LOCUS       Exported               11143 bp ds-DNA     circular SYN 10-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11143)
  AUTHORS   dhaval.bhatt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 14, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11143
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4018..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4166..4186
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4186..5397
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5398..5445
                     /label=attB Insert.FOR
     protein_bind    5398..5422
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5422..7127
                     /codon_start=1
                     /product="CCDS31000.1 iso 2"
                     /label=DK48. DYRK3 (CCDS31000.1)
                     /translation="MKWKEKLGDGVYDTFMMIDETKCPPCSNVLCNPSEPPPPRRLNMT
                     TEQFTGDHTQHFLDGGEMKVEQLFQEFGNRKSNTIQSDGISDSEKCSPTVSQGKSSDCL
                     NTVKSNSSSKAPKVVPLTPEQALKQYKHHLTAYEKLEIINYPEIYFVGPNAKKRHGVIG
                     GPNNGGYDDADGAYIHVPRDHLAYRYEVLKIIGKGSFGQVARVYDHKLRQYVALKMVRN
                     EKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTFRNHVCMAFELLSIDLYELIKK
                     NKFQGFSVQLVRKFAQSILQSLDALHKNKIIHCDLKPENILLKHHGRSSTKVIDFGSSC
                     FEYQKLYTYIQSRFYRAPEIILGSRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLA
                     CMMELLGMPPPKLLEQSKRAKYFINSKGIPRYCSVTTQADGRVVLVGGRSRRGKKRGPP
                     GSKDWGTALKGCDDYLFIEFLKRCLHWDPSARLTPAQALRHPWISKSVPRPLTTIDKVS
                     GKRVVNPASAFQGLGSKLPPVVGIANKLKANLMSETNGSIPLCSVLPKLIS"
     primer_bind     complement(7100..7150)
                     /label=attB Insert.REV
     protein_bind    complement(7127..7151)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7153..7194
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7201..7971
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(7977..7997)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    8026..8614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8497..8508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8627..9126
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             9147..9746
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             9884..10117
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10189..10310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      10329..10464
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(10497..10515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10525..10541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10686..11141
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcaact ttgtatagaa aagttggcct ccgcgccggg
     4201 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
     4261 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
     4321 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
     4381 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
     4441 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
     4501 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
     4561 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
     4621 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
     4681 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
     4741 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
     4801 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
     4861 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
     4921 actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt
     4981 gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt
     5041 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
     5101 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     5161 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     5221 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     5281 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     5341 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacaca
     5401 agtttgtaca aaaaagttgg catgaagtgg aaagagaagt tgggggatgg tgtctatgac
     5461 accttcatga tgatagatga aaccaaatgt cccccctgtt caaatgtact ctgcaatcct
     5521 tctgaaccac ctccacccag aagactaaat atgaccactg agcagtttac aggagatcat
     5581 actcagcact ttttggatgg aggtgagatg aaggtagaac agctgtttca agaatttggc
     5641 aacagaaaat ccaatactat tcagtcagat ggcatcagtg actctgaaaa atgctctcct
     5701 actgtttctc agggtaaaag ttcagattgc ttgaatacag taaaatccaa cagttcatcc
     5761 aaggcaccca aagtggtgcc tctgactcca gaacaagccc tgaagcaata taaacaccac
     5821 ctcactgcct atgagaaact ggaaataatt aattatccag aaatttactt tgtaggtcca
     5881 aatgccaaga aaagacatgg agttattggt ggtcccaata atggagggta tgatgatgca
     5941 gatggggcct atattcatgt acctcgagac catctagctt atcgatatga ggtgctgaaa
     6001 attattggca aggggagttt tgggcaggtg gccagggtct atgatcacaa acttcgacag
     6061 tacgtggccc taaaaatggt gcgcaatgag aagcgctttc atcgtcaagc agctgaggag
     6121 atccggattt tggagcatct taagaaacag gataaaactg gtagtatgaa cgttatccac
     6181 atgctggaaa gtttcacatt ccggaaccat gtttgcatgg cctttgaatt gctgagcata
     6241 gacctttatg agctgattaa aaaaaataag tttcagggtt ttagcgtcca gttggtacgc
     6301 aagtttgccc agtccatctt gcaatctttg gatgccctcc acaaaaataa gattattcac
     6361 tgcgatctga agccagaaaa cattctcctg aaacaccacg ggcgcagttc aaccaaggtc
     6421 attgactttg ggtccagctg tttcgagtac cagaagctct acacatatat ccagtctcgg
     6481 ttctacagag ctccagaaat catcttagga agccgctaca gcacaccaat tgacatatgg
     6541 agttttggct gcatccttgc agaactttta acaggacagc ctctcttccc tggagaggat
     6601 gaaggagacc agttggcctg catgatggag cttctaggga tgccaccacc aaaacttctg
     6661 gagcaatcca aacgtgccaa gtactttatt aattccaagg gcataccccg ctactgctct
     6721 gtgactaccc aggcagatgg gagggttgtg cttgtggggg gtcgctcacg taggggtaaa
     6781 aagcggggtc ccccaggcag caaagactgg gggacagcac tgaaagggtg tgatgactac
     6841 ttgtttatag agttcttgaa aaggtgtctt cactgggacc cctctgcccg cttgacccca
     6901 gctcaagcat taagacaccc ttggattagc aagtctgtcc ccagacctct caccaccata
     6961 gacaaggtgt cagggaaacg ggtagttaat cctgcaagtg ctttccaggg attgggttct
     7021 aagctgcctc cagttgttgg aatagccaat aagcttaaag ctaacttaat gtcagaaacc
     7081 aatggtagta tacccctatg cagtgtattg ccaaaactga ttagctaccc aactttcttg
     7141 tacaaagtgg gcggcaagcc catccccaac cccctgctgg gcctggacag caccgctagc
     7201 atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc
     7261 ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt
     7321 ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg
     7381 ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca
     7441 gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg
     7501 ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg
     7561 gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc
     7621 gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc
     7681 acactgcagg aagcagggat taacctggac aggaatactc tggccgctat gctgatccga
     7741 gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg
     7801 tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag
     7861 attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga
     7921 gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa gtaagccaac
     7981 tttattatac atagttgata tccagcacag tggcggccgc tcgacaatca acctctggat
     8041 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8101 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8161 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8221 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8281 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8341 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8401 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     8461 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8521 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8581 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcaccgggt aggggaggcg
     8641 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     8701 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     8761 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     8821 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     8881 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     8941 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     9001 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     9061 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     9121 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     9181 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     9241 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     9301 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     9361 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     9421 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     9481 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     9541 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     9601 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     9661 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     9721 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     9781 ggagcgcacg accccatgca tctcgagggc ccggtacctt taagaccaat gacttacaag
     9841 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac
     9901 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     9961 tgagcctggg agctctctgg ctgcctaggg aacccactgc ttaagcctca ataaagcttg
    10021 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    10081 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
    10141 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
    10201 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    10261 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    10321 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    10381 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10441 agtgaggagg cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta
    10501 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
    10561 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    10621 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    10681 gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    10741 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    10801 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    10861 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    10921 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    10981 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    11041 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    11101 atttaacgcg aattttaaca aaatattaac gtttacaatt tcc
//
