LOCUS       Exported                3263 bp ds-DNA     circular SYN 31-OCT-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Feb 14, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1
                     /label=start
                     /note="/dnas_title=start"
                     /note="/vntifkey=21"
     promoter        229..235
                     /label=Tet_promoter
                     /note="/dnas_title=Tet_promoter"
                     /note="/vntifkey=30"
     misc_feature    272..1462
                     /label=TetR
                     /note="/dnas_title=TetR"
                     /note="/vntifkey=21"
     primer_bind     1607..1626
                     /label=pTC14_F2 primer
                     /note="/dnas_title=pTC14_F2 primer"
                     /note="/vntifkey=28"
     misc_feature    1633
                     /label=end
                     /note="/dnas_title=end"
                     /note="/vntifkey=21"
     primer_bind     1703..1724
                     /label=pTC14_R1 primer
                     /note="/dnas_title=pTC14_R1 primer"
                     /note="/vntifkey=28"
     primer_bind     1749..1790
                     /label=Primer 1
     misc_feature    1754..1759
                     /label=BsaI site
                     /note="BsaI site"
     misc_feature    1761..1790
                     /label=PCR_primer
                     /note="132-tRNA-F"
     misc_feature    1765..1841
                     /label=tRNA
                     /note="dnas_title=tRNA
                     vntifkey=21"
     variation       1765..1770
                     /label=5' lead seq
                     /note="5' lead seq"
     misc_structure  1842..1874
                     /label=guide RNA cloning site
                     /note="guide RNA cloning site"
     stem_loop       1875..2010
                     /label=guide RNA scaffold 2.0
                     /note="guide RNA scaffold 2.0"
     primer_bind     complement(1985..2026)
                     /label=Primer 2
     primer_bind     complement(1985..2010)
                     /label=133-tRNA2.0-R
     misc_feature    2011..2014
                     /label=4nt
                     /note="4nt"
     misc_feature    2016..2021
                     /label=BsaI site
                     /note="BsaI site"
     misc_feature    2086..2759
                     /label=ori
                     /note="/dnas_title=ori"
                     /note="/vntifkey=21"
     primer_bind     3155..3174
                     /label=pTC14_F1 primer
                     /note="/dnas_title=pTC14_F1 primer"
                     /note="/vntifkey=28"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
      241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
      301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
      601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
      781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
      841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
     1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1741 tgcctcctct agaggtctcg ccagaacaaa gcaccagtgg tctagtggta gaatagtacc
     1801 ctgccacggt acagacccgg gttcgattcc cggctggtgc aagagacgag atctagtctg
     1861 agtcgaccgt ctctgtttta gagctaggcc aacatgagga tcacccatgt ctgcagggcc
     1921 tagcaagtta aaataaggct agtccgttat caacttggcc aacatgagga tcacccatgt
     1981 ctgcagggcc aagtggcacc gagtcggtgc tgttggagac cctcgagcca cccatgacca
     2041 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2221 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2761 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2821 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2881 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     2941 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3001 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3061 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3121 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3181 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3241 tggcagttcc ctactctcgc gtt
//
