LOCUS       Exported               10940 bp ds-DNA     circular SYN 10-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   dhaval.bhatt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 14, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4018..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     protein_bind    complement(5403..5423)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5424..5433
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5436..6206
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6213..6254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6255..6279
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6263..6267
                     /label=BsrGI-dual Cutsite
                     /note="For cutting in the insert flanking attB, L, P sites 
                     (and the cddB gene)"
     misc_feature    6280..6281
                     /label=NN Fill for N-term tagging
     misc_feature    6282..6284
                     /label=HvdLib Fill
     CDS             6285..7766
                     /codon_start=1
                     /product="iso 1"
                     /label=DK030. CDLK2 (CCDS3570.1)
                     /translation="MEKYENLGLVGEGSYGMVMKCRNKDTGRIVAIKKFLESDDDKMVK
                     KIAMREIKLLKQLRHENLVNLLEVCKKKKRWYLVFEFVDHTILDDLELFPNGLDYQVVQ
                     KYLFQIINGIGFCHSHNIIHRDIKPENILVSQSGVVKLCDFGFARTLAAPGEVYTDYVA
                     TRWYRAPELLVGDVKYGKAVDVWAIGCLVTEMFMGEPLFPGDSDIDQLYHIMMCLGNLI
                     PRHQELFNKNPVFAGVRLPEIKEREPLERRYPKLSEVVIDLAKKCLHIDPDKRPFCAEL
                     LHHDFFQMDGFAERFSQELQLKVQKDARNVSLSKKSQNRKKEKEKDDSLVEERKTLVVQ
                     DTNADPKIKDYKLFKIKGSKIDGEKAEKGNRASNASCLHDSRTSHNKIVPSTSLKDCSN
                     VSVDHTRNPSVAIPPLTHNLSAVAPSINSGMGTETIPIQGYRVDEKTKKCSIPFVKPNR
                     HSPSGIYNINVTTLVSGPPLSDDSGADLPQMEHQH"
     CDS             7419..7421
                     /codon_start=1
                     /product="A>1138G (H379>R) mutation
                     
                     "
                     /label=A>1138G (H379>R) mutation
                     /translation="H"
     CDS             7455..7457
                     /codon_start=1
                     /product="T928>C (S391>P) mutation
                     
                     "
                     /label=T928>C (S391>P) mutation
                     /translation="S"
     misc_feature    7767
                     /label=N Fill for C-term tagging
     protein_bind    complement(7768..7792)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7778..7798
                     /label=663-UbC-mTID-R1-R2
                     /note="663-UbC-mTID-R1-R2 "
     misc_feature    7822..8410
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8293..8304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8424..8923
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             8944..9543
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             9681..9914
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9986..10107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      10126..10261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(10294..10312)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10322..10338)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10483..10938
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     5401 gccaactttt gtatacaaag tgggccacca tgggaatccc gctgctgaac gctaaacaga
     5461 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     5521 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     5581 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     5641 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     5701 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     5761 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     5821 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     5881 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     5941 tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt
     6001 tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca
     6061 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     6121 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     6181 aaatcagtct gcggtctgcc gaaaaggcta gcggcaagcc catccccaac cccctgctgg
     6241 gcctggacag caccacaagt ttgtacaaaa aagcaggctc caccatggaa aaatatgaaa
     6301 acctgggttt ggttggagaa gggagttatg gaatggtgat gaagtgtagg aataaagata
     6361 ctggaagaat tgtggccata aagaagttct tagaaagtga cgatgacaaa atggttaaaa
     6421 agattgcaat gcgagaaatc aagttactaa agcaacttag gcatgaaaac ttggtgaatc
     6481 tcttggaagt gtgtaagaaa aaaaaacgat ggtacctagt ctttgaattt gttgaccaca
     6541 caattcttga tgacttggag ctctttccaa atggactaga ctaccaagta gttcaaaagt
     6601 atttgtttca gattattaat ggaattggat tttgtcacag tcacaatatc atacacagag
     6661 atataaagcc agagaatata ttagtctccc agtctggcgt tgtcaagcta tgcgattttg
     6721 gatttgcgcg aacattggca gctcctgggg aggtttatac tgattatgtg gcaacccgat
     6781 ggtacagagc tccagaacta ttggttggtg atgtcaagta tggcaaggct gttgatgtgt
     6841 gggccattgg ttgtctggta actgaaatgt tcatggggga acccctattt cctggagatt
     6901 ctgatattga tcagctatat catattatga tgtgtttagg taatctaatt ccaaggcatc
     6961 aggagctttt taataaaaat cctgtgtttg ctggagtaag gttgcctgaa atcaaggaaa
     7021 gagaacctct tgaaagacgc tatcctaagc tctctgaagt ggtgatagat ttagcaaaga
     7081 aatgcttaca tattgacccc gacaaaagac ccttctgtgc tgagctccta caccatgatt
     7141 tctttcaaat ggatggattt gctgagaggt tttcccaaga actacagtta aaagtacaga
     7201 aagatgccag aaatgtttct ttatctaaaa aatcccaaaa cagaaagaag gaaaaagaaa
     7261 aagatgattc cttagttgaa gaaagaaaaa cacttgtggt acaggatacc aatgctgatc
     7321 ccaaaattaa ggattataaa ctatttaaaa taaaaggctc aaaaattgat ggagaaaaag
     7381 ctgaaaaagg caatagagct tcaaatgcca gctgtctcca tgacagtagg acaagccaca
     7441 acaaaatagt gccttcaaca agcctcaaag actgcagcaa tgtcagcgtg gaccacacaa
     7501 ggaatccaag cgtggcaatt cccccactta cacacaatct ttctgcagtt gctcccagca
     7561 ttaattctgg aatggggact gagactatac caattcaggg ttacagagtg gatgagaaaa
     7621 ctaagaagtg ttctattcca tttgttaaac cgaacagaca ttccccatca ggcatttata
     7681 acattaatgt gaccacatta gtatcaggac ctcccctgtc agatgattca ggggctgatt
     7741 tgcctcaaat ggaacaccag cactaggacc cagctttctt gtacaaagtg gtgatatcca
     7801 gcacagtggc ggccgctcga caatcaacct ctggattaca aaatttgtga aagattgact
     7861 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7921 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7981 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8041 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8101 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8161 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     8221 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8281 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8341 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8401 gcctccccgc ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     8461 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     8521 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     8581 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     8641 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     8701 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     8761 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     8821 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     8881 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
     8941 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     9001 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     9061 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     9121 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     9181 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     9241 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     9301 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     9361 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     9421 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     9481 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     9541 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatct
     9601 cgagggcccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     9661 tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct
     9721 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggctg
     9781 cctagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     9841 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     9901 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     9961 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
    10021 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10081 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
    10141 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    10201 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    10261 ctaggctttt gcgtcgagac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
    10321 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
    10381 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
    10441 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg
    10501 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    10561 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    10621 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    10681 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    10741 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10801 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    10861 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
    10921 tattaacgtt tacaatttcc
//
