LOCUS       Exported                7103 bp ds-DNA     circular SYN 25-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAD123
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 20, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          7023..7094
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..33
                     /label=MCS
     misc_feature    39..46
                     /label=RBS
     CDS             54..770
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=gfpmut3a
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(84..112)
                     /label=GFP-R
     CDS             850..1101
                     /codon_start=1
                     /label=Importance
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQNKEYKKI
                     FTKNQFNNSLKHAGIDDLNNISFEQFLSLFNSYKLFNK"
     primer_bind     1144..1166
                     /label=pAD123-lacAT-F
     primer_bind     complement(1210..1242)
                     /label=pAD-sacAH-b-R
     primer_bind     1218..1252
                     /label=pAD-sacAH-F
     misc_feature    1229..2428
                     /label=sacA front end
     primer_bind     1496..1516
                     /label=???????
     primer_bind     complement(2410..2442)
                     /label=pAD-sacAH-R
     primer_bind     2418..2457
                     /label=pAD-sacAH-b-F
     misc_feature    complement(2497..3519)
                     /label=rep60 from pAT1060 origin
     misc_feature    3944..4594
                     /label=Cm(cat)
     misc_feature    4678..4814
                     /label=Feature 17
     misc_feature    4815..4955
                     /label=bom?from pBR322?
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5141..5729)
                     /direction=LEFT
                     /label=ori from pBR322 origin
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5900..6760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR(bla)
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6761..6865)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6797..6817
                     /label=Amp-F
     primer_bind     complement(6887..6926)
                     /label=pAD123-sacAcrT-b-R
     primer_bind     6897..6953
                     /label=pAD123-pVeg-sacAcrT-F
     promoter        6912..6976
                     /label=Pveg
     misc_feature    6977..6995
                     /label=Fncpf1 scaffold
     misc_feature    6996..7018
                     /label=crRNA-sacA??
     terminator      7023..7094
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(join(7076..7103,1..7))
                     /label=pAD123-pVeg-sacAcrT-R
     primer_bind     join(7081..7103,1..12)
                     /label=pAD123-sacAcrT-b-F
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agatttaaga aggagatata catatgagta
       61 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      121 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      181 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      241 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      301 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      361 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      421 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      481 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      541 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      601 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
      661 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
      721 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa atctcctgca
      781 ggcatgcaag cttgagtagg acaaatccgc cgagcttcga cgagattttc aggagctaag
      841 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
      901 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
      961 cagaacaaag aatacaagaa aatatttaca aaaaatcaat ttaacaattc cttaaaacat
     1021 gcaggaattg acgatttaaa caatattagc tttgaacaat tcttatctct tttcaatagc
     1081 tataaattat ttaataagta agttaaggga tgcataaact gcatccctta acttgttttt
     1141 cgtgtgccta ttttttgtga atcgctaaga aaccattatt atcatgacat taacctataa
     1201 aaataggcgt atcacgaggc cctttcgtct acataagtgt ccaaattccg acattcattt
     1261 cagatttccc ggtcgcactg gctgttactt catgatttcc cgggaaagga aaatatcttg
     1321 cactaaagac ctcttctccg ttattgataa agatttccac agatgacgcg tcgagaaaca
     1381 tgtgtacggt atgcaaatcc ttgatgcggc aatgtctcgc ttctgttctt tttccgtcaa
     1441 agctctttcg ttccagcgta acaatcccct cgtctttatg gaaggaaaac gtagccgttc
     1501 cgcggatact gattgaaaag ccagactccg tatgaatgtc ttccagtaga atctcagttc
     1561 tttcaggttt ttccactgga agcgcaccag atgatccatg catgttgatg tgtatttttt
     1621 tctcatttct gcgcatggct tttagctcag gcagcggctg ctgaatcagc ttctgtcctg
     1681 aaagcgtcag ctgtctcggc agcgtcatgc agtgaatcca gtggcagtca atggtcggat
