LOCUS Exported 10616 bp ds-DNA circular SYN 10-MAR-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 10616) AUTHORS Dhaval Bhatt TITLE Direct Submission JOURNAL Exported Tuesday, Jul 14, 2020 from SnapGene Viewer 5.0.8 https://www.snapgene.com FEATURES Location/Qualifiers source 1..10616 /organism="recombinant plasmid" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 2448..2628 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2672..2797 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3290..3523 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 4018..4134 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 4168..4188 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 4188..5399 /label=UbC promoter /note="human ubiquitin C promoter" protein_bind complement(5403..5423) /gene="mutant version of attB" /label=attB5 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" misc_feature 5424..5433 /label=Kozak seq CDS 5430..5432 /codon_start=1 /label=Start site /translation="M" CDS 5436..6206 /codon_start=1 /label=miniTurbo /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK" CDS 6207..6212 /codon_start=1 /label=Gap Seq /translation="AS" CDS 6213..6254 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind 6255..6300 /label=attB Insert.FOR protein_bind 6255..6279 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 6262 /label=BsrGI-dual Cutsite /note="For cutting in the insert flanking attB, L, P sites (and the cddB gene)" CDS 6285..7442 /codon_start=1 /label=DK009. CAMK1D (CCDS7091.1) /translation="MARENGESSSSWKKQAEDIKKIFEFKETLGTGAFSEVVLAEEKAT GKLFAVKCIPKKALKGKESSIENEIAVLRKIKHENIVALEDIYESPNHLYLVMQLVSGG ELFDRIVEKGFYTEKDASTLIRQVLDAVYYLHRMGIVHRDLKPENLLYYSQDEESKIMI SDFGLSKMEGKGDVMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFY DENDSKLFEQILKAEYEFDSPYWDDISDSAKDFIRNLMEKDPNKRYTCEQAARHPWIAG DTALNKNIHESVSAQIRKNFAKSKWRQAFNATAVVRHMRKLHLGSSLDSSNASVSSSLS LASQKDCLAPSTLCSFISSSSGVSGVGAERRPRPTTVTAVHSGSK" misc_feature 7230..7232 /label=Silent V316>V mutation /note="C948>G mutation" primer_bind complement(7421..7465) /label=attB Insert.REV protein_bind complement(7444..7468) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 7456..7461 /label=BsrGI-dual Cutsite /note="For cutting in the insert flanking attB, L, P sites (and the cddB gene)" misc_feature 7498..8086 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7551..7571) /label=wPRE-R SeqRevPrimer CDS complement(7969..7980) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 8100..8599 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" CDS 8620..9219 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" LTR 9357..9590 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 9662..9783 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 9802..9937 /label=SV40 ori /note="SV40 origin of replication" promoter complement(9970..9988) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9998..10014) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10007..10029) /label=SeqFwd250s Primer rep_origin 10159..10614 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt 4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 4081 aacagacata caaactaaag aattacaaaa caaattacaa aaattcaaaa ttttatcgtc 4141 cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg 4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga 4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc 4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg 4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct 4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga 4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt 4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc 4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt 4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg 4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca 4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga 4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg 4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg 4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg 5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc 5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg 5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct 5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt 5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc 5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg 5401 gccaactttt gtatacaaag tgggccacca tgggaatccc gctgctgaac gctaaacaga 5461 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc 5521 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc 5581 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt 5641 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca 5701 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat 5761 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg 5821 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc 5881 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc 5941 tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt 6001 tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca 6061 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg 6121 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag 6181 aaatcagtct gcggtctgcc gaaaaggcta gcggcaagcc catccccaac cccctgctgg 6241 gcctggacag caccacaact ttgtacaaaa aagcaggctc caccatggcc cgggagaacg 6301 gcgagagcag ctcctcctgg aaaaagcaag ctgaagacat caagaagatc ttcgagttca 6361 aagagaccct cggaaccggg gccttttccg aagtggtttt agctgaagag aaggcaactg 6421 gcaagctctt tgctgtgaag tgtatcccta agaaggcgct gaagggcaag gaaagcagca 6481 tagagaatga gatagccgtc ctgagaaaga ttaagcatga aaatattgtt gccctggaag 6541 acatttatga aagcccaaat cacctgtact tggtcatgca gctggtgtcc ggtggagagc 6601 tgtttgaccg gatagtggag aaggggtttt atacagagaa ggatgccagc actctgatcc 6661 gccaagtctt ggacgccgtg tactatctcc acagaatggg catcgtccac agagacctca 6721 agcccgaaaa tctcttgtac tacagtcaag atgaggagtc caaaataatg atcagtgact 6781 ttggattgtc aaaaatggag ggcaaaggag atgtgatgtc cactgcctgt ggaactccag 6841 gctatgtcgc tcctgaagtc ctcgcccaga aaccttacag caaagccgtt gactgctggt 6901 ccatcggagt gattgcctac atcttgctct gcggctaccc tcctttttat gatgaaaatg 6961 actccaagct ctttgagcag atcctcaagg cggaatatga gtttgactct ccctactggg 7021 atgacatctc cgactctgca aaagacttca ttcggaacct gatggagaag gacccgaata 7081 aaagatacac gtgtgagcag gcagctcggc acccatggat cgctggtgac acagccctca 7141 acaaaaacat ccacgagtcc gtcagcgccc agatccggaa aaactttgcc aagagcaaat 7201 ggagacaagc atttaatgcc acggccgtcg tcagacatat gagaaaacta cacctcggca 7261 gcagcctgga cagttcaaat gcaagtgttt cgagcagcct cagtttggcc agccaaaaag 7321 actgtctggc accttccacg ctctgtagtt tcatttcttc ttcgtcgggg gtctcaggag 7381 ttggagccga gcggagaccc aggcccacca ctgtgacggc agtgcactct ggaagcaagt 7441 aggacccagc tttcttgtac aaagttgtga tatccagcac agtggcggcc gctcgacaat 7501 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 7561 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 7621 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 7681 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 7741 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 7801 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 7861 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 7921 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 7981 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 8041 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctaccg 8101 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 8161 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 8221 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 8281 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 8341 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 8401 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 8461 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 8521 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 8581 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 8641 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 8701 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 8761 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 8821 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 8881 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 8941 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 9001 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 9061 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 9121 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 9181 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag 9241 cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac ctttaagacc 9301 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 9361 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg 9421 gttagaccag atctgagcct gggagctctc tggctgccta gggaacccac tgcttaagcc 9481 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 9541 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 9601 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 9661 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 9721 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 9781 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg 9841 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 9901 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcgt cgagacgtac 9961 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc 10021 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg 10081 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 10141 tgaatggcga atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 10201 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 10261 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 10321 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 10381 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 10441 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 10501 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 10561 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcc //