LOCUS       AWp28-v1_scaffol        4374 bp    DNA     circular UNA 02-SEP-2019
DEFINITION  pBT264-U6-scaffold v1 storage vector.
ACCESSION   urn.local...pw-89rvf6o
VERSION     urn.local...pw-89rvf6o
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..2981
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1..2711
                     /note="Geneious type: concatenated sequence"
     primer_bind     complement(9..31)
     primer_bind     complement(11..30)
     primer_bind     11..30
     misc_feature    26..125
     CDS             join(27..57,58..986)
                     /gene="AMP"
     misc_feature    27..986
     CDS             56..84
                     /gene="Ampp"
     promoter        56..84
     promoter        56..79
     misc_feature    56..84
     promoter        57..84
     CDS             complement(83..1083)
                     /gene="AMP"
     gene            126..986
     CDS             126..986
                     /gene="Amp"
     misc_feature    126..922
     misc_feature    127..986
     primer_bind     complement(130..153)
     misc_feature    complement(172..959)
                     /note="Geneious type: ORF"
     misc_feature    <225..234
                     /note="Geneious type: concatenated sequence"
     primer_bind     complement(234..254)
     primer_bind     complement(661..684)
     misc_feature    945..967
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(1222..1245)
     primer_bind     complement(1646..1665)
     primer_bind     complement(1743..1762)
     misc_feature    1802..1824
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1932..1953
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1934..1953
                     /note="Geneious type: primer_bind_reverse"
     promoter        2069..2098
     misc_feature    2070..2098
     misc_feature    2112..2134
     primer_bind     complement(2115..2148)
     primer_bind     2131..2147
     misc_feature    2133..2151
     misc_feature    2134..2151
     misc_feature    2140..2162
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2205..2454
     misc_feature    2213..2454
     misc_feature    complement(2372..2391)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2428..2447)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2455..2704
     misc_feature    2463..2704
     misc_feature    complement(2622..2641)
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2657..2678
     misc_feature    complement(2678..2697)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    <2702..2711
                     /note="Geneious type: concatenated sequence"
     misc_feature    <2708..2712
                     /note="Geneious type: Editing History Insertion"
     misc_feature    2708..2711
                     /note="Geneious type: ligation"
     misc_feature    2712..2977
                     /note="Geneious type: Concatenated sequence"
     promoter        2713..2963
                     /note="Geneious type: Promoter"
     promoter        2714..2961
                     /note="Geneious type: Promoter"
     primer_bind     2823..2851
     primer_bind     2940..2963
     misc_feature    2963..>2977
                     /note="Geneious type: Editing History Insertion"
     misc_feature    2978..2981
                     /note="Geneious type: ligation"
     misc_feature    2978..3095
                     /note="Geneious type: Concatenated sequence"
     primer_bind     2978..2997
     misc_feature    <2997..3091
                     /note="Geneious type: Concatenated sequence"
     primer_bind     2997..3031
     misc_feature    2997..3073
     misc_feature    complement(3079..3098)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    <3092..>3105
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3092..3095
                     /note="Geneious type: ligation"
     misc_feature    3092..4374
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <3092..4374
                     /note="Geneious type: concatenated sequence"
     misc_feature    <3092..3105
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <3092..3105
                     /note="Geneious type: concatenated sequence"
     misc_feature    <3092..>3095
                     /note="Geneious type: concatenated sequence"
     misc_feature    3097..3104
     misc_difference <3106..3481
     misc_feature    3106..3109
                     /note="Geneious type: ligation"
     misc_feature    3110..2707
                     /note="Geneious type: Concatenated sequence"
     polyA_signal    3150..3200
     misc_feature    complement(3156..3175)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3199..3218)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    3215..3456
     misc_feature    complement(3374..3393)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3430..3449)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    3457..3706
     misc_feature    3465..3706
     misc_feature    complement(3624..3643)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3680..3699)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(3756..3773)
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      3931..4237
     misc_feature    3931..4237
     primer_bind     complement(3990..4009)
     primer_bind     complement(4018..4038)
     primer_bind     complement(4131..4152)
     primer_bind     complement(4144..4163)
ORIGIN      
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctgga gctgatttaa atggggacag
     2221 ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg
     2281 agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg
     2341 cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg
     2401 ctgctctttg agcctgcaga cacctggggg gatacgggga aaaagcttta ggctgagtgc
     2461 acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg
     2521 ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc
     2581 cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga
     2641 acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga aaaagcttta
     2701 ggctgaagat ctagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     2761 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
     2821 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     2881 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     2941 cttgtggaaa ggacgaaaca ccgggtcttc gctgcactcg agtgcagcga agacctgttt
     3001 tagaggatga aaatccaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
     3061 ccgagtcggt gcttttttgg atccgcaacg gaattcctga cgcacaattg ttgttgttaa
     3121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3181 taaagcattt ttttcactgc ctcgaatcac tagtggggac agcccccccc caaagccccc
     3241 agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc
     3301 tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca gcccgggcac
     3361 ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt tgagcctgca
     3421 gacacctggg gggatacggg gaaaaagctt taggctgagt gcacggggac agcccccccc
     3481 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     3541 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     3601 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     3661 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgagg cgcgcctgta
     3721 cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
     3781 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3841 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3901 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3961 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4021 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4081 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4141 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4201 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4261 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4321 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtg
//
