LOCUS       Exported               15380 bp ds-DNA     circular SYN 07-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15380)
  AUTHORS   qqe
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 10, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15380
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    139..160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        175..205
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    213..229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     237..253
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    271..943
                     /label=UBI10 promoter
     misc_feature    944..1022
                     /label=exon1
     misc_feature    1023..2004
                     /label=modified intron
     misc_feature    2005..2006
                     /label=exon2 start
     protein_bind    2020..2044
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2089..2154
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2161..2181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2206..6306
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6307..6354
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    complement(6387..6411)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6422..6445
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      6467..6719
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6728..6744)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6745..6761)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6964..6988
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8288..8917
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9351..10418
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    11022..11162
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11348..11936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12023..12817)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13242..13266
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13344..13518
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(13558..14583)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(14651..15328)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcctctccc
       61 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      121 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      181 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      241 aaacagctat gacatgatta cgccaagctt acaaattcgg gtcaaggcgg aagccagcgc
      301 gccaccccac gtcagcaaat acggaggcgc ggggttgacg gcgtcacccg gtcctaacgg
      361 cgaccaacaa accagccaga agaaattaca gtaaaaaaaa agtaaattgc actttgatcc
      421 accttttatt acctaagtct caatttggat cacccttaaa cctatctttt caatttgggc
      481 cgggttgtgg tttggactac catgaacaac ttttcgtcat gtctaacttc cctttcagca
      541 aacatatgaa ccatatatag aggagatcgg ccgtatacta gagctgatgt gtttaaggtc
      601 gttgattgca cgagaaaaaa aaatccaaat cgcaacaata gcaaatttat ctggttcaaa
      661 gtgaaaagat atgtttaaag gtagtccaaa gtaaaactta tagataataa aatgtggtcc
      721 aaagcgtaat tcactcaaaa aaaatcaacg agacgtgtac caaacggaga caaacggcat
      781 cttctcgaaa tttcccaacc gctcgctcgc ccgcctcgtc ttcccggaaa ccgcggtggt
      841 ttcagcgtgg cggattctcc aagcagacgg agacgtcacg gcacgggact cctcccacca
      901 cccaaccgcc ataaatacca gccccctcat ctcctctcct cgcatcagct ccacccccga
      961 aaaatttctc cccaatctcg cgaggctctc gtcgtcgaat cgaatcctct cgcgtcctca
     1021 aggtacgctg cttctcctct cctcgcttcg tttcgattcg atttcggacg ggtgaggttg
     1081 ttttgttgct agatccgatt ggtggttagg gttgtcgatg tgattatcgt gagatgttta
     1141 ggggttgtag atctgatggt tgtgatttgg gcacggttgg ttcgataggt ggaatcgtgg
     1201 ttaggttttg ggattggatg ttggttctga tgattggggg gaatttttac ggttagatga
     1261 attgttggat gattcgattg gggaaatcgg tgtagatctg ttggggaatt gtggaactag
     1321 tcatgcctga gtgattggtg cgatttgtag cgtgttccat cttgtaggcc ttgttgcgag
     1381 catgttcaga tctactgttc cgctcttgat tgagttattg gtgccatggg ttggtgcaaa
     1441 cacaggcttt aatatgttat atctgttttg tgtttgatgt agatctgtag ggtagttctt
     1501 cttagacatg gttcaattat gtagcttgtg cgtttcgatt tgatttcata tgttcacaga
     1561 ttagataatg atgaactctt ttaattaatt gtcaatggta aataggaagt cttgtcgcta
     1621 tatctgtcat aatgatctca tgttactatc tgccagtaat ttatgctaag aactatatta
     1681 gaatatcatg ttacaatctg tagtaatatc atgttacaat ctgtagttca tctatataat
     1741 ctattgtggt aatttctttt tactatctgt gtgaagatta ttgccactag ttcattctac
     1801 ttatttctga agttcaggat acgtgtgctg ttactaccta tctgaataca tgtgtgatgt
     1861 gcctgttact atctttttga atacatgtat gttctgttgg aatatggcag gagaccgagg
     1921 tctcggtttg ctgtttgatc cgttgttgtg tccttaatct tgtgctagtt cttaccctat
     1981 ctgtttggtg attatttctt gcagattcta gagttatcaa caagtttgta caaaaaagca
     2041 ggcttcgaag gagatagaac caattctcta aggaaatact taaccatgga ctataaggac
     2101 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc
     2161 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc
     2221 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     2281 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     2341 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     2401 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     2461 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     2521 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     2581 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     2641 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     2701 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     2761 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     2821 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     2881 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     2941 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     3001 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     3061 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     3121 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     3181 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     3241 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     3301 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     3361 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     3421 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     3481 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     3541 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     3601 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     3661 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     3721 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     3781 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     3841 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     3901 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     3961 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     4021 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     4081 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     4141 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     4201 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     4261 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     4321 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     4381 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     4441 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     4501 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     4561 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     4621 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     4681 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     4741 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     4801 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     4861 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     4921 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     4981 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     5041 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     5101 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     5161 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     5221 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     5281 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     5341 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     5401 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     5461 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     5521 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     5581 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     5641 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     5701 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     5761 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     5821 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     5881 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     5941 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     6001 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     6061 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     6121 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     6181 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     6241 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     6301 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagtaagaa
     6361 ttcgcggccg cactcgagat atctagaccc agctttcttg tacaaagtgg ttgataacag
     6421 cgactacaag gatgacgatg acaaggctta gagctcgaat ttccccgatc gttcaaacat
