LOCUS       Exported               16245 bp ds-DNA     circular SYN 17-MAY-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pRDA_078
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 14, 2020 from SnapGene 3.3.3
            http://www.snapgene.com
COMMENT     pHKO_23 - pFUGW-U6-BsmBI (no 
            spacer)-chRNA(+85)-EFS-Cas9-FLAG-2A-Puro-WPRE 2
            note 5'LTR (R and U5 portions
            U3 was replaced by the CMV promoter) Added stuffer and re-named 
            pXPR_023.
FEATURES             Location/Qualifiers
     source          1..16245
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5087..10658
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          10038..10607
                     /label=Genscript 2xUGI
                     /note="Genscript 2xUGI"
                     /note="/created_by=jonlevy"
                     /note="/modified_by=jonlevy"
     misc_feature    252..257
                     /label=SpeI
                     /note="SpeI"
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=A^CTAGT"
                     /note="/annotation_group=ACTAGT"
     promoter        254..844
                     /label=CMV
                     /note="CMV"
     LTR             828..1016
                     /note="5'LTR"
     misc_feature    1067..1204
                     /note="Psi"
     misc_feature    1124..1127
                     /label=SD /note=""""SD
                     /note="SD /note=""""SD"
                     /note="splice donor"""
     misc_feature    1171..1535
                     /note="gag"
     misc_feature    1681..1922
                     /note="RRE"
     misc_feature    2440..2557
                     /note="CPPT"
     misc_feature    complement(2469..2493)
                     /note="Kermit"
     misc_feature    2602..2607
                     /label=KpnI
                     /note="KpnI"
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=GGTAC^C"
                     /note="/annotation_group=GGTACC"
     promoter        2608..2852
                     /label=hU6
                     /note="hU6"
     misc_feature    2834..2850
                     /note="Neon"
     misc_feature    2837..2857
                     /note="Argon"
     misc_binding    2853..4737
                     /label=Filler
                     /note="Filler"
     promoter        2853..2856
                     /label=hU6 [Split]
                     /note="hU6 [Split]"
     misc_feature    4738..4819
                     /note="chRNA"
     misc_feature    4820..4825
                     /label=EcoRI
                     /note="EcoRI"
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^AATTC"
                     /note="/annotation_group=GAATTC"
     misc_feature    4826..4831
                     /label=NheI
                     /note="NheI"
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^CTAGC"
                     /note="/annotation_group=GCTAGC"
     promoter        4832..5086
                     /label=EFS-NS
                     /note="EFS-NS"
     misc_feature    complement(4833..4857)
                     /note="Beaker"
     tRNA            5087..5118
                     /note="/created_by=lkoblan"
     tRNA            5087..5088
                     /label=20
                     /note="20"
                     /note="/created_by=lkoblan"
     misc_feature    5088..5093
                     /label=AgeI
                     /note="AgeI"
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=A^CCGGT"
                     /note="/annotation_group=ACCGGT"
     misc_feature    5094..10658
                     /label=TCTAGAGCGCTGCCACCATGGACAAGAAGTACAGCATCGGCCTGGACATC 
                     GGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGA 
                     AATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGC 
                     CCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGA 
                     AGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACG 
                     AGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGA 
                     AGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCC 
                     TACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCG 
                     ACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGG 
                     CCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTC 
                     ATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCG 
                     GCGTGGACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAA 
                     TCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCC 
                     CTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCA 
                     AACTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGAT 
                     CGGCGACCAGTACGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTG 
                     CTGAGCGACATCCTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTA 
                     TGATCAAGAGATACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCG 
                     GCAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTAC 
                     GCCGGCTACATTGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCA 
                     TCCTGGAAAAGATGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCT 
                     GCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGA 
                     GAGCTGCACGCCATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACC 
                     GGGAAAAGATCGAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGC 
                     CAGGGGAAACAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCC 
                     TGGAACTTCGAGGAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGA 
                     TGACCAACTTCGATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCT 
                     GTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGA 
                     ATGAGAAAGCCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGT 
                     TCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAAT 
                     CGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTG 
                     GGCACATACCACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGG 
                     AAAACGAGGACATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGA 
                     GATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAG 
                     CAGCTGAAGCGGCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACG 
                     GCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTT 
                     CGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGAC 
                     ATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATC 
                     TGGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGA 
                     GCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGA 
