LOCUS       Exported               15159 bp ds-DNA     circular SYN 07-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15159)
  AUTHORS   qqe
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 10, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15159
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    44..65
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     52..72
                     /label=174 Seq-1300-F
     promoter        80..110
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    118..134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     142..158
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    176..848
                     /label=UBI10 promoter
     misc_feature    849..927
                     /label=exon 1
     primer_bind     893..914
                     /label=exon1-qF
     misc_feature    928..1909
                     /label=modified intron
     primer_bind     1725..1749
                     /label=217 RGEB33-seqF
     primer_bind     complement(1888..1917)
                     /label=89 UBI-in-R
     misc_feature    1910..1911
                     /label=exon2 start
     protein_bind    1925..1949
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1944..1966)
                     /label=33-R
     primer_bind     complement(1973..2015)
                     /label=FnCpf1-GR
     misc_feature    1994..6058
                     /label=OsFnCpf1
     CDS             1994..2059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2066..2086
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2083..2102)
                     /label=33Fn-qR
     primer_bind     2118..2137
                     /label=549 Cpf1-F
     primer_bind     complement(2404..2423)
                     /label=538 FnCpf1-R
     primer_bind     2822..2841
                     /label=1234 Fn-F
     primer_bind     complement(3394..3413)
                     /label=659 Fn-1R
     primer_bind     complement(3578..3597)
                     /label=1235 Fn-R
     primer_bind     3865..3884
                     /label=1292 Fn-3F
     primer_bind     complement(4078..4097)
                     /label=660 Fn-2R
     primer_bind     4674..4693
                     /label=619 FnDtoA-F
     primer_bind     complement(5058..5077)
                     /label=1293 Fn-3R
     primer_bind     complement(5843..5862)
                     /label=Fn-4R
     CDS             6008..6055
                     /codon_start=1
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    6059..6084
                     /label=MCS 2
                     /note="multiple cloning site, part 2"
     protein_bind    complement(6088..6112)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6123..6146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      6168..6420
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6429..6445)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6429..6445)
                     /label=M13-F
     misc_feature    6648..6672
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7972..8601
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9035..10102
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    10706..10846
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11032..11620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11707..12501)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12926..12950
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13028..13202
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(13242..14267)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(14335..15012)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
       61 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      121 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgcca agcttacaaa
      181 ttcgggtcaa ggcggaagcc agcgcgccac cccacgtcag caaatacgga ggcgcggggt
      241 tgacggcgtc acccggtcct aacggcgacc aacaaaccag ccagaagaaa ttacagtaaa
      301 aaaaaagtaa attgcacttt gatccacctt ttattaccta agtctcaatt tggatcaccc
      361 ttaaacctat cttttcaatt tgggccgggt tgtggtttgg actaccatga acaacttttc
      421 gtcatgtcta acttcccttt cagcaaacat atgaaccata tatagaggag atcggccgta
      481 tactagagct gatgtgttta aggtcgttga ttgcacgaga aaaaaaaatc caaatcgcaa
      541 caatagcaaa tttatctggt tcaaagtgaa aagatatgtt taaaggtagt ccaaagtaaa
      601 acttatagat aataaaatgt ggtccaaagc gtaattcact caaaaaaaat caacgagacg
      661 tgtaccaaac ggagacaaac ggcatcttct cgaaatttcc caaccgctcg ctcgcccgcc
      721 tcgtcttccc ggaaaccgcg gtggtttcag cgtggcggat tctccaagca gacggagacg
      781 tcacggcacg ggactcctcc caccacccaa ccgccataaa taccagcccc ctcatctcct
      841 ctcctcgcat cagctccacc cccgaaaaat ttctccccaa tctcgcgagg ctctcgtcgt
      901 cgaatcgaat cctctcgcgt cctcaaggta cgctgcttct cctctcctcg cttcgtttcg
      961 attcgatttc ggacgggtga ggttgttttg ttgctagatc cgattggtgg ttagggttgt
     1021 cgatgtgatt atcgtgagat gtttaggggt tgtagatctg atggttgtga tttgggcacg
     1081 gttggttcga taggtggaat cgtggttagg ttttgggatt ggatgttggt tctgatgatt
     1141 ggggggaatt tttacggtta gatgaattgt tggatgattc gattggggaa atcggtgtag
     1201 atctgttggg gaattgtgga actagtcatg cctgagtgat tggtgcgatt tgtagcgtgt
