LOCUS       pPSG830                 5717 bp    DNA              UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(4769..5532)
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_label="ColE1"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ColE1"
     misc_feature    1..680
                     /ApEinfo_revcolor="#00a1ee"
                     /ApEinfo_label="ScTDH3 Promoter"
                     /ApEinfo_fwdcolor="#00a1ee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ScTDH3 Promoter"
     misc_feature    1203..1211
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_label="PTS1"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="PTS1"
     misc_feature    complement(4621..4768)
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_label="KanR Promoter"
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="KanR Promoter"
     misc_feature    complement(3805..4620)
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="KanR"
     misc_feature    complement(3778..3804)
                     /ApEinfo_revcolor="#93ff35"
                     /ApEinfo_label="KanR Terminator"
                     /ApEinfo_fwdcolor="#93ff35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="KanR Terminator"
     misc_feature    join(1600..1605,1607..1610)
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsmBI"
     misc_feature    complement(join(5565..5570,5560..5563))
                     /ApEinfo_revcolor="#e72e00"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00ff15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsmBI"
     misc_feature    1888..2550
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_label="HIS3"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIS3"
     misc_feature    2554..2760
                     /ApEinfo_revcolor="#ff94ff"
                     /ApEinfo_label="HIS3 Terminator"
                     /ApEinfo_fwdcolor="#ff94ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIS3 Terminator"
     misc_feature    1651..1887
                     /ApEinfo_revcolor="#FF94FF"
                     /ApEinfo_label="HIS3 Promoter"
                     /ApEinfo_fwdcolor="#FF94FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIS3 Promoter"
     misc_feature    2551..2685
                     /ApEinfo_revcolor="#FF94FF"
                     /ApEinfo_label="HIS3 Terminator"
                     /ApEinfo_fwdcolor="#FF94FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIS3 Terminator"
     misc_feature    2765..3765
                     /ApEinfo_revcolor="#A66DE8"
                     /ApEinfo_label="2u"
                     /ApEinfo_fwdcolor="#A66DE8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="2u"
     misc_feature    join(5560..5713)
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_label="ConLS′"
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ConLS′"
     misc_feature    1457..1646
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_label="ConRE′"
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ConRE′"
     misc_feature    1607..1631
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_label="ConE scar"
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ConE scar"
     misc_feature    1..680
                     /ApEinfo_revcolor="#00A1EE"
                     /ApEinfo_label="Sc pTDH3"
                     /ApEinfo_fwdcolor="#00A1EE"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Sc pTDH3"
     misc_feature    1228..1452
                     /ApEinfo_revcolor="#FFADAD"
                     /ApEinfo_label="Sc tENO2"
                     /ApEinfo_fwdcolor="#FFADAD"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Sc tENO2"
     misc_feature    5571..5713
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_label="ConS"
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ConS"
     misc_feature    1457..1599
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_label="ConE"
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ConE"
     misc_feature    1607..1631
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_label="ConE scar"
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ConE scar"
     misc_feature    complement(4769..5532)
                     /ApEinfo_revcolor="#7F7F7F"
                     /ApEinfo_label="ColE1"
                     /ApEinfo_fwdcolor="#7F7F7F"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ColE1"
     misc_feature    1..680
                     /ApEinfo_revcolor="#00A1EE"
                     /ApEinfo_label="ScTDH3 Promoter"
                     /ApEinfo_fwdcolor="#00A1EE"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ScTDH3 Promoter"
     misc_feature    1228..1452
                     /ApEinfo_revcolor="#FF8EFF"
                     /ApEinfo_label="ScENO2 Terminator"
                     /ApEinfo_fwdcolor="#FF8EFF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="ScENO2 Terminator"
     misc_feature    complement(3805..4620)
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="KanR"
     misc_feature    complement(4621..4768)
                     /ApEinfo_revcolor="#93FF35"
                     /ApEinfo_label="KanR Promoter"
                     /ApEinfo_fwdcolor="#93FF35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="KanR Promoter"
     misc_feature    complement(3778..3804)
                     /ApEinfo_revcolor="#93FF35"
                     /ApEinfo_label="KanR Terminator"
                     /ApEinfo_fwdcolor="#93FF35"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="KanR Terminator"
     misc_feature    1651..1887
                     /ApEinfo_revcolor="#FF94FF"
                     /ApEinfo_label="HIS3 Promoter"
                     /ApEinfo_fwdcolor="#FF94FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIS3 Promoter"
     misc_feature    2551..2760
                     /ApEinfo_revcolor="#FF94FF"
                     /ApEinfo_label="HIS3 Terminator"
                     /ApEinfo_fwdcolor="#FF94FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="HIS3 Terminator"
     misc_feature    3728..3765
                     /ApEinfo_revcolor="#9A969B"
                     /ApEinfo_label="FRT site"
                     /ApEinfo_fwdcolor="#9A969B"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="FRT site"
     misc_feature    2765..3727
                     /ApEinfo_revcolor="#BDA661"
                     /ApEinfo_label="2micron"
                     /ApEinfo_fwdcolor="#BDA661"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="2micron"
     misc_feature    join(1600..1605,1607..1610)
                     /ApEinfo_revcolor="#E72E00"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00FF15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsmBI"
     misc_feature    complement(join(5565..5570,5560..5563))
                     /ApEinfo_revcolor="#E72E00"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#00FF15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="BsmBI"
