LOCUS       pZA16_pCR8_NbZAR1_L17E/ 5379 bp ds-DNA     circular     14-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(3374..3393)
                     /label="T7"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      complement(268..295)
                     /label="rrnB T2 transcription termination sequence"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3626..4636
                     /label="Spectinomycin resistance gene"
                     /ApEinfo_revcolor=#dd0000
                     /ApEinfo_fwdcolor=#dd0000
     misc_feature    3258..3357
                     /label="attL2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(569..668)
                     /label="attL1"
                     /ApEinfo_revcolor=#dd0000
                     /ApEinfo_fwdcolor=#dd0000
     terminator      complement(427..470)
                     /label="rrnB T1 transcription termination sequence"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     cds             complement(699..3233)
                     /label="NbZAR1_L17E/D481V_noSTOP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_binding    607..631
                     /label="GW1 priming site"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_binding    3295..3319
                     /label="GW2 priming site"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      4729..5317
                     /label="pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTCC GAATTCGCCC TTAATGGTCT CTCGAACCGT TCCTATGTTC TTCCTTCTTC
      721 CATAGACCCC CTCTAAACCC TGCATCTTCA ATTGGAAATG ACTCCAACTC AGGGCACCAG
      781 CTAACCTTGA GTATTCTTAA CGAAGGCATG ACTTGATGCA TTGCTGACCA TTCCAATCTC
      841 AAATCCGATA AAGATTCCAA CATTAAGCCC TCAATTTTCC AAGCAGTACT ACCATCACCC
      901 CAGAAGTTCT CGTGCATTTG TGTAATATCA CCAGAGATGA TTGATAAATA TCGCAACATG
      961 GGGGAGAGAT TGGGGTTAAG CCATTCAGGA CTTGTCTTAC TTGGATAAAA TTTGAGAATC
     1021 AGCTCGTGGA GTTGTCGTGG AGGATAAAGT CTACCAAGTT TTGTCGCAAG TCCATTATCT
     1081 TGACTGCCAA AGCAACTTAT TGTTAAAAAT TGAAGTTCTT GGAGATCTAC CAGTGCGTTC
     1141 CCCTCATCGT CTCCAATCTC CTCATTTTCG GTTAGTCTTA AACTGAGAGT TCTGAGTCGA
     1201 GTCAGGCTTC TGAGCTCAGC AATACGACAA CCTCCTGGTT GGCTTAATTT TGCAGGCTTA
     1261 AACCCAAGCA ATACTTGAAG ATTGGAAAGC CTACTTAATC CTTTTGGAAG GTACTCAAGG
     1321 GAACCACAGT TGTTCACATC CAAAACAGCA AGTTCCTCAA ATGTCAGAAC GCAAGAGGGG
     1381 AGCATTTTCA TATTTTGGCA ATAGCTGAAA TCCAAAATCT GTAATTTTTC AAGCTTGGAT
     1441 ATGGAAGCTG GAACTCCAAT CATTGGATGT GTATTGCTTA AACTGAGATA AGTGAGCTGC
     1501 TTAGCAGATC CAATTCCTTC CAACAAACTT GAAAGAGGCA CATCGAAAAT TGATTTTGAC
     1561 AGATCCAATA CTTGGAGGTG TCGACTCTTG CACAGCTTCT TGGCAATGTC GGAATGGATT
     1621 TTGTTAACTT CACCACTCTT GGTGGTTGTT AGCAGTGCTC GTAATTTTTG ATTGCTCAAT
     1681 AGTTGCTTCC AATTCATCTC ACTCTTAATA CCCAAATGCC GACAAGTTGC ATCAGCTGGG
     1741 GTGAAAAATG CATCATCCTC GGCTAATTTG ATCACCAAGT CACGAACCAT AACATGCATT
     1801 TTGCATGTGT GGATCACACC ATTGTAAGCC TTATCAACAA CTTCTATCAA ACACCGATTG
     1861 GATAGTTGTG AGAAACAATC TTCTCCCACT TCAGTCGATA ACCTACCACT TCTCAGAGGT
     1921 ATGAAACCTT CTCCAATCCA CCAACGGATC AGTTGGTCTT TTGGTATGAC ACAATCCTCA
     1981 GGAAAGAGTG AAAAACAGAG GAAACAGGAT TTCAAGTAAG GAGGGAGTTC ATCATAGCTC
     2041 AACTGTAGTG AGGCCATCAC TGAGTCATCA TTTTCTTTCA ATTCATCACG GAAATGATTT
     2101 GAAATACGCC TCCATTCGTG ATAGTAAGAT GGCTTGCAAA GCATCACTCC TCCTACTGCT
     2161 TTGATTGCTA AAGGAAGTCC CTTGCATTTT TCCACAATCT CCTTTCCCAC GTCCTCCAAC
     2221 TCGGGGAAAT GGCATTCACC TGCACTTCCT GCAAATGCAA TCTTCCGGAA TAGTAACCAA
     2281 CTGTAGTGCT CATTGAGGAA TTTTGGCCAA TGTATTCTTG CTTCTGTAAC TCCCATCTTG
     2341 CGAGCGACTA GCTCATTTCT CGTTGTTACA ATAACAGTGC TCCCATTTCC TTTGGGCAGT
