LOCUS       pEMS2166        4689 bp    DNA     circular      7-JUL-2016
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11315099|
COMMENT     VNTDBDATE|-11315099|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEMS2166|
COMMENT     VNTAUTHORNAME|Siu Ling Lam|
COMMENT     VNTAUTHORTEL|6048753877|
COMMENT     VNTAUTHOREML|sling@cmmt.ubc.ca|
COMMENT     VNTOAUTHORNAME|GenScript USA Inc|
COMMENT     VNTOAUTHORTEL|732-885-9188|
COMMENT     VNTOAUTHORFAX|732-210-0262|
COMMENT     VNTOAUTHOREML|gene@genscript.com|
COMMENT     VNTOAUTHORWWW|www.genscript.com|
COMMENT     VNTOAUTHORAD1|GenScript USA Inc|
COMMENT     VNTOAUTHORAD2|120 Centennial Ave|
COMMENT     VNTOAUTHORAD3|Piscataway, NJ 08854, USA|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 2419 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 469 0 2415 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 397 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 1669 0 2451 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 2437 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 2421 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 2419 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "PleI" "gagtc" 10 11 6 678 1 1296 1 2076 1 
COMMENT             2704 1 3189 0 3692 1 0 "") 
COMMENT        #11=(CRSite (CStringList) "SpeI" "actagt" 2 0 2 2064 0 2225 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 413 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 2428 0 0 "")) 
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COMMENT        #15=(CFSignal (CObList) "rep(pMB1)" 33 0 1 2855 3469 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "Ple331" 21 0 0 414 2410 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: GenScript USA Inc" 
COMMENT                          1) 
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COMMENT                          "Created: 07/07/16 04:14PM" 1) 
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COMMENT                          "length: 4689 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 17-MAY-2016" 1))) 
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COMMENT                          "EMAIL: <a href=\"mailto:gene@genscript.com\">gene@genscript.com</a>" 
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COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 
COMMENT       (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     CDS             complement(3629..4489)
                     /vntifkey="4"
                     /label=bla(ApR)
     rep_origin      complement(2855..3469)
                     /vntifkey="33"
                     /label=rep(pMB1)
     misc_feature    414..2410
                     /vntifkey="21"
                     /label=Ple331
BASE COUNT     1305 a      1062 c      1090 g      1232 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accggccggc 
      421 ctcccttttg ctacaggcat agtttaagtc ttgtgtcaca aataaaagga tcctccctag 
      481 catatggagt agttgccatg tcctcccagg gatggtctgt agctatgttc acctcaggct 
      541 catcaagaag aggtaaactg tcaagtataa tttcagtcat agggtggttt ggttgagaca 
      601 gattagtggt atgcaatcat gatagatagt ttatcccagc tccaaactgc cgcttgggaa 
      661 atcttgaact caaatgaaca gagactcagt cattattagt gtcaaaaagg tatacagagg 
      721 caaaatttgg ttggcagcat aaaagatgaa tcaaagcaaa aaagtgacca attgcatgtt 
      781 caaaatcgaa attgagatgc atactacagc ttggaaatac tttaaatacc agaaaagtca 
      841 cacattatta attgcgcatt gattaatatt taaaacatgt ttctgtaatg tattttcaaa 
      901 gagacaataa aattacatgt attttcttat ttcaaaacct ttattctcaa ggtgatgcat 
      961 agtaaggagt acccttttag atacaggagt aagatagcaa aatgtttttt aaaaatttgt 
     1021 ttaattttta gtatctttcc ttcagctgca tatttccaga aataagactt tttagaagaa 
     1081 gaatttagtt ttatgggact attttaatat tcaaatgtct tacagtttga tgactttctc 
     1141 aggaagaaat caggttataa taaaagcatg aatttaaaat gtggaagaac ctaatctaaa 
     1201 taactttcca aatattttct aaacactgaa aaatatatat ttataaattg taaaatacat 
     1261 tattctatta gcaaacacaa actttggtat ctttaaatca gactcccccc ccaaccaaat 
     1321 atccacaaaa agaatgtcct caagtgtcaa tgcatgaagt catgcgtgaa gataaatcta 
     1381 acagctcttt ctgtgtcatt cctaatgcac ttgtcactca gtattatcca tcctcattaa 
     1441 tgacaatggg aaagtttatc ttgggaacac gttttaaatc atctactctt tacaatggga 
     1501 ccatgaaatc tcttacaaaa catgaggcgg gaactactct gacaacaaaa ccccttcctg 
     1561 gcagctttat tcttaattcc tgttcaagga gtaatctgtt tattataaaa tcatacagaa 
     1621 caatatatta aacagaattt gaaacaagaa tagctagtgt gatttccaag cttacaagta 
     1681 tttttacaac atttttatta ggctgaaaac actacattga gttctttttg tgtgatttca 
     1741 tgcaatcttc tattctacat cgtaaacacc tttgaaaaga agttttgttt tacataattt 
     1801 aaaaataatt ttcaaatggt aaggacactg gtgtttatat aattatttta aaatacaata 
     1861 acttacatta gcttaaataa aagcagttat taagtaaaaa tgtgctcatg tttaaatttc 
     1921 ctaaccaaaa tgccaaaata ttccaactct tggggatcgg aggaggggac agggtgatta 
     1981 cccagagagg tagctggcca gcctaagggc agagatcttg gggccctagt gcccgaaggt 
     2041 gcggaggagc gcactcggca agactagttt cctggggatc gactctacgc catacaggac 
     2101 ggcggcccag gctggaccgg gccgggctag agcagtcaca ggccgggcca aggaaggcca 
     2161 aagcaggggt tggagccggc cggaccctgg gtggggagaa gcaggctccc gcccggccgg 
     2221 aaaactagtc ggcgcagagc tgtgcccaac tctagccgcc atgacgtcac gcgggccggg 
     2281 cagccaatga ggacggcgct ggcgtggata ttaaggaaag ttagcgcctg cctgagcacc 
     2341 ctcttttctt atcattgaca tttaaactct ggggcaggtc ctcgcgtaga acgcggctgt 
     2401 caggcgcgcc atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggcgt 
     2461 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     2521 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 
     2581 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
     2641 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
     2701 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     2761 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     2821 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     2881 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     2941 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     3001 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     3061 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     3121 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     3181 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     3241 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     3301 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     3361 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     3421 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     3481 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     3541 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     3601 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     3661 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     3721 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     3781 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     3841 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     3901 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     3961 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     4021 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     4081 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     4141 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     4201 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     4261 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     4321 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     4381 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     4441 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     4501 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     4561 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
     4621 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 
     4681 cctttcgtc 
//
