LOCUS       Exported               13087 bp ds-DNA     circular SYN 10-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   dhaval.bhatt
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 14, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4018..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4166..4186
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4186..5397
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     protein_bind    5398..5422
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5406..5410
                     /label=BsrGI-dual Cutsite
                     /note="For cutting in the insert flanking attB, L, P sites 
                     (and the cddB gene)"
     CDS             5417..5421
                     /codon_start=1
                     /label=Orfeome seq
                     /translation="L"
     CDS             5425..9072
                     /codon_start=1
                     /label=HIPK3 (CCDS7884.1)
                     /translation="MASQVLVYPPYVYQTQSSAFCSVKKLKVEPSSCVFQERNYPRTYV
                     NGRNFGNSHPPTKGSAFQTKIPFNRPRGHNFSLQTSAVVLKNTAGATKVIAAQAQQAHV
                     QAPQIGAWRNRLHFLEGPQRCGLKRKSEELDNHSSAMQIVDELSILPAMLQTNMGNPVT
                     VVTATTGSKQNCTTGEGDYQLVQHEVLCSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEI
                     VAIKILKNHPSYARQGQIEVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQN
                     LYDFLKQNKFSPLPLKVIRPILQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRV
                     KVIDFGSASHVSKTVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLY
                     PGALEYDQIRYISQTQGLPGEQLLNVGTKSTRFFCKETDMSHSGWRLKTLEEHEAETGM
                     KSKEARKYIFNSLDDVAHVNTVMDLEGSDLLAEKADRREFVSLLKKMLLIDADLRITPA
                     ETLNHPFVNMKHLLDFPHSNHVKSCFHIMDICKSHLNSCDTNNHNKTSLLRPVASSSTA
                     TLTANFTKIGTLRSQALTTSAHSVVHHGIPLQAGTAQFGCGDAFQQTLIICPPAIQGIP
                     ATHGKPTSYSIRVDNTVPLVTQAPAVQPLQIRPGVLSQTWSGRTQQMLVPAWQQVTPLA
                     PATTTLTSESVAGSHRLGDWGKMISCSNHYNSVMPQPLLTNQITLSAPQPVSVGIAHVV
                     WPQPATTKKNKQCQNRGILVKLMEWEPGREEINAFSWSNSLQNTNIPHSAFISPKIING
                     KDVEEVSCIETQDNQNSEGEARNCCETSIRQDSDSSVSDKQRQTIIIADSPSPAVSVIT
                     ISSDTDEEETSQRHSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSPSP
                     CKRPNSMSDEEQESSCDTVDGSPTSDSSGHDSPFAESTFVEDTHENTELVSSADTETKP
                     AVCSVVVPPVELENGLNADEHMANTDSICQPLIKGRSAPGRLNQPSAVGTRQQKLTSAF
                     QQQHLNFSQVQHFGSGHQEWNGNFGHRRQQAYIPTSVTSNPFTLSHGSPNHTAVHAHLA
                     GNTHLGGQPTLLPYPSSATLSSAAPVAHLLASPCTSRPMLQHPTYNISHPSGIVHQVPV
                     GLNPRLLPSPTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQYPYML"
     protein_bind    complement(9071..9095)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9097..9138
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             9145..9915
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(9921..9941)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    9970..10558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10441..10452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10571..11070
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             11091..11690
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             11828..12061
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12133..12254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      12273..12408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(12441..12459)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12469..12485)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      12630..13085
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcaact ttgtatagaa aagttggcct ccgcgccggg
     4201 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
     4261 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
     4321 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
     4381 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
     4441 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
     4501 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
     4561 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
     4621 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
     4681 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
     4741 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
     4801 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
     4861 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
     4921 actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt
     4981 gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt
     5041 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
