LOCUS       Exported                5353 bp ds-DNA     circular SYN 08-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Feb 14, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        13..1975
                     /label=Ubi1 (maize ubiquitin promoter 1)
                     /note="Ubi1 (maize ubiquitin promoter 1)"
     misc_feature    1982..2009
                     /label=FUS_A_left
                     /note="FUS_A_left"
     misc_feature    1997
                     /label=CUT 1
                     /note="CUT 1"
     misc_feature    complement(2025..2300)
                     /label=lacZ'
                     /note="lacZ'"
     promoter        complement(2301..2422)
                     /label=lacZ promoter
                     /note="lacZ promoter"
     primer_bind     2436..2473
                     /label=Primer 1
     primer_bind     2436..2473
                     /label=Primer 1
     primer_bind     2436..2473
                     /label=Primer 1
     misc_feature    2439..2451
                     /label=FUS_A_right
                     /note="FUS_A_right"
     misc_feature    2439..2451
                     /label=FUS_A_right
                     /note="FUS_A_right"
     misc_feature    2445..2481
                     /label=PCR_primer
                     /note="132-tRNA-F"
     primer_bind     2445..2472
                     /label=Primer 1
     misc_feature    2445..2450
                     /label=BsaI site
                     /note="BsaI site"
     misc_feature    2456..2532
                     /label=tRNA
                     /note="dnas_title=tRNA
                     vntifkey=21"
     variation       2456..2461
                     /label=5' lead seq
                     /note="5' lead seq"
     primer_bind     complement(2515..2552)
                     /label=Primer 2
     primer_bind     complement(2517..2552)
                     /label=Primer 2
     primer_bind     complement(2517..2538)
                     /label=Primer 2
     misc_feature    2538
                     /label=pT
     misc_feature    2552..2651
                     /label=attL2
     misc_feature    2552
                     /label=attL2
                     /note="dnas_title=attL2 ApEinfo_fwdcolor=#ff0080 
                     vntifkey=21 ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 
                     -1} {} 0} width 5 offset 0 ApEinfo_revcolor=#ff0080"
     primer_bind     complement(2668..2689)
                     /label=T7
     primer_bind     complement(2688..2708)
                     /label=M13-rev
     misc_feature    complement(2692..2708)
                     /label=M13-R
     misc_feature    2786..2919
                     /label=Spn promoter
     misc_feature    2920..3930
                     /label=SpnR
     misc_feature    3997..4670
                     /label=pUC origin
     primer_bind     5209..5226
                     /label=M13-fwd
     misc_feature    5210..5225
                     /label=M13-F
     misc_feature    5228..5340
                     /label=attL5
ORIGIN
        1 ctagataacg caagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat
       61 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt
      121 tactctacga ataatataat ctatagtact acaataatat cagtgtttta gagaatcata
      181 taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac
      241 agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata
      301 taatacttca tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata
      361 gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa
      421 aactctattt tagttttttt atttaataat ttagatataa aatagaataa aataaagtga
      481 ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt
      541 ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag
      601 cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc
      661 ctctggaccc ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca
      721 gaaattgcgt ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct
      781 cacggcaccg gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg
      841 cccgccgtaa taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg
      901 agcgcacaca cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag
      961 gtacgccgct cgtcctcccc cccccccccc tctctacctt ctctagatcg gcgttccggt
     1021 ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg
     1081 ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga
     1141 ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag
     1201 acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc
     1261 tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt
     1321 cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattaattc
     1381 tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat
     1441 tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt
     1501 gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg
     1561 tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta
     1621 cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga
     1681 gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact
     1741 gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta
     1801 tctattataa taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg
     1861 gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc
     1921 ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagggatc
     1981 cccaagtggt ggctatcgag accggcgccg ctacagggcg cgtcccattc gccattcagg
     2041 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     2101 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     2161 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     2221 accgggcccc ccctcgaggt cctccagctt ttgttccctt tagtgagggt taattgcgcg
     2281 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     2341 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2401 actcacatta attgcgttgc gctcactgcc cgctttccac cggtggtctc tcggtaacaa
     2461 agcaccagtg gtctagtggt agaatagtac cctgccacgg tacagacccg ggttcgattc
     2521 ccggctggtg caactagtaa gggcgaattc gacccagctt tcttgtacaa agttggcatt
     2581 ataaaaaata attgctcatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2641 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     2701 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2761 aatatatcat catgcctcct ctggaccagc caggacagaa atgcctcgac ttcgctgctg
     2821 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca
     2881 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     2941 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     3001 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     3061 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     3121 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     3181 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     3241 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     3301 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     3361 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     3421 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     3481 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     3541 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     3601 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     3661 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     3721 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     3781 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     3841 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     3901 ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt
     3961 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4021 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4081 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4141 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     4201 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4261 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4321 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4381 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     4441 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4501 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4561 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4621 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4681 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4741 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4801 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4861 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctcgccag
     4921 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     4981 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     5041 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     5101 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     5161 cctactctcg cgttaacgct tgcatggatg ttttcccagt cacgacgttg taaaacgacg
     5221 gccagtctta agctcgggcc caaataatga ttttattttg actgatagtg acctgttcgt
     5281 tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtataca aaagttgccc
     5341 catggcgttc cct
//
