LOCUS       pEMS2279        4731 bp    DNA     circular      1-DEC-2016
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11526505|
COMMENT     VNTDBDATE|-11526505|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2279|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|GenScript USA Inc|
COMMENT     VNTOAUTHORTEL|732-885-9188|
COMMENT     VNTOAUTHORFAX|732-210-0262|
COMMENT     VNTOAUTHOREML|gene@genscript.com|
COMMENT     VNTOAUTHORWWW|www.genscript.com|
COMMENT     VNTOAUTHORAD1|GenScript USA Inc|
COMMENT     VNTOAUTHORAD2|120 Centennial Ave|
COMMENT     VNTOAUTHORAD3|Piscataway, NJ 08854, USA|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEMS2279" 0 4731 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT         "NotI" "SalI" "SmaI" "EcoRV" "HindIII" "ScaI" "XbaI" "PstI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 4731 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AhdI" "gacnnnnngtc" 7 0 1 3745 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 1 2447 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AsiSI" "gcgatcgc" 6 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 2457 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 397 0 1610 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "FseI" "ggccggcc" 7 0 1 438 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "MluI" "acgcgt" 2 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 2470 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 2463 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2172 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2493 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 2007 0 4225 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 426 0 1133 0 0 "") 
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COMMENT             4432 0 4529 0 4559 0 4580 0 4592 0 4639 0 4676 0 4685 0 4690 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "gtg" "gtg" 1 0 83 13 0 22 0 118 0 128 0 177 0 
COMMENT             191 0 193 0 282 0 325 0 394 0 544 0 546 0 582 0 612 0 623 0 634 0 
COMMENT             903 0 905 0 930 0 1050 0 1176 0 1240 0 1284 0 1388 0 1473 0 1486 0 
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COMMENT             0 4079 0 4140 0 4212 0 4219 0 4250 0 4326 0 4411 0 4460 0 4653 0 0 
COMMENT             "") 
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COMMENT             536 0 564 0 643 0 647 0 665 0 752 0 781 0 986 0 1222 0 1290 0 1346 
COMMENT             0 1589 0 1694 0 1705 0 1810 0 2028 0 2057 0 2147 0 2177 0 2313 0 
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COMMENT             0 3168 0 3240 0 3253 0 3266 0 3344 0 3434 0 3553 0 3596 0 3694 0 
COMMENT             3817 0 3823 0 4003 0 4147 0 4165 0 4306 0 4406 0 4414 0 4445 0 4510 
COMMENT             0 4637 0 4657 0 4715 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "taa" "taa" 1 0 87 72 0 143 0 214 0 340 0 349 
COMMENT             0 380 0 441 0 448 0 466 0 630 0 658 0 668 0 697 0 705 0 716 0 724 0 
COMMENT             735 0 771 0 775 0 778 0 843 0 851 0 872 0 913 0 919 0 938 0 1007 0 
COMMENT             1041 0 1094 0 1116 0 1143 0 1164 0 1179 0 1187 0 1194 0 1352 0 1404 
COMMENT             0 1563 0 1661 0 1697 0 1701 0 1897 0 2109 0 2115 0 2121 0 2252 0 
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COMMENT             0 2809 0 2837 0 2888 0 2991 0 3209 0 3235 0 3272 0 3332 0 3558 0 
COMMENT             3610 0 3615 0 3629 0 3639 0 3654 0 3682 0 3757 0 3847 0 3917 0 3940 
COMMENT             0 3956 0 4127 0 4172 0 4198 0 4295 0 4321 0 4401 0 4501 0 4616 0 
COMMENT             4668 0 4693 0 4700 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "tta" "tta" 1 0 80 142 0 300 0 339 0 440 0 465 
COMMENT             0 499 0 541 0 573 0 629 0 657 0 678 0 685 0 723 0 727 0 738 0 742 0 
COMMENT             774 0 819 0 842 0 856 0 859 0 865 0 894 0 1102 0 1115 0 1169 0 1351 
COMMENT             0 1357 0 1542 0 1577 0 1580 0 1604 0 1617 0 1723 0 1908 0 1945 0 
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COMMENT             0 4060 0 4093 0 4143 0 4156 0 4180 0 4320 0 4374 0 4438 0 4550 0 
COMMENT             4562 0 4571 0 4607 0 4678 0 4681 0 4692 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "tga" "tga" 1 0 86 14 0 17 0 23 0 33 0 172 0 
COMMENT             194 0 395 0 624 0 931 0 1062 0 1066 0 1148 0 1215 0 1241 0 1277 0 
COMMENT             1302 0 1370 0 1378 0 1389 0 1395 0 1408 0 1426 0 1497 0 1515 0 1567 
COMMENT             0 1574 0 1601 0 1608 0 1635 0 1673 0 1764 0 1770 0 1792 0 1800 0 
COMMENT             1806 0 1830 0 1845 0 1869 0 1949 0 1979 0 2049 0 2102 0 2142 0 2154 
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COMMENT             3802 0 4065 0 4213 0 4220 0 4242 0 4384 0 4422 0 4461 0 4521 0 4554 