     5101 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     5161 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     5221 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     5281 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     5341 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacaca
     5401 agtttgtaca aaaaagttgg caccatggcc tcacaagtct tggtctaccc accatatgtt
     5461 tatcaaactc agtcaagtgc cttttgtagt gtgaagaaac tcaaagtaga gccaagcagt
     5521 tgtgtattcc aggaaagaaa ctatccacgg acctatgtga atggtagaaa ctttggaaat
     5581 tctcatcctc ccactaaggg tagtgctttt cagacaaaga taccatttaa tagacctcga
     5641 ggacacaact tttcattgca gacaagtgct gttgttttga aaaacactgc aggtgctaca
     5701 aaggtcatag cagctcaggc acagcaagct cacgtgcagg cacctcagat tggggcgtgg
     5761 cgaaacagat tgcatttcct agaaggcccc cagcgatgtg gattgaagcg caagagtgag
     5821 gagttggata atcatagcag cgcaatgcag attgtcgatg aattgtccat acttcctgca
     5881 atgttgcaaa ccaacatggg aaatccagtg acagttgtga cagctaccac aggatcaaaa
     5941 cagaattgta ccactggaga aggtgactat cagttagtac agcatgaagt cttatgctcc
     6001 atgaaaaata cttacgaagt ccttgatttt cttggtcgag gcacgtttgg ccaggtagtt
     6061 aaatgctgga aaagagggac aaatgaaatt gtagcaatca aaattttgaa gaatcatcct
     6121 tcttatgccc gtcaaggtca aatagaagtg agcatattag caaggctcag tactgaaaat
     6181 gctgatgaat ataactttgt acgagcttat gaatgctttc agcaccgtaa ccatacttgt
     6241 ttagtctttg agatgctgga acaaaacttg tatgactttc tgaaacaaaa taaatttagt
     6301 cccctgccac taaaagtgat tcggcccatt cttcaacaag tggccactgc actgaaaaaa
     6361 ttgaaaagtc ttggtttaat tcatgctgat ctcaagccag agaatattat gttggtggat
     6421 cctgttcggc agccttacag ggttaaagta atagactttg ggtcggccag tcatgtatca
     6481 aagactgttt gttcaacata tctacaatct cggtactaca gagctccaga gattatattg
     6541 gggttgccat tttgtgaagc catagacatg tggtcattgg gatgtgtgat tgcagaatta
     6601 tttcttggat ggccgctcta cccaggagcc ttggagtatg atcagattcg atacatttct
     6661 cagactcaag gtttgccagg agaacagttg ttaaatgtgg gtactaaatc cacaagattt
     6721 ttttgcaaag aaacagatat gtctcattct ggttggagat taaagacatt ggaagagcat
     6781 gaggcagaga caggaatgaa gtctaaagaa gccagaaaat acattttcaa cagtctggat
     6841 gatgtagcgc atgtgaacac agtgatggat ttggaaggaa gtgatctttt ggctgagaaa
     6901 gctgatagaa gagaatttgt tagtctgttg aagaaaatgt tgctgattga tgcagattta
     6961 agaattactc cagctgagac cctgaaccat ccttttgtta atatgaaaca tcttctagat
     7021 ttccctcata gcaaccatgt aaagtcctgt tttcatatta tggatatttg taagtcccac
     7081 ctaaattcat gtgacacaaa taatcacaac aaaacttcac ttttaagacc agttgcttca
     7141 agcagtactg ctacactgac tgcaaatttt actaaaatcg gaacattaag aagtcaggca
     7201 ttgaccacat ctgctcattc agttgtgcac catggaatac ctctgcaggc aggaactgct
     7261 cagtttggtt gtggtgatgc ttttcagcag acattgatta tctgtccccc agctattcaa
     7321 ggtattcctg caacacatgg taaacccacc agttattcaa taagggtaga taatacagtt
     7381 ccacttgtaa ctcaggcccc agctgtgcag ccactacaga tccgaccagg agttctttct
     7441 cagacgtggt ctggtagaac acagcagatg ctggtgcctg cctggcaaca ggtgacaccc
     7501 ctggctcctg ctactactac actaacttct gagagtgtgg ctggttcaca caggcttgga
     7561 gactggggga agatgatttc atgcagcaat cattataact cagtgatgcc gcagcctctt
     7621 ctgaccaatc agataacttt atctgcccct cagccagtta gtgtggggat tgcacatgtt
     7681 gtctggcctc agcctgccac taccaagaaa aataaacagt gccagaacag aggtattttg
     7741 gtaaaactaa tggaatggga gccaggaaga gaggaaataa atgctttcag ttggagtaat
     7801 tcattacaga ataccaatat cccacattca gcatttattt ctccaaagat aattaatggg
     7861 aaagatgtcg aggaagtaag ttgtatagaa acacaggaca atcagaactc agaaggagag
     7921 gcaagaaatt gctgtgaaac atctatcaga caggactctg attcatcagt ttcagacaaa
     7981 cagcggcaaa ccatcattat tgccgactcc ccgagtcctg cagtgagtgt catcactatc
     8041 agcagtgaca ctgatgagga agagacttcc cagagacatt cactcagaga atgtaaaggt
     8101 agtctagatt gtgaagcttg ccagagcact ttgaatattg atcggatgtg ttcattaagt
     8161 agtcctgata gtactctgag taccagctcc tcagggcagt ccagcccatc cccctgcaag
     8221 agaccgaata gtatgtcaga tgaagagcaa gaaagtagtt gtgatacggt ggatggctct
     8281 ccgacatctg actcttccgg gcatgacagt ccatttgcag agagcacttt tgtggaggac
     8341 actcatgaaa acacagaatt ggtatcctct gctgacacag aaaccaagcc agctgtctgt
     8401 tctgttgtgg tgccaccagt ggaactagaa aatggcttaa atgccgatga gcatatggca
     8461 aacacagatt ctatatgcca gccattaata aaaggacgat ctgcccctgg aagattaaac
     8521 cagccttctg cagtgggtac tcgtcagcaa aaattgacat cagcattcca gcagcagcat
     8581 ttgaacttca gtcaggttca gcactttgga tctgggcatc aagagtggaa tggaaacttt
     8641 gggcacagaa gacagcaagc ttatattcct actagtgtta ccagtaatcc attcactctt
     8701 tctcatggaa gtcccaatca cacagcagtg catgcccacc tggctggaaa tacacacctc
     8761 ggaggacagc ctactctact tccataccca tcatcagcca ccctcagtag tgctgcacca
     8821 gtggcccacc tgttagcctc tccgtgtacc tcaagaccta tgttacagca tccaacttat
