LOCUS       Exported               11636 bp ds-DNA     circular SYN 13-AUG-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    cpf1-all in one
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   LC Manstein Han
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 20, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11636
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1298..5200
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5489..5560
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     30..53
                     /label=pCmR
     CDS             complement(113..763)
                     /codon_start=1
                     /product="chloramphenicol resistance protein"
                     /label=cmR
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     CDS             complement(764..1048)
                     /codon_start=1
                     /label=PcmR
                     /translation="AIVTLIIKIRKKRIFRYAQIL*KNTKDHIF*CGLLFFN*STH*FN
                     KRKLDKVGYF*NIYLCYSNIDF*KRIDSNEESRQVSLLNSL*IKI*EAYQ"
     misc_feature    1239..1255
                     /label=M13F
     primer_bind     complement(1279..1310)
                     /label=P43-Fncp1-bone-R
     primer_bind     1281..1323
                     /label=P43-Fncpf1-F
     CDS             complement(1298..5200)
                     /codon_start=1
                     /product="WP_003040289.1"
                     /label=FnCpf1-???
                     /label=FnCpf1 locus
                     /translation="MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAK
                     DYKKAKQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAKDT
                     IKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIELFKANSDITDID
                     EALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYRIVDDNLPKFLENKAKYESLK
                     DKAPEAINYEQIKKDLAEELTFDIDYKTSEVNQRVFSLDEVFEIANFNNYLNQSGITKF
                     NTIIGGKFVNGENTKRKGINEYINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVID
                     KLEDDSDVVTTMQSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSL
                     TDLSQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKL
                     ALEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLLQA
                     SAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVFEECYFELANIV
                     PLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEPDNTAILFIKDDKYYLGVMNK
                     KNNKIFDDKAIKENKGEGYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNH
                     STHTKNGSPQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEF
                     YREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALF
                     DERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEYDLIKDK
                     RFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILSIDRGERHLAYYTLVD
                     GKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVV
                     HEIAKLVIEYNAIVVFEDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKT
                     GGVLRAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQE
                     FFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSDKNHNWDTR
                     EVYPTKELEKLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTE
                     LDYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGKKLNL
                     VIKNEEYFEFVQNRNN"
     primer_bind     1298..1324
                     /label=cpf1-pZR-RH
     primer_bind     complement(1643..1671)
                     /label=168-4_L730-S-HWL-13F-R
     primer_bind     2009..2032
                     /label=K826-S-HWL-1235-F
     primer_bind     2798..2814
                     /label=K927-S-HWL-1235-F2
     primer_bind     3624..3644
                     /label=K1329-S-HWL-F3
     primer_bind     4119..4149
                     /label=cpf1-1000R
     primer_bind     4456..4477
                     /label=K1330-S-HWL-F
     primer_bind     4467..4493
                     /label=cpf1-700R
     primer_bind     4696..4724
                     /label=cpf-MR
     primer_bind     5052..5086
                     /label=cpf1-100R
     primer_bind     5052..5076
                     /label=pVeg-crRNA
     primer_bind     complement(5127..5155)
                     /label=cpf1-F
     primer_bind     complement(5160..5200)
                     /label=xcpf1-li-F
     primer_bind     complement(5162..5201)
                     /label=cpf1-pZR-FH
     misc_feature    complement(5201..5311)
                     /label=?P43-RBS-eGFP???
