LOCUS       64 - pDOC-K-glmS-GFPrev final        8894 bp    DNA     circular UNA 10-SEP-2020
DEFINITION  166,944 reads from PID-0299-Elef-64_S143_R_001 mapped to 166,978
            locations in 64 using Geneious.
ACCESSION   urn.local...54-bg87rla
VERSION     urn.local...54-bg87rla
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        96..200
     CDS             201..1061
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     primer_bind     complement(419..438)
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp-R"
     rep_origin      1232..1819
     primer_bind     1720..1739
                     /note="pBR322 origin, forward primer"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pBR322ori-F"
     primer_bind     1973..1990
                     /note="L4440 vector, forward primer"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="L4440"
     protein_bind    2107..2128
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP binding site"
     promoter        2143..2173
     protein_bind    2181..2197
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lac operator"
     primer_bind     complement(2199..2217)
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lac I rev (98)"
     primer_bind     2205..2221
                     /note="In lacZ gene. Also called M13-rev"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="M13 Reverse"
     primer_bind     2222..2242
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pDoC-K For"
     misc_feature    2263..2692
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="glms_part1"
     primer_bind     complement(2684..2703)
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="glms1-promoter Rev"
     primer_bind     2710..2727
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="glms1-promoter For"
     protein_bind    complement(2724..2771)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="FRT"
     CDS             2981..3775
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418
                      (Geneticin(R))"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="NeoR/KanR"
     primer_bind     complement(3035..3054)
                     /note="Neomycin resistance gene, reverse primer"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Neo-R"
     primer_bind     3645..3664
                     /note="Neomycin resistance gene, forward primer"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Neo-F"
     primer_bind     3732..3753
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Kan_test_For"
     protein_bind    complement(3966..3999)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="FRT (minimal)"
     primer_bind     complement(4014..4034)
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pDOCK-glms-XhoI Rev"
     primer_bind     complement(4015..4041)
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pdock-glms-NdeI-Rev"
     CDS             complement(4044..4760)
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV
                     light"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="GFPuv"
     primer_bind     4044..4065
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="eGFP-Rev (puc18-egfp)"
     primer_bind     4762..4802
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Vector For"
     misc_feature    complement(4812..4838)
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="acpp promoter"
     primer_bind     4839..4857
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Vector Rev"
     primer_bind     4850..4866
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pDOCK-glms-Hindiii-For"
     misc_feature    4856..5275
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="glms_part2"
     primer_bind     complement(4876..4895)
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="glms_test_Rev"
     primer_bind     complement(5318..5335)
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="M13 Forward"
     primer_bind     complement(5336..5356)
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pDocK-Rev"
     primer_bind     complement(5514..5531)
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pBAD Reverse"
     terminator      5684..5770
     terminator      5862..5889
     oriT            6336..6445
                     /note="incP origin of transfer"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="incP origin of transfer oriT"
     promoter        6819..7264
     CDS             7265..8686
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /note="negative selection marker"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SacB"
     primer_bind     complement(7697..7716)
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SacB test reverse"