     1261 tccatcttgt aggccttgtt gcgagcatgt tcagatctac tgttccgctc ttgattgagt
     1321 tattggtgcc atgggttggt gcaaacacag gctttaatat gttatatctg ttttgtgttt
     1381 gatgtagatc tgtagggtag ttcttcttag acatggttca attatgtagc ttgtgcgttt
     1441 cgatttgatt tcatatgttc acagattaga taatgatgaa ctcttttaat taattgtcaa
     1501 tggtaaatag gaagtcttgt cgctatatct gtcataatga tctcatgtta ctatctgcca
     1561 gtaatttatg ctaagaacta tattagaata tcatgttaca atctgtagta atatcatgtt
     1621 acaatctgta gttcatctat ataatctatt gtggtaattt ctttttacta tctgtgtgaa
     1681 gattattgcc actagttcat tctacttatt tctgaagttc aggatacgtg tgctgttact
     1741 acctatctga atacatgtgt gatgtgcctg ttactatctt tttgaataca tgtatgttct
     1801 gttggaatat ggcaggagac cgaggtctcg gtttgctgtt tgatccgttg ttgtgtcctt
     1861 aatcttgtgc tagttcttac cctatctgtt tggtgattat ttcttgcaga ttctagagtt
     1921 atcaacaagt ttgtacaaaa aagcaggctt cgaaggagat agaaccaatt ctctaaggaa
     1981 atacttaacc atggattata aggatcatga tggagattac aaggatcacg atattgatta
     2041 taaggatgat gatgataaga tggctcctaa gaagaagaga aaggttggca ttcatggtgt
     2101 gccggctgcc tctatctacc aggagttcgt taataagtac tctttgtcaa agaccctcag
     2161 gtttgagctt attcctcaag gcaagacttt ggaaaacatc aaggccagag gtcttatttt
     2221 ggatgatgag aagagggcta aggattacaa gaaggccaag cagatcattg ataagtacca
     2281 ccaattcttt atcgaggaaa ttctttcttc agtgtgcatc tcagaagatc tccttcagaa
     2341 ttactctgat gtctatttca agttgaagaa gtcagatgat gataacctcc agaaggattt
     2401 taagtctgct aaggatacta tcaagaagca aatttcagag tacatcaagg attctgaaaa
     2461 gtttaagaat ttgttcaacc agaatctcat tgatgccaag aagggccaag agtcagatct
     2521 catcctttgg ttgaagcaat ctaaggataa tggtatcgaa cttttcaagg ctaactcaga
     2581 tatcacagat attgatgagg ccttggaaat cattaagtct tttaagggct ggactacata
     2641 ttttaagggt ttccatgaga acagaaagaa tgtctactct tcaaacgata ttcctacttc
     2701 tatcatctac aggatcgttg atgataattt gccaaagttc ctcgagaaca aggctaagta
     2761 cgaatctctc aaggataagg ctccagaggc cattaattac gaacagatca agaaggatct
     2821 tgccgaggaa ttgacattcg atattgatta caagacctca gaggttaacc agagagtgtt
     2881 ttctttggat gaggtgttcg aaatcgctaa cttcaacaat tatctcaacc aatcaggcat
     2941 taccaagttc aatactatca ttggtggcaa gtttgtcaac ggagagaata caaagaggaa
     3001 gggcattaac gaatacatca atttgtactc tcagcaaatt aacgataaga cactcaagaa
     3061 gtacaagatg tcagtgcttt tcaagcagat cttgtctgat accgagtcta agtcatttgt
     3121 cattgataag ctcgaagatg attcagatgt tgtgaccact atgcagtctt tttacgagca
     3181 aatcgctgcc ttcaagacag ttgaggaaaa gtcaattaag gaaacccttt ctttgctctt
     3241 cgatgatttg aaggctcaaa agctcgatct ttcaaagatc tatttcaaga acgataagtc
     3301 acttaccgat ttgtctcagc aagtgtttga tgattactct gtcattggta ctgctgttct
     3361 tgagtatatt acacagcaaa tcgccccgaa gaaccttgat aatccttcaa agaaggagca
     3421 ggaattgatc gctaagaaga ctgagaaggc caagtacttg tctctcgaaa ctatcaagct
     3481 cgctttggag gagttcaaca agcatagaga tattgataag caatgcaggt ttgaggaaat
     3541 cctcgccaac ttcgctgcca tcccgatgat ttttgatgag atcgctcaga acaaggataa
     3601 tcttgcccaa atctcaatta agtatcagaa ccaaggaaag aaggatcttt tgcaggcttc
     3661 tgccgaagat gatgttaagg ctattaagga tctccttgat cagaccaaca atttgctcca
     3721 taagctcaag atcttccaca tttctcaatc agaggataag gctaacatcc ttgataagga
     3781 tgaacacttc tacttggttt tcgaggaatg ttatttcgag cttgccaaca ttgtgccgtt
     3841 gtacaacaag atcagaaact acatcactca gaagccttac tcagatgaga agtttaagct
     3901 caacttcgaa aattctaccc ttgctaacgg ttgggataag aataaggagc cagataacac
     3961 tgccatcctc ttcattaagg atgataagta ctatcttgga gtgatgaata agaagaacaa
     4021 taagatcttc gatgataagg ctattaagga gaacaagggc gaaggttaca agaagatcgt
     4081 ttacaagctc ttgcctggag ctaataagat gttgccaaag gtgttctttt cagccaagtc
     4141 tattaagttc tacaacccat ctgaggatat cctcagaatt aggaatcatt caacacacac
     4201 caagaacgga tctccgcaga agggctacga gaagtttgag ttcaacatcg aggattgcag
     4261 aaagtttatt gatttctata agcaatctat ctcaaagcat cctgagtgga aggattttgg
     4321 cttcagattt tcagatactc agaggtacaa ctctattgat gagttctata gggaggttga
     4381 aaatcaaggt tacaagctca cattcgagaa catctctgaa tcatacattg attctgtcgt
     4441 taaccaggga aagctctacc ttttccaaat ctacaacaag gatttttcag cttactctaa
     4501 gggcagacca aatctccaca ctctttattg gaaggccctc ttcgatgaga ggaatcttca
     4561 ggatgtggtc tacaagttga acggagaggc tgaactcttt tatagaaagc aatcaattcc
     4621 aaagaagatc acacatccgg ccaaggaggc tatcgccaac aagaataagg ataatcctaa
     4681 gaaggagtct gtgttcgaat acgatcttat taaggataag aggtttacag aggataagtt
     4741 ctttttccac tgtccaatca ccattaactt caagtcttca ggcgctaaca agtttaatga
     4801 tgagatcaat ctccttttga aggaaaaggc caacgatgtt catatcctct ctattgatag
     4861 aggagagagg cacctcgctt actatacact tgtggatgga aagggcaata tcattaagca
     4921 ggatactttc aacatcattg gcaatgatag gatgaagaca aactaccatg ataagctcgc
     4981 tgccattgaa aaggatagag attcagccag gaaggattgg aagaagatca acaatattaa
     5041 ggagatgaag gaaggctacc tctctcaagt tgtgcacgag attgctaagc tcgttatcga
     5101 atataatgcc attgtcgttt tcgaggatct caatttcggt tttaagagag gaaggtttaa
     5161 ggtcgaaaag caggtttacc aaaagctcga gaagatgctt atcgaaaagt tgaactatct
     5221 cgtctttaag gataacgagt tcgataagac cggtggagtt ttgagggctt accagctcac
     5281 agccccgttt gaaaccttca agaagatggg caagcaaact ggaatcatct actatgtccc