     misc_feature    5208..5215
                     /label=RBS
     mRNA            complement(5224..5237)
                     /gene="mpd"
                     /product="MPH"
                     /label=mpd mRNA
     gene            complement(5224..5237)
                     /gene="mpd"
                     /label=mpd
     misc_feature    5238..5311
                     /label=P43
     misc_feature    5242..5247
                     /label=regulatory region-10
                     /note="-35 and -10 signal of promoter controlled by sigma 
                     A"
     regulatory      5247..5256
                     /regulatory_class="minus_10_signal"
                     /note="-35 and -10 signal of promoter controlled by sigma 
                     B"
     misc_feature    5265..5270
                     /label=regulatory region-35
                     /note="-35 and -10 signal of promoter controlled by sigma 
                     A"
     regulatory      5272..5289
                     /regulatory_class="minus_35_signal"
                     /note="-35 and -10 signal of promoter controlled by sigma 
                     B"
     primer_bind     complement(5350..5393)
                     /label=sacA-Fncpf1-R
     primer_bind     5369..5415
                     /label=pVeg-sacAcrRNA-F
     promoter        5378..5442
                     /label=Pveg
     primer_bind     complement(5422..5461)
                     /label=all-N23-R
     primer_bind     complement(5425..5484)
                     /label=pHT-ganAcr-R
     misc_feature    5443..5461
                     /label=Fncpf1 scaffold
     primer_bind     5460..5516
                     /label=pHT-ganAcr-F
     misc_feature    5462..5484
                     /label=lacA-crRNA-1
     primer_bind     5484..5517
                     /label=all-N23-F
     terminator      5489..5560
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(5542..5574)
                     /label=pVeg-sacAcrRNA-R
     primer_bind     5550..5581
                     /label=sacA-Fncpf1-F
     primer_bind     complement(5559..5597)
                     /label=PHT-all-ganAT-b-R
     primer_bind     5573..5605
                     /label=PHT-all-ganAT-F
     misc_feature    5587..6086
                     /label=lacA-front
     primer_bind     5841..5866
                     /label=lacA-F-jin
     misc_feature    6087..6586
                     /label=lacA-end
     primer_bind     complement(6563..6598)
                     /label=PHT-all-ganAT-R
     primer_bind     6574..6608
                     /label=PHT-all-ganAT-b-F
     primer_bind     7482..7512
                     /label=pRepA
     CDS             7563..8597
                     /codon_start=1
                     /product="RepA"
                     /label=repA
                     /translation="MSEVNLKGNTDELVYYRQQTTGNKIARKRIKKGKEEVYYVAETEE
                     KIWTEEQIKNFSLDKFGTHIPYIEGHYTILNNYFFDFWGYFLGAEGIALYAHLTRYAYG
                     SKDFCFPSLQTIAKKMDKTPVTVRGYLKLLERYGFIWKVNVRNKTKDNTEESPIFKIRR
                     KVPLLSEELLNGNPNIEIPDDEEAHVKKALKKEKEGLPKVLKKEHDEFVKKMMDESETI
                     NIPEALQYDTMYEDILSKGEIRKEIKKQIPNPTTSFESISMTTEEEKVDSTLKSEMQNR
                     VSKPSFDTWFKNTKIKIENKNCLLLVPSEFAFEWIKKRYLETIKTVLEEAGYVFEKIEL
                     RKVQ"
     misc_binding    8804..8819
                     /label=RepA binding site
                     /bound_moiety="RepA"
     misc_binding    8897..8913
                     /label=RepA binding site
                     /bound_moiety="RepA"