     6481 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     6541 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     6601 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     6661 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     6721 ggaattcact ggccgtcgtt ttacactggc cgtcgtttta caacgtcgtg actgggaaaa
     6781 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6841 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6901 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
     6961 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataacgg
     7021 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     7081 cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc
     7141 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
     7201 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
     7261 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
     7321 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
     7381 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
     7441 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
     7501 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
     7561 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
     7621 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
     7681 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
     7741 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
     7801 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
     7861 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
     7921 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
     7981 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
     8041 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc
     8101 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
     8161 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
     8221 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
     8281 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
     8341 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
     8401 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
     8461 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
     8521 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
     8581 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
     8641 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
     8701 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
     8761 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
     8821 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
     8881 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
     8941 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
     9001 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
     9061 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
     9121 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
     9181 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
     9241 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg
     9301 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac
     9361 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     9421 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     9481 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
     9541 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     9601 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     9661 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     9721 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     9781 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     9841 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
     9901 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
     9961 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
    10021 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
    10081 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
    10141 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
    10201 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
    10261 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
    10321 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
    10381 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
    10441 aaattgccct agcaggggaa aaaggtcgaa aagcactctt tcctgtggat agcacgtaca
    10501 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    10561 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    10621 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    10681 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
    10741 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
    10801 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
    10861 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
    10921 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    10981 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
    11041 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
    11101 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    11161 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    11221 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    11281 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    11341 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    11401 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    11461 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    11521 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    11581 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    11641 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    11701 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    11761 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    11821 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    11881 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    11941 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    12001 ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa tattttattt
    12061 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
    12121 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
    12181 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
    12241 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
    12301 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
    12361 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
    12421 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
    12481 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
    12541 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
    12601 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
    12661 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
    12721 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
    12781 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
    12841 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
    12901 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
    12961 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
    13021 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
    13081 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
    13141 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
    13201 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
    13261 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga
    13321 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
    13381 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
    13441 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
    13501 cacattatta tggagaaact cgagcttgtc gatcgacaga tccggtcggc atctactcta
    13561 tttctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
    13621 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
    13681 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
    13741 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
    13801 tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
    13861 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
    13921 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
    13981 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
    14041 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
    14101 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
    14161 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
    14221 cgcatccata gcctccgcga ccggttgtag aacagcgggc agttcggttt caggcaggtc
    14281 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctaaactc
    14341 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
    14401 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
    14461 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
    14521 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
    14581 catatctcat tgccccccgg gatctgcgaa agctcgagag agatagattt gtagagagag
    14641 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaaggtc
    14701 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
    14761 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
    14821 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
    14881 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
    14941 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
    15001 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
    15061 gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
    15121 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
    15181 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
    15241 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
    15301 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
    15361 gatattcttg gagtagacga
//