                     GAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCG 
                     AAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACAC 
                     CCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTAC 
                     GTGGACCAGGAACTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGC 
                     CTCAGAGCTTTCTGAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAA 
                     GAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAAC 
                     TACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGA 
                     CCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACA 
                     GCTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATG 
                     AACACTAAGTACGACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGA 
                     AGTCCAAGCTGGTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGAT 
                     CAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTG 
                     ATCAAAAAGTACCCTAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACG 
                     ACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTA 
                     CTTCTTCTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGC 
                     GAGATCCGGAAGCGGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGG 
                     ATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATAT 
                     CGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAG 
                     AGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCG 
                     GCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGG 
                     CAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGA 
                     AGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGA 
                     AAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCG 
                     GAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCC 
                     TCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCC 
                     CCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGA 
                     GATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTG 
                     GACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCG 
                     AGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTA 
                     CTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCC 
                     ACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGC 
                     TGGGAGGCGACAAGCGACCTGCCGCCACAAAGAAGGCTGGACAGGCTAAGAAGAAGAA 
                     AGATTACAAAGACGATGACGATAAG
                     /note="TCTAGAGCGCTGCCACCATGGACAAGAAGTACAGCATCGGCCTGGAC"
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=TCTAGAGCGCTGCCACCATGGACAAGAAGTACAGCA
                     TCGGC 
                     CTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGC 
                     CCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCT 
                     GATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGA 
                     ACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCT 
                     TCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTT 
                     CCTGGTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGAC 
                     GAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGG 
                     ACAGCACCGACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAA 
                     GTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGAC 
                     AAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCA 
                     ACGCCAGCGGCGTGGACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACG 
                     GCTGGAAAATCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAAC 
                     CTGATTGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCG 
                     AGGATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCT 
                     GGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGAC 
                     GCCATCCTGCTGAGCGACATCCTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGA 
                     GCGCCTCTATGATCAAGAGATACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGC 
                     TCTCGTGCGGCAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAG 
                     AACGGCTACGCCGGCTACATTGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCA 
                     TCAAGCCCATCCTGGAAAAGATGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAG 
                     AGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATC 
                     CACCTGGGAGAGCTGCACGCCATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGA 
                     AGGACAACCGGGAAAAGATCGAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGG 
                     CCCTCTGGCCAGGGGAAACAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACC 
                     ATCACCCCCTGGAACTTCGAGGAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCA 
                     TCGAGCGGATGACCAACTTCGATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCA 
                     CAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTG 
                     ACCGAGGGAATGAGAAAGCCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGG 
                     ACCTGCTGTTCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTT 
                     CAAGAAAATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAAC 
                     GCCTCCCTGGGCACATACCACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGG 
                     ACAATGAGGAAAACGAGGACATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGA 
                     GGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAA 
                     GTGATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGC 
                     TGATCAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTC 
                     CGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTT 
                     AAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACA 
                     TTGCCAATCTGGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGT 
                     GGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAA 
                     ATGGCCAGAGAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGA 
                     AGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGT 
                     GGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGG 
                     GATATGTACGTGGACCAGGAACTGGACATCAACCGGCTGTCCGACTACGATGTGGACC 
                     ATATCGTGCCTCAGAGCTTTCTGAAGGACGACTCCATCGACAACAAGGTGCTGACCAG 
                     AAGCGACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAG 
                     ATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCG 