     promoter        9713..9817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9818..10678
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10849..11437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
       61 tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa
      121 gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat
      181 aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt
      241 tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat
      301 ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag
      361 gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt
      421 ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt
      481 tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc
      541 cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga
      601 aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa
      661 tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct
      721 cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa
      781 tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc
      841 ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat
      901 ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaaaga ccacattaaa
      961 aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc cttttctttc
     1021 ttatcttgat aataagggta actattgccg atcgtccatt ccgacagcat cgccagtcac
     1081 tatggcgtgc tgctagcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     1141 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     1201 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     1261 cgagctcagg ccttaactca cattaattgc gttgcgctta gttattccta ttctgcacga
     1321 actcaaaata ctcttcattt ttgataacca aattgagttt tttgccctct tgattatttt
     1381 tgatcctacc tagtagcatc agacctttta gcccaatatg ataagcacca ttggcatcag
     1441 catcttgagg catatttttt ggcgcctgtc gcgaatcaaa gaaattgcca tttacatctg
     1501 ctactggtga aattagataa tctaactcag tacctgtttt tgagttacgc atttgtaaga
     1561 tagtatttag gacactagtt agcttagcaa aaaacttttt gtcgctctca ccgcaaatag
     1621 ctgctttgat acattcgcca tgcccatatt cgatagaata atcttttagc aatttctcca
     1681 actctttagt tggataaact tctcgagtat cccaattatg atttttatct gaatttctaa
     1741 agttaatcaa tctactccca aagctagcta tagtccactt gcctttggca gccttgtcac
     1801 caaagttttt ataatcaaaa ctaaactcaa aatagccctt atcaaggtta taacaaatct
     1861 tgtcaaactt actaaagaac tcttgagatt tgctgacact ttcatactta ggatataact
     1921 gatttacaaa accagttaca ggacaaattt ttgaagtaaa accagctggt acatagtaga
     1981 taatacctgt ttgtttaccc atctttttaa aagtctcaaa aggtgctgtt agctgataag
     2041 ctctaagcac tcccccagtt ttatcaaact cattatcttt gaaaactaga tagtttagtt
     2101 tctcaattag cattttttct aacttttgat agacctgctt ctctaccttg aaacgccctc
     2161 ttttaaatcc aaaatttaaa tcctcaaaaa ccacaatagc attatactct ataactagct
     2221 tagctatttc atgaactacc tgagatagat agccctcttt catctctttg atgttattta
     2281 tctttttcca gtctttccta gctgaatccc tatctttctc tattgcagca agcttatcat
     2341 ggtagtttgt tttcattcta tcattaccaa tgatgttgaa agtatcttgt ttgatgatat
     2401 tgcctttacc atctaccaaa gtatagtaag ctaaatgtct ttcacctcta tctatactta
     2461 atatatgaac atcatttgct ttttctttta gcaataaatt gatttcatca ttaaacttat
     2521 tagctccact agatttaaaa ttgattgtaa taggacagtg aaagaaaaac ttatcttcag
     2581 taaagcgttt atctttgatt aaatcatatt caaaaacact ctctttttta ggattatctt
     2641 tgtttttatt agctattgcc tctttagctg ggtgagtgat ttttttaggt attgattgtt
     2701 tacgataaaa aagctctgcc tcaccattta gcttataaac cacatcttga agatttctct
     2761 catcaaacag cgctttccaa tataaagtat gtagatttgg tcgccctttg ctataagctg
