LOCUS       Exported                4107 bp ds-DNA     circular SYN 21-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1k-c6p4aw6
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 21, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(268..295)
     terminator      complement(427..470)
     primer_bind     536..553
                     /standard_name="M13-fwd"
                     /label=M13-fwd
     misc_feature    603..726
                     /standard_name="attR4"
                     /label=attR4
     misc_recomb     complement(603..726)
                     /standard_name="attR4"
                     /label=attR4
     misc_feature    727..889
                     /standard_name="Ter_HSP"
                     /label=Ter_HSP
     misc_feature    1542..1985
                     /standard_name="pUC18 p35s"
                     /label=pUC18 p35s
     misc_feature    1543..1985
                     /standard_name="P35S"
                     /label=P35S
     misc_feature    2003..2168
                     /standard_name="DP1(Mitochondria)"
                     /label=DP1(Mitochondria)
     misc_feature    2004..2168
                     /standard_name="Mt DIPS"
                     /label=Mt DIPS
     misc_recomb     2170..2293
                     /standard_name="attR3"
                     /label=attR3
     misc_feature    2170..2292
                     /standard_name="attR3"
                     /label=attR3
     misc_recomb     complement(2170..2190)
                     /standard_name="attB3"
                     /label=attB3
     primer_bind     complement(2348..2367)
                     /standard_name="T7"
                     /label=T7
     primer_bind     complement(2368..2388)
                     /standard_name="M13-rev"
                     /label=M13-rev
     CDS             2501..3307
                     /standard_name="KanR"
                     /label=KanR
     rep_origin      3435..4063
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccct acaggtcact aataccatct aagtagttga
      601 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
      661 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttctataca
      721 aagttgtgaa tatgaagatg aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg
      781 cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt tttctaatat
      841 taaatgaatg taagatctca ttataatgaa taaacaaatg tttctataat ccattgtgaa
      901 tgttttgttg gatctcttct gcagcatata actactgtat gtgctatggt atggactatg
      961 gaatatgatt aaagataaga tgggctcata gagtaaaacg aggcgaggga cctataaacc
     1021 tcccttcatc atgctatttc atgatctatt ttataaaata aagatgtaga aaaaagtaag
     1081 cgtaataacc gcaaaacaaa tgatttaaaa catggcacat aatgaggaga ttaagttcgg
     1141 tttacgttta ttttagtact aattgtaacg tgagactacg tatcgggaat cgcctaatta
     1201 aagcattaat gcgaacctga ttagattcac cgaccctcct atcgtgtcga cctttctgtt
     1261 tcttagaatt ttttggtagt ctatgtacta ataatgtcag cttcgtattt atttcataag
     1321 caatttgcat ttgcaatttg ttttttactt ttatttttat tgtattgtgg aatgtggact
     1381 cgtaccaaca tgaagttata taccaccaaa aaaattacag ttagtcaaaa gattcacgag
     1441 tgagagctac ttatgattgt cttttacgta tatgtctaat tgtctatttg ctcaataatc
     1501 tttgtacttt cttttgtcgt tgataaatct agaggatccc ccctcagaag accagagggc
     1561 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
     1621 tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca
     1681 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg
     1741 acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
     1801 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
     1861 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacag gcttcttgag
     1921 atccttcaac aattaccaac aacaacaaac aacaaacaac attacaatta ctatttacaa
     1981 ttacagtcga cccacgcgta agataatttc cccaatttct cagagagact caaaatcacc
     2041 accaccacca aatcgccgcc atgtttaaac aagcttctcg tctcctctcc cgatctgtcg
     2101 ccgccgcatc ttccaaatcg gtgacgactc gtgccttttc aacggaactt ccatcgacgc
     2161 tcgattccac aactttgtat aataaagttg aacgagaaac gtaaaatgat ataaatatca
     2221 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     2281 tccagtcact atgaatcaac tacttagatg gtattagtga cctgtactgc agggcggccg
     2341 cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2401 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     2461 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     2521 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2581 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     2641 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2701 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2761 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     2821 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2881 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2941 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     3001 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     3061 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     3121 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     3181 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     3241 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3301 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     3361 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3421 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3481 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3541 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3601 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3661 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3721 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3781 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3841 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3901 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3961 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4021 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4081 cttttgctgg ccttttgctc acatgtt
//
