LOCUS       Exported               11357 bp ds-DNA     circular SYN 10-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   dhaval.bhatt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 14, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4018..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     protein_bind    complement(5403..5423)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5424..5433
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5436..6206
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6213..6254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6255..6279
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6263..6267
                     /label=BsrGI-dual Cutsite
                     /note="For cutting in the insert flanking attB, L, P sites 
                     (and the cddB gene)"
     misc_feature    6280..6281
                     /label=NN Fill for N-term tagging
     misc_feature    6282..6284
                     /label=HvdLib Fill
     CDS             6285..8183
                     /codon_start=1
                     /product="iso 1 (CCDS4949.1)"
                     /label=DK055. ICK (CCDS4949.1)
                     /translation="MNRYTTIRQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEEC
                     MNLREVKSLKKLNHANVVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAIRN
                     IMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSKPPYTDYVSTR
                     WYRAPEVLLRSTNYSSPIDVWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGTPKKT
                     DWPEGYQLSSAMNFRWPQCVPNNLKTLIPNASSEAVQLLRDMLQWDPKKRPTASQALRY
                     PYFQVGHPLGSTTQNLQDSEKPQKGILEKAGPPPYIKPVPPAQPPAKPHTRISSRQHQA
                     SQPPLHLTYPYKAEVSRTDHPSHLQEDKPSPLLFPSLHNKHPQSKITAGLEHKNGEIKP
                     KSRRRWGLISRSTKDSDDWADLDDLDFSPSLSRIDLKNKKRQSDDTLCRFESVLDLKPS
                     EPVGTGNSAPTQTSYQRRDTPTLRSAAKQHYLKHSRYLPGISIRNGILSNPGKEFIPPN
                     PWSSSGLSGKSSGTMSVISKVNSVGSSSTSSSGLTGNYVPSFLKKEIGSAMQRVHLAPI
                     PDPSPGYSSLKAMRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRTDWASKYASRR"
     RBS             7223..7231
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7926..7928
                     /codon_start=1
                     /product="C1643>T(S548>F) mutation (Change in amino acid 
                     from S to F)"
                     /label=C1643>T(S548>F)
                     /translation="S"
     misc_feature    8184
                     /label=N Fill for C-term tagging
     protein_bind    complement(8185..8209)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8239..8827
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8710..8721)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8841..9340
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             9361..9960
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             10098..10331
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10403..10524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      10543..10678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(10711..10729)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10739..10755)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10900..11355
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcacaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     5401 gccaactttt gtatacaaag tgggccacca tgggaatccc gctgctgaac gctaaacaga
     5461 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     5521 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     5581 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     5641 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     5701 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     5761 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     5821 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     5881 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     5941 tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt
     6001 tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca
     6061 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     6121 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     6181 aaatcagtct gcggtctgcc gaaaaggcta gcggcaagcc catccccaac cccctgctgg
     6241 gcctggacag caccacaagt ttgtacaaaa aagcaggctc caccatgaat agatacacaa
     6301 caatcaggca gctcggggat ggaacctacg gttccgtcct gctgggaaga agcattgagt
     6361 ctggggagct gatcgctatt aaaaaaatga aaagaaaatt ttattcctgg gaggaatgca
     6421 tgaaccttcg ggaggttaag tctttaaaga agctcaacca tgccaatgta gtcaaattaa
     6481 aagaagttat cagggaaaat gatcatcttt attttatctt cgagtacatg aaggaaaatc
     6541 tttaccagct cattaaagag agaaataagt tgtttcctga gtctgctata aggaatatca
     6601 tgtatcagat attacaagga ctcgcattta ttcacaaaca cggcttcttt catcgagact
     6661 taaagcctga gaacctcctc tgcatgggac cagaacttgt gaaaattgca gactttggtt
     6721 tggcccgaga aatacgatca aaacctccat atacagatta tgtatctacc agatggtaca
     6781 gggctccaga agtactcctg aggtctacca actacagctc ccccattgac gtctgggcgg
     6841 tgggctgcat catggcagaa gtttacaccc tcaggccact cttccctgga gccagtgaaa
     6901 ttgacacaat attcaaaatt tgccaagtgc tggggacacc aaaaaagact gactggcctg
     6961 aaggctatca actttcaagt gcaatgaact tccgttggcc acagtgtgta cccaataact
     7021 taaagacctt gattcccaat gctagcagtg aagcagtcca gctcctgaga gacatgcttc
     7081 agtgggatcc caagaaacga ccaacagcta gtcaggcact tcgatatcct tacttccaag
     7141 ttggacaccc actaggcagc accacacaaa accttcagga ttcagaaaaa ccacagaaag
     7201 gcatcctgga aaaggcaggc ccacctcctt atattaagcc agtcccacct gcccagccac
     7261 cagccaagcc acacacacga atttcttcac gacagcatca agccagccag ccccctctgc
     7321 atctcacgta cccctacaaa gcagaggtct ccaggacaga tcacccaagc catctccagg
     7381 aggacaagcc aagcccgttg cttttcccat ccctccacaa caagcatcca cagtcgaaaa
     7441 tcacagctgg cctggagcac aaaaatggtg agataaagcc aaagagtagg agaaggtggg
     7501 gtcttatttc caggtcaaca aaggattcag atgattgggc tgacttggat gacttggatt
     7561 tcagtccatc cctcagcagg attgacctga aaaacaagaa aagacagagt gatgacactc
     7621 tctgcaggtt tgagagtgtt ttggacctga agccctctga gcctgtgggc acaggaaaca
     7681 gtgcccccac ccagacgtca tatcagcggc gagacacgcc caccctgaga tctgcagcca
     7741 agcagcacta tttgaagcac tctcgatact tgcctgggat cagtataaga aatggcatac
     7801 tctcgaatcc aggcaaggaa tttattccac ctaatccatg gtctagttct ggcttgtctg
     7861 gaaaatcttc agggacaatg tcagtaatca gcaaagtaaa ttcagttggt tccagctcta
     7921 caagttctag tggactgact ggaaactatg tcccttcctt tctgaaaaaa gaaatcggtt
     7981 ctgctatgca gagggtacac ctagcaccta ttccagaccc ttcccctggt tattcctccc
     8041 tgaaggccat gagacctcat cctgggcgac cattcttcca cacccagcct agaagcactc
     8101 ctgggttgat accacggcct ccagccgccc agccagtgca tggccggaca gactgggctt
     8161 ccaagtacgc atctcggcga taggacccag ctttcttgta caaagtggtg atatccagca
     8221 cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8281 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8341 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8401 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8461 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8521 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8581 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     8641 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8701 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8761 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8821 tccccgcctg gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     8881 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     8941 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     9001 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     9061 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     9121 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     9181 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     9241 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     9301 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
     9361 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     9421 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     9481 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     9541 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     9601 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     9661 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     9721 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     9781 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     9841 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     9901 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     9961 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatctcga
    10021 gggcccggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
    10081 aaaagaaaag gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt
    10141 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctgcct
    10201 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10261 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10321 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
    10381 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
    10441 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10501 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
    10561 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    10621 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    10681 ggcttttgcg tcgagacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
    10741 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    10801 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    10861 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
    10921 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    10981 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    11041 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    11101 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    11161 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    11221 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    11281 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    11341 taacgtttac aatttcc
//
