LOCUS       Exported               10592 bp ds-DNA     circular SYN 14-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 16, 2020 from SnapGene Viewer 5.1.4.1
            https://www.snapgene.com
COMMENT     plix403-ccdb-G418 from 1 to 10358
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    45..60
                     /locus_tag="cPPT"
                     /label=cPPT
     misc_feature    208..535
                     /locus_tag="TetO promoter"
                     /label=TetO promoter
     CDS             543..1259
                     /locus_tag="EYFP"
                     /label=EYFP
     misc_feature    1290..1414
                     /locus_tag="attR1"
                     /label=attR1
     misc_feature    complement(1314..1414)
                     /locus_tag="attR2"
                     /label=attR2
     promoter        1439..1462
                     /locus_tag="lac_promoter"
                     /label=lac_promoter
     gene            1523..2182
                     /gene="CAT/CamR"
                     /locus_tag="CAT/CamR"
                     /label=CAT/CamR
     misc_feature    2524..2829
                     /locus_tag="ccdB"
                     /label=ccdB
                     /note="/translation=MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQL
                     DITAF 
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF 
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV 
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG 
                     A*"
     misc_feature    complement(2870..2977)
                     /locus_tag="attR1(1)"
                     /label=attR1
                     /label=attR1(1)
                     /note="/translation=MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQL
                     DITAF 
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF 
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV 
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG 
                     A*"
     misc_feature    2870..2970
                     /locus_tag="attR2(1)"
                     /label=attR2
                     /label=attR2(1)
                     /note="/translation=MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQL
                     DITAF 
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF 
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV 
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG 
                     A*"
     misc_feature    3080..3592
                     /locus_tag="PGK promoter"
                     /label=PGK promoter
     misc_feature    3601..4632
                     /locus_tag="Hygromycin Resistance"
                     /label=Hygromycin Resistance
     misc_feature    4645..4704
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    4705..5319
                     /locus_tag="TetR"
                     /label=TetR
     misc_feature    5320..5451
                     /locus_tag="VP64"
                     /label=VP64
     misc_feature    5488..6015
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="/translation=MAARRPRWAVANSGCSAGRAESSGREGAVREAGCGAVVW
                     ALFLP 
                     ARCSAFCKPPERTSAVGSLVDRITDLSPQQFTMTEYKPTVRLATRDDVPRAVRTLAAA 
                     FADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEA 
                     GAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSA 
                     VVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAE 
                     GRGSLLTCGDVEENPGPMSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTL 
                     YWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKV 
                     HLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEE 
                     RETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDF 
                     DLDMLPADALDDFDLDMLPADALDDFDLDMLPG*"
     misc_feature    6108..6129
                     /locus_tag="U3PPT"
                     /label=U3PPT
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     misc_feature    6108..6123
                     /locus_tag="cPPT(1)"
                     /label=cPPT
                     /label=cPPT(1)
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     misc_feature    6125..6177
                     /locus_tag="delta_U3"
                     /label=delta_U3
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     misc_feature    6178..6358
                     /locus_tag="HIV-1_5_LTR"
                     /label=HIV-1_5_LTR
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     misc_feature    6178..6358
                     /locus_tag="truncHIV-1_3_LTR"
                     /label=truncHIV-1_3_LTR
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     terminator      6433..6552
                     /locus_tag="SV40_PA_terminator"
                     /label=SV40_PA_terminator
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     misc_feature    6521..6540
                     /locus_tag="EBV_rev_primer"
                     /label=EBV_rev_primer
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     rep_origin      6570..6668
                     /locus_tag="SV40_origin"
                     /label=SV40_origin
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     misc_feature    6653..6672
                     /locus_tag="SV40pro_F_primer"
                     /label=SV40pro_F_primer
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     promoter        6728..6756
                     /locus_tag="AmpR_promoter"
                     /label=AmpR_promoter
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     gene            6798..7658
                     /gene="Ampicillin"
                     /locus_tag="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGV
                     VCTVF 
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK 
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS 
                     LWAASPPVSPRRPD*"
     rep_origin      7813..8432
                     /locus_tag="pBR322_origin"
                     /label=pBR322_origin
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        8741..8770
                     /locus_tag="lac_promoter(1)"
                     /label=lac_promoter
                     /label=lac_promoter(1)
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    8784..8806
                     /locus_tag="M13_pUC_rev_primer"
                     /label=M13_pUC_rev_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        8805..8823
                     /locus_tag="M13_reverse_primer"
                     /label=M13_reverse_primer
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     promoter        8840..8859
                     /locus_tag="T3_promoter"
                     /label=T3_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    9113..9293
                     /locus_tag="HIV-1_5_LTR(1)"
                     /label=HIV-1_5_LTR
                     /label=HIV-1_5_LTR(1)
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    9113..9293
                     /locus_tag="truncHIV-1_3_LTR(1)"
                     /label=truncHIV-1_3_LTR
                     /label=truncHIV-1_3_LTR(1)
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    9404..9448
                     /locus_tag="HIV-1_psi_pack"
                     /label=HIV-1_psi_pack
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
     misc_feature    9958..10191
                     /locus_tag="RRE"
                     /label=RRE
                     /note="/translation=MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAV
                     GIGAL 
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL 
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE 
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL 
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGQFSTSRQMAVFIHNFKRKGGIGGY 
                     SAGERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRDPLWPRIDMSSLLPISDRERM 
                     SSLLPISDRERCRVYSLSVIENVCRVYSLSVIENVCRVYSLSVIENVCRVYPYQ*"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      601 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      661 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      721 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
      781 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      841 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      901 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      961 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1021 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1081 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1141 actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     1201 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     1261 gggccggcca tatcagcggc tctggttcga caagtttgta caaaaaagct gaacgagaaa
