LOCUS Exported 10246 bp ds-DNA circular SYN 22-MAR-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 10246) AUTHORS Trial User TITLE Direct Submission JOURNAL Exported Monday, Jul 13, 2020 from SnapGene Viewer 5.0.8 https://www.snapgene.com FEATURES Location/Qualifiers source 1..10246 /organism="recombinant plasmid" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 2448..2628 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2672..2797 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3290..3523 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 4018..4134 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 4168..4188 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 4188..5399 /label=UbC promoter /note="human ubiquitin C promoter" protein_bind complement(5403..5423) /gene="mutant version of attB" /label=attB5 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" LTR 5427..5695 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" CDS 5705..5746 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind 5744..5787 /label=attB Insert.FOR protein_bind 5744..5768 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 5774..7075 /codon_start=1 /label=hGSK3b isoform 2 STOP /translation="MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPG QGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKL DHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMY QLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYY RAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQI REMNPNYTEFKFPQIKAHPWTKDSSGTGHFTSGVRVFRPRTPPEAIALCSRLLEYTPTA RLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQA AASTPTNATAASDANTGDRGQTNNAASASASNST" misc_feature 5774..6197 /label=Sequenced primer_bind 5774..5789 /label=attb1-GSK3-F CDS 6591..6602 /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" misc_feature 6615..7075 /label=Sequenced primer_bind complement(7058..7098) /label=attB Insert.REV primer_bind complement(7058..7075) /label=attB2-GSK3-R protein_bind complement(7074..7098) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 7086..7091 /label=BsrGI-dual Cutsite /note="For cutting in the insert flanking attB, L, P sites (and the cddB gene)" misc_feature 7128..7716 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7181..7201) /label=wPRE-R SeqRevPrimer CDS complement(7599..7610) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 7730..8229 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 8250..8849 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" LTR 8987..9220 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 9292..9413 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 9432..9567 /label=SV40 ori /note="SV40 origin of replication" promoter complement(9600..9618) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9628..9644) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9637..9659) /label=SeqFwd250s Primer rep_origin 9789..10244 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt 4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 4081 aacagacata caaactaaag aattacaaaa caaattacaa aaattcaaaa ttttatcgtc 4141 cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg 4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga 4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc 4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg 4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct 4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga 4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt 4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc 4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt 4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg 4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca 4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga 4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg 4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg 4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg 5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc 5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg 5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct 5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt 5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc 5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg 5401 gccaactttt gtatacaaag tggcgagggg ctcgcatctc tccttcacgc gcccgccgcc 5461 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg 5521 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt 5581 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc 5641 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatcggcac 5701 catgggtaag cctatcccta accctctcct cggtctcgat tctacaactt tgtacaaaaa 5761 agttggcgcc accatgtcag ggcggcccag aaccacctcc tttgcggaga gctgcaagcc 5821 ggtgcagcag ccttcagctt ttggcagcat gaaagttagc agagacaagg acggcagcaa 5881 ggtgacaaca gtggtggcaa ctcctgggca gggtccagac aggccacaag aagtcagcta 5941 tacagacact aaagtgattg gaaatggatc atttggtgtg gtatatcaag ccaaactttg 6001 tgattcagga gaactggtcg ccatcaagaa agtattgcag gacaagagat ttaagaatcg 6061 agagctccag atcatgagaa agctagatca ctgtaacata gtccgattgc gttatttctt 6121 ctactccagt ggtgagaaga aagatgaggt ctatcttaat ctggtgctgg actatgttcc 6181 ggaaacagta tacagagttg ccagacacta tagtcgagcc aaacagacgc tccctgtgat 6241 ttatgtcaag ttgtatatgt atcagctgtt ccgaagttta gcctatatcc attcctttgg 6301 aatctgccat cgggatatta aaccgcagaa cctcttgttg gatcctgata ctgctgtatt 6361 aaaactctgt gactttggaa gtgcaaagca gctggtccga ggagaaccca atgtttcgta 6421 tatctgttct cggtactata gggcaccaga gttgatcttt ggagccactg attatacctc 6481 tagtatagat gtatggtctg ctggctgtgt gttggctgag ctgttactag gacaaccaat 6541 atttccaggg gatagtggtg tggatcagtt ggtagaaata atcaaggtcc tgggaactcc 6601 aacaagggag caaatcagag aaatgaaccc aaactacaca gaatttaaat tccctcaaat 6661 taaggcacat ccttggacta aggattcgtc aggaacagga catttcacct caggagtgcg 6721 ggtcttccga ccccgaactc caccggaggc aattgcactg tgtagccgtc tgctggagta 6781 tacaccaact gcccgactaa caccactgga agcttgtgca cattcatttt ttgatgaatt 6841 acgggaccca aatgtcaaac taccaaatgg gcgagacaca cctgcactct tcaacttcac 6901 cactcaagaa ctgtcaagta atccacctct ggctaccatc cttattcctc ctcatgctcg 6961 gattcaagca gctgcttcaa cccccacaaa tgccacagca gcgtcagatg ctaatactgg 7021 agaccgtgga cagaccaata atgctgcttc tgcatcagct tccaactcca cctgaccaac 7081 tttcttgtac aaagttgtga tatccagcac agtggcggcc gctcgacaat caacctctgg 7141 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 7201 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 7261 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 7321 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 7381 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 7441 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 7501 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 7561 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 7621 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 7681 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctaccg ggtaggggag 7741 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc 7801 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct 7861 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc 7921 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt 7981 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc 8041 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg 8101 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg 8161 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg 8221 ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg 8281 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 8341 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 8401 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 8461 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 8521 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 8581 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 8641 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 8701 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 8761 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 8821 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg 8881 aaaggagcgc acgaccccat gcatctcgag ggcccggtac ctttaagacc aatgacttac 8941 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct 9001 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag 9061 atctgagcct gggagctctc tggctgccta gggaacccac tgcttaagcc tcaataaagc 9121 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 9181 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 9241 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt 9301 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 9361 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 9421 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 9481 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 9541 agtagtgagg aggctttttt ggaggcctag gcttttgcgt cgagacgtac ccaattcgcc 9601 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga 9661 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 9721 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 9781 atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 9841 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 9901 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 9961 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 10021 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 10081 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 10141 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 10201 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcc //