LOCUS       CMV-10xPP7-OL           1439 bp    DNA     linear   UNA 16-JUL-2020
DEFINITION  .
ACCESSION   urn.local...g-c62k50o
VERSION     urn.local...g-c62k50o
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    122..597
                     /Source="referenceToDocument: urn:local:.:w-ams0y8t"
                     /Transferred_From="Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="Digestion of 10xPP7 - OL - Fragment 2 extraction
                     (concatenated sequence 2)"
                     /note="Geneious type: concatenated sequence"
     misc_feature    128..152
                     /Sequence="TATGGAGTTTATATGGAAACCCATA"
                     /Hairpin_Tm="47.2"
                     /Tm="55.3"
                     /Self_Dimer_Tm="21.3"
                     /%GC="32.0"
                     /created_by="primer3"
                     /Transferred_From="10xPP7 - OL, Digestion of 10xPP7 - OL -
                     Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="PP7-1"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    170..194
                     /Sequence="CAGAGATTTCATATGGGAAACTCTG"
                     /Hairpin_Tm="44.5"
                     /Tm="57.5"
                     /Self_Dimer_Tm="26.2"
                     /%GC="40.0"
                     /created_by="primer3"
                     /X-Transferred_From="10xPP7 - OL"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="10xPP7 - OL, 6xMS2 - 5xPP7 - OL,
                     Digestion of 10xPP7 - OL - Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="PP7-2"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    240..264
                     /Sequence="ATTCCAGTTTATATGGAAACGGAAT"
                     /Hairpin_Tm="45.1"
                     /Tm="56.9"
                     /Self_Dimer_Tm="21.3"
                     /%GC="32.0"
                     /created_by="primer3"
                     /Transferred_From="10xPP7 - OL, Digestion of 10xPP7 - OL -
                     Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="PP7-3"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    282..306
                     /Sequence="TAAGGAGTTTATATGGAAACCCTTA"
                     /Hairpin_Tm="42.2"
                     /Tm="55.2"
                     /Self_Dimer_Tm="21.2"
                     /%GC="32.0"
                     /created_by="primer3"
                     /X-Transferred_From="10xPP7 - OL"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="10xPP7 - OL, 6xMS2 - 5xPP7 - OL,
                     Digestion of 10xPP7 - OL - Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="pp7-wt"
                     /note="Geneious type: primer_bind_reverse"
     unsure          282..306
                     /created_by="User"
                     /X-Transferred_From="Naor Genscript order- Qbx5-PP7x4
                     cassette; Naor Genscript order- Qbx5-PP7x4 cassette
                     extraction; gblock-5xqb-4xms2"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="6xMS2 - 5xPP7 - OL, Naor Genscript
                     order- Qbx5-PP7x4 cassette, Naor Genscript order-
                     Qbx5-PP7x4 cassette extraction"
                     /Transferred_Similarity="100.00%"
                     /label="ms2-BS"
     misc_feature    336..360
                     /Sequence="AAAGGAGATTATATGAAATCCCTTT"
                     /Hairpin_Tm="43.1"
                     /Tm="54.2"
                     /Self_Dimer_Tm="18.8"
                     /%GC="28.0"
                     /created_by="primer3"
                     /Transferred_From="10xPP7 - OL, Digestion of 10xPP7 - OL -
                     Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="pp7-4"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    389..411
                     /Sequence="TACGAGTCAATATGGTGACCGTA"
                     /Hairpin_Tm="47.6"
                     /Tm="58.8"
                     /Self_Dimer_Tm="22.2"
                     /%GC="43.5"
                     /created_by="primer3"
                     /X-Transferred_From="10xPP7 - OL"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="10xPP7 - OL, 6xMS2 - 5xPP7 - OL,
                     Digestion of 10xPP7 - OL - Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="pp7-5"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    442..466
                     /Sequence="TAAGCACATTATAAGGAATGGCTTA"
                     /Hairpin_Tm="43.6"
                     /Tm="56.4"
                     /Self_Dimer_Tm="19.6"
                     /%GC="32.0"
                     /created_by="primer3"
                     /Transferred_From="10xPP7 - OL, Digestion of 10xPP7 - OL -
                     Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="pp7-6"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    484..508
                     /Sequence="ATTGGAGTAAATATGGTTACCCAAT"
                     /Hairpin_Tm="46.0"
                     /Tm="56.3"
                     /Self_Dimer_Tm="18.2"
                     /%GC="32.0"
                     /created_by="primer3"
                     /X-Transferred_From="10xPP7 - OL"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="10xPP7 - OL, 6xMS2 - 5xPP7 - OL,
                     Digestion of 10xPP7 - OL - Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="pp7-7"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    523..547
                     /Sequence="TTTCGACATTATATGGAATGCGAAA"
                     /Hairpin_Tm="47.0"
                     /Tm="57.7"
                     /Self_Dimer_Tm="23.2"
                     /%GC="32.0"
                     /created_by="primer3"
                     /X-Transferred_From="10xPP7 - OL"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="10xPP7 - OL, 6xMS2 - 5xPP7 - OL,
                     Digestion of 10xPP7 - OL - Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="pp7-8"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(549..567)
                     /Sequence="GCCAGGACGTGACGTTTCT"
                     /Hairpin_Tm="41.4"
                     /Tm="60.3"
                     /Self_Dimer_Tm="12.2"
                     /%GC="57.9"
                     /created_by="primer3"
                     /X-Transferred_From="10xMS2 - OL; 10xPP7 - OL; 6xMS2 -
                     5xQB - OL; Clipboard Contents 7; Naor Genscript order-
                     Qbx5-PP7x4 cassette; Naor Genscript order- Qbx5-PP7x4
                     cassette extraction"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="10xMS2 - OL, 6xMS2 - 5xPP7 - OL, 6xMS2
                     - 5xQB - OL, Naor Genscript order- Qbx5-PP7x4 cassette,
                     Naor Genscript order- Qbx5-PP7x4 cassette extraction"
                     /Transferred_Similarity="100.00%"
                     /label="NK_rev_pT7_5xQb-4xMS2 "
     misc_feature    568..592
                     /Sequence="TACGGAGTTTATATGGAAACCCGTA"
                     /Hairpin_Tm="49.1"
                     /Tm="59.4"
                     /Self_Dimer_Tm="27.0"
                     /%GC="40.0"
                     /created_by="primer3"
                     /Transferred_From="10xPP7 - OL, Digestion of 10xPP7 - OL -
                     Fragment 2, Ligated sequences"
                     /Transferred_Similarity="100.00%"
                     /label="pp7-9"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     614..631
                     /Sequence="CCGCTTCGAGCAGACATG"
                     /Hairpin_Tm="45.7"
                     /Tm="58.6"
                     /Self_Dimer_Tm="4.7"
                     /%GC="61.1"
                     /created_by="primer3"
                     /modified_by="User"
                     /Transferred_From="Digestion of pCMV-EcorI, Ligated
                     sequences, backup of pCMV-EcorI, pCMV-EcorI, pCMV-YFP,
                     pCMV-YFP - F-cmv-YFPdel-HindIII , pCMV-YFP extraction
                     noYFP, pCMV-empty, pCMV-empty extraction"
                     /Transferred_Similarity="100.00%"
                     /label="F-cmv-YFPdel-HindIII "
     polya_signal    624..>716
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pCMV-empty extraction"
                     /Transferred_Similarity="100.00%"
                     /label="SV40 polyA"
     polya_signal    624..>749
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pCMV-empty extraction"
                     /Transferred_Similarity="100.00%"
                     /label="SV40 polyA"
     primer_bind     complement(640..674)
                     /Sequence="GCATTCTAGTTGTGGTTTGTCCAAACTCATCAATG"
                     /Hairpin_Tm="48.9"
                     /Tm="66.7"
                     /Self_Dimer_Tm="18.2"
                     /%GC="40.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /Transferred_From="pCMV-EcorI"
                     /Transferred_Similarity="100.00%"
                     /label="R-sv40polyA"
     primer_bind     complement(644..663)
                     /Sequence="GTGGTTTGTCCAAACTCATC"
                     /Hairpin_Tm="48.9"
                     /Tm="54.8"
                     /Self_Dimer_Tm="9.1"
                     /%GC="45.0"
                     /created_by="primer3"
                     /note="SV40 polyA terminator, reverse primer"
                     /Transferred_From="Digestion of pCMV-EcorI, EF1A-MS2-3xBFP
                     AG 75384, EF1A-N22p-3xmCherry AG 75387 (modified),
                     EF1A-PP7-3xGFP AG 75385, Exported, Exported (modified),
                     Ligated sequences, backup of pCMV-EcorI, pCMV-YFP,
                     pCMV-YFP extraction noYFP, pCMV-empty, pCMV-empty
                     extraction"
                     /Transferred_Similarity="100.00%"
                     /label="EBV-rev; R-sv40polyA"
     primer_bind     complement(1009..1026)
                     /note="L4440 vector, forward primer"
                     /X-Transferred_From="; EF1A-MS2-3xBFP AG 75384;
                     EF1A-N22p-3xmCherry AG 75387 (modified); EF1A-PP7-3xGFP AG
                     75385; Exported; Exported (modified); singer-ms2x24"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From=", EF1A-MS2-3xBFP AG 75384,
                     EF1A-N22p-3xmCherry AG 75387 (modified), EF1A-PP7-3xGFP AG
                     75385, Exported, Exported (modified), singer-ms2x24"
                     /Transferred_Similarity="100.00%"
                     /label="L4440"
ORIGIN      
        1 acgtacactc cgcccattga cgcaatgggc ggtaggcgtg tacggtggga ggtctatata
       61 agcagagctg gtttatgaac cgtcagatcc gagctcctat ctagcgtcgc gttgacgaat
      121 tcttacatat ggagtttata tggaaaccca tacgcctgct ggtcgtgacc agagatttca
      181 tatgggaaac tctgcgagac aatgctacct taccgtcggc ccacttgttt ttacccatga
      241 ttccagttta tatggaaacg gaatcgcctg ctggtcgtga ctaaggagtt tatatggaaa
      301 cccttagaaa agccgtcgcc ttgaagccga gaacaaaagg agattatatg aaatcccttt
      361 atggattggg tctccaattc ctgtctgtta cgagtcaata tggtgaccgt acatcaggct
      421 tcgcagtatg caacgcttgc gtaagcacat tataaggaat ggcttattca ccgctttcct
      481 aagattggag taaatatggt tacccaatgt actaactcgc agtttcgaca ttatatggaa
      541 tgcgaaacag aaacgtcacg tcctggctac ggagtttata tggaaacccg taggaagaat
      601 tccccctaag cttccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
      661 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      721 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      781 gtttcaggtt cagggggagg tgttgggaag gttttttaaa gcagttaaaa tctctacaaa
      841 tgtggtaaga tccataagga tcgagtatgt caaccgatgc ccttgagagc cttcaaccca
      901 gtcatctcct tccggtgagc accgggcatg actatcatca ccgcacttat gactgtcgtc
      961 cgtagcaggc cactcgtacg acaggtaccg acatcgctct tccgcttcct cgctcactga
     1021 ctcgctgcgc tcggtcgttc ggctgcggca agcggtatca cctcactcaa aggcagtaat
     1081 acggttatcg acagaatcat gtggacatct catgaaggaa ctgtcgaccc aaaggccagc
     1141 aaaaagccag taaccgtaaa aaggccccgt gctggcgttt ttccaaagcc cccccccccc
     1201 ttactaacat ccaaaaatca cccctgaatt ccaaagtggc caaacccgaa gggctaagga
     1261 agcccggctt tttcccttga aaatccccgg gggcccttcc tgtgccaccc ctcctttacg
     1321 gaaacatatc cccttttccc tctggagagg gggaatttta aaaagagggg ggggaatttt
     1381 agttccgggg gggtgtaagc gggggttgtg acaacccccg ttttaaagaa gaatataag
//
