LOCUS       Exported                8689 bp ds-DNA     circular SYN 07-SEP-2017
DEFINITION  BI3_sW0111.G03.
ACCESSION   .
VERSION     .
KEYWORDS    pXPR_BRD502-sgCD45_1_1stMapBySW
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 21, 2020 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             466..699
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     LTR             521..622
                     /note="R"
                     /note="R"
     LTR             623..700
                     /note="U5"
                     /note="U5"
     polyA_signal    777..898
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      938..1073
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(1094..1112)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1122..1138)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1280..1735
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1761..1865
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             1866..2726
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2897..3485
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3773..3794
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3809..3839
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3847..3863
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3871..3887
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3908..3926
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3954..4180
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4181..4361
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     LTR             4183..4284
                     /note="R"
                     /note="R"
     LTR             4285..4362
                     /note="U5"
                     /note="U5"
     misc_feature    4408..4533
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4413..4550
                     /note="psi"
                     /note="psi"
     misc_feature    4517..4881
                     /note="gag"
                     /note="gag"
     misc_feature    5026..5259
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        5666..5906
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    6183..6300
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6359..6859
                     /note="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     protein_bind    complement(7324..7348)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7398..7418
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             7434..7976
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /note="p65"
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             8001..8372
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /note="HSF1"
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             8388..8441
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8469..376
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
ORIGIN
        1 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
       61 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
      121 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgttt cgcccgacca
      181 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
      241 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
      301 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
      361 ccgcaagccc ggtgccgcta gctgaacgcg ttaagtcgac tttaagacca atgacttaca
      421 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
      481 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
      541 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      601 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      661 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
      721 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
      781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      841 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      901 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      961 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     1021 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     1081 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     1141 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     1201 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     1261 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1321 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1381 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1441 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     1501 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     1561 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     1621 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     1681 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     1741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1861 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1921 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1981 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2041 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2101 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2161 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2221 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2281 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2341 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2401 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2461 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2521 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2581 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2641 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2701 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2761 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3241 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3481 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3541 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3601 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3661 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3721 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     3781 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     3841 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     3901 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     3961 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     4021 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     4081 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     4141 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     4201 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4261 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4321 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     4381 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     4441 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     4501 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     4561 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     4621 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     4681 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     4741 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     4801 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     4861 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     4921 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     4981 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     5041 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     5101 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     5161 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     5221 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     5281 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     5341 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     5401 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     5461 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     5521 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     5581 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     5641 cctcccaacc ccgaggggac ccagagaggg cctatttccc atgattcctt catatttgca
     5701 tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat
     5761 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
     5821 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
     5881 ggctttatat atcttgtgga aaggacgaaa caccggttgt tctaagtcag tagaagttta
     5941 agagctatgc tgcgaatacg agggagcaga cgatatggcg tcgctcctct ccacgagagc
     6001 atatgggctc cgtggtctcg tacaccatca gggtacgtat cagacaccat cagggtctgc
     6061 tcgtattcgc agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg
     6121 caccgagtcg gtgctttttt tgaattctag atcttgagac aaatggcagt attcatccac
     6181 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     6241 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     6301 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccgggtt
     6361 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     6421 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     6481 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     6541 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     6601 tcttactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     6661 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     6721 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     6781 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     6841 caccgacctc tctccccagg gggatccaac ttaagcttgc caccatgtcg cggaggcggc
     6901 acagctacga gaacgatggt ggacaacctc acaaaaggag gaagacgtct atgctagcct
     6961 ccaaaaccat cgttctttcg gtcggcgagg ctactcgcac tctgactgag atccagtcca
     7021 ccgcagaccg tcagatcttc gaagagaagg tcgggcctct ggtgggtcgg ctgcgcctca
     7081 cggcttcgct ccgtcaaaac ggagccaaga ccgcgtatcg agtcaaccta aaactggatc
     7141 aggcggacgt cgttgattcc ggacttccga aagtgcgcta cactcaggta tggtcgcacg
     7201 acgtgacaat cgttgcgaat agcaccgagg cctcgcgcaa atcgttgtac gatttgacca
     7261 agtccctcgt cgcgacctcg caggtcgaag atcttgtcgt caaccttgtg ccgctgggcc
     7321 gttcttcaac tttcttgtac aaagtgggcg gaggaggtgg aagcggagga ggaggaagcg
     7381 gaggaggagg tagcggacct aagaaaaaga ggaaggtggc ggccgctggt accccttcag
     7441 ggcagatcag caaccaggcc ctggctctgg cccctagctc cgctccagtg ctggcccaga
     7501 ctatggtgcc ctctagtgct atggtgcctc tggcccagcc acctgctcca gcccctgtgc
     7561 tgaccccagg accaccccag tcactgagcg ctccagtgcc caagtctaca caggccggcg
     7621 aggggactct gagtgaagct ctgctgcacc tgcagttcga cgctgatgag gacctgggag
     7681 ctctgctggg gaacagcacc gatcccggag tgttcacaga tctggcctcc gtggacaact
     7741 ctgagtttca gcagctgctg aatcagggcg tgtccatgtc tcatagtaca gccgaaccaa
     7801 tgctgatgga gtaccccgaa gccattaccc ggctggtgac cggcagccag cggccccccg
     7861 accccgctcc aactcccctg ggaaccagcg gcctgcctaa tgggctgtcc ggagatgaag
     7921 acttctcaag catcgctgat atggacttta gtgccctgct gtcacagatt tcctctagtg
     7981 ggcagggagg aggtggaagc ggcttcagcg tggacaccag tgccctgctg gacctgttca
     8041 gcccctcggt gaccgtgccc gacatgagcc tgcctgacct tgacagcagc ctggccagta
     8101 tccaagagct cctgtctccc caggagcccc ccaggcctcc cgaggcagag aacagcagcc
     8161 cggattcagg gaagcagctg gtgcactaca cagcgcagcc gctgttcctg ctggaccccg
     8221 gctccgtgga caccgggagc aacgacctgc cggtgctgtt tgagctggga gagggctcct
     8281 acttctccga aggggacggc ttcgccgagg accccaccat ctccctgctg acaggctcgg
     8341 agcctcccaa agccaaggac cccactgtct ccgatatcgg cagtggagag ggcagaggaa
     8401 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aggtaccaca gatccaccgg
     8461 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     8521 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     8581 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     8641 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggc
//
