LOCUS       pZA3_pCR8_AtZAR1_L17E_S 5401 bp ds-DNA     circular     10-JUL-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_binding    3317..3341
                     /label="GW2 priming site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      4751..5339
                     /label="pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3648..4658
                     /label="Spectinomycin resistance gene"
                     /ApEinfo_revcolor=#dd0000
                     /ApEinfo_fwdcolor=#dd0000
     misc_binding    607..631
                     /label="GW1 priming site"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      complement(427..470)
                     /label="rrnB T1 transcription termination sequence"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3280..3379
                     /label="attL2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      complement(268..295)
                     /label="rrnB T2 transcription termination sequence"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(3396..3415)
                     /label="T7"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(569..668)
                     /label="attL1"
                     /ApEinfo_revcolor=#dd0000
                     /ApEinfo_fwdcolor=#dd0000
     cds             complement(697..3255)
                     /label="AtZAR1_L17E with STOP codon"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTCC GAATTCGCCC TTAATGGTCT CTAAGCTTAG GTTCTGTGCA ATGGTGTTTT
      721 CATCCACACT CCGCCTCTAA AGCCAACATC TTCAATCGCG AAAGACTCTA GCTCTGGACA
      781 CCAGTTTGCG GTCACGGTCC TCAGGTACGG CATTGACTGT TGCAGCACTT CCCAGTCCAT
      841 GTCTAGGTCC GACAATGAAC TTAACATTAA ACCTTCAATT CTCCAGTGAG TATTCTCGTT
      901 TCCCCAGAAT GGTTCCTGCA TTTTCACCAA ATTACCTGAA CATATTGACA TGTACCTCAA
      961 CATCGGAAGC TTGTGAGGAC TCAACCACGA TGGACTCGAC TTTCCAGGAT AGAATTGTAG
     1021 AGAAAGCTCA TGGAGCTGGT GTGGAGGAGT AAGGGCGTCT ATTTTGGTTA TGAGATCATC
     1081 GCCGTAGCTA TCATAGCAGT TGATCGAAAT GGACATCAGC TTGCTTAGGT TTATCAAAGA
     1141 ATCAAGCTCC TCCTCTTCTA TCTGATCGCC ACGAGTCAGA GAAAGACCAA GTTTCCGAAG
     1201 GTTTGTGAGA TTTTTCACTT CACTGAGCTT GCACCCATTG TTGGACCTGG CTGGCTTAAA
     1261 TCCCAACAGA ACTTCGAGCT TCACGAGACT TCCGATACCC TTGGGGAAGC ATTCAAGGGA
     1321 TCCACAGTTC GTCATGTCAA GTACAAGGAG TTTCTTGAAC AAGACGATGC AAGGTTGAAG
     1381 TTGTTTCAGA TTCTGACAGT AGCTTGCGTC TAGAATCTGA AGGTTGTGGA GATCTTCCAT
     1441 GGAACGAGGA AACTGGATCA ACGGATGTGT GTTGCTCAAA CTAAGACATG CTAAGTGTTG
     1501 AAGGCTTGCA ATCTCATCCA AAATCTCAGA CAGAGGAGCA TCAAATATGG ATTTCGAGAT
     1561 ATCGAGAACC CGAAGATATT TGCAGTCTGT GAATTTCTTA GCTAAGTCTG AGTTGAGCTT
     1621 GTTCACTTCA CCTGTCTTAG TTGTTGACAC TACGCCTCTC AGCTTATGGT TAACTTTGAT
     1681 CTGCTTCTCA TCAAAGTTTC CACTAATCCC AAGGTGACGA CAGTTGAGGC CTTCCGGGTT
     1741 AGAGAACGAA TCCTTCTTGG CTATATCGAT CACCAAATCA CGAACCATGT CATGAATCTT
     1801 GCAAGTGATG ATGGTTCCGC TATAGGTTTT GTCTACAACT TCTATTAGAC ACCTGTTTGT
     1861 GAGCCCGGAA AAGCAGTCCT CTCCAGATTC AGTTGCAGAT CTTCCGTTTC TCCACATGAC
     1921 AAAACCTTCT CCGATCCATC CATGTACTAG TTGTTGTTTT GGTATAACAC AGTCCTCTGG
     1981 ATAGAGCGAA AGCGTGAGAA TGCACGACTT AAGGTGGGAA GGGAGTTCAT CGTAGCTCAA
     2041 CTGCAGAGAT GACATAACAT TGTCAGTTTC AGAAGTGTTT CCTCTTAGCT CATCCTGAAA
     2101 ATGCTCTGCA ATTCGTCTCC ATTCATGATA GACATGGTCT TTGCACAGAA GTAACCCACC
     2161 AACCGCTTTG ATTGTCAAAG GCAAACCTTT GCATTTCGTT ACAATCTCCT TGCCAACATC
     2221 CTCTAGCTCG GGACGCTCAC AAGTACCGTC ATTTGCTGCA AATGCCACAT TACAAAACAG
     2281 CAGCCAACTG TTATCAGGAC TAAGGAGCTC TGGACGGTGA GTCTTGTCGT CTCTCGCTTG
     2341 CACCCTTTTC GCTACAGATT CCGACCTTGT GGTCACGATA ACGCTACCTC CTTGTCCTCT
