LOCUS       Exported               14945 bp ds-DNA     circular SYN 07-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14945)
  AUTHORS   qqe
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 10, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14945
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    198..219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        234..264
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    272..288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     296..312
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    330..1002
                     /label=UBI10 promoter
     misc_feature    1003..1081
                     /label=exon 1
     intron          1082..2063
                     /label=modified intron
     misc_feature    2064..2065
                     /label=exon2 start
     protein_bind    2079..2103
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2160..5981
                     /label=hLbCpf1
     CDS             5844..5891
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5898..5924
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5925..5951
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5952..5978
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6011..6035)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6046..6069
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      6091..6343
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6352..6368)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6369..6385)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6588..6612
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7912..8541
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8975..10042
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    10646..10786
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10972..11560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11647..12441)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12866..12890
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12968..13142
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(13182..14207)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(join(14275..14945,1..7))
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
       61 agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa tacgcaaacc gcctctcccc
      121 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      181 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      241 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      301 aacagctatg acatgattac gccaagctta caaattcggg tcaaggcgga agccagcgcg
      361 ccaccccacg tcagcaaata cggaggcgcg gggttgacgg cgtcacccgg tcctaacggc
      421 gaccaacaaa ccagccagaa gaaattacag taaaaaaaaa gtaaattgca ctttgatcca
      481 ccttttatta cctaagtctc aatttggatc acccttaaac ctatcttttc aatttgggcc
      541 gggttgtggt ttggactacc atgaacaact tttcgtcatg tctaacttcc ctttcagcaa
      601 acatatgaac catatataga ggagatcggc cgtatactag agctgatgtg tttaaggtcg
      661 ttgattgcac gagaaaaaaa aatccaaatc gcaacaatag caaatttatc tggttcaaag
      721 tgaaaagata tgtttaaagg tagtccaaag taaaacttat agataataaa atgtggtcca
      781 aagcgtaatt cactcaaaaa aaatcaacga gacgtgtacc aaacggagac aaacggcatc
      841 ttctcgaaat ttcccaaccg ctcgctcgcc cgcctcgtct tcccggaaac cgcggtggtt
      901 tcagcgtggc ggattctcca agcagacgga gacgtcacgg cacgggactc ctcccaccac
      961 ccaaccgcca taaataccag ccccctcatc tcctctcctc gcatcagctc cacccccgaa
     1021 aaatttctcc ccaatctcgc gaggctctcg tcgtcgaatc gaatcctctc gcgtcctcaa
     1081 ggtacgctgc ttctcctctc ctcgcttcgt ttcgattcga tttcggacgg gtgaggttgt
     1141 tttgttgcta gatccgattg gtggttaggg ttgtcgatgt gattatcgtg agatgtttag
     1201 gggttgtaga tctgatggtt gtgatttggg cacggttggt tcgataggtg gaatcgtggt
     1261 taggttttgg gattggatgt tggttctgat gattgggggg aatttttacg gttagatgaa
     1321 ttgttggatg attcgattgg ggaaatcggt gtagatctgt tggggaattg tggaactagt
     1381 catgcctgag tgattggtgc gatttgtagc gtgttccatc ttgtaggcct tgttgcgagc
     1441 atgttcagat ctactgttcc gctcttgatt gagttattgg tgccatgggt tggtgcaaac
     1501 acaggcttta atatgttata tctgttttgt gtttgatgta gatctgtagg gtagttcttc
     1561 ttagacatgg ttcaattatg tagcttgtgc gtttcgattt gatttcatat gttcacagat
     1621 tagataatga tgaactcttt taattaattg tcaatggtaa ataggaagtc ttgtcgctat
     1681 atctgtcata atgatctcat gttactatct gccagtaatt tatgctaaga actatattag
     1741 aatatcatgt tacaatctgt agtaatatca tgttacaatc tgtagttcat ctatataatc
     1801 tattgtggta atttcttttt actatctgtg tgaagattat tgccactagt tcattctact
     1861 tatttctgaa gttcaggata cgtgtgctgt tactacctat ctgaatacat gtgtgatgtg
     1921 cctgttacta tctttttgaa tacatgtatg ttctgttgga atatggcagg agaccgaggt
     1981 ctcggtttgc tgtttgatcc gttgttgtgt ccttaatctt gtgctagttc ttaccctatc
     2041 tgtttggtga ttatttcttg cagattctag agttatcaac aagtttgtac aaaaaagcag
     2101 gcttcgaagg agatagaacc aattctctaa ggaaatactt aaccatgggt accgccacca
     2161 tgagcaagct ggagaagttt acaaactgct actccctgtc taagaccctg aggttcaagg
     2221 ccatccctgt gggcaagacc caggagaaca tcgacaataa gcggctgctg gtggaggacg
     2281 agaagagagc cgaggattat aagggcgtga agaagctgct ggatcgctac tatctgtctt
     2341 ttatcaacga cgtgctgcac agcatcaagc tgaagaatct gaacaattac atcagcctgt
     2401 tccggaagaa aaccagaacc gagaaggaga ataaggagct ggagaacctg gagatcaatc
     2461 tgcggaagga gatcgccaag gccttcaagg gcaacgaggg ctacaagtcc ctgtttaaga
     2521 aggatatcat cgagacaatc ctgccagagt tcctggacga taaggacgag atcgccctgg
     2581 tgaacagctt caatggcttt accacagcct tcaccggctt ctttgataac agagagaata
     2641 tgttttccga ggaggccaag agcacatcca tcgccttcag gtgtatcaac gagaatctga
     2701 cccgctacat ctctaatatg gacatcttcg agaaggtgga cgccatcttt gataagcacg
     2761 aggtgcagga gatcaaggag aagatcctga acagcgacta tgatgtggag gatttctttg
     2821 agggcgagtt ctttaacttt gtgctgacac aggagggcat cgacgtgtat aacgccatca
     2881 tcggcggctt cgtgaccgag agcggcgaga agatcaaggg cctgaacgag tacatcaacc
     2941 tgtataatca gaaaaccaag cagaagctgc ctaagtttaa gccactgtat aagcaggtgc
     3001 tgagcgatcg ggagtctctg agcttctacg gcgagggcta tacatccgat gaggaggtgc
     3061 tggaggtgtt tagaaacacc ctgaacaaga acagcgagat cttcagctcc atcaagaagc
     3121 tggagaagct gttcaagaat tttgacgagt actctagcgc cggcatcttt gtgaagaacg
     3181 gccccgccat cagcacaatc tccaaggata tcttcggcga gtggaacgtg atccgggaca
     3241 agtggaatgc cgagtatgac gatatccacc tgaagaagaa ggccgtggtg accgagaagt