     2821 aaaaatcttt attatagatt tggaataggt acaatttacc ctgattaact acgctatcaa
     2881 tatagctctc tgatatattt tcaaaagtta gtttgtagcc ttgattttca acttctctat
     2941 aaaattcatc tatagaatta tatctttgag tatcagaaaa tctaaatcca aaatctttcc
     3001 actccggatg cttacttata gactgtttat aaaaatctat aaattttcgg caatcttcaa
     3061 tattaaactc aaatttttca tatccttttt gaggactacc attttttgta tgtgtggaat
     3121 gatttcttat tctaagtata tcttcactag gattataaaa ttttatagat ttagcagaaa
     3181 agaaaacctt aggtaacatt ttatttgcgc caggtaaaag tttataaaca atttttttat
     3241 aaccctcgcc tttattttct ttgatagctt tatcatcaaa tattttgtta tttttcttat
     3301 tcatcacacc cagataatat ttatcatctt tgataaataa aattgccgta ttgtcaggct
     3361 ctttattttt atcccaacca ttagccaaag tcgagttctc aaaattgagc ttaaatttct
     3421 catcactata tggcttttga gttatatagt ttctaatttt gttataaaga ggcactatat
     3481 tcgctagctc aaagtagcac tcctcaaata ctagataaaa atgctcatcc ttgtctaaaa
     3541 tatttgcctt atcttctgac tgactaatat gaaatatttt tagtttatgt aagagattat
     3601 tagtttgatc taaaagatcc ttgatagctt taacatcatc ttccgcacta gcttgaagta
     3661 ggtctttttt accttgattt tgatatttga tagatatctg tgccaaattg tctttgtttt
     3721 gagctatttc atcaaatatc atcggaatag ccgcaaagtt tgcaagtatt tcttcaaacc
     3781 tacactgttt atctatatct ctatgcttat taaattcttc taaggcaagc tttatagttt
     3841 ctagagataa gtattttgct ttttcagttt ttttggctat taattcttgc tctttcttac
     3901 tagggttatc aagattttta ggtgctattt gttgagttat atattctagt accgctgtac
     3961 caataacact ataatcatca aaaacttgtt gtgatagatc agtaagagat ttatcatttt
     4021 taaaataaat tttactcaaa tcaagttttt gagcttttaa atcatcaaat aataaagata
     4081 gtgtttcttt aatagatttt tcttctactg ttttaaaagc tgctatttgc tcataaaaac
     4141 tttgcatcgt tgtaactaca tcactatcat cttctaactt atcaattaca aaagatttag
     4201 attctgtatc acttaaaatt tgcttaaata aaacactcat tttatatttt ttgagtgttt
     4261 tatcatttat ttgctgtgag tatagattta tatattcatt tatacctttt ctctttgtat
     4321 tttcaccatt tacaaattta ccaccaataa tagtattaaa tttagtaata ccactttgat
     4381 ttagataatt attaaagttt gctatctcaa aaacttcatc aagtgaaaaa actctttgat
     4441 taacttcaga tgttttgtag tcaatatcaa aggttagctc ttctgccaaa tcttttttaa
     4501 tttgttcata gtttatagct tctggagctt tgtcttttaa actctcatac ttagctttat
     4561 tttctagaaa tttaggcaaa ttatcatcta ctatcctata aataatagat gtaggaatat
     4621 cattgctact ataaacattt tttctatttt catgaaaacc cttaaaataa gttgtccaac
     4681 ctttaaaaga tttgattatt tctaacgcct catctatatc tgtgatatca ctattggctt
     4741 taaatagttc tataccatta tccttagatt gctttagcca tagaattaaa tctgactctt
     4801 gccctttttt agcatcgata aggttttgat taaacaaatt cttaaatttc tctgagtcct
     4861 ttatatattc agatatttgt ttctttatcg tatcttttgc acttttaaaa tctttttgta
     4921 gattatcatc atcactcttt ttaagtttaa aataaacatc agaatagttt tgtaataaat
     4981 cttcgctaat acaaaccgaa cttaatatct cctctataaa aaactgatga tatttatcaa
     5041 ttatttgttt agcctttttg tagtctttag ctcttttctc atcatctaaa atcaaacctc
     5101 ttgcttttat gttttcaagt gttttaccct gtgggattaa ctcaaatctt agagttttac
     5161 ttaaactata tttattaaca aattcttgat aaattgacat tttttttcct ccttttctcg
     5221 agggtaccgc tatcacttta tattttacat aatcgcgcgc tttttttcac gcccatttct
     5281 aaaaatgtaa aataaatgta agaattttca gaaaaagcta acggaaaagg gagcggaaaa
     5341 gaatgatgta agcgtgaaaa attttttatc ttatcactta ttaacgttga tataatttaa
     5401 attttatttg acaaaaatgg gctcgtgttg tacaataaat gtaatttcta ctgttgtaga
     5461 tccttgttga cgttatgcca attgatttca aataaaacga aaggctcagt cgaaagactg
     5521 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctttgtctgc aactgaaaag
     5581 tttataagta gtgaaagtcg gccagcacct tcattccgtt cgctgtcgct ctttttccga
