LOCUS       Exported                5363 bp ds-DNA     circular SYN 17-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Feb 14, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    6..33
                     /label=FUS_A_left
     misc_feature    21
                     /label=CUT 1
     misc_feature    complement(49..324)
                     /label=lacZ'
     promoter        complement(325..446)
                     /label=lacZ promoter
     primer_bind     460..497
                     /label=Primer 1
     primer_bind     460..497
                     /label=Primer 1
     misc_feature    463..475
                     /label=FUS_A_right
                     /note="FUS_A_right"
     misc_feature    469..505
                     /label=PCR_primer
                     /note="132-tRNA-F"
     primer_bind     469..496
                     /label=Primer 1
     misc_feature    469..474
                     /label=BsaI site
                     /note="BsaI site"
     misc_feature    480..556
                     /label=tRNA
                     /note="dnas_title=tRNA
                     vntifkey=21"
     variation       480..485
                     /label=5' lead seq
                     /note="5' lead seq"
     primer_bind     complement(541..576)
                     /label=Primer 2
     primer_bind     complement(541..562)
                     /label=Primer 2
     misc_feature    562..579
                     /label=pT
     misc_feature    594..693
                     /label=attL2
                     /note="dnas_title=attL2 ApEinfo_fwdcolor=#ff0080 
                     vntifkey=21 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 
                     -1} {} 0} width 5 offset 0 ApEinfo_revcolor=#ff0080"
     primer_bind     complement(710..731)
                     /label=T7
                     /note="dnas_title=T7 ApEinfo_fwdcolor=cyan vntifkey=28 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=green"
     primer_bind     complement(730..750)
                     /label=M13-rev
                     /note="dnas_title=M13-rev ApEinfo_fwdcolor=cyan vntifkey=28
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=green"
     misc_feature    complement(734..750)
                     /label=M13-R
                     /note="dnas_title=M13-R ApEinfo_fwdcolor=cyan vntifkey=21 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=cyan"
     misc_feature    828..961
                     /label=Spn promoter
                     /note="dnas_title=Spn promoter ApEinfo_fwdcolor=#2fec13 
                     vntifkey=21 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 
                     -1} {} 0} width 5 offset 0 ApEinfo_revcolor=#2fec13"
     misc_feature    962..1972
                     /label=SpnR
                     /note="dnas_title=SpnR ApEinfo_fwdcolor=#f70938 vntifkey=21
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=#f70938"
     misc_feature    2039..2712
                     /label=pUC origin
                     /note="dnas_title=pUC origin ApEinfo_fwdcolor=cyan 
                     vntifkey=21 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 
                     -1} {} 0} width 5 offset 0 ApEinfo_revcolor=green"
     primer_bind     3251..3268
                     /label=M13-fwd
                     /note="dnas_title=M13-fwd ApEinfo_fwdcolor=cyan vntifkey=28
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=green"
     misc_feature    3252..3267
                     /label=M13-F
                     /note="dnas_title=M13-F ApEinfo_fwdcolor=cyan vntifkey=21 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_revcolor=cyan"
     misc_feature    3270..3382
                     /label=attL5
                     /note="vntifkey=21
                     dnas_title=attL5
                     ApEinfo_revcolor=#c0c0c0
                     ApEinfo_fwdcolor=#c0c0c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0"
     promoter        3400..5362
                     /label=Ubi1 (maize ubiquitin promoter 1)
                     /note="Ubi1 (maize ubiquitin promoter 1)"
ORIGIN
        1 gatccccaag tggtggctat cgagaccggc gccgctacag ggcgcgtccc attcgccatt
       61 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      121 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      181 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
      241 gggtaccggg ccccccctcg aggtcctcca gcttttgttc cctttagtga gggttaattg
      301 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccaccggtgg tctcttgtta
      481 acaaagcacc agtggtctag tggtagaata gtaccctgcc acggtacaga cccgggttcg
      541 attcccggct ggtgcaacta gtttttttgc tagcagatct aagggcgaat tcgacccagc
      601 tttcttgtac aaagttggca ttataaaaaa taattgctca tcaatttgtt gcaacgaaca
      661 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
      721 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
      781 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctggacca gccaggacag
      841 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
      901 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
      961 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     1021 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     1081 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     1141 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     1201 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     1261 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     1321 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     1381 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     1441 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     1501 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     1561 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     1621 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     1681 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     1741 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     1801 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     1861 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     1921 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
     1981 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     2041 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2101 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2161 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     2221 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2281 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2341 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2401 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     2461 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2521 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2581 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2641 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2701 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2761 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2821 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     2881 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2941 taatacgcgt accgctcgcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
     3001 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     3061 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     3121 gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt
     3181 tttatttgat gcctggcagt tccctactct cgcgttaacg cttgcatgga tgttttccca
     3241 gtcacgacgt tgtaaaacga cggccagtct taagctcggg cccaaataat gattttattt
     3301 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
     3361 actttgtata caaaagttgc cccatggcgt tccctctaga gagataatga gcattgcatg
     3421 tctaagttat aaaaaattac cacatatttt ttttgtcaca cttgtttgaa gtgcagttta
     3481 tctatcttta tacatatatt taaactttac tctacgaata atataatcta tagtactaca
     3541 ataatatcag tgttttagag aatcatataa atgaacagtt agacatggtc taaaggacaa
     3601 ttgagtattt tgacaacagg actctacagt tttatctttt tagtgtgcat gtgttctcct
     3661 ttttttttgc aaatagcttc acctatataa tacttcatcc attttattag tacatccatt
     3721 tagggtttag ggttaatggt ttttatagac taattttttt agtacatcta ttttattcta
     3781 ttttagcctc taaattaaga aaactaaaac tctattttag tttttttatt taataattta
     3841 gatataaaat agaataaaat aaagtgacta aaaattaaac aaataccctt taagaaatta
     3901 aaaaaactaa ggaaacattt ttcttgtttc gagtagataa tgccagcctg ttaaacgccg
     3961 tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag
     4021 cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg
     4081 ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg
     4141 gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc
     4201 caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc
     4261 ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa
     4321 tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc ccccccctct
     4381 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt
     4441 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat
     4501 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat
     4561 cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt
     4621 tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc
     4681 gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt
     4741 cgttctagat cggagtagaa ttaattctgt ttcaaactac ctggtggatt tattaatttt
     4801 ggatctgtat gtgtgtgcca tacatattca tagttacgaa ttgaagatga tggatggaaa
     4861 tatcgatcta ggataggtat acatgttgat gcgggtttta ctgatgcata tacagagatg
     4921 ctttttgttc gcttggttgt gatgatgtgg tgtggttggg cggtcgttca ttcgttctag
     4981 atcggagtag aatactgttt caaactacct ggtgtattta ttaattttgg aactgtatgt
     5041 gtgtgtcata catcttcata gttacgagtt taagatggat ggaaatatcg atctaggata
     5101 ggtatacatg ttgatgtggg ttttactgat gcatatacat gatggcatat gcagcatcta
     5161 ttcatatgct ctaaccttga gtacctatct attataataa acaagtatgt tttataatta
     5221 ttttgatctt gatatacttg gatgatggca tatgcagcag ctatatgtgg atttttttag
     5281 ccctgccttc atacgctatt tatttgcttg gtactgtttc ttttgtcgat gctcaccctg
     5341 ttgtttggtg ttacttctgc agg
//
