LOCUS       Exported                5633 bp ds-DNA     circular SYN 15-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCR-Hyg
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, November 14, 2018 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        8..396
                     /note="hsp60 promoter"
     misc_feature    455..490
                     /note="Direct Repeats"
     misc_feature    527..562
                     /note="Direct Repeats"
     terminator      579..625
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             757..1755
                     /codon_start=1
                     /note="Hyg"
                     /translation="VTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVPP
                     VLRVPGESTNPVLVGEPGSVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGRGE
                     LRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLTGNT
                     VLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPRFVHG
                     DLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQWK
                     RTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGA"
     rep_origin      1919..4672
                     /note="pAL5000ts"
                     /note="mycobacterial origin"
     rep_origin      4811..5399
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tctagacggt gaccacaacg cgcccgcttt gatcggggac gtctgcggcc gaccatttac
       61 gggtcttgtt gtcgttggcg gtcatgggcc gaacatactc acccggatcg gagggccgag
      121 gacaaggtcg aacgaggggc atgacccggt gcggggcttc ttgcactcgg cataggcgag
      181 tgctaagaat aacgttggca ctcgcgaccg gtgagtgcta ggtcgggacg gtgaggccag
      241 gcccgtcgtc gcagcgagtg gcagcgagga caacttgagc cgtccgtcgc gggcactgcg
      301 cccggccagc gtaagtagcg gggttgccgt cacccggtga cccccgtttc atccccgatc
      361 cggaggaatc acttcgcaat ggccaagaca attgcggatc cagctgcaga atttggcgcc
      421 agaggcactt cgaggctgat ttaggcaaaa acgggtctaa gaactttaaa taatttctac
      481 tgttgtagat tccgtctccg gcctccagta gtattcggcg aagcttgtct aagaacttta
      541 aataatttct actgttgtag atatcgactg ccaggcatca aataaaacga aaggctcagt
      601 cgaaagactg ggcctttcgt tttatgccat catggccgcg ggctagctta tcaccagccc
      661 gtcatcgtca acgcctgatc cgcggtgcgg acaggccgtg tcgcgaccgg ccgtgcggaa
      721 ttaagccggc ccgtaccctg tgaatagagg tccgctgtga cacaagaatc cctgttactt
      781 ctcgaccgta ttgattcgga tgattcctac gcgagcctgc ggaacgacca ggaattctgg
      841 gagccgctgg cccgccgagc cctggaggag ctcgggctgc cggtgccgcc ggtgctgcgg
      901 gtgcccggcg agagcaccaa ccccgtactg gtcggcgagc ccggctcggt gatcaagctg
      961 ttcggcgagc actggtgcgg tccggagagc ctcgcgtcgg agtcggaggc gtacgcggtc
     1021 ctggcggacg ccccggtgcc ggtgccccgc ctcctcggcc gcggcgagct gcggcccggc
     1081 accggagcct ggccgtggcc ctacctggtg atgagccgga tgaccggcac cacctggcgg
     1141 tccgcgatgg acggcacgac cgaccggaac gcgctgctcg ccctggcccg cgaactcggc
     1201 cgggtgctcg gccggctgca cagggtgccg ctgaccggga acaccgtgct caccccccat
     1261 tccgaggtct tcccggaact gctgcgggaa cgccgcgcgg cgaccgtcga ggaccaccgc
     1321 gggtggggct acctctcgcc ccggctgctg gaccgcctgg aggactggct gccggacgtg
     1381 gacacgctgc tggccggccg cgaaccccgg ttcgtccacg gcgacctgca cgggaccaac
     1441 atcttcgtgg acctggccgc gaccgaggtc accgggatcg tcgacttcac cgacgtctat
     1501 gcgggagact cccgctacag cctggtgcaa ctgcatctca acgccttccg gggcgaccgc
     1561 gagatcctgg ccgcgctgct cgacggggcg cagtggaagc ggaccgagga cttcgcccgc
     1621 gaactgctcg ccttcacctt cctgcacgac ttcgaggtgt tcgaggagac cccgctggat
     1681 ctctccggct tcaccgatcc ggaggaactg gcgcagttcc tctgggggcc gccggacacc
     1741 gcccccggcg cctgacgccc cgggccgccc ggcgccgccc ccggcccccg gcggccatcg
     1801 acccaagtac cgccacctaa catacgttgc gtcgagagac gaaggcacca cgattaccgg
     1861 cgacgatgat ctattgccgg tctacaccga cgctggggtg attgcgtttg ctccgggaac
     1921 accgatcgct tcgttcaaaa tcggcggaag ataccccgtg cgtgcggtgc tgaaggaccg
     1981 caaagatgtt ccgtacggca ccgaggtctg caagtacggg atgaaatccg gcgagacctg
     2041 tgggccggtc gttgtggctg ccaagtacac gatcaccgct cagctgcgca ccgtacatgg
     2101 cgactcaggc gcgccgctgt accgcaagaa ccgcgacggc acagccgatt tgatcggcat
     2161 cgcttcgagc gttaacgagg acagtcgcac gacgaagttc ttctggatcg cgcccgtgct