     1741 gggacccttc atcctgatca ggcaccgcca tccatgcaaa taaaatccgc cttccctgat
     1801 cgtcttcaag tgtttgcggc gcgtaaaaaa atctccactc tgtcaggtta tcagaagcaa
     1861 acaacacagc ctgccctttc aaattctctg tttgcgcacc aatcaccata taccatgtgc
     1921 cttcatgctc ccataccttc ggatcgcgaa aatgcgccgt atatccttcc ggaaggcggg
     1981 cgacgacacc ctttttctca aaggacagcc cgtcgtcaga aacagcaagg cattgatacg
     2041 tttcccgatt tccatcctga tccctgacat ttcctgtgta aaaaagatag agccgatcgt
     2101 ctttcgtgac agcgctgccc gagtagcagc cgtttttatc aaaccaatca ctcggagcca
     2161 gcgcaatctc ttcccgcttc caattcacaa catcctgtgt cgtataatgc ccccaaaatt
     2221 ttgcgccgtg ccccgtctga aacggctgcc actgaaagaa tacatgatag ctgcccttcc
     2281 aataaatcac gccattcggg tcattcagca gcccaaccgg cggcatgata tgaaaatgct
     2341 ggcggtgcgg atcgctgtta gcgatgggct cttttttctc cacttcttca taagcccggc
     2401 gacgaagctc ctggtcatgt gctgtcatca aaacatattt caacacaata caaatgggtt
     2461 agttaaaaaa gcaggccttc taaaggtctg ctttttttat ttgattatgt aatttttaat
     2521 gccaggatgc caataagcca taacctcaaa tgcaccattt gcaacctcgt catcttcttc
     2581 ctcaatcttg accagatcgc cgtcctccgc atcaccaaga ttcagctctt tatgtatctc
     2641 cttcaaaatg ccaccgtatc caattaacct tcgagctgcc aacgcatcat ccaagtaaag
     2701 caccgtgttc agattgtctt cagtcacctt attaccgcgc acacaatccg tatccttaac
     2761 cggatattta gagatttcgc gaacagcttt ttgctccatc attgcgttcc gcacatcgtt
     2821 ttcaatctgt tcagcgtcaa tcttagcttt acctttcact cgacgaatat cgacaattgg
     2881 agtgtaatcc aatttcatcg cctttttcca aaggctcgtc cactccgcct gcttaatata
     2941 gtttttccca aaataatttt tccttactgg tatcaacaca tgaaaatgag gatgatatgt
     3001 atcttcttca tgatttttgg taatctctaa agctctgaaa aatccaagaa ccgaagtttt
     3061 tacttttttg tactggaaca gtttcctaaa gccttccatc atcgcagaaa tttgtggctt
     3121 cagccgttct ccctttacat ttcgaatcgt cagcgtgaga aaaatccatc cgcagccgta
     3181 ctgtctattg gcttcctcta cgatcaactt attgtgataa gcaattttta acgacctgcg
     3241 ccacgcacac atcggacata acctcacttt acaaaaatgg gcttgataca gttttaactt
     3301 gcccgtctcc gggtctctct taaacgaaag atactctgca caactaatta gtttttcagc
     3361 ctttttgcca tagtaaggtg ccccaatctt actctctaac gcttcgtaat gctccgccat
     3421 gaggttcgtc cgtctctttt tccccttcca atcccgcttt ttacctgttg cggttttatc
     3481 ttcgaggatg ctataatcat tttcagatga ataaatcaac aaaaaaactc cttctgagct
     3541 agttctctag cattctatta ttttgattcg acaccttaat aatagcagaa ggagttttta
     3601 cctgtcaaag aaccatcaaa cccttgatac acaaggcttt gacctaattt tgaaaaatga
     3661 tgttgtttct atatagtatc aagataagaa agaaaaggat ttttcgctac gctcaaatcc
     3721 tttaaaaaaa cacaaaagac cacatttttt aatgtggtct ttattcttca actaaagcac
     3781 ccattagttc aacaaacgaa aattggataa agtgggatat ttttaaaata tatatttatg
     3841 ttacagtaat attgactttt aaaaaaggat tgattctaat gaagaaagca gacaagtaag
     3901 cctcctaaat tcactttaga taaaaattta ggaggcatat caaatgaact ttaataaaat
     3961 tgatttagac aattggaaga gaaaagagat atttaatcat tatttgaacc aacaaacgac
     4021 ttttagtata accacagaaa ttgatattag tgttttatac cgaaacataa aacaagaagg
     4081 atataaattt taccctgcat ttattttctt agtgacaagg gtgataaact caaatacagc
     4141 ttttagaact ggttacaata gcgacggaga gttaggttat tgggataagt tagagccact
     4201 ttatacaatt tttgatggtg tatctaaaac attctctggt atttggactc ctgtaaagaa
     4261 tgacttcaaa gagttttatg atttatacct ttctgatgta gagaaatata atggttcggg
     4321 gaaattgttt cccaaaacac ctatacctga aaatgctttt tctctttcta ttattccatg
     4381 gacttcattt actgggttta acttaaatat caataataat agtaattacc ttctacccat
     4441 tattacagca ggaaaattca ttaataaagg taattcaata tatttaccgc tatctttaca
     4501 ggtacatcat tctgtttgtg atggttatca tgcaggattg tttatgaact ctattcagga
     4561 attgtcagat aggcctaatg actggctttt ataatatgag ataatgccga ctgtactttt
     4621 tacagtcggt tttctaatgt cactaacctg ccccgttagt cgccattcgc cagctgcctc
     4681 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     4741 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     4801 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     4861 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     4921 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     4981 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5041 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     5341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5581 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6181 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     6241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6481 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     6541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     6901 aaaccattat tttattaacg ttgatataat ttaaatttta tttgacaaaa atgggctcgt
     6961 gttgtacaat aaatgtaatt tctactgttg tagattcggc cgcctcgatt ataacaagat
     7021 ttcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     7081 cggtgaacgc tctccgtctt caa
//