     2401 AGGCAGTCCT TGGTAGATCT TGTCCCACCA GCTCAAATTC TTGTCCCATA CATCGTCCAT
     2461 AACTATCAAG TACCTTTTCC CCAAAAGATA CTGCTGGATT TTTCTCAGCA ATGTTCCAAT
     2521 ATCATCTCCA ACACTCGCGT CCCCCAAGTT CCTCAATATG CTTCTCATGA TCTGTTCTTC
     2581 AGTAAAAGTC TGGGACACAG ACACCCATAT CCTTCTTTCG AAACGATGTT CGATCTCTTT
     2641 GTCATTGAAC ACTTCTTGAG CGATTGTAGT TTTACCTAGC CCTCCCATCC CAACAAAGGC
     2701 CATTATCAAA AGCTGGCTGT CGTTTGATCG AAAGAGCCAC TCCTTGATTT TTCTTTTATC
     2761 TCCTTCCAAA CCAACCACCT GAGTATGATC ATACACCGGA GAGCTCCACC GATCAGTTCC
     2821 ATTATCTCTT CCAACATTAC TCGGCGTTAT GAATTCGAAA TAAGGCTCTA CTTGACTCTT
     2881 TATTTTCGTT ATCCTCTCAT TGATCTCTTG CAAACGTTTG CTTTTCTTGT ACTGCAACGG
     2941 AACCCGGGCA GGATGAAGCC GCGAAAGCCA CGCATTTGAT GACCTTTGCT CGTTCCCATC
     3001 ATCACCATCC GCGAGCTGAC AATCCACAAG TATATCCTCA GCTTCGTAAA CCAGTTCTCG
     3061 TAAATCCGCA ACAAGAGTAC GCAACGTTTC ATTAGTTCTC TTCTGCCTCT CTGCATCCTT
     3121 GAGAAAAGAC TGCATATATT TCAATTCGCT TTGCAAATCT TCCAACTGTT TTCGGTAGTC
     3181 CGATACGGTT CGGCCTTTTT CTTCTTCGAT GTTCAAGGTT TTCTCTAAAA ACACTGTTAC
     3241 AACAGCGTCC ACCATTAGAG ACCATTAAGG GCGAATTCGA CCCAGCTTTC TTGTACAAAG
     3301 TTGGCATTAT AAAAAATAAT TGCTCATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC
     3361 AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG
     3421 TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC
     3481 AAGATAAAAA TATATCATCA TGCCTCCTCT AGACCAGCCA GGACAGAAAT GCCTCGACTT
     3541 CGCTGCTGCC CAAGGTTGCC GGGTGACGCA CACCGTGGAA ACGGATGAAG GCACGAACCC
     3601 AGTGGACATA AGCCTGTTCG GTTCGTAAGC TGTAATGCAA GTAGCGTATG CGCTCACGCA
     3661 ACTGGTCCAG AACCTTGACC GAACGCAGCG GTGGTAACGG CGCAGTGGCG GTTTTCATGG
     3721 CTTGTTATGA CTGTTTTTTT GGGGTACAGT CTATGCCTCG GGCATCCAAG CAGCAAGCGC
     3781 GTTACGCCGT GGGTCGATGT TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGGGCA
     3841 GTCGCCCTAA AACAAAGTTA AACATCATGA GGGAAGCGGT GATCGCCGAA GTATCGACTC
     3901 AACTATCAGA GGTAGTTGGC GTCATCGAGC GCCATCTCGA ACCGACGTTG CTGGCCGTAC
     3961 ATTTGTACGG CTCCGCAGTG GATGGCGGCC TGAAGCCACA CAGTGATATT GATTTGCTGG
     4021 TTACGGTGAC CGTAAGGCTT GATGAAACAA CGCGGCGAGC TTTGATCAAC GACCTTTTGG
     4081 AAACTTCGGC TTCCCCTGGA GAGAGCGAGA TTCTCCGCGC TGTAGAAGTC ACCATTGTTG
     4141 TGCACGACGA CATCATTCCG TGGCGTTATC CAGCTAAGCG CGAACTGCAA TTTGGAGAAT
     4201 GGCAGCGCAA TGACATTCTT GCAGGTATCT TCGAGCCAGC CACGATCGAC ATTGATCTGG
     4261 CTATCTTGCT GACAAAAGCA AGAGAACATA GCGTTGCCTT GGTAGGTCCA GCGGCGGAGG
     4321 AACTCTTTGA TCCGGTTCCT GAACAGGATC TATTTGAGGC GCTAAATGAA ACCTTAACGC
     4381 TATGGAACTC GCCGCCCGAC TGGGCTGGCG ATGAGCGAAA TGTAGTGCTT ACGTTGTCCC
     4441 GCATTTGGTA CAGCGCAGTA ACCGGCAAAA TCGCGCCGAA GGATGTCGCT GCCGACTGGG
     4501 CAATGGAGCG CCTGCCGGCC CAGTATCAGC CCGTCATACT TGAAGCTAGA CAGGCTTATC
     4561 TTGGACAAGA AGAAGATCGC TTGGCCTCGC GCGCAGATCA GTTGGAAGAA TTTGTCCACT
     4621 ACGTGAAAGG CGAGATCACC AAGGTAGTCG GCAAATAACC CTCGAGCCAC CCATGACCAA
     4681 AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     4741 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     4801 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     4861 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
     4921 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     4981 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     5041 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     5101 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC
     5161 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     5221 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     5281 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
     5341 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
     5401 T
//