                     ACAATCTGACCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCAT 
                     CAAGAGACAGCTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGAC 
                     TCCCGGATGAACACTAAGTACGACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGA 
                     TCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGT 
                     GCGCGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGA 
                     ACCGCCCTGATCAAAAAGTACCCTAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACA 
                     AGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTAC 
                     CGCCAAGTACTTCTTCTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTG 
                     GCCAACGGCGAGATCCGGAAGCGGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGA 
                     TCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCA 
                     AGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATC 
                     CTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGA 
                     AGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGT 
                     GGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATC 
                     ATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACA 
                     AAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGA 
                     AAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTG 
                     GCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGA 
                     AGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTA 
                     CCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGAC 
                     GCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAG 
                     AGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGC 
                     CTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTG 
                     CTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACC 
                     TGTCTCAGCTGGGAGGCGACAAGCGACCTGCCGCCACAAAGAAGGCTGGACAGGCTAA 
                     GAAGAAGAAAGATTACAAAGACGATGACGATAAG"
     tRNA            5100..5118
                     /label=*
                     /note="*"
                     /note="/created_by=lkoblan"
     CDS             5157..5840
                     /label=Genscript rAPOBEC1
                     /note="Genscript rAPOBEC1"
                     /note="/created_by=jonlevy"
     CDS             5841..5936
                     /label=Genscript BE4 linker
                     /note="Genscript BE4 linker"
                     /note="/Transferred_From=JL545-pCMV_BPNLS_rAPOBEC1(genscrip
                     t)_dl BE4_NpuN1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=jonlevy"
     CDS             5937..10037
                     /label=fz hSpCas9
                     /note="fz hSpCas9"
                     /note="hSpCas9"
                     /note="/modified_by=jonlevy"
     misc_feature    6227..6237
                     /label=PflMI
                     /note="PflMI"
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CCANNNN^NTGG"
                     /note="/annotation_group=CCANNNNNTGG"
     misc_feature    8408..8418
                     /label=PflMI
                     /note="PflMI"
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CCANNNN^NTGG"
                     /note="/annotation_group=CCANNNNNTGG"
     CDS             10608..10637
                     /label=UGI
                     /note="UGI"
                     /note="/created_by=User"
     misc_feature    10608..10637
                     /label=SV40 NLS
                     /note="SV40 NLS"
                     /note="/created_by=User"
     misc_feature    10638..10655
                     /label=SV40 NLS
                     /note="SV40 NLS"
                     /note="/created_by=User"
     misc_feature    10656..10658
                     /label=SV40 NLS
                     /note="SV40 NLS"
                     /note="/created_by=User"
     tRNA            complement(10656..10658)
                     /note="/created_by=lkoblan"
     tRNA            complement(10656..10658)
                     /note="/created_by=lkoblan"
     CDS             10659..10724
                     /codon_start=1
                     /label=P2A
                     /note="P2A"
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     misc_feature    10659..10664
                     /label=BamHI
                     /note="BamHI"
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=G^GATCC"
                     /note="/annotation_group=GGATCC"
     CDS             10725..11321
                     /codon_start=1
                     /label=Puro
                     /note="Puro"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_binding    11337..11925
                     /label=WPRE
                     /note="WPRE"
     LTR             11996..12231
                     /note="3' LTR SIN"
     polyA_signal    12255..12508
                     /label=BGH polyA
                     /note="BGH polyA"
     rep_origin      12532..12960
                     /label=F1 ori
                     /note="F1 ori"
     rep_origin      12987..13296
                     /label=SV40 rep
                     /note="SV40 rep"
     CDS             13417..13791
                     /codon_start=1
                     /label=BleomycinR
                     /note="BleomycinR"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13921..14051
                     /label=SV40 pA
                     /note="SV40 pA"
                     /note="SV40 polyadenylation signal"
     rep_origin      14437..15104
                     /label=pUC Ori
                     /note="pUC Ori"
                     /note="pUC ori"
     CDS             complement(15252..16112)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggag acggttgtaa atgagcacac
     2881 aaaatacaca tgctaaaata ttatattcta tgacctttat aaaatcaacc aaaatcttct
     2941 ttttaataac tttagtatca ataattagaa tttttatgtt cctttttgca aacttttaat
     3001 aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt cttcaccttt
     3061 aataatagct actccaccac ttgttcctaa gcggtcagct cctgcttcaa tcattttttg
     3121 agcatcttca aatgttctaa ctccaccagc tgctttaact aaagcattgt ctttaacaac
     3181 tgacttcatt agtttaacat cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt
     3241 tttaacaaat tctaatccag cttcaacagc tatttcacaa gctttcatga tttcttcttt
     3301 tgttaataaa caattttcca taatacattt aacaacatgt gatccagctg ctttttttac
     3361 agctttcatg tcttctaaaa ctaattcata atttttgtct tttaatgcac caatatttaa
     3421 taccatatca atttctgttg caccatcttt aattgcttca gaaacttcga atgcttttgt
     3481 agctgttgtg catgcaccta gaggaaaacc tacaacattt gttattccta catttgtgcc
     3541 ttttaataat tctttacaat agcttgttca atatgaatta acacaaactg ttgcaaaatc
     3601 aaattcaatt gcttcatcac ataattgttt aatttcagct ttcgtagcat cttgttttaa
     3661 taatgtgtga tctatatatt tgtttagttt cattttttct cctatatatt catttttaat