     5641 tttcgatggc tttttgaaca tcattgtttc ccccgccata cccgttgcca tttgaatcat
     5701 acgggtgatt ccagatgcgg acgcgaacat agttgacccc agcctgtttt aaagttgtaa
     5761 agatatcctg gcgttttccg tttgtattgt aaaaggtgac accgctgttt tccaaagcaa
     5821 taacgctgga tacatctgcc cctttgataa aatccttgtt catcccctct acttttttaa
     5881 caaaaagatc ctctgcccga agctctgaca cgtgcttctc cttctcaatg gcagcggcat
     5941 atcctgttga actacatgca ctccacacga tggcagccgc aaagaacatt ttcactttgc
     6001 ttttcatgat ttcatccccc caagacttag caagcgtttt cattctatag aaaaaagaat
     6061 ccgcccatat cgagcggagc atcagcattc tcctccttgt tctcttagcc cttcgttccg
     6121 cctgcggtta atccggacac aaaattcttt tgcagcataa taaagatgac ggcaaccggt
     6181 atgctgataa gcagggctcc ggccgcaaat gtcgtatagc tggcccccat gacatcattc
     6241 actaaattga atagaccgac gggcaatgta tatgattcag gcgttctcaa tatggttgag
     6301 gacagcacaa aatctccgag cggaccggta aagccgttca tggccacgac tgccgccatc
     6361 ggttttgata atggcagaat gatctggaag aagattctgg tgctgctggc tccgtcaatc
     6421 ttggcgcttt cgtctaaatc catcggaatg gaatccatgt accctttcat caaatacgta
     6481 ttcatcggga tcaggccgcc gatataaagc aagattagca gccagtggct attgatcatt
     6541 cccaagattt gcgccagcac aaacaaggca attaaagctg aaaactcctt acctggaaca
     6601 aatggttgaa acatacgagg ctaatatcgg cttattagga atagtccctg tactaataaa
     6661 atcaggtgga tcagttgatc agtatatttt ggacgaagct cggaaagaat ttggagatga
     6721 cttgcttaat tccacaatta aattaaggga aagaataaag cgatttgatg ttcaaggaat
     6781 cacggaagaa gatactcatg ataaagaagc tctaaaacta ttcaataacc ttacaatgga
     6841 attgatcgaa agggtggaag gttaatggta cgaaaattag gggatctacc tagaaagcca
     6901 caaggcgata ggtcaagctt aaagaaccct tacatggatc ttacagattc tgaaagtaaa
     6961 gaaacaacag aggttaaaca aacagaacca aaaagaaaaa aagcattgtt gaaaacaatg
     7021 aaagttgatg tttcaatcca taataagatt aaatcgctgc acgaaattct ggcagcatcc
     7081 gaagggaatt catattactt agaggatact attgagagag ctattgataa gatggttgag
     7141 acattacctg agagccaaaa aactttttat gaatatgaat taaaaaaaag aaccaacaaa
     7201 ggctgagaca gactccaaac gagtctgttt ttttaaaaaa aatattagga gcattgaata
     7261 tatattagag aattaagaaa gacatgggaa taaaaatatt ttaaatccag taaaaatatg
     7321 ataagattat ttcagaatat gaagaactct gtttgttttt gatgaaaaaa caaacaaaaa
     7381 aaatccacct aacggaatct caatttaact aacagcggcc aaactgagaa gttaaatttg
     7441 agaaggggaa aaggcggatt tatacttgta tttaactatc tccattttaa cattttatta
     7501 aaccccatac aagtgaaaat cctcttttac actgttcctt taggtgatcg cggagggaca
     7561 ttatgagtga agtaaaccta aaaggaaata cagatgaatt agtgtattat cgacagcaaa
     7621 ccactggaaa taaaatcgcc aggaagagaa tcaaaaaagg gaaagaagaa gtttattatg
     7681 ttgctgaaac ggaagagaag atatggacag aagagcaaat aaaaaacttt tctttagaca
     7741 aatttggtac gcatatacct tacatagaag gtcattatac aatcttaaat aattacttct
     7801 ttgatttttg gggctatttt ttaggtgctg aaggaattgc gctctatgct cacctaactc
     7861 gttatgcata cggcagcaaa gacttttgct ttcctagtct acaaacaatc gctaaaaaaa
     7921 tggacaagac tcctgttaca gttagaggct acttgaaact gcttgaaagg tacggtttta
     7981 tttggaaggt aaacgtccgt aataaaacca aggataacac agaggaatcc ccgattttta
     8041 agattagacg taaggttcct ttgctttcag aagaactttt aaatggaaac cctaatattg
     8101 aaattccaga tgacgaggaa gcacatgtaa agaaggcttt aaaaaaggaa aaagagggtc
     8161 ttccaaaggt tttgaaaaaa gagcacgatg aatttgttaa aaaaatgatg gatgagtcag
     8221 aaacaattaa tattccagag gccttacaat atgacacaat gtatgaagat atactcagta
     8281 aaggagaaat tcgaaaagaa atcaaaaaac aaatacctaa tcctacaaca tcttttgaga
     8341 gtatatcaat gacaactgaa gaggaaaaag tcgacagtac tttaaaaagc gaaatgcaaa
     8401 atcgtgtctc taagccttct tttgatacct ggtttaaaaa cactaagatc aaaattgaaa