COMMENT             0 4582 0 4598 0 4661 0 4687 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "tca" "tca" 1 0 89 58 0 101 0 111 0 156 0 232 
COMMENT             0 250 0 369 0 445 0 484 0 535 0 577 0 591 0 646 0 795 0 805 0 869 0 
COMMENT             970 0 1010 0 1019 0 1026 0 1072 0 1089 0 1221 0 1429 0 1588 0 1613 
COMMENT             0 1655 0 1850 0 1940 0 1963 0 1993 0 2027 0 2075 0 2146 0 2173 0 
COMMENT             2233 0 2286 0 2382 0 2505 0 2511 0 2545 0 2617 0 2637 0 2746 0 2790 
COMMENT             0 2795 0 2799 0 2829 0 2942 0 2958 0 2963 0 3104 0 3111 0 3127 0 
COMMENT             3182 0 3497 0 3536 0 3552 0 3571 0 3582 0 3595 0 3633 0 3685 0 3702 
COMMENT             0 3723 0 3822 0 3840 0 4002 0 4026 0 4030 0 4050 0 4120 0 4146 0 
COMMENT             4150 0 4186 0 4227 0 4236 0 4284 0 4330 0 4333 0 4365 0 4428 0 4444 
COMMENT             0 4528 0 4544 0 4565 0 4579 0 4684 0 4714 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "tag" "tag" 1 0 47 427 0 491 0 500 0 542 0 574 
COMMENT             0 597 0 679 0 686 0 743 0 822 0 866 0 888 0 916 0 1082 0 1103 0 
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COMMENT             3443 0 3600 0 3730 0 3753 0 3935 0 3944 0 3959 0 4094 0 4248 0 4310 
COMMENT             0 4608 0 4624 0 4706 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "cta" "cta" 1 0 41 146 0 297 0 426 0 566 0 596 
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COMMENT             3714 0 3760 0 3913 0 3934 0 3985 0 4667 0 4697 0 0 "")) 
COMMENT       (CObList 
COMMENT        #26=(CFSignal (CObList) "bla(ApR)" 4 0 1 3671 4531 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3671..4531)") 
COMMENT        #27=(CFSignal (CObList) "rep(pMB1)" 33 0 1 2897 3511 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2897..3511)") 
COMMENT        #28=(CFSignal (CObList) "Ple349" 21 0 0 432 2452 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "432..2452")) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA Plasmid 'pEMS2279'" 1) 
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COMMENT                          "Currently local object. Original author: GenScript USA Inc" 
COMMENT                          1) 
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COMMENT                          "Created: 12/01/16 10:40AM" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 12/01/16 10:40AM" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4731 bp" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 29-NOV-2016" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "Piscataway, NJ 08854, USA" 1) 
COMMENT                     #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "TEL: 732-885-9188  FAX: 732-210-0262" 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:gene@genscript.com\">gene@genscript.com</a>" 
COMMENT                          1) 
COMMENT                     #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "WWW: <a href=\"http://www.genscript.com\">www.genscript.com</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                   "Annotations") 
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COMMENT                                        "Start: 3671  End: 4531 (Complementary)" 
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COMMENT                                        "   complement(3671..4531)" 1))) 
COMMENT                                 "bla(ApR)"))) "CDS (1 total)") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 432   End: 2452" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   432..2452" 1))) "Ple349"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                        "   complement(2897..3511)" 1))) 
COMMENT                                 "rep(pMB1)"))) "Replication Origin (1 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                          "GACNNNNNGTC" "CTGNNNNNCAG") 
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COMMENT                          "GATATC" "CTATAG") 
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FEATURES             Location/Qualifiers
     CDS             complement(3671..4531)
                     /vntifkey="4"
                     /label=bla(ApR)
     rep_origin      complement(2897..3511)
                     /vntifkey="33"
                     /label=rep(pMB1)
     misc_feature    432..2452
                     /vntifkey="21"
                     /label=Ple349
BASE COUNT     1263 a      1033 c      1175 g      1260 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa 
      421 tgcatctaga tggccggcct taattcataa gtttttttcc aagattaatg ttgttctgga 
      481 gaatcaaacg tagggtattt agggaaagag gggccaggct tgtacattcc tttttcacgt 
      541 ttagtgtgtt cccctctgga gtccactatg cattagtcag agtggaaagt tcagtctagg 
