LOCUS       AWp40_(Expected)        8899 bp    DNA     circular UNA 13-FEB-2020
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...pm-89rvf6m
VERSION     urn.local...pm-89rvf6m
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    0^1
                     /note="Geneious type: Editing History Deletion"
     misc_feature    4..20
                     /note="Geneious type: primer"
     promoter        4..254
                     /note="Geneious type: Promoter"
     misc_feature    5..257
                     /note="Geneious type: Concatenated sequence"
     misc_feature    complement(79..104)
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     184..205
     misc_feature    complement(235..254)
                     /note="Geneious type: primer"
     misc_feature    236..254
                     /note="Geneious type: primer"
     misc_feature    270..962
                     /note="Geneious type: ORF"
     misc_feature    305..324
     misc_feature    335..354
     misc_feature    341..360
     misc_signal     974..8899
                     /gene="FuGW backbone"
     CDS             995..1582
                     /gene="WPRE"
                     /product="The WPRE seems to increase the expression of the
                     marker gene when introduced in its 3' untranslated region.
                     This element is believed to exert some posttranslational
                     regulation."
     misc_feature    995..1582
     misc_feature    complement(1046..1066)
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1192..1226
     misc_feature    complement(1207..1226)
                     /note="Geneious type: primer_bind_reverse"
     CDS             1769..1790
                     /gene="U3PPT"
                     /product="reverse transcriptase-associated RNase H
                     cleavage of the RNA at the polypurine tract (PPT), a short
                     (ca. 11 to 19 nucleotides [nt]) string of purines located
                     at the upstream border of the 3��long terminal repeat"
     CDS             1769..1784
                     /gene="cPPT"
     misc_feature    1769..1790
     misc_feature    1769..1784
     LTR             2106..2286
     CDS             2106..2286
                     /gene="5'LTR; del3'LTR"
                     /product="HIV-1_5_LTR; truncHIV-1_3_LTR"
     misc_feature    2303..2513
                     /gene="bGH pA"
     terminator      2315..2542
     terminator      2315..2542
                     /note="Geneious type: Terminator"
     CDS             2315..2542
                     /gene="bGH-Ter"
                     /product="bGH_PA_terminator"
     polyA_signal    2318..2542
     rep_origin      2605..2911
     misc_feature    2605..2911
     primer_bind     complement(2664..2683)
     primer_bind     complement(2692..2712)
     primer_bind     complement(2805..2826)
     primer_bind     complement(2818..2837)
     misc_signal     complement(3031..3246)
                     /gene="SV40_enhancer"
     misc_feature    complement(3031..3246)
                     /gene="SV40_enhancer"
     CDS             3043..3311
                     /gene="SV40p"
     misc_feature    3043..3311
     rep_origin      3210..3287
     misc_feature    3210..3287
                     /gene="SV40-Ori"
     promoter        3405..3472
     CDS             3405..3472
                     /gene="EM7p"
     promoter        3407..3472
     gene            3473..3844
     misc_feature    3473..3847
                     /note="Geneious type: ORF"
     CDS             3473..3844
                     /gene="Bleo"
     misc_feature    3474..3847
     misc_feature    3474..3844
     terminator      3980..4099
     CDS             3980..4099
                     /gene="SV40-pA"
     misc_feature    3980..4099
     polyA_signal    4008..4058
     misc_feature    complement(4014..4033)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4132..4154)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4143..4161)
     misc_feature    complement(4143..4160)
     primer_bind     4146..4179
     primer_bind     complement(4147..4163)
     misc_feature    complement(4160..4182)
     promoter        complement(4196..4225)
     promoter        complement(4196..4225)
                     /note="Geneious type: Promoter"
     misc_feature    complement(4196..4224)
     misc_feature    complement(4341..4362)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4341..4360)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4470..4492)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4490..5163)
                     /note="Geneious type: ORF"
     primer_bind     4532..4551
     gene            4534..5153
     rep_origin      complement(4534..5153)
     CDS             4534..5153
                     /gene="pBR322  Ori"
     primer_bind     4629..4648
     primer_bind     5049..5072
     CDS             5211..6211
                     /gene="AMP"
     gene            complement(5308..6168)
     CDS             join(complement(6237..6267),complement(5308..6236))
                     /gene="AMP"
     CDS             complement(5308..6168)
                     /gene="Amp"
     misc_feature    complement(5308..6267)
     misc_feature    complement(5308..6167)
     misc_feature    complement(5327..5349)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5335..6122
                     /note="Geneious type: ORF"
     misc_feature    complement(5372..6168)
     primer_bind     5610..5633
     primer_bind     6040..6060
     primer_bind     6141..6164
     misc_feature    complement(6169..6268)
     promoter        complement(6210..6238)
     promoter        complement(6210..6237)
     CDS             complement(6210..6238)
                     /gene="Ampp"
     misc_feature    complement(6210..6238)
     promoter        complement(6215..6238)
     primer_bind     6263..6285
     primer_bind     6264..6283
     primer_bind     complement(6264..6283)
     promoter        6540..7116
     CDS             6540..7116
                     /gene="CMVp"
                     /product="CMV_immearly_promoter"
     primer_bind     6555..6592
     misc_signal     6619..6906
                     /gene="CAG_enhancer"
     misc_feature    6619..6906
                     /gene="CAG_enhancer"
     misc_feature    complement(6631..6652)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6631..6906)
     primer_bind     7073..7093
     LTR             7136..7325
     LTR             7136..7316
     CDS             7136..7316
                     /gene="5'LTR; del3'LTR"
                     /product="HIV-1_5_LTR; truncHIV-1_3_LTR"
     primer_bind     7368..7392
     CDS             7427..7471
                     /gene="Psi"
     misc_feature    7427..7472
     misc_feature    7427..7471
     primer_bind     complement(7576..7600)
     misc_feature    7859..8746
                     /note="Geneious type: ORF"
     CDS             7981..8214
                     /gene="RRE"
     misc_feature    7981..8214
     misc_feature    8741..8858
     CDS             8745..8760
                     /gene="cPPT"
     misc_feature    8745..8760
     primer_bind     8766..8795
     primer_bind     8860..8889
     misc_feature    8873..8894
                     /note="Geneious type: primer"
     misc_feature    8880..8899
                     /note="Geneious type: primer bind"
     misc_feature    8880..20
                     /note="Geneious type: primer"
     misc_feature    8880..8899
                     /note="Geneious type: primer"
ORIGIN      
        1 taatcgagtg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
       61 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
      121 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      181 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      241 gtgtcgtgac gcgggatcta gaggccacca tgagcgagct gattaaggag aacatgcaca
      301 tgaaactcta tatggaagga actgtggaca accaccattt taaatgtacc agtgagggag
      361 agggcaagcc ctacgagggt acacaaacta tgcgcattaa agtggtcgag ggcggccctc
      421 tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca
      481 accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg
      541 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
      601 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc
      661 ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg
      721 acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga
      781 tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg
      841 gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg
      901 tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcact
      961 aatagcctgc aggcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     1021 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     1081 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     1141 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     1201 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     1261 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     1321 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     1381 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     1441 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     1501 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     1561 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag
     1621 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     1681 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     1741 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     1801 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     1861 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     1921 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
     1981 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
     2041 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
     2101 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     2161 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     2221 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     2281 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     2341 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     2401 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     2461 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     2521 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     2581 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2641 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2701 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2761 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     2821 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2881 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     2941 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3001 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3061 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3181 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3241 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     3301 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3361 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     3421 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     3481 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     3541 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     3601 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     3661 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     3721 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     3781 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     3841 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     3901 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     3961 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4021 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4081 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     4141 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4201 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4261 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4321 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4381 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4441 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4501 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4561 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4621 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4681 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4741 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4801 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4861 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4921 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4981 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5041 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5101 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5161 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5221 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5281 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5341 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5401 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     5461 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5521 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5581 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5641 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5701 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5761 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5821 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5881 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5941 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6001 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6061 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6121 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6181 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6241 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6301 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     6361 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     6421 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     6481 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     6541 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     6601 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     6661 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     6721 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     6781 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     6841 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     6901 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     6961 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     7021 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     7081 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     7141 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7201 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7261 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7321 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     7381 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     7441 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     7501 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     7561 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     7621 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     7681 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     7741 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     7801 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     7861 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     7921 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     7981 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     8041 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     8101 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8161 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8221 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8281 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8341 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8401 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8461 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8521 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8581 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     8641 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     8701 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     8761 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     8821 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     8881 gagatccagt ttggttaat
//