     1321 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1381 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     1441 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1501 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1561 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1621 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1681 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1741 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1801 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1861 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1921 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     1981 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     2041 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     2101 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     2161 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
     2221 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2281 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2341 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2401 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2461 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     2521 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     2581 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     2641 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     2701 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     2761 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     2821 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     2881 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     2941 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgcagg
     3001 aaagccaatc ccaaacccac tcctaggtct cgacagtaca gcatagaccg gtccaccacc
     3061 accaccacca ctaaggatcc ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     3121 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     3181 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     3241 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     3301 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     3361 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     3421 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     3481 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     3541 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gcttaattaa
     3601 atggatagat ccggaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     3661 aagttcgaca gcgtttccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     3721 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     3781 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     3841 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     3901 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     3961 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     4021 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     4081 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     4141 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     4201 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     4261 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     4321 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     4381 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     4441 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     4501 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     4561 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     4621 agggcaaagg aacctgcagg tcccggtgcc gaaggtagag gttctctcct cacttgtggt
     4681 gatgttgaag aaaaccctgg tccaatgtct agactggaca agagcaaagt cataaacgga
     4741 gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     4801 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     4861 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     4921 gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca
     4981 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     5041 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     5101 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     5161 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     5221 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     5281 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt
     5341 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     5401 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag
     5461 gatcgatcca agatatcgta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5521 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5581 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5641 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5701 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5761 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5821 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     5881 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5941 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6001 gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg cgtccggact agaggtacct
     6061 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6121 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6181 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6241 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6301 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6361 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6421 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6481 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6541 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6601 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6661 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6721 gaggcctttc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6781 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6841 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6901 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6961 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7021 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7081 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7141 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7201 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7261 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7321 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7381 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7441 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7501 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7561 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7621 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7681 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7741 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7801 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7861 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7921 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7981 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8041 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8101 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8161 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8221 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8281 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8341 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8401 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8461 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8521 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8581 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8641 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     8701 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     8761 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     8821 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     8881 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     8941 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     9001 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     9061 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     9121 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9181 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9241 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     9301 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     9361 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     9421 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     9481 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     9541 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     9601 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     9661 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     9721 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     9781 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     9841 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     9901 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     9961 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    10021 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    10081 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    10141 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    10201 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    10261 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    10321 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    10381 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    10441 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    10501 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
    10561 gtttcagacc cacctcccaa ccccgagggg ac
//