     misc_feature    join(2167..2172,2175..2178)
                     /ApEinfo_revcolor="#E72E00"
                     /ApEinfo_label="Bbs1"
                     /ApEinfo_fwdcolor="#00FF15"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="Bbs1"
ORIGIN
        1 cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt
       61 agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt
      121 acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga
      181 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag
      241 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt
      301 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac
      361 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc
      421 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa
      481 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt
      541 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat
      601 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata
      661 aacacacata aacaaacaaa agatctatgg aagaaactgt aatgttatat cacgaattgg
      721 aagtcgcagc ctcagctgac gaagtatggt ctgtcgaagg ttctcctgaa ttaggtttac
      781 atttaccaga tttgttacca gctggaatat tcgcaaagtt tgagataact ggagatggtg
      841 gagaaggttc tattttggac atgacatttc cacctggtca attccctcat cattatagag
      901 agaagttcgt tttcttcgac cataagaata gatataagtt ggttgaacaa attgacggtg
      961 atttctttga tttgggagta acctactaca tggacactat tagggtcgtc gcaacaggtc
     1021 ctgattcttg tgtcatcaaa tcaacaactg aatatcatgt taaaccagaa tttgctaaga
     1081 ttgttaaacc tttaatcgat acagttccat tagccattat gtcagaggca atagctaagg
     1141 tagtattgga aaataagcac aaatcttctg agggatccgg ttcattggga agaggtagaa
     1201 gatccaaatt gtaataactc gagtggcagt gcttttaact aagaattatt agtcttttct
     1261 gcttattttt tcatcatagt ttagaacact ttatattaac gaatagttta tgaatctatt
     1321 taggtttaaa aattgataca gttttataag ttactttttc aaagactcgt gctgtctatt
     1381 gcataatgca ctggaagggg aaaaaaaagg tgcacacgcg tggctttttc ttgaatttgc
     1441 agtttgaaaa atgctggaaa tctgctcgtc agtggtgctc acactgacga atcatgtaca
     1501 gatcataccg atgactgcct ggcgactcac aactaagcaa gacagccgga accagcgccg
     1561 gcgaacacca ctgcatatat ggcatatcac aacagtccac gtctcaagca gttacagaga
     1621 tgttacgaac cactagtgca ctgcagtaca aacacagtcc tttcccgcaa ttttcttttt
     1681 ctattactct tggcctcctc tagtacactc tatatttttt tatgcctcgg taatgatttt
     1741 catttttttt tttccaccta gcggatgact cttttttttt cttagcgatt ggcattatca
     1801 cataatgaat tatacattat ataaagtaat gtgatttctt cgaagaatat actaaaaaat
     1861 gagcaggcaa gataaacgaa ggcaaagatg acagagcaga aagccctagt aaagcgtatt
     1921 acaaatgaaa ccaagattca gattgcgatc tctttaaagg gtggtcccct agcgatagag
     1981 cactcgatct tcccagaaaa agaggcagaa gcagtagcag aacaggccac acaatcgcaa
     2041 gtgattaacg tccacacagg tatagggttt ctggaccata tgatacatgc tctggccaag
     2101 cattccggct ggtcgctaat cgttgagtgc attggtgact tacacataga cgaccatcac
     2161 accactgaag actgcgggat tgctctcggt caagctttta aagaggccct aggggccgtg
     2221 cgtggagtaa aaaggtttgg atcaggattt gcgcctttgg atgaggcact ttccagagcg
     2281 gtggttgatc tttcgaacag gccgtacgca gttgtcgaac ttggtttgca aagggagaaa
     2341 gtaggtgatc tctcttgcga gatgatcccg cattttcttg aaagctttgc agaggctagc
     2401 agaattaccc tccacgttga ttgtctgcga ggcaagaatg atcatcaccg tagtgagagt
     2461 gcgttcaagg ctcttgcggt tgccataaga gaagccacct cgcccaatgg taccaacgat
     2521 gttccctcca ccaaaggtgt tcttatgtag tgacaccgat tatttaaagc tgctgcatac
     2581 gatatatata catgtgtata tatgtatacc tatgaatgtc agtaagtatg tatacgaaca
     2641 gtatgatact gaagatgaca aggtaatgca tcattctata cgtgtcattc tgaacgaggc
     2701 gcgctttcct tttttctttt tgctttttct ttttttttct cttgaactcg acggatcata
     2761 gagtaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
     2821 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
     2881 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
     2941 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
     3001 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
     3061 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
     3121 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
     3181 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
     3241 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
     3301 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
     3361 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
     3421 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
     3481 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
     3541 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
     3601 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
     3661 agtccggtgc gtttttggtt ttttgaaagt gcgtcatcag agcgcttttg gttttcaaaa
     3721 gcgctctgaa gttcctatac tttctagcta gagaatagga acttcccgag cggccgcgtg
     3781 ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa
     3841 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     3901 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     3961 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     4021 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc
     4081 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     4141 caaaccgtta ttcattcgtg attgcgcctg agcgaggcga aatacgcgat cgctgttaaa
     4201 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     4261 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     4321 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     4381 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     4441 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     4501 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     4561 atccatgttg gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa tatggctcat
     4621 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     4681 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa
     4741 tcgaactttt gctgagttga aggatcagtc atgaccaaaa tcccttaacg tgagttttcg
     4801 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4861 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4921 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4981 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5041 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5101 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5161 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5221 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5281 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5341 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5401 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5461 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5521 gtggataacc gtgcggccgc ccctgaattc gcatctagac tgatgagacg tggtagagcc
     5581 acaaacagcc ggtacaagca acgatctcca ggaccatctg aatcatgcgc ggatgacacg
     5641 aactcacgac ggcgatcaca gacattaacc cacagtacag acactgcgac aacgtggcaa
     5701 ttcgtcgcaa tacaacg
//