     3301 acgaggacga tcggagaaag tccttcaaga agatcggctc cttttctctg gagcagctgc
     3361 aggagtacgc cgacgccgat ctgtctgtgg tggagaagct gaaggagatc atcatccaga
     3421 aggtggatga gatctacaag gtgtatggct cctctgagaa gctgttcgac gccgattttg
     3481 tgctggagaa gagcctgaag aagaacgacg ccgtggtggc catcatgaag gacctgctgg
     3541 attctgtgaa gagcttcgag aattacatca aggccttctt tggcgagggc aaggagacaa
     3601 acagggacga gtccttctat ggcgattttg tgctggccta cgacatcctg ctgaaggtgg
     3661 accacatcta cgatgccatc cgcaattatg tgacccagaa gccctactct aaggataagt
     3721 tcaagctgta ttttcagaac cctcagttca tgggcggctg ggacaaggat aaggagacag
     3781 actatcgggc caccatcctg agatacggct ccaagtacta tctggccatc atggataaga
     3841 agtacgccaa gtgcctgcag aagatcgaca aggacgatgt gaacggcaat tacgagaaga
     3901 tcaactataa gctgctgccc ggccctaata agatgctgcc aaaggtgttc ttttctaaga
     3961 agtggatggc ctactataac cccagcgagg acatccagaa gatctacaag aatggcacat
     4021 tcaagaaggg cgatatgttt aacctgaatg actgtcacaa gctgatcgac ttctttaagg
     4081 atagcatctc ccggtatcca aagtggtcca atgcctacga tttcaacttt tctgagacag
     4141 agaagtataa ggacatcgcc ggcttttaca gagaggtgga ggagcagggc tataaggtga
     4201 gcttcgagtc tgccagcaag aaggaggtgg ataagctggt ggaggagggc aagctgtata
     4261 tgttccagat ctataacaag gacttttccg ataagtctca cggcacaccc aatctgcaca
     4321 ccatgtactt caagctgctg tttgacgaga acaatcacgg acagatcagg ctgagcggag
     4381 gagcagagct gttcatgagg cgcgcctccc tgaagaagga ggagctggtg gtgcacccag
     4441 ccaactcccc tatcgccaac aagaatccag ataatcccaa gaaaaccaca accctgtcct
     4501 acgacgtgta taaggataag aggttttctg aggaccagta cgagctgcac atcccaatcg
     4561 ccatcaataa gtgccccaag aacatcttca agatcaatac agaggtgcgc gtgctgctga
     4621 agcacgacga taacccctat gtgatcggca tcgatagggg cgagcgcaat ctgctgtata
     4681 tcgtggtggt ggacggcaag ggcaacatcg tggagcagta ttccctgaac gagatcatca
     4741 acaacttcaa cggcatcagg atcaagacag attaccactc tctgctggac aagaaggaga
     4801 aggagaggtt cgaggcccgc cagaactgga cctccatcga gaatatcaag gagctgaagg
     4861 ccggctatat ctctcaggtg gtgcacaaga tctgcgagct ggtggagaag tacgatgccg
     4921 tgatcgccct ggaggacctg aactctggct ttaagaatag ccgcgtgaag gtggagaagc
     4981 aggtgtatca gaagttcgag aagatgctga tcgataagct gaactacatg gtggacaaga
     5041 agtctaatcc ttgtgcaaca ggcggcgccc tgaagggcta tcagatcacc aataagttcg
     5101 agagctttaa gtccatgtct acccagaacg gcttcatctt ttacatccct gcctggctga
     5161 catccaagat cgatccatct accggctttg tgaacctgct gaaaaccaag tataccagca
     5221 tcgccgattc caagaagttc atcagctcct ttgacaggat catgtacgtg cccgaggagg
     5281 atctgttcga gtttgccctg gactataaga acttctctcg cacagacgcc gattacatca
     5341 agaagtggaa gctgtactcc tacggcaacc ggatcagaat cttccggaat cctaagaaga
     5401 acaacgtgtt cgactgggag gaggtgtgcc tgaccagcgc ctataaggag ctgttcaaca
     5461 agtacggcat caattatcag cagggcgata tcagagccct gctgtgcgag cagtccgaca
     5521 aggccttcta ctctagcttt atggccctga tgagcctgat gctgcagatg cggaacagca
     5581 tcacaggccg caccgacgtg gattttctga tcagccctgt gaagaactcc gacggcatct
     5641 tctacgatag ccggaactat gaggcccagg agaatgccat cctgccaaag aacgccgacg
     5701 ccaatggcgc ctataacatc gccagaaagg tgctgtgggc catcggccag ttcaagaagg
     5761 ccgaggacga gaagctggat aaggtgaaga tcgccatctc taacaaggag tggctggagt
     5821 acgcccagac cagcgtgaag cacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa
     5881 aaaagaaaaa gggatcctac ccatacgatg ttccagatta cgcttatccc tacgacgtgc
     5941 ctgattatgc atacccatat gatgtccccg actatgccta agaattcgcg gccgcactcg
     6001 agatatctag acccagcttt cttgtacaaa gtggttgata acagcgacta caaggatgac
     6061 gatgacaagg cttagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     6121 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     6181 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     6241 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     6301 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cactggccgt
     6361 cgttttacac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6421 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6481 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc
     6541 ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
     6601 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt
     6661 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     6721 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     6781 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     6841 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     6901 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     6961 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     7021 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     7081 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     7141 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     7201 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     7261 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     7321 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
     7381 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     7441 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     7501 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     7561 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     7621 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     7681 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     7741 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     7801 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     7861 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     7921 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     7981 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     8041 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     8101 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     8161 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     8221 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     8281 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     8341 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     8401 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     8461 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     8521 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     8581 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     8641 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     8701 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     8761 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     8821 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     8881 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