     8881 aatatctccc atcccagtgg catagttcac caagtcccag tgggcttaaa tccccgtctg
     8941 ttaccatccc caaccattca tcagactcag tacaaaccaa tcttcccacc acattcttac
     9001 attgcagcat cacctgcata tactggattt ccactgagtc caacaaaact cagccagtat
     9061 ccatatatgt tgccaacttt cttgtacaaa gtgggcggca agcccatccc caaccccctg
     9121 ctgggcctgg acagcaccgc tagcatcccg ctgctgaacg ctaaacagat tctgggacag
     9181 ctggacggcg ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg
     9241 gatcgaatcg gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg
     9301 agaggaagca gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg
     9361 ttttggcgcc tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc
     9421 atggcagaag cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac
     9481 ctgtatctgc aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc
     9541 gatgctgcac agatcgtcat tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa
     9601 agcgtggtca atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat
     9661 actctggccg ctatgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa
     9721 ggcctggctc catatctgtc acggtgggag aagctggata acttcatcaa tagacccgtg
     9781 aagctgatca ttggggacaa agagattttc gggattagcc gggggattga taaacaggga
     9841 gccctgctgc tggaacagga cggagttatc aaaccctgga tgggcggaga aatcagtctg
     9901 cggtctgccg aaaagtaagc caactttatt atacatagtt gatatccagc acagtggcgg
     9961 ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    10021 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    10081 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    10141 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    10201 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    10261 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    10321 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
    10381 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    10441 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    10501 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
    10561 ggaattcacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
    10621 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
    10681 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
    10741 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
    10801 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
    10861 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
    10921 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
    10981 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
    11041 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
    11101 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
    11161 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
    11221 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
    11281 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
    11341 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
    11401 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
    11461 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
    11521 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
    11581 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
    11641 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
    11701 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta
    11761 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
    11821 gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact
    11881 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctgcct agggaaccca
    11941 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
    12001 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
    12061 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
    12121 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    12181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    12241 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
    12301 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    12361 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgcg
    12421 tcgagacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
    12481 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
    12541 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    12601 gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat taagcgcggc
    12661 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    12721 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    12781 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    12841 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    12901 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    12961 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    13021 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
    13081 aatttcc
//