     5341 ggctggattc acctctaaga tctgccctgt gactggcttt gtcaatcagc tctaccctaa
     5401 gtatgagtct gtttcaaagt ctcaagaatt tttctcaaag ttcgataaga tctgttacaa
     5461 tcttgataag ggttacttcg agttttcttt cgattacaag aacttcggag ataaggctgc
     5521 caagggaaag tggactattg cttcattcgg atctagactt atcaacttca gaaactctga
     5581 taagaaccat aactgggata ccagagaggt ctatccaact aaggagttgg aaaagctcct
     5641 taaggattac tctatcgagt atggtcacgg agaatgcatt aaggctgcca tctgtggaga
     5701 gtcagataag aagtttttcg ctaagttgac ttctgtgctc aatacaatcc ttcagatgag
     5761 aaactcaaag actggtacag aacttgatta cttgatttct ccggtggctg atgtcaacgg
     5821 aaacttcttc gattctaggc aggcccctaa gaatatgcca caagatgctg atgccaacgg
     5881 cgcctaccac atcggactca agggccttat gttgctcggt aggattaaga acaatcaaga
     5941 gggaaagaag ttgaatctcg tgattaagaa cgaggaatat tttgagttcg tccagaacag
     6001 aaacaataag aggcctgctg ccacaaagaa ggctggtcaa gctaagaaga agaagtgaga
     6061 attcgcggcc gcactcgaga tatctagacc cagctttctt gtacaaagtg gttgataaca
     6121 gcgactacaa ggatgacgat gacaaggctt agagctcgaa tttccccgat cgttcaaaca
     6181 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     6241 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     6301 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     6361 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     6421 gggaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6481 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6541 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc
     6601 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     6661 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt
     6721 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     6781 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     6841 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     6901 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     6961 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     7021 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     7081 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     7141 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     7201 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     7261 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     7321 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     7381 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
     7441 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     7501 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     7561 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     7621 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     7681 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     7741 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     7801 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     7861 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     7921 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     7981 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     8041 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     8101 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     8161 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     8221 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     8281 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     8341 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     8401 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     8461 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     8521 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     8581 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     8641 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     8701 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     8761 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     8821 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     8881 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     8941 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
     9001 actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca
     9061 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     9121 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     9181 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     9241 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     9301 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     9361 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     9421 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     9481 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     9541 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     9601 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     9661 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     9721 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     9781 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     9841 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     9901 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     9961 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
    10021 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