     2401 CCAGTATAGA TTTTTTGCCA CCAGGCATTG TCCCAGCTCC AAACATCATC CATAACAATC
     2461 AAAAACCTCT TTCCCAAAAG GTACTGGTTT ATTTTTCTTA ACAGTTCACA CTGGTCATCA
     2521 CCAACACATG CATCTCCTAA ACTCCTCAAT ATGCTTCTCA TGACTTGTTC CTCAGTAAAT
     2581 GTTTGAGAAA CAGACACCCA AATCCTCCTC TCCAAGTGAT CCTCCACCCT TTTGTCATTG
     2641 AAAACTTTCT GAGCAAGAGT GGTTTTACCA AGTCCTCCCA TACCCACAAA TGCAATGGTA
     2701 AGTAAACCAT CTCTTGCTTC AAATAGCCAA TCCTTTATCT TCTCAGTGTC ACCTTCCAAA
     2761 CCAACTACCT GAGTGTGGTC ATAAAGGGAA GAAGTCCATC TTGACATTAG ATTATTGTGT
     2821 GCCTCCATAC TTCCTTCTTC GAGAAGTGGT ACTCCAAGAT ATGTTGAAAT GTTTTGCTTT
     2881 ATCTCCGAGA TTCTGTCATT GATTTTAGCA AGGCACTTCC CAGTTTGATG GCGAAGAGAG
     2941 AGCCTTTTAG GATGGAAGCA TGTAGTAGCC CGATCGCTAT CTGCAGATTG ATTCTGGCAG
     3001 TCCTCAAGTA TCTCTTCAGC TTCAAAGATT AAGTCTCGCA AACAGGACAT GACTCCTTTA
     3061 AGGGTGTTGT TTTTCCTCTT GAGCCTCTCT GCATCTTTGA GAAAGCTTTG CATGAATAGC
     3121 AGCTCGTTCT TCAGTTTTTC AAACTGCTGT CTGTATTTAG TAAGAAACCT ACTCTCCTCT
     3181 GTTTCAACAT GCAGAAGTTT CTCCAAGAAT ACAGTGACCA CCGCATCCAC CATTAGAGAC
     3241 CATTAAGGGC GAATTCGACC CAGCTTTCTT GTACAAAGTT GGCATTATAA AAAATAATTG
     3301 CTCATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTTGCCA
     3361 TCCAGCTGAT ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT
     3421 CTGGCCCGTG TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG
     3481 CCTCCTCTAG ACCAGCCAGG ACAGAAATGC CTCGACTTCG CTGCTGCCCA AGGTTGCCGG
     3541 GTGACGCACA CCGTGGAAAC GGATGAAGGC ACGAACCCAG TGGACATAAG CCTGTTCGGT
     3601 TCGTAAGCTG TAATGCAAGT AGCGTATGCG CTCACGCAAC TGGTCCAGAA CCTTGACCGA
     3661 ACGCAGCGGT GGTAACGGCG CAGTGGCGGT TTTCATGGCT TGTTATGACT GTTTTTTTGG
     3721 GGTACAGTCT ATGCCTCGGG CATCCAAGCA GCAAGCGCGT TACGCCGTGG GTCGATGTTT
     3781 GATGTTATGG AGCAGCAACG ATGTTACGCA GCAGGGCAGT CGCCCTAAAA CAAAGTTAAA
     3841 CATCATGAGG GAAGCGGTGA TCGCCGAAGT ATCGACTCAA CTATCAGAGG TAGTTGGCGT
     3901 CATCGAGCGC CATCTCGAAC CGACGTTGCT GGCCGTACAT TTGTACGGCT CCGCAGTGGA
     3961 TGGCGGCCTG AAGCCACACA GTGATATTGA TTTGCTGGTT ACGGTGACCG TAAGGCTTGA
     4021 TGAAACAACG CGGCGAGCTT TGATCAACGA CCTTTTGGAA ACTTCGGCTT CCCCTGGAGA
     4081 GAGCGAGATT CTCCGCGCTG TAGAAGTCAC CATTGTTGTG CACGACGACA TCATTCCGTG
     4141 GCGTTATCCA GCTAAGCGCG AACTGCAATT TGGAGAATGG CAGCGCAATG ACATTCTTGC
     4201 AGGTATCTTC GAGCCAGCCA CGATCGACAT TGATCTGGCT ATCTTGCTGA CAAAAGCAAG
     4261 AGAACATAGC GTTGCCTTGG TAGGTCCAGC GGCGGAGGAA CTCTTTGATC CGGTTCCTGA
     4321 ACAGGATCTA TTTGAGGCGC TAAATGAAAC CTTAACGCTA TGGAACTCGC CGCCCGACTG
     4381 GGCTGGCGAT GAGCGAAATG TAGTGCTTAC GTTGTCCCGC ATTTGGTACA GCGCAGTAAC
     4441 CGGCAAAATC GCGCCGAAGG ATGTCGCTGC CGACTGGGCA ATGGAGCGCC TGCCGGCCCA
     4501 GTATCAGCCC GTCATACTTG AAGCTAGACA GGCTTATCTT GGACAAGAAG AAGATCGCTT
     4561 GGCCTCGCGC GCAGATCAGT TGGAAGAATT TGTCCACTAC GTGAAAGGCG AGATCACCAA
     4621 GGTAGTCGGC AAATAACCCT CGAGCCACCC ATGACCAAAA TCCCTTAACG TGAGTTACGC
     4681 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT
     4741 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG
     4801 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA
     4861 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT
     4921 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA
     4981 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC
     5041 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT
     5101 GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA
     5161 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG
     5221 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT
     5281 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT
     5341 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT
//