     primer_bind     complement(8725..8744)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pRS-marker"
     primer_bind     8844..8866
                     /note="pGEX vectors, reverse primer"
                     /note="Derived using Geneious Prime 2020.2.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pGEX 3'"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta gccaccactt caagaactct gtagcaccgc ctacatacct
     1441 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1501 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1561 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1621 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1681 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1741 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1801 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1861 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1921 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1981 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2041 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2101 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2161 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2221 ccatgattac gccaagctct agggataaca gggtaatcga tgaattcgct cgaaggcgcg
     2281 ctgaagctga aagagatctc ttacattcac gcggaagcct atgctgcagg cgagctgaaa
     2341 cacgggccgt tggcgctgat tgacgccgat atgccggtga ttgtggtggc gccgaacaac
     2401 gaactgctgg agaaactgaa atccaacatc gaagaagttc gcgcacgtgg cggtcagttg
     2461 tacgtcttcg ccgatcagga tgcgggtttc gtcagcaacg acaacatgca catcatcgag
     2521 atgccgcatg tggaagaggt cattgcgcct atcttctaca ccgttccgct gcaactgctg
     2581 gcgtatcacg tggcgctgat taaaggcacc gacgttgacc agccgcgtaa cctggcgaaa
     2641 tcggttaccg tagagtaagt acaaatgtag taccaggcag aaggccgggt acctaggacc
     2701 ggtcaattgg ctggagctgc ttcgaagttc ctatactttc tagagaatag gaacttcgga
     2761 ataggaactt caagatcccc cacgctgccg caagcactca gggcgcaagg gctgctaaag
     2821 gaagcggaac acgtagaact taagggaatt gccagctggg gcgccctctg gtaaggttgg
     2881 gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg
     2941 atcaagatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     3001 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     3061 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     3121 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     3181 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     3241 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     3301 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     3361 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     3421 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     3481 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     3541 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     3601 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     3661 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgctct acggtatcgc
     3721 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     3781 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     3841 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     3901 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccag cttcaaaagc
     3961 gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac taaggaggat
     4021 attcatatct cgagcatatg cccttatttg tagagctcat ccatgccatg tgtaatccca
     4081 gcagcagtta caaactcaag aaggaccatg tggtcacgct tttcgttggg atctttcgaa
     4141 agggcagatt gtgtcgacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt
     4201 ggagtatttt gttgataatg gtctgctagt tgaacggatc catcttcaat gttgtggcga
     4261 attttgaagt tagctttgat tccattcttt tgtttgtctg ccgtgatgta tacattgtgt
     4321 gagttatagt tgtactcgag tttgtgtccg agaatgtttc catcttcttt aaaatcaata
     4381 ccttttaact cgatacgatt aacaagggta tcaccttcaa acttgacttc agcacgcgtc
     4441 ttgtagttcc cgtcatcttt gaaagatata gtgcgttcct gtacataacc ttcgggcatg
     4501 gcactcttga aaaagtcatg ccgtttcata tgatccggat aacgggaaaa gcattgaaca
     4561 ccataggtca aagtagtgac aagtgttggc catggaacag gtagttttcc agtagtgcaa
     4621 ataaatttaa gggtaagctt tccgtatgta gcatcacctt caccctctcc actgacagaa
     4681 aatttgtgcc cattaacatc accatctaat tcaacaagaa ttgggacaac tccagtgaaa
     4741 agttcttctc ctttactcat atgtatatct ccttcttaaa tccaaaactc gctttcgcga
     4801 tggttttcgt agtgtataaa atgttgaaaa atttgcaagg ggcggccgca agctttcgac
     4861 agacggcctt tttttgtgcg ctgtgacagg cgctgttctt tctctatggt ggctgaagag
     4921 cgactaagag gtgctacgcc tgcctgcgct acgggcaccc ggtcacattc tcttctttaa
     4981 ggccctggct caatccaggt ggatcggaaa tcaaaccacc ccaggttatt caggtggatc
     5041 cccctggccc ggtcggggct tttgagtcgc atccagtggt gaaaaagcgg ctgtagccag