     8941 actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca
     9001 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     9061 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     9121 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     9181 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     9241 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     9301 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     9361 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     9421 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     9481 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     9541 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     9601 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     9661 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     9721 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     9781 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     9841 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     9901 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     9961 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
    10021 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
    10081 ggaaaaaggt cgaaaagcac tctttcctgt ggatagcacg tacattggga acccaaagcc
    10141 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
    10201 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
    10261 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
    10321 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
    10381 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
    10441 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
    10501 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10561 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10621 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
    10681 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10741 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
    10801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10921 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11221 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    11281 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    11341 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    11401 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    11461 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11521 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11581 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc
    11641 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    11701 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    11761 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    11821 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    11881 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    11941 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    12001 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    12061 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    12121 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    12181 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    12241 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    12301 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    12361 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    12421 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    12481 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    12541 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    12601 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
    12661 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
    12721 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
    12781 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
    12841 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
    12901 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
    12961 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
    13021 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
    13081 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
    13141 aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt tgccctcgga
    13201 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
    13261 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
    13321 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
    13381 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca
    13441 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
    13501 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
    13561 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
    13621 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
    13681 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
    13741 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
    13801 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc
    13861 gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
    13921 tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact
    13981 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
    14041 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
    14101 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
    14161 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc
    14221 ccgggatctg cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg
    14281 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg
    14341 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
    14401 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
    14461 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    14521 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
    14581 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
    14641 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtta
    14701 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
    14761 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
    14821 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
    14881 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
    14941 tgaaa
//
