LOCUS       Exported                6460 bp ds-DNA     circular SYN 07-JUL-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDEST-hSTARR-luc-Pmyc-ccw
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 7, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
COMMENT     Clone 1 based on sequencing results
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        34..257
                     /label=MYC promoter
     intron          292..521
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     CDS             567..2219
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     protein_bind    2252..2376
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2417..2722)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(3042..3722)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     protein_bind    complement(3831..3955)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    4014..4135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4554..5142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5342..6202)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    6307..6355
                     /note="synthetic polyadenylation signal"
     misc_feature    6369..6460
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcccct agggtctgga cggctgagga cccccgagct
       61 gtgctgctcg cggccgccac cgccgggccc cggccgtccc tggctcccct cctgcctcga
      121 gaagggcagg gcttctcaga ggcttggcgg gaaaaagaac ggagggaggg atcgcgctga
      181 gtataaaagc cggttttcgg ggctttatct aactcgctgt agtaattcca gcgagaggca
      241 gagggagcga gcgggcgatt aattcgctgt ctgcgagggc cagctgttgg ggtgagtact
      301 ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa aacgaggagg
      361 atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc atctggtcag
      421 aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc tggccataca
      481 cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca
      541 actgcaggtc gcctgcaggc ttaagcatgg aagatgccaa aaacattaag aagggcccag
      601 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc
      661 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta
      721 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc
      781 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg
      841 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc
      901 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag
      961 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca
     1021 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt
     1081 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca
     1141 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc
     1201 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca
     1261 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca
     1321 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc
     1381 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat
     1441 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga
     1501 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc
     1561 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca
     1621 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta
     1681 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg
     1741 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca
     1801 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact
     1861 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag
     1921 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc
     1981 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa
     2041 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc
     2101 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg
     2161 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat
     2221 ctagagcatg caccggtgat agcttgatca aaccactttg tacaagaaag ctgaacgaga
     2281 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     2341 ataatactgt aaaacacaac atatccagtc actatggtcg acctgcagac tggctgtgta
     2401 taagggagcc tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat
     2461 tttcgcggtg gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca
     2521 tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac
     2581 gggggacttt atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg
     2641 gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat
     2701 aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcagcaaa agagccgttc
     2761 atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg
     2821 gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga gatattgaca
     2881 tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct
     2941 tcatagcata cctctttttg acatacttcg ggtatacata tcagtatata ttcttatacc
     3001 gcaaaaatca gcgcgcaaat acgcatactg ttatctggct tttagtaagc cggatcctct
     3061 agattacgcc ccgcctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     3121 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     3181 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     3241 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     3301 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     3361 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     3421 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     3481 tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg
     3541 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     3601 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     3661 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     3721 attttagctt ccttagctcc tgaaaatctc gacggatcct aactcaaaat ccacacatta
     3781 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgcggccgc catagtgact
     3841 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     3901 tattgatatt tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgttcgat
     3961 ctagaatggc tagatcctcg acgaattcgg ccggccgctt cgagcagaca tgataagata
     4021 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     4081 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     4141 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     4201 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgaccgatg cccttgagag
     4261 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     4321 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcagcg ctcttccgct
     4381 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4441 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4501 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4561 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4621 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4681 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4741 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4801 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4861 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4921 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4981 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5041 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5161 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5221 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5281 taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg
     5341 gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca
     5401 tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc
     5461 ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga
     5521 accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc
     5581 agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa
     5641 gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt
     5701 tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg
     5761 cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc
     5821 tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt
     5881 ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct
     5941 gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc
     6001 tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat
     6061 ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca
     6121 gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga
     6181 cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg
     6241 gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca
     6301 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     6361 atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag
     6421 gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//