     5101 cctgaacggg tggtttccct gaccagttgt tccgcgctga tggtttcggc atgccactgg
     5161 gtttcccatt gggccagcag cgcatcatcc ccaccactga tggcgtggcg cagtaaaggg
     5221 gagcccagta gccatgtacc gacgttccac tcttcgggaa tggggaaatt gaccggctag
     5281 cgtcgactag tagggataac agggtaatga gcttggcact ggccgtcgtt ttacaacgtc
     5341 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5401 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5461 tgaatggcga atggcgagct tggctgtttt ggcggatgag agaagatttt cagcctgata
     5521 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc
     5581 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt
     5641 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
     5701 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
     5761 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg
     5821 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga
     5881 tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgc
     5941 atgcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatcg
     6001 acatccgccc tcaccgccag gaacgcaacc gcagcctcat cacgccggcg cttcttggcc
     6061 gcgcgggatt caacccactc ggccagctcg tcggtgtagc tctttggcat cgtctctcgc
     6121 ctgtcccctc agttcagtaa tttcctgcat ttgcctgttt ccagtcggta gatattccac
     6181 aaaacagcag ggaagcagcg cttttccgct gcataaccct gcttcggggt cattatagcg
     6241 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     6301 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
     6361 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     6421 aatcctgctc tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg
     6481 gctgatgaaa ccaagccaac caggaagggc agcccaccta tcaaggtgta ctgccttcca
     6541 gacgaacgaa gagcgattga ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac
     6601 ctgctggccg tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc
     6661 gagctggccc gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc
     6721 accgacgacc cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag
     6781 atcgactcta gctagaggat cgatcctttt taacccatca catatacctg ccgttcacta
     6841 ttatttagtg aaatgagata ttatgatatt ttctgaattg tgattaaaaa ggcaacttta
     6901 tgcccatgca acagaaacta taaaaaatac agagaatgaa aagaaacaga tagatttttt
     6961 agttctttag gcccgtagtc tgcaaatcct tttatgattt tctatcaaac aaaagaggaa
     7021 aatagaccag ttgcaatcca aacgagagtc taatagaatg aggtcgaaaa gtaaatcgcg
     7081 cgggtttgtt actgataaag caggcaagac ctaaaatgtg taaagggcaa agtgtatact
     7141 ttggcgtcac cccttacata ttttaggtct ttttttattg tgcgtaacta acttgccatc
     7201 ttcaaacagg agggctggaa gaagcagacc gctaacacag tacataaaaa aggagacatg
     7261 aacgatgaac atcaaaaagt ttgcaaaaca agcaacagta ttaaccttta ctaccgcact
     7321 gctggcagga ggcgcaactc aagcgtttgc gaaagaaacg aaccaaaagc catataagga
     7381 aacatacggc atttcccata ttacacgcca tgatatgctg caaatccctg aacagcaaaa
     7441 aaatgaaaaa tatcaagttc ctgagttcga ttcgtccaca attaaaaata tctcttctgc
     7501 aaaaggcctg gacgtttggg acagctggcc attacaaaac gctgacggca ctgtcgcaaa
     7561 ctatcacggc taccacatcg tctttgcatt agccggagat cctaaaaatg cggatgacac
     7621 atcgatttac atgttctatc aaaaagtcgg cgaaacttct attgacagct ggaaaaacgc
     7681 tggccgcgtc tttaaagaca gcgacaaatt cgatgcaaat gattctatcc taaaagacca
     7741 aacacaagaa tggtcaggtt cagccacatt tacatctgac ggaaaaatcc gtttattcta
     7801 cactgatttc tccggtaaac attacggcaa acaaacactg acaactgcac aagttaacgt
     7861 atcagcatca gacagctctt tgaacatcaa cggtgtagag gattataaat caatctttga
     7921 cggtgacgga aaaacgtatc aaaatgtaca gcagttcatc gatgaaggca actacagctc
     7981 aggcgacaac catacgctga gagatcctca ctacgtagaa gataaaggcc acaaatactt
     8041 agtatttgaa gcaaacactg gaactgaaga tggctaccaa ggcgaagaat ctttatttaa
     8101 caaagcatac tatggcaaaa gcacatcatt cttccgtcaa gaaagtcaaa aacttctgca
     8161 aagcgataaa aaacgcacgg ctgagttagc aaacggcgct ctcggtatga ttgagctaaa
     8221 cgatgattac acactgaaaa aagtgatgaa accgctgatt gcatctaaca cagtaacaga
     8281 tgaaattgaa cgcgcgaacg tctttaaaat gaacggcaaa tggtatctgt tcactgactc
     8341 ccgcggatca aaaatgacga ttgacggcat tacgtctaac gatatttaca tgcttggtta
     8401 tgtttctaat tctttaactg gcccatacaa gccgctgaac aaaactggcc ttgtgttaaa
     8461 aatggatctt gatcctaacg atgtaacctt tacttactca cacttcgctg tacctcaagc
     8521 gaaaggaaac aatgtcgtga ttacaagcta tatgacaaac agaggattct acgcagacaa
     8581 acaatcaacg tttgcgccta gcttcctgct gaacatcaaa ggcaagaaaa catctgttgt
     8641 caaagacagc atccttgaac aaggacaatt aacagttaac aaataaaaac gcaaaagaaa
     8701 atgccgatta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     8761 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     8821 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     8881 accgaaacgc gcga
//