      601 agattccaaa tgtggaggaa atgtgacttt aatgtgtctt cccactcact ggtattttaa 
      661 gaagcactaa agaaagatta gagattagcg cttgcataag cagctaaagg acagctaaat 
      721 ttttaattac tttctaatta cttagtacct gcactctact ttccaatata taattaataa 
      781 cacttttcca tctgtcagaa gggatcaaga accagaagtt atagagctat ggaatacaga 
      841 tttaagaaaa taattttatt atatttagtc ataaaatttg catacagtag agcttactcc 
      901 ttgtgtggta tgtaatagta aaggaagatg tgaatggtaa tggagataca gaggagggag 
      961 cagagggact catccagaga ccaaacactt tcctggcttt gcaaggtaat cattgctttc 
     1021 aaaactcaga ggaattttgg taaagaccag tggagagcat atgaatgact ctcaagctgt 
     1081 ctagttgctc atgtaatctg cttagggctg caggttaaga acctctccct gtctagaaag 
     1141 gataaattga ccttctttct ctgtaatgtt atggagtgta attttctaat aggtaacaac 
     1201 tggggaaaga acattgaaat tcactggcaa ggcatggaag tgaccagcaa acagacatct 
     1261 tggtctctgt ctgctttgat gtagtgcagc acaaagacaa ttgaatatgc aaggtacttc 
     1321 cagatttggg ccctgttcct ggctccacta ttaattttat ttgcagtctt gaatagctga 
     1381 acttctggtg aatttgagca ggataattga agggctttgg ggggttgatc aggttccaaa 
     1441 agaaaaattg cagaagaaaa catgttggac ctgtgggcct gggctgtggc tggggatgaa 
     1501 aatgggaaag aacatgaagg agatacatgc agaccttctt gttatgccat gggacaagca 
     1561 aataagtgaa acttgattat tacaaggtca ctgcaaaact tgattactga attcatttag 
     1621 aaggagccca atagtgacct ccctgtttgc agcttcatag taaacagttt tctgaagaag 
     1681 gaggaatctg tggcactaaa taaccacaga gaaaacagtt ctttactccc aggatgtgcc 
     1741 tgcaaacttg gcccaggatg gtgtgagtgt gaggaaaagg agaaggggag ctgagagcat 
     1801 gagaatgacc acagggcagg gcaggtggct gagccttctc cctttgaggt caagtgtttg 
     1861 ttccattctg aagcagggtg ctggctggaa ttttggtaat ttttttttta tggctttggt 
     1921 ttgggtcttg ctttgctttt caaattattg attttctctg cctcagcagg accctgggtg 
     1981 atatttatgg gctcaacccc agcagtactt cccttcccag ctcctttcac tgtgtatatg 
     2041 tgtgtgtgtg attgcgcaca taggtgtgtg tttgtcagag aaagggaaga aatgggggta 
     2101 ttgaaatcta aaggtaagaa taaaaaaaag acagaagata ttgaatcaca aactgaaatt 
     2161 tacccttgga tatcaacact ttttgtgttt ttgtgaatac aatacctatt tattgactgg 
     2221 aatgaaccca agtcagtttt gaaagggcct ctaattattg gaaaactcct aagcctgtcc 
     2281 cagcatcaac ccatgttctc ctaacttctt tgcacctggg ttgctgttct gagcttatga 
     2341 cggtgagatt aggcagcatg agggtattac agagagataa gtcaggatgc aaagatcctt 
     2401 ttgtcttttg caaagaaggc agctgggcag ctgataggaa caacggcgcg ccatcggatc 
     2461 ccgggcccgt cgactgcaga ggcctgcatg caagcttggc gtaatcatgg tcatagctgt 
     2521 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     2581 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     2641 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     2701 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     2761 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     2821 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     2881 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     2941 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     3001 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     3061 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     3121 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     3181 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     3241 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     3301 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 
     3361 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     3421 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     3481 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     3541 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     3601 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     3661 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     3721 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     3781 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     3841 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     3901 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     3961 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     4021 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     4081 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     4141 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     4201 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     4261 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     4321 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     4381 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     4441 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     4501 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     4561 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     4621 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 
     4681 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c 
//
