LOCUS       Exported                6325 bp ds-DNA     circular SYN 07-JUL-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDEST-hSTARR-luc-ccw
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 7, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
COMMENT     Clone3 picked based on sequencing result
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        34..114
                     /label=SCP1
     intron          157..386
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     CDS             432..2084
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     protein_bind    2117..2241
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2282..2587)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(2907..3587)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     protein_bind    complement(3696..3820)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    3879..4000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4419..5007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5207..6067)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    6172..6220
                     /note="synthetic polyadenylation signal"
     misc_feature    6234..6325
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcccct agggtactta tataaggggg tgggggcgcg
       61 ttcgtcctca gtcgcgatcg aacactcgag ccgagcagac gtgcctacgg accggggccc
      121 gaattaattc gctgtctgcg agggccagct gttggggtga gtactccctc tcaaaagcgg
      181 gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct
      241 ggcccgcggt gatgcctttg agggtggccg cgtccatctg gtcagaaaag acaatctttt
      301 tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc atacacttga gtgacaatga
      361 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc aggtcgcctg
      421 caggcttaag catggaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac
      481 tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac gccctggtgc
      541 ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact
      601 tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc
      661 ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt
      721 tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca
      781 gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc
      841 tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg
      901 actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca
      961 acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga
     1021 acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg
     1081 tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta
     1141 tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga
     1201 tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct
     1261 tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc ttcttcgcta
     1321 agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg
     1381 cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc
     1441 gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg
     1501 acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg
     1561 acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga
     1621 tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac aaggacggct
     1681 ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc
     1741 ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca
     1801 tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg
     1861 ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg
     1921 agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg
     1981 tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg
     2041 agattctcat taaggccaag aagggcggca agatcgccgt gtaatctaga gcatgcaccg
     2101 gtgatagctt gatcaaacca ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat
     2161 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2221 acaacatatc cagtcactat ggtcgacctg cagactggct gtgtataagg gagcctgaca
     2281 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
     2341 gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca
     2401 tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacggggg actttatctg
     2461 acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat
     2521 cactctgtac atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa
     2581 actgcatttc accagcccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg
     2641 gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg
     2701 gcttcattct gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga
     2761 gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcatacctct
     2821 ttttgacata cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg
     2881 caaatacgca tactgttatc tggcttttag taagccggat cctctagatt acgccccgcc
     2941 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     3001 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     3061 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     3121 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     3181 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     3241 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     3301 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     3361 gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     3421 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     3481 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     3541 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     3601 gctcctgaaa atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca
     3661 taaagtgtaa agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt
     3721 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
     3781 cattttacgt ttctcgttca gcttttttgt acaaacttgt tcgatctaga atggctagat
     3841 cctcgacgaa ttcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
     3901 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     3961 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4021 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     4081 caaatgtggt aaaatcgata aggatccgac cgatgccctt gagagccttc aacccagtca
     4141 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     4201 tcatgcaact cgtaggacag gtgccggcag cagcgctctt ccgcttcctc gctcactgac
     4261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5161 taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga
     5221 tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc
     5281 ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga
     5341 tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga
     5401 gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct
     5461 gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg
     5521 ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc
     5581 agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag
     5641 ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag
     5701 cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt
     5761 actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt
     5821 ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc
     5881 gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc
     5941 ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg
     6001 caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga
     6061 tgctcatact cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct
     6121 ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata
     6181 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
     6241 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
     6301 agtgcaggtg ccagaacatt tctct
//
