagtattgga tgagacatgc tctgaccctg gcaaagagag
     1681 caagagacga gagagaggtc ccagtgggag cagttctggt gctgaacaac agagtgatcg
     1741 gggaggggtg gaacagagca atcggactgc acgaccctac agcacacgca gaaataatgg
     1801 cactgagaca aggggggctc gtgatgcaaa actacaggct gatcgacgcc accctgtacg
     1861 tcacatttga gccctgtgtg atgtgtgccg gagccatgat tcacagtaga atcggccggg
     1921 tggtgttcgg tgtgagaaac gctaaaacag gcgccgccgg aagcctgatg gatgttctgc
     1981 attaccccgg catgaatcac cgggtggaga tcacagaggg catcctggct gacgaatgtg
     2041 ccgctctgct gtgttacttc ttcagaatgc cccgacaagt gttcaacgcc cagaagaaag
     2101 cccagtcaag caccgactct ggcggatcta gcggtggatc tagcggctct gagacccctg
     2161 gaacatccga atccgccact ccagagagca gcggaggctc ttctggagga tcagacaaga
     2221 agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg
     2281 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
     2341 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc
     2401 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag
     2461 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt
     2521 ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg
     2581 acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg
     2641 acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt
     2701 tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc
     2761 tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca
     2821 gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa
     2881 atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc
     2941 tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac
     3001 tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg
     3061 accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg
     3121 acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga
     3181 gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc
     3241 ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg
     3301 acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg
     3361 acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga
     3421 ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc
     3481 ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc
     3541 tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct
     3601 ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca
     3661 agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca
     3721 acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc
     3781 tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc
     3841 agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc
     3901 tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg
     3961 aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca
     4021 aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga
     4081 cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg
     4141 acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc
     4201 ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga
     4261 agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct
     4321 ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca
     4381 ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg
     4441 tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg
     4501 ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga
     4561 tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca
     4621 cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg
     4681 tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc
     4741 agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc
     4801 ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc
     4861 ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg
     4921 agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa
     4981 cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg
     5041 acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt
     5101 ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg
     5161 cccacgacgc ctacctaaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc
     5221 tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca
     5281 agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga
     5341 actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg
     5401 agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc
     5461 ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg
     5521 gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga
     5581 aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc
     5641 tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc
     5701 tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag
     5761 ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt
     5821 tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa
     5881 acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga
     5941 agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc
     6001 actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg
     6061 acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag
     6121 agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct
     6181 tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg
     6241 acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc
     6301 agctgggagg tgacagcggc gggagcggcg ggagcggggg gagcactaat ctgagcgaca
     6361 tcattgagaa ggagactggg aaacagctgg tcattcagga gtccatcctg atgctgcctg
     6421 aggaggtgga ggaagtgatc ggcaacaagc cagagtctga catcctggtg cacaccgcct
     6481 acgacgagtc cacagatgag aatgtgatgc tgctgacctc tgacgccccc gagtataagc
     6541 cttgggccct ggtcatccag gattctaacg gcgagaataa gatcaagatg ctgagcggag
     6601 gatccggagg atctggaggc agcaccaacc tgtctgacat catcgagaag gagacaggca
     6661 agcagctggt catccaggag agcatcctga tgctgcccga agaagtcgaa gaagtgatcg
     6721 gaaacaagcc tgagagcgat atcctggtcc ataccgccta cgacgagagt accgacgaaa
     6781 atgtgatgct gctgacatcc gacgccccag agtataagcc ctgggctctg gtcatccagg
     6841 attccaacgg agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg
     6901 gcagcgaatt cgagcccaag aagaagagga aagtcggaag cggagctact aacttcagcc
     6961 tgctgaagca ggctggagac gtggaggaga accctggacc tatggtgagc aagggcgagg
     7021 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     7081 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     7141 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     7201 atggagtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     7261 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     7321 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     7381 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     7441 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     7501 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     7561 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     7621 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     7681 ccgccgggat cactctcggc atggacgagc tgtacaagtc tggtggttct cccaagaaga
     7741 aaaggaaagt ctaagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     7801 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7861 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     7921 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg
     7981 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatac
     8041 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8101 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctagg
     8161 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8281 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8341 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8401 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8461 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8521 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8581 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8641 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8701 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8761 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8821 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8881 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8941 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9001 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9061 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9121 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9181 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9241 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9301 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9361 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9421 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9481 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9541 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9601 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9661 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9721 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9781 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9841 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9901 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9961 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10021 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10081 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10141 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10201 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc
    10261 ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
    10321 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
    10381 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
    10441 ttcgcgatgt acgggccaga tatacgcgtg gatccctgca gttaattaaa gagggcctat
    10501 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa
    10561 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
    10621 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
    10681 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
    10741 ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
    10801 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt atgcatggta ccgacattga
    10861 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
    10921 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
    10981 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
    11041 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
    11101 c
//