     8461 ataaaaattg tttattactt gtaccgagtg aatttgcatt tgaatggatt aagaaaagat
     8521 atttagaaac aattaaaaca gtccttgaag aagctggata tgttttcgaa aaaatcgaac
     8581 taagaaaagt gcaataaact gctgaagtat ttcagcagtt ttttttattt agaaatagtg
     8641 aaaaaaatat aatcagggag gtatcaatat ttaatgagta ctgatttaaa tttatttaga
     8701 ctggaattaa taattaacac gtagactaat taaaatttaa tgagggataa agaggataca
     8761 aaaatattaa tttcaatccc tattaaattt taacaagggg gggattaaaa tttaattaga
     8821 ggtttatcca caagaaaaga ccctaataaa atttttacta gggttataac actgattaat
     8881 ttcttaatgg gggagggatt aaaatttaat gacaaagaaa acaatctttt aagaaaagct
     8941 tttaaaagat aataataaaa agagctttgc gattaagcaa aactctttac tttttcattg
     9001 acattatcaa attcatcgat ttcaaattgt tgttgtatca taaagttaat tctgttttgc
     9061 acaacctttt caggaatata aaacacatct gaggcttgtt ttataaactc agggtcgcta
     9121 aagtcaatgt aacgtagcat atgatatggt atagcttcca cccaagttag cctttctgct
     9181 tcttctgaat gtttttcata tacttccatg ggtatctcta aatgattttc ctcatgtagc
     9241 aaggtatgag caaaaagttt atggaattga tagttcctct ctttttcttc aactttttta
     9301 tctaaaacaa acactttaac atctgagtca atgtaagcat aagatgtttt tccagtcata
     9361 atttcaatcc caaatctttt agacagaaat tctggacgta aatcttttgg tgaaagaatt
     9421 tttttatgta gcaatatatc cgatacagca ccttctaaaa gcgttggtga atagggcatt
     9481 ttacctatct cctctcattt tgtggaataa aaatagtcat attcgtccat ctacctatcc
     9541 tattatcgaa cagttgaact ttttaatcaa ggatcagtcc tttttttcat tattcttaaa
     9601 ctgtgctctt aactttaaca actcgatttg tttttccaga tctcgagggt aactagcctc
     9661 gccgatcccg caagaggccc ggcagtcagg tggcactttt cggggaaatg tgcgcggaac
     9721 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     9781 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     9841 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     9901 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     9961 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10021 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
    10081 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10141 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    10201 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    10261 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    10321 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    10381 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    10441 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    10501 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    10561 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    10621 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    10681 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10741 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10801 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    10861 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    10921 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    10981 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11041 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11101 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11161 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    11221 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    11281 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    11341 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    11401 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    11461 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    11521 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    11581 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcatgc
//