     2221 ggaagcactc aacctcgaag cgtgtggttg cggagccatc tagcaaccac acgaaacatg
     2281 cgcaacgaac cgcgcaacga acaacgccta gaactggccc tagatgagct gactcgtatc
     2341 gttggtaaac ctagtttgac cagcatgttt taactacgtt cggtgagctg tcaacggggc
     2401 ctgtaacggc acaacgaacc gtgcaacgag agtggccacg gatgccacca caggcactac
     2461 aacggagttc gccacgtaca tcaccacaac caccgattct ggcggtgagc tccccgatat
     2521 tcagcggaaa tggcttggta tcgaccaaga ttcgtagaac cccgtctcgt ctggctggta
     2581 ttcaaaacgg gcgcaacgaa acacgcaacg agacaggcat ggcccaaacc agaaaactag
     2641 cgtctaccag gacttttacc tgtccgaccc gttgcaacgg aaccccccac ggaacccccg
     2701 cgacacccgc tccccaattg cgttagaaca gcggtggatt gtcggcttcg ttgtgggcct
     2761 tttgagccgc ttcctgttct gccgcacgct ctttcctcgc ccgatagccg agtcgcttaa
     2821 cggtgtccag atgcagcccg aaatgtttgg ccgtttgcgg ccaagagtgg ccctcgtcgt
     2881 cgtgataggc gcggatgcgt tcgcggcgtg cagcctgctc ggcgagccac tcgctgcgtt
     2941 cctgcgccac gagccggacg acgtggcgtt cggatagtcc ggtgattcga gcgccttcgg
     3001 cggcggtcac gcgccgcttt ttgcggacag tcggctgccg gttgtagccg tcgctgtagc
     3061 cgtcgctgta gccgtcgctc atagcaatgc ctccatggct gacgcggact ttgcgcgccg
     3121 cgcaactgtg ctcgccgccg tgcgcgctgc tgcgcccttc cgcgagatgg ccgactggcg
     3181 cgcactgagt gtggcctcgt agaccacgat cccgtccgcc caaatgcgcg acttggttgt
     3241 gatccaacgc caaatgctgt tggcgatggc gcggacctcg ctgtccggta gcggtccggg
     3301 acacacgtcg ttgcacggga attcggcgtt tcgcgcgtgg cactcggcat agatcgcgcg
     3361 gccgagtccg tccacgttcc gggtcggcag gtagatccgc atgagggcgg gacgataggc
     3421 ccacaacctg acggaatcga acagtgcgca attccgccct agcggcgtcg gagccgcttt
     3481 gtacgtggtc tgctgacgcc agcgcggcgg tggcatgttc gcgccgagct cggcctcgat
     3541 gtggctgagt gtgtagagat ctgagtggag ccattccgtt tcccaggcga tgtggccggg
     3601 gtttttggtc atgaggcctg agtaactgcg gtcgccgtcg acggcgcgcc gaaggccttc
     3661 ggcgcacgcc gccatgtatg cgagcggctt acgccgcgcg tattcggtgc gtggaacagg
     3721 ggcgttgagt gcccacactg cgtgtgcgtg gccgttggcg cgattgccca cgatcgcgtt
     3781 gggcagcgga tgggaccccc gggcgctgag cgctcggagc gctgcgtctg gatggtctac
     3841 gtccacgacc agcaggtttg ccagcgctgt tgggttcgcc tcgatgtacc ggcggcctag
     3901 ggccgacgcg cggctttggc ggtagatccc ctcgagcaga tcgtcgcttg ccagcggcca
     3961 gtacggcagc cagagctgct caaattcgtc ggcgacgtgg ctcacgcttg gtagtagacc
     4021 acgattaatc accggtgtat ggtccgacac gagctccaag tcagatattt cgctgagggg
     4081 ccaccccaca actgcacact cccccgctct cccgtcgagc cctggtggtg gaacaccagc
     4141 gacagccgag cacccccaac cacctgtacc aaccaggagg aacacatgcg tcgtttcgag
     4201 gacgtttccg ggccgctgag agccgctgtg gcggccgtac acgccgcctt agacccgtta
     4261 gaccccctgc cgcctgaatg cgcgggtacg agccacacag cgcccgaact tacggagctg
     4321 gtgggctcac ctggctttat ggcgtacgaa tcggctgtgt gcgacctgtt gggcgaggtg
     4381 aggtacgcgc tactcacgct ggcaagggcg acacagccgc cccaccgagc ccgcacggcc
     4441 gcgcgcggtg tcaacaaccg ggtgagtcgt gcacaccagc aggtgttcga ggcttggctc
     4501 gaagtgcagg acatcgtggc gaacgccgcc cgatgagccg cgccttacgc tggctgccag
     4561 ccgttcgcgg gctggttggt gcagcgcgtc gagcggttag aggccctgcg gtgttccacc
     4621 accgcaggcc tcgccctttt taaggctgaa tttgcttgtc tccgaatcca actggcttgt
     4681 ccaagggtgt atctacgctt aatccaaagt tcaaacgagg ggattacaca tgaccaactt
     4741 cgataacgtt ctcggctcga tactcgttcc actgagcgtc agaccccgta gaaaagatca
     4801 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4861 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4921 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4981 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5041 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5101 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5161 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5221 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5281 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5341 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5401 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5461 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5521 ataccgctcg ccgcagccga acgaccgagc gcagcgagcg gccctagttg gcatatggga
     5581 ttcaccttta tgttgataag aaataaaaga aaatgccaat aggatatcgg cat
//