    10081 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
    10141 ggaaaaaggt cgaaaagcac tctttcctgt ggatagcacg tacattggga acccaaagcc
    10201 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
    10261 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
    10321 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
    10381 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
    10441 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
    10501 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
    10561 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10621 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10681 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
    10741 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10801 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
    10861 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10921 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10981 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    11041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11221 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    11341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    11401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    11461 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    11521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11641 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc
    11701 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    11761 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    11821 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    11881 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    11941 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    12001 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    12061 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    12121 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    12181 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    12241 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    12301 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    12361 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    12421 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    12481 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    12541 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    12601 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    12661 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
    12721 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
    12781 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
    12841 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
    12901 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
    12961 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
    13021 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
    13081 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
    13141 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
    13201 aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt tgccctcgga
    13261 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
    13321 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
    13381 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
    13441 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca
    13501 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
    13561 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
    13621 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
    13681 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
    13741 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
    13801 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
    13861 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc
    13921 gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
    13981 tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact
    14041 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
    14101 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
    14161 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
    14221 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc
    14281 ccgggatctg cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg
    14341 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg
    14401 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
    14461 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
    14521 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    14581 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
    14641 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
    14701 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtta
    14761 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
    14821 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
    14881 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
    14941 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
    15001 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
    15061 acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc
    15121 tccccgcgcg ttggccgatt cattaatgca gctggcacg
//