     3721 tttaattctt taataatttc gtctacttta actttagcgt tttgaacaga ttcaccaaca
     3781 cctataaaat aaatttttag tttaggttca gttccacttg ggcgaacagc aaatcatgac
     3841 ttatcttcta aataaaattt tagtaagtct tgtcctggca tattatacat tccatcgatg
     3901 tagtcttcaa cattaacaac tttaagtcca gcaatttgag ttaagggtgt tgctctcaat
     3961 gatttcatta atggttcaat ttttaatttc ttttcttctg gtttaaaatt caagtttaaa
     4021 gtgaaagtgt aatatgcacc catttcttta aataaatctt ctaaatagtc tactaatgtt
     4081 ttattttgtt ttttataaaa tcaagcagcc tctgctatta atatagaagc ttgtattcca
     4141 tctttatctc tagctgagtc atcaattaca tatccataac tttcttcata agcaaaaaca
     4201 aaatttaatc cgttatcttc ttctttagca atttctctac ccattcattt aaatccagtt
     4261 aaagttttta caatattaac tccatatttt tcatgagcga ttctatcacc caaatcactt
     4321 gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg ttttagattt
     4381 gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct tacaaaatga
     4441 ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat aataataata
     4501 tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc aaattctgga
     4561 tttggattta caacattttt aaatgtttca tcttcaaatg catgctcttc aacctcaata
     4621 acgttatatc ctgattcacg taatattttt ggggtaaatt tagttcctgt tccattaact
     4681 gcgctaaaaa taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt
     4741 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4801 accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg gaattggctc
     4861 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     4921 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     4981 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5041 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggtgccacca
     5101 tgaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtctcct
     5161 cagagactgg gcctgtcgcc gtcgatccaa ccctgcgccg ccggattgaa cctcacgagt
     5221 ttgaagtgtt ctttgacccc cgggagctga gaaaggagac atgcctgctg tacgagatca
     5281 actggggagg caggcactcc atctggaggc acacctctca gaacacaaat aagcacgtgg
     5341 aggtgaactt catcgagaag tttaccacag agcggtactt ctgccccaat accagatgta
     5401 gcatcacatg gtttctgagc tggtcccctt gcggagagtg tagcagggcc atcaccgagt
     5461 tcctgtccag atatccacac gtgacactgt ttatctacat cgccaggctg tatcaccacg
     5521 cagacccaag gaataggcag ggcctgcgcg atctgatcag ctccggcgtg accatccaga
     5581 tcatgacaga gcaggagtcc ggctactgct ggcggaactt cgtgaattat tctcctagca
     5641 acgaggccca ctggcctagg tacccacacc tgtgggtgcg cctgtacgtg ctggagctgt
     5701 attgcatcat cctgggcctg cccccttgtc tgaatatcct gcggagaaag cagccccagc
     5761 tgaccttctt tacaatcgcc ctgcagtctt gtcactatca gaggctgcca ccccacatcc
     5821 tgtgggccac aggcctgaag tctggaggat ctagcggagg atcctctggc agcgagacac
     5881 caggaacaag cgagtcagca acaccagaga gcagtggcgg cagcagcggc ggcagcgaca
     5941 agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg
     6001 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
     6061 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
     6121 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
     6181 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
     6241 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
     6301 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
     6361 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
     6421 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
     6481 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     6541 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     6601 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg
     6661 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     6721 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     6781 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     6841 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     6901 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     6961 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     7021 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     7081 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     7141 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     7201 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     7261 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     7321 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     7381 acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     7441 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg
     7501 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     7561 agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     7621 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     7681 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     7741 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     7801 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     7861 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     7921 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     7981 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     8041 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     8101 acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg
     8161 tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa
     8221 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     8281 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     8341 acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt
     8401 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc
     8461 ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga
     8521 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     8581 ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg
     8641 ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg
     8701 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt
     8761 acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg
     8821 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc
     8881 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta
     8941 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg
     9001 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca
     9061 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga
     9121 tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg
     9181 tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag
     9241 gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa
     9301 agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg
     9361 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc
     9421 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg
     9481 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc
     9541 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg
     9601 gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg
     9661 agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca
     9721 agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg
     9781 ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca
     9841 gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg
     9901 ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc
     9961 tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt
    10021 ctcagctggg aggtgacagc ggcgggagcg gcgggagcgg ggggagcact aatctgagcg
    10081 acatcattga gaaggagact gggaaacagc tggtcattca ggagtccatc ctgatgctgc
    10141 ctgaggaggt ggaggaagtg atcggcaaca agccagagtc tgacatcctg gtgcacaccg
    10201 cctacgacga gtccacagat gagaatgtga tgctgctgac ctctgacgcc cccgagtata
    10261 agccttgggc cctggtcatc caggattcta acggcgagaa taagatcaag atgctgagcg
    10321 gaggatccgg aggatctgga ggcagcacca acctgtctga catcatcgag aaggagacag
    10381 gcaagcagct ggtcatccag gagagcatcc tgatgctgcc cgaagaagtc gaagaagtga
    10441 tcggaaacaa gcctgagagc gatatcctgg tccataccgc ctacgacgag agtaccgacg
    10501 aaaatgtgat gctgctgaca tccgacgccc cagagtataa gccctgggct ctggtcatcc
    10561 aggattccaa cggagagaac aaaatcaaaa tgctgtctgg cggctcaaaa agaaccgccg
    10621 acggcagcga attcgagccc aagaagaaga ggaaagtcgg atccggcgca acaaacttct
    10681 ctctgctgaa acaagccgga gatgtcgaag agaatcctgg accgaccgag tacaagccca
    10741 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
    10801 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
    10861 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
    10921 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
    10981 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
    11041 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
    11101 ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
    11161 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
    11221 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
    11281 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aacgcgttaa gtcgacaatc
    11341 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
    11401 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
    11461 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
    11521 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
    11581 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
    11641 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
    11701 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
    11761 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
    11821 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
    11881 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
    11941 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
    12001 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
    12061 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    12121 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    12181 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
    12241 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
    12301 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
    12361 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
    12421 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
    12481 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
    12541 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    12601 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    12661 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    12721 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    12781 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    12841 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    12901 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    12961 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    13021 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    13081 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    13141 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    13201 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
    13261 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
    13321 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
    13381 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
    13441 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
    13501 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
    13561 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
    13621 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
    13681 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
    13741 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
    13801 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    13861 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    13921 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    13981 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    14041 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
    14101 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    14161 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    14221 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    14281 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    14341 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    14401 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    14461 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    14521 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    14581 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    14641 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    14701 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    14761 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    14821 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    14881 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    14941 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    15001 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    15061 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    15121 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    15181 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    15241 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    15301 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    15361 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    15421 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    15481 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    15541 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    15601 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    15661 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    15721 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    15781 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    15841 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    15901 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    15961 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    16021 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    16081 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    16141 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    16201 